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Studies on Conditions of the Gluconic Acid Production by a Mold isolated from the Soil of Seoul Area (Aspergillus SP. 균주에 의한 Gluconic Acid 생산조건에 관한 연구)

  • 양호석;김동훈;양한철
    • Microbiology and Biotechnology Letters
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    • v.8 no.2
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    • pp.93-102
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    • 1980
  • Fungi which were capable of producing gluconic acid were isolated from soil and tree leave samples, which had been collected in Seoul ana its vicinity. Among the 19 strains isolated, a strain named arbitrarily KUF-O4 was selected as a test strain chiefty because of its efficiency in gluconic acid production. The strain was identified as an Aspergillus sp. through its morphological properties. Optimum conditions for the gluconic acid production of KUF-O4 were investigated. The results obtained are as follows. 1. An incubation period of at least 30 hours was required for a good yield of gluconic acid. 2. A medium containing 10% glucose needed at least 3 % CaCo$_3$to maintain the optimum pH for the production of gluconic acid during fermentation. 3. As a carbon source, glucose was the most effective one among the carbon sources tested. 4. As a nitrogen source, an ammonium salt was more effective than any other form of nitrogen compounds. 5. As mineral source, a small amount of both KH$_2$PO$_4$and MgSO$_4$was found to be necessary to increase the efficiency of the gluconic acid production.

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Portal's Liability for User Reply to News Article, Provided by the News Media -A Critical Analysis on 2005 GaHap64571 of Seoul Central District Court- (언론사로부터 전재 받은 뉴스기사의 댓글에 대한 포털의 작위의무 -서울중앙지법 2005가합64571 판결에 대한 비판적 고찰-)

  • Kim, Gyong-Ho
    • Korean journal of communication and information
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    • v.42
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    • pp.140-167
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    • 2008
  • This study analyzes the legal reasoning of Seoul Central District Court, which imposed legal liability on portals for posting defamatory user replies to news articles, written and provided by the news media, onto their 'News Windows'. Saddling portals with the burden of verifying the facts associated in news articles and imposing the legal obligation as a publisher entail a grave risk of impairment of free flow of information and freedom of expression. Of course, it would ultimately result in tightening up private censorship of information which the Constitution does not allow, and funker keep portals from posting even news articles in which expressed views and opinions are lawful. When judging whether portals should assume liability fur libelous user replies to news articles, it is necessary to distinguish the territory under the direct authority of portals from cafes and bulletin boards managed by third parties. In addition, imposing legal liability above the level of common carrier should be limited to the cases; when portals arbitrarily change the contents of news articles or when the articles portals changed contain libelous contents. Even if those conditions are met, the altered contents should obviously constitute libel. Only in the presence of proof that portals knew the illegality of news articles and did not take proper steps including deleting those replies, should portals not be considered as an accomplice. Nor should portals take responsibility for users' defamatory replies for those reasons.

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Adaptive 1-D Transforms Order Selection Methods for Performance Improvement of SA-DCT (SA-DCT 성능 향상을 위한 적응적 1차원 변환 순서선택방법)

  • Song, Joon-Ho;Moon, Joo-Hee;Chung, Jae-Won
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.39 no.4
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    • pp.442-454
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    • 2002
  • It's noticed that the SA-DCT (Shape-Adaptive Discrete Cosine Transform) produces different 2-dimensional transform coefficients according as the first 1-dimensional transform is applied in horizontal or vertical direction for an arbitrarily shaped boundary block. Performing the first 1-dimensional transform in the direction, which has higher spatial correlation or smaller shifting distance, can compact the energy on the smaller number of AC coefficients around DC. This paper shows, experimentally, the compaction capability improvement by choosing the first 1-dimensional transform direction with higher spatial correlation or smaller shifting distance. Two adaptive selection methods are proposed to decide efficiently the spatial direction with higher correlation in a boundary block. One is based on the gradients between DC coefficients of neighboring and current blocks, and the other is based on the final coding efficiency that means the number of bits required for coding the block. Using the MPEG-4 video coder, the proposed method shows coding efficiency gain up to 10.87% compared to the conventional SA-DCT method.

