• 한국농공학회지
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• 제30권2호
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• pp.31-43
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• 1988

The Purpose of this study is to develop the rainfall-delayed response model (RDR Model) which influences the baseflow proportion of rivers as a result of the antecedent precipitation of the previous several months. The assesment of accurate baseflows in the rivers is one of the most important elements for the planning of seasonal water supply for agriculture, water resources development, hydrological studies for the availability of water and design criteria for various irrigation facilities. The Palukan river gauging site which is located in the Pulukan catchment on Bali Island, Indonesia was selected to develop this model. The basic data which has been used comprises the available historic flow records at 19 hydrologic gauging stations and 77 rainfall stations on Bali Island in the study. The methology adopted for the derivation of the RDR model was the water balance equation which is commonly used for any natural catcbment ie.P=R+(catchment losses) -R+(ET+DP+DSM+DGW). The catchment losses consist of evapotranspiration, deep percolation. change in soil moisture, and change in groundwater storage. The catchment areal rainfall has been generated by applying the combination method of Thiessen polygon and Isohyetal lines in the studies. The results obtained from the studies may be summarized as follows ; 1. The rainfall-runoff relationship derived from the water balance equation is as shown below, assuming a relationship of the form Y=AX+B. Finally these two equations for the annual runoff were derived ; ARO$_1$=0.855 ARF-821, ARF>=l,400mm ARO$_2$=0.290ARF- 33, ARF<1,400mm 2. It was found that the correction of observed precipitation by a combination of Thiessen polygons and Isohyetal lines gave good correlation. 3. Analysis of historic flow data and rainfall, shows that surface runoff and base flow are 52 % and 48% (equivalent to 59.4 mm) of the annual runoff, respectively. 4. Among the eight trial RDR models run, Model C provided the correlation with historic flow data. The number of months over which baseflow is distributed and the relative proportions of rainfall contributing in each month, were estimated by performing several trial runs using data for the Pulukan catchment These resulted in a value for N of 4 months with contributing proportions of 0.45, 0.50, 0.03 and 0.02. Thus the baseflow in any month is given by : P$_1$(n) =0.45 P(n) +0.50 P(n-I ) +0.03 P(n-$_2$) +0.02 P(n-$_3$) 5. The RDR model test gave estimated flows within +3.4 % and -1.0 % of the observed flows. 6. In the case of 3 consecutive no rain months, it was verified that 2.8 % of the dependable annual flow will be carried over the following year and 5.8 % of the potential annual baseflow will be transfered to the next year as a result of the rainfall-delayed response. The results of evaluating the pefformance of the RDR Model was generally satisfactory.

• Journal of Microbiology and Biotechnology
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• 제29권6호
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• pp.923-932
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• 2019

Current strategies of strain improvement processes are mainly focused on enhancing the synthetic pathways of the products. However, excessive metabolic flux often creates metabolic imbalances, which lead to growth retardation and ultimately limit the yield of the product. To solve this problem, we applied a dynamic regulation strategy to produce $\text\tiny{L}$-phenylalanine ($\text\tiny{L}$-Phe) in Escherichia coli. First, we constructed a series of Phe-induced promoters that exhibited different strengths through modification of the promoter region of tyrP. Then, two engineered promoters were separately introduced into a Phe-producing strain xllp1 to dynamically control the expression level of one pathway enzyme AroK. Batch fermentation results of the strain xllp3 showed that the titer of Phe reached 61.3 g/l at 48 h, representing a titer of 1.36-fold of the strain xllp1 (45.0 g/l). Moreover, the $\text\tiny{L}$-Phe yields on glucose of xllp3 (0.22 g/g) were also greatly improved, with an increase of 1.22-fold in comparison with the xllp1 (0.18 g/g). In summary, we successfully improved the titer of Phe by using dynamic regulation of one key enzyme and this strategy can be applied for improving the performance of strains producing other aromatic amino acids and derived compounds.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1109-1117
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• 2014

Chlorogenic acid and hydroxylcinnamoyl shikimates are major dietary phenolics as well as antioxidants, with recently discovered biological, activities including protection against chemotheraphy side effects and prevention of cardiovascular disease and cancer. Certain fruits and vegetables produce these compounds, although a microbial system can also be utilized for synthesis of chlorogenic acid and hydroxylcinnamoyl shikimates. In this study, we engineered Escherichia coli to produce chlorogenic acid and p-coumaroyl shikimates from glucose. For the synthesis of chlorogenic acid, two E. coli strains were used; one strain for the synthesis of caffeic acid from glucose and the other strain for the synthesis of chlorogenic acid from caffeic acid and quinic acid. The final yield of chlorogenic acid using this approach was approximately 78 mg/l. To synthesize p-coumaroyl shikimates, wild-type E. coli as well as several mutants were tested. Mutant E. coli carrying deletions in three genes (tyrR, pheA, and aroL) produced 236 mg/l of p-coumaroyl shikimates.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1442-1448
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• 2015

