• 제목/요약/키워드: arabidopsis

검색결과 822건 처리시간 0.035초

The Structure-Based Three-Dimensional Pharmacophore Models for Arabidopsis thaliana HPPD inhibitors as Herbicide

  • Cho, Jae Eun;Kim, Jun Tae;Kim, Eunae;Ko, Young Kwan;Kang, Nam Sook
    • Bulletin of the Korean Chemical Society
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    • 제34권10호
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    • pp.2909-2914
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    • 2013
  • p-Hydroxyphenylpyruvate dioxygenase (HPPD) is a potent herbicide target that is in current use. In this study, we developed a predictive pharmacophore model that uses known HPPD inhibitors based on a theoretically constructed HPPD homology model. The pharmacophore model derived from the three-dimensional (3D) structure of a target protein provides helpful information for analyzing protein-ligand interactions, leading to further improvement of the ligand binding affinity.

Cloning, Characterization, and Functional Analysis of Maize DEHYDRIN2

  • Paek, Nam-Chon;Jung, Hun-Ki
    • 한국작물학회지
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    • 제47권2호
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    • pp.116-122
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    • 2002
  • Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK$_3$ LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.

Extracellular Polymeric Substances of Pseudomonas chlororaphis O6 Induce Systemic Drought Tolerance in Plants

  • Cho, Song Mi;Anderson, Anne J.;Kim, Young Cheol
    • 식물병연구
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    • 제24권3호
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    • pp.242-247
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    • 2018
  • Pseudomonas chlororaphis O6 induces systemic tolerance in plants against drought stress. A volatile, 2R, 3R-butanediol, produced by the bacterium causes partial stomatal closure, thus, limiting water loss from the plant. In this study, we report that applications of extracellular polymeric substances (EPS) from P. chlororaphis O6 to epidermal peels of leaves of Arabidopsis thaliana also reduce the size of stomatal openings. Growth of A. thaliana seedlings with applications of the EPS from P. chlororaphis O6 reduced the extent of wilting when water was withheld from the plants. Fluorescence measurements showed photosystem II was protected in the A. thaliana leaves in the water stressed EPS-exposed plants. These findings indicate that P. chlororaphis O6 has redundancy in traits associated with induction of mechanisms to limit water stress in plants.

Overview of Brassica Genome Structure based on Comparative Genomics with Arabidopsis: Sequencing 629 comparative-tile BACs and their utility for physical mapping of B. rapa genome

  • Yang Tae-Jin;Kwon Soo-Jin;Kim Jung-Sun;Lim Ki-Byung;Kim Jin-A;Jin Mi-Na;Park Jee-Young;Choi Beom-Soon;Lim Myung-Ho;Kim Ho-Il;Lim Yong-Pyo;Kim Hyung-Tae;Park Beom-Seok
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2006년도 한국육종학회 공동심포지엄 및 학술대회
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    • pp.6-6
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    • 2006
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