A Study on Face Awareness with Free size using Multi-layer Neural Network (다층신경망을 이용한 임의의 크기를 가진 얼굴인식에 관한 연구)

  • Song, Hong-Bok;Seol, Ji-Hwan
    • Journal of the Korean Institute of Intelligent Systems
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    • v.15 no.2
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    • pp.149-162
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    • 2005
  • This paper suggest a way to detect a specific wanted figure in public places such as subway stations and banks by comparing color face images extracted from the real time CCTV with the face images of designated specific figures. Assuming that the characteristic of the surveillance camera allows the face information in screens to change arbitrarily and to contain information on numerous faces, the accurate detection of the face area was focused. To solve this problem, the normalization work using subsampling with $20{\times}20$ pixels on arbitrary face images, which is based on the Perceptron Neural Network model suggested by R. Rosenblatt, created the effect of recogning the whole face. The optimal linear filter and the histogram shaper technique were employed to minimize the outside interference such as lightings and light. The addition operation of the egg-shaped masks was added to the pre-treatment process to minimize unnecessary work. The images finished with the pre-treatment process were divided into three reception fields and the information on the specific location of eyes, nose, and mouths was determined through the neural network. Furthermore, the precision of results was improved by constructing the three single-set network system with different initial values in a row.

Changes of Optically Stimulated Luminescence Dosimeter Sensitivity with High Dose (고선량에 대한 광자극발광선량계의 방사선 민감도 변화 연구)

  • Han, Su Chul;Kim, Kum Bae;Choi, Sang Hyoun;Park, Seungwoo;Jung, Haijo;Ji, Young Hoon
    • Progress in Medical Physics
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    • v.27 no.2
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    • pp.98-104
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    • 2016
  • We investigated the effect of high dose on the sensitivity of optically stimulated luminance dosimeters (OSLDs) on Co-60 gamma rays and used a commercial OLSD (Landauer, Inc., Glenwood, IL). New OSLDs were chosen arbitrarily and were irradiated with 1 Gy repeatedly. We confirmed the change in the radiation sensitivity after repeated irradiation. The OSLD sensitivity increased up to 3% after irradiating for seven times and decreased continuously after the eighth time. It dropped by approximately 0.35 Gy per irradiation. Finally, after irradiating for 30 times, the OSLD sensitivity decreased by approximately 7%. When the OSLDs were irradiated 10 times with 1 Gy after their irradiation using a high dose of 15 Gy and 30 Gy, their sensitivity decreased by 6% and 12%, respectively, compared to that before high-dose irradiation. The change in the OSLD sensitivity with a high dose could be modeled by an exponential equation. We confirmed the radiation sensitivity variation caused by a high dose, and the irradiation history of dosimeters was considered to reuse OSLDs irradiated with a high dose.

Synthesis of Styrenated Phenol Alkoxylate from Styrenated Phenol with Ethylene Carbonate over KOH/La2O3 Catalyst (KOH/La2O3 촉매상에서 Styrenated Phenol과 Ethylene Carbonate의 반응으로부터 Styrenated Phenol Alkoxylate의 합성)

  • Lee, Seungmin;Son, Seokhwan;Jung, Sunghun;Kwak, Wonbong;Shin, Eun Ju;Ahn, Hogeun;Chung, Minchul
    • Applied Chemistry for Engineering
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    • v.29 no.1
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    • pp.62-66
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    • 2018
  • Styrenated phenol alkoxylates (SP-A) were prepared from styrenated phenol (SP) and ethylene oxide (EO) under a homogeneous base catalyst. However, to use EO that is difficult to handle, a high-pressure reaction device capable of controlling the reaction process should be used. Additionally, when a homogeneous base catalyst is used, a neutralization process is required to remove residual bases after the reaction, and it is also difficult to separate the catalyst and the product. Therefore, in this study, we report the results of SP-A prepared from the reaction of SP and EC using only heterogeneous base catalysts. The heterogeneous base catalyst was obtained by supporting KOH on $La_2O_3$ and calcintion. Using EC instead of EO, it was possible to produce SP-A under the atmospheric rather than high-pressure reaction condition. Average molecular weights of synthesized SP-A varied greatly depending on reaction conditions. The average molecular weight of SP-A prepared using the $KOH/La_2O_3$ catalyst could be controlled arbitrarily by controlling the reaction temperature and added catalyst and EC amounts.