The flavonoid apigenin and its O-methyl derivative, genkwanin, have various biological activities and can be sourced from some vegetables and fruits. Microorganisms are an alternative for the synthesis of flavonoids. Here, to synthesize genkwanin from tyrosine, we first synthesized apigenin from p-coumaric acid using four genes (4CL, CHS, CHI, and FNS) in Escherichia coli. After optimization of different combinations of constructs, the yield of apigenin was increased from 13 mg/l to 30 mg/l. By introducing two additional genes (TAL and POMT7) into an apigenin-producing E. coli strain, we were able to synthesize 7-O-methyl apigenin (genkwanin) from tyrosine. In addition, the tyrosine content in E. coli was modulated by overexpressing aroG and tyrA. The engineered E. coli strain synthesized approximately 41 mg/l genkwanin.

• Asian-Australasian Journal of Animal Sciences
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• 제3권2호
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• pp.85-89
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• 1990

Sixteen Harnai lambs were used in a completely randomized design to study the nutritive value of fourwing saltbush as a winter maintenance browse in comparison to native range grazing with or without protein and energy supplementation at Tomagh Range Livestock Research Station, in Loralai District, Balochistan. The animals were divided into four groups of four lambs each. These four groups were assigned four treatments at random: fourwing saltbush grazing alone, range grazing plus lucerne hay (100 g/head/day), range grazing plus barley grain (100 g/head/day) and range grazing alone for ten weeks. The results indicate that the two range grazing plus supplementation treatments produced weight gains which were not significantly different from each other (p < 0.05). Both of these treatments yielded significantly higher weight gains (p < 0.05) than did range grazing alone except for the last week of the study. Fourwing saltbush grazing provided cumulative weight gains at 3, 4, 6, 7 and 8 weeks which were not significantly different from the range grazing plus lucerne hay treatment and gained an average of 6 percent in body weight over the 10 week period of study. The carrying capacity for sheep of a mature stand of fourwing saltbush was approximately 20 Sheep-kg-days (SKD) of grazing per cubic meter of foliage. Results of this study suggest that under fourwing saltbush grazing alone, lambs do not only maintain their body weights but can also gain weight in winter.

• Journal of Microbiology and Biotechnology
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• 제30권10호
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• pp.1574-1582
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• 2020

Flavonoids have diverse biological functions in human health. All flavonoids contain a common 2-phenyl chromone structure (C6-C3-C6) as a scaffold. Hence, in using such a scaffold, plenty of high-value-added flavonoids can be synthesized by chemical or biological catalyzation approaches. (2S)-Naringenin is one of the most commonly used flavonoid scaffolds. However, biosynthesizing (2S)-naringenin has been restricted not only by low production but also by the expensive precursors and inducers that are used. Herein, we established an induction-free system to de novo biosynthesize (2S)-naringenin in Escherichia coli. The tyrosine synthesis pathway was enhanced by overexpressing feedback inhibition-resistant genes (aroG^fbr and tyrA^fbr) and knocking out a repressor gene (tyrR). After optimizing the fermentation medium and conditions, we found that glycerol, glucose, fatty acids, potassium acetate, temperature, and initial pH are important for producing (2S)-naringenin. Using the optimum fermentation medium and conditions, our best strain, Nar-17LM1, could produce 588 mg/l (2S)-naringenin from glucose in a 5-L bioreactor, the highest titer reported to date in E. coli.

• 한국균학회지
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• 제36권2호
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• pp.189-195
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• 2008

Aspergillus nidulans는 빛이 없는 조건에서는 유성분화가 주로 일어나고 빛이 있는 조건에서는 유성분화가 억제되고 대신 무성분화가 유도된다. 빛에 의해서 유성분화가 억제되는 것은 빛에 반응하여 유성 또는 무성분화를 조절하는 유전자가 있다는 것을 시사한다. 따라서 빛에 의해서 조절되는 유전자를 연구하기 위하여 광 조건하에서 유성분화를 하는 silA98 돌연변이를 분리하였으며, 이를 보완하는 유전자를 분리 및 분석하고자 A. nidulans의 AMA-NotI genomic library로부터 silA98 돌연변이를 상보하는 유전자 silA를 분리하였다. silA 유전자의 예상 ORF는 2,388 bp의 염기로 구성되어지고 795개의 아미노산을 암호화하고 있었다. 이 유전자는 Saccharomyces cerevisiae의 ARO80 유전자와 상동성을 보이며 SilA 단백질의 N 말단에는 약 51.9%의 상동성을 가지는 ${Zn_2}{Cys_6}$ motif를 지니고 있었다. silA 유전자 결손돌연변이주는 광 존재 하에서뿐만 아니라 고농도의 sorbitol에서도 유성분화가 유도되었다. 이는 silA 유전자가 빛과 고삼투 조건에서 유성분화를 억제하는 조절과정에 관여하고 있음을 의미한다. silA 유전자를 niiA promoter로 과다 발현시켰을 때의 형질은 야생형과 큰 차이를 보이지 않았다.

• 한국도서관정보학회지
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• 제2권
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• pp.1-21
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• 1975

Thc purposc of this .study is for ri~aking a model of building a library collcction dcnizn~cd on th.3 curriculum through thc inter-comparison of 15 standards for collcgc ant1 anivcrsily libraries in 5 countries (U. S. A.. Great Ikitain, Canada, Japan, and Icorca) Thc rcsult of thcscl colnpsrisons is as follow.. (1) Qu;~nlitalivc scope: of thc cull(:ction: (r/ Standards for a minimal collcction arc presented to about 100,000 vols (20-30 vols per student) for a ncw university and about 40,000 vols (40 vols pcr studcnt) for a nrnr collcge. $)$ Standards for a working collcction arc prescnted to 70-80 vols per student for a univer:aity and 50-60 vols per student for a college. $$ Standard for an intcnsive rescc~rch collcction is presented to morc than 100 vols p::r studcnt. (2) Stop: of the annual incrc;lsc cf thc collection: (i: Standard for a minimal collcction is ~~rescntcdto 1.4-2 vols per studcnt. $$ Standard for an intensive rrscarch or working collection is pcrsented to 4-5 vols pcr studcnt. (3) Scope of thc anni.1a1 budgetary standards: In Grcat I3ritain and U.S. A.. th-re aro assigned to 1 . 5 ~ 2 % of the collcge and university cxpcnditurr for books. $,$ In our country, it is prcscntcd to 3.5% of thc collcge and university expenditure for books, whcn my calculating by thc various factor analysis.

• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.8-12
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• 2005

Response in a modified reciprocal recurrent selection scheme for egg production was evaluated considering variable family sizes and genetic correlation between purebred and crossbred half sisters. The criteria of selection of purebred breeders included pullet's own performance, purebred full and half sisters and crossbred half sister's performance. Heritability of egg production of crossbreds (aggregate genotype) and purebred's was assumed to be 0.2 and genetic correlation between purebred and crossbred half sisters ($r_{pc}$) as 0.1, 0.2, 0.3, 0.4, 0.5, 1.0, -0.1, -0.2, -0.3, -0.4, -0.5 and -1.0. Number of dams per sire to produce purebred and crossbred progenies assumed to be 5, 6, 7, 8, while number of purebred female progeny ($N_p$) and crossbred progeny ($N_c$) per dam were considered to be 3, 4, 5 and 6 in each case. Considering phenotypic variance as unity, selection indices were constructed for different combinations of dams and progeny for each value of $r_{pc}$. Following selection index theory, response in crossbred and purebred for egg production was computed. Results indicated that response in crossbreds depended mainly on crossbred family size and also on magnitude of$r_{pc}$ irrespective of its direction, and response was greater with large crossbred family size than the purebred families. Correlated response in purebreds depends both on magnitude and direction of $r_{pc}$ and was expected to be greater with large purebred family size only. Inclusion of purebred information increased the accuracy of selection for crossbred response for higher magnitude of$r_{pc}$ irrespective of its direction. Present results indicate that desirable response in both crossbred and purebred performance is a function of $r_{pc}$ and family sizes. The ratio of crossbred and purebred family sizes can be optimized depending on the objective of improving the performance of crossbreds and/or of purebreds.

• KSBB Journal
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• 제22권1호
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• pp.62-65
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• 2007

대사 공학을 이용한 생산 균주 개발의 핵심 기술은 원하는 대사산물을 과량으로 얻기 위하여 기존의 대사회로에서 제거, 증폭, 변경을 시켜야 할 유전자를 선정하는 것이다. 대사조절 분석 기법은 대사 흐름이 특정 효소의 활성에 따라 어떻게 변하는지를 예측하는 기술이다. 본 논문에서는 대장균의 threonine 생합성 효소 반응 kinetic model과 대사조절 분석 기법을 이용하여 threonine 생합성 flux를 가장 효과적으로 증가시키기 위하여 활성 증가가 필요한 효소가 aspartate semialdehyde dehyogenase라는 것을 밝혔다. 이러한 결과를 확인하기 위하여 asd가 과발현된 vector와 threonine 생합성 경로의 다른 효소인 aspartate kinase를 coding하는 thrA를 과발현 시키는 vector를 제작하여 threonine 생산 균주인 TF5015에 형질전환하여 threonine 농도를 측정하였다. Flask 배양결과 대사조절 분석 기법으로 확인된 유전자 asd를 과발현시킬 경우가 생합성 경로의 다른 유전자를 과발현시킨 경우보다 더 높은 threonine 농도의 증가를 보였다. 이러한 연구 결과들은 효소 반응 kinetic model과 대사조절 분석 기법을 이용하여 원하는 product를 효율적으로 생산할 수 있는 생산 균주를 제작할 수 있게 할 것이다.