The Estimation of Tree Form Index for Major Canopy Species in the Natural Deciduous Forest (천연활엽수림(天然闊葉樹林) 주요(主要) 상층(上層) 임관(林冠) 수종(樹種)의 임목(林木) 형질(形質) 지수(指數) 추정(推定))

  • Kim, Ji Hong;Yang, Hee Moon
    • Journal of Forest and Environmental Science
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    • v.15 no.1
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    • pp.1-9
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    • 1999
  • Noticing the intrinsic growing habit variations of hardwood species which is indigenous to the natural deciduous forest, eighteen tree species in overstory were selected for comparative evaluating tree form so as to develop the tree form index (TFI). Selected six tree form attributes were arbitrarily divided four assessment criteria and given appropriate scores. Eighteen tree species were undergone corresponding scores, converted to percentage base, and estimated TFI by the summation of the six scoring values. Cluster analysis was carried out to review which attribute would have been shared among species based on dissimilarity of scores for each pair of species in $18{\times}6$ data matrix. The result showed that Populus davidiana had the highest TFI value of 80.8 and Carpinus cordata had the lowest TFI va1ue of 46.3. The species which received more than 70 of TFI are Populus davidiana, Betula costata, Fraxinus mandshurica, and Ulmus davidiana var. japonica, characterized by the advantage of straight and longer bole, the narrower crown, and the thinner branch. On the contrary, such species as Sorbus alnifolia. Prunus sargentii, Acer mandshuricum, Juglans mandshurica, and Carpinus cordata received less than 60 of TFI, characterized by the disadvantage of the crooked or forked stem. the wider or deeper crown. and the thicker branch. In the dendrogram produced by cluster analysis, arbitrary value of Euclidean distance 10 divided eighteen species into four distinctive groups. and the typical characteristics of each group were discussed.

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Research on the status of children's dental health following mothers' dental health knowledge and behavior (일부 어머니의 구강보건지식 및 행태에 따른 자녀의 구강건강상태에 관한 연구)

  • Kwun, Hyeon-Sook;Lee, Eun-Gyeong;Cho, Gab-Suk
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.4
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    • pp.670-684
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    • 2009
  • Objectives : This research analyzed the general characteristics that closely affect the children's dental health and the mothers' dental health knowledge and dental health behavior to identify the correlation of the latter with the status of children's dental health in order to provide the basic data for the development of dental health business that would target children. Methods : Research subjects were selected arbitrarily from three nursery schools located in Busan metropolitan city, targeting 186 children between the ages of four to six and their mothers. The children were subjected to dental Inspection, and the status of their dental caries was studied whereas the mothers were subjected to the surveys on the general characteristics, dental health knowledge and dental health behavior. Results : 1. Level of mothers' dental health knowledge is higher when the mothers' educational level is higher(p=0.02) and when the household's monthly income is higher(p=0.009). 2. When the level of mothers' dental health knowledge is higher, children tended to brush their toothbrushing using proper method(p=0.025). Moreover, when the level of mothers' dental health knowledge is higher, they tended to take their children to dental clinic mostly for preventive measures than for treating cavity(p=0.023). 3. When the level of mothers' dental health knowledge is higher, children's dmft index was significantly low(p=0.02). When the mothers use fluoride-containing toothpaste, children's children' dmft index was even lower(p=0.02). 4. As the children tended to brush their teeth more often, dmft index was lower(p=0.003). When the reason that the children visited dental clinic was more to prevent, than to treat cavity, dmft index was even lower(p=0.000) Conclusions : When the above mentioned results are summarized, it is possible to know that the mothers' dental health knowledge and dental health behavior significantly affect children's dental health. Accordingly, it is necessary to develop maternal and child dental health program that factors in both the mothers and children in order to prevent children's dental caries and to increase their dental health, and continued care is required to discover and treat dental caries early on.

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Characterization of Cucumver mosaic virus Isolated from Hydrangea macrophylla for. otaksa (Sieb. et Zucc) Wils. (수국에서 분리한 Cucumber mosaic virus의 특성)

  • 방주희;박선정;이금희;최장경;이상용
    • Research in Plant Disease
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    • v.7 no.1
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    • pp.1-7
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    • 2001
  • An isolate of Cucumber mosaic cucumovirus(CMV) was isolated from Hydrangea macrophylla for. otaksa(Sieb. et Zucc. ) Wils. showing mosaic symptoms, and designated as Hm-CMV. Hm-CMV was characterized by the tests of host range, physical properties, serological properties, RNA and coat protein compositions, and reverse transcription and polymerase chain reaction (RT-PCR) analysis. Twelve species in 4 families were used in the host range test of Hm-CMV and could be differentiated from Y-CMV used as a control CMV by the ringspot and line pattern on inoculated leaves of several tobacco plants. Thevirus produced local lesions on inoculated leaves of Chenopodium amarticolor, C. quinoa and Vigna unguiculata. The physical properties of the virus were as follows; thermal inactivation point(TIP) was 60$\^{C}$, dilution end point (DEP) was 10$\^$-3/, and longevity in vitro (LIP) was 3∼4 days. Hm-CMV was serologically identical to Y-CMV. SDS-polyaciylamide gel electrophoresis(SDS-PAGE) showed one major protein band of about 28 kDa. In RNA or dsRNA analysis, Hm-CMV consisted of four RNA or dsRNA species, but satellite RNA was not detected. In RT-PCR using CMV-common primer and CMV subgroup I-specific primer, bothe amplified expected size of about 490 bp and 200 bp DNA fragments from Hm-CMV, respectively. Restriction enzyme analysis of the 490 bp RT-PCR products using EcoR I and Msp I showed that Hm-CMV belonged to CMV subgroup I. However, Hm-CMV could be differentiated from other CMV subgroup I isolates by RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR).

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Improvement Effect of Sprout of Coix lacryma-jobi var. mayuen Stapf Water Extract on DSS-Induced Ulcerative Colitis in Mice. (DSS로 궤양성 대장염 유발된 동물모델에서 의이아(薏苡芽) 열수 추출물의 개선 효과)

  • Kim, Min Ju;Shin, Mi-Rae;Lee, Jin A;Park, Soon-Ae;Park, Hae-Jin;Lee, Jeong Hoon;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.35 no.6
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    • pp.21-28
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    • 2020
  • Objectives : The objective of this study was to investigate the improvement effect of Sprout of Coix lacryma-jobi var. mayuen Stapf water extract (SC) on the dextran sulfate sodium (DSS)-induced ulcerative colitis mice. Methods : The antioxidant activity of SC was measured through total polyphenol and total flavonoid content in vitro. The experiment was conducted with seven-week-old male Balb/c mice. After 1 week adaptation, acute colitis was induced by oral administration of 5% DSS dissolved in drinking water, for 7 days. And normal mice received drinking water without DSS throughout the entire experimental period. For each experiment, the mice were divided into 4 groups and 24 colitis mice were arbitrarily allocated into 3 groups (n = 8/group); Normal group, Control group, SC 100 mg/kg treated group (SCL), SC 200 mg/kg treated group (SCH). Serum and colon tissues were collected after one weeks of drug administration. Results : ROS levels, ONOO- levels, AST, and ALT in serum were decreased in SC treated groups compared to the control group. Western blotting measurements of Nrf2, HO-1, SOD, catalase, GPx-1/2, IL-4, IL-10, and Bcl2 showed that the SC treated groups was increased compared to the Control group. Also, western blot measurements of NF-κBp65, p-IκBα, COX-2, iNOS, TNF-α, IL-1β, Bax, and Caspase-3 showed that the SC treated groups was reduced compared to the Control group. Conclusion : Taken together, these results suggest that SC treatment can attenuate the DSS-induced colitis though inhibiting NF-κB pathway and enhancing Nrf2 pathway. Therefore, SC was the potential to be used as a natural therapeutic drug.