• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1060-1066
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• 2005

Under environmental stress, such as strong irradiance or nitrogen deficiency, unicellular green algae of the genus Haematococcus accumulate secondary carotenoids, i.e. astaxanthin, in the cytosol. The induction and regulation of astaxanthin biosynthesis in microalgae has recently received considerable attention owing to the increasing use of secondary carotenoids as a source of pigmentation for fish aquacultures, and as a potential drug in cancer prevention as a free-radical quencher. Accordingly, this study generated expressed sequence tags (ESTs) from a library constructed from astaxanthin-induced Haematococcus pluvialis. Partial sequences were obtained from the 5' ends of 1,858 individual cDNAs, and then grouped into 1,025 non-overlapping sequences, among which 708 sequences were singletons, while the remainder fell into 317 clusters. Approximately $63\%$ of the EST sequences showed similarity to previously described sequences in public databases. H. pluvialis was found to consist of a relatively high percentage of genes involved in genetic information processing ($15\%$) and metabolism ($11\%$), whereas a relatively low percentage of sequences was involved in the signal transduction ($3\%$), structure ($2\%$), and environmental information process ($3\%$). In addition, a relatively large fraction of H. pluvialis sequences was classified as genes involved in photosynthesis ($9\%$) and cellular process ($9\%$). Based on this EST analysis, the full-length cDNA sequence for superoxide dismutase (SOD) of H. pluvialis was cloned, and the expression of this gene was investigated. The abundance of SOD changed substantially in response to different culture conditions, indicating the possible regulation of this gene in H. pluvialis.

• Journal of Aquaculture
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• v.12 no.2
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• pp.101-105
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• 1999

Predater, tarpon (Megalops atlanticus) has a specific preferring preys in natural environments. To investigate this, young tarpon were captured and their stomach contents were weighed and sorted based on taxonomic groups. Average standard length and body weight for tarpon captured were 11.49 cm and 34.67 g, respectively. The average weight of the stomach contents in the tarpon was 0.83 g. The status of digestion extent of the consumed preys was 2.37 which indicated that preys were relatively well digested. The main preys in tarpon stomach were other fishes (mosquito fish and sheepshead minnow) and shrimp. Frequency occurrence of fishes in tarpon stomach was highest (P＜0.05). Also, frequency occurrence of shrimp was higher than that of fly (P＜0.05). Mean individual number of fish found in tarpon stomach was 2.66 per tarpon and it was higher than that of shrimp or fly (P<0.05). There was linear regression between the total weight of the consumed preys and the standard length of tarpon; Y (Total weight of preys) = 0.3151 $\times$ (Standard length of tarpon) - 2.7123, $R^2$=0.6140. Also, there was the strong linear regression between the total weight of the consumed preys and weight of the tarpon; Y (Total weight of preys) = 0.0299 $\times$ (Body weight of tarpon) - 0.0774, $R^2$=0.7882. These results indicated that the main preys for young tarpon in their natural environments were small fish composed of mosquito fish and sheepshead minnow and shrimp. And there was the stronger correlation between the total weight of preys and the body weight of tarpon than that between the total weight of preys and the standard length of tarpon.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.677-683
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• 2017

The Korean aquaculture industry was generally began in the 1970s and has gradually increased. Now, the number of households is about 7,068 and the scale of an aquaculture farm is about 248,014 ha; the value of all production in the industry is estimated to be about 1602.2 billion won. The aquaculture industry is very valuable and important for future food resources. However, the aquaculture industry was damaged by several marine ecological disturbance causing organisms. The Ascdiella aspersa colonized on the shell of scallop and then scallops were detached from rope. The patterns of damage in the aquacultures were observed in Tongyeong (oyster), Geojedo (oyster), and Gangneung (sea squirt) in June, 2017, as well as in Tongyeong (oyster) in November, 2017 by SCUBA divers. The species Halichondria bowerbanki, Bugula neritina, Mytilus galloprovincialis, Balanus amphitrite, Ascidiella aspersa, Ciona intestinalis, Didemnum sp, Styela plicata in Tongyeong, M. galloprovincialis, A. aspersa, C. intestinalis, D. vexillum, S. plicata in Geojedo, and M. galloprovincialis in Gangneung were all usually found in their farms. The marine ecological disturbance causing organisms gave rise to a reduced number of aquaculture products.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.462-472
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• 2023

We investigated the physio-chemical and geochemical parameters in the spraying shellfish aquacultures (Yeoja and Gangjin Bay) to establish the systematic strategy for effective environmental management. Spatial variation of each parameter showed partially significant difference (P<0.05) between Yeoja and Ganjin Bay, inferring the discriminative progress (i.e., accumulation and degradation) of the autochthonous organic matter within the aquaculture environments. We additionally integrated various properties (e.g., water/sediment quality, natural hazard, and biological health) which may affect the biological growth within the aquaculture habitats based on the biogeochemical cycles related to environmental components and aquaculture species. We used a screening approach (i.e., one out-all out; OOAO) which can permit the assessment of the health levels of aquaculture species, the scoring for other parameters (seawater, sediment, and natural hazard) as three levels (excellent, moderate and poor) depending on the complex interactive properties occurring in the aquaculture environments. Actual, discriminative scores obtained via our case studies may confirm that these stepwise processes are effectively evaluated for optimal health conditions within the aquaculture habitats. Thus, this approach may provide valuable insights for effective environmental management and sustainable growth of aquaculture operation.

• Journal of Aquaculture
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• v.8 no.4
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• pp.271-283
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• 1995

Blue tilapia, Oreochromis aureus (Steindachner) swim-up fry were fed a ration containing 0, 1, 10, or 60 ppm $17\alpha-Methyltestosterone$ (MT) for 30 days (sex reversal period). Fish that had been fed 0 ppm MT-treated feed during the sex reversal period were subsequently fed rations containing either 0 ppm MT, 10 ppm MT, or 60 ppm MT for the next 39 days (post sex reversal period) : fish that had been fed 60 ppm MT-treated feed during the sex reversal period were subsequently fed rations containing either 0 ppm MT, 10 ppm MT, or 60 ppm MT during the post sex reversal period. One group was fed 1 ppm MT-treated feed, while another group was fed 10 ppm MT-treated feed during both periods. The following growing season (10 months later), the residual effects of MT on weight gain, body composition, and gonosomatic indices (GSI) were evaluated. MT had no residual effect on growth of either sex. Groups that ate MT-treated feed were significantly (P<0.05) larger than those that received no MT during the sex reversal period, because females in the latter group lowered average weight gain. MT had a positive residual effect on body fat content in males, but had a negative effect on body moisture content. MT had a positive residual effect on body moisture content in females. MT had a significant negative effect on male and female GSI at the end of the post sex reversal period. MT consumption during the sex reversal period had a significant negative residual effect on male GSI. MT fed after sexual development (post sex reversal period) did not have a residual effect on male or female CSI.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.192-200
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• 1998

This study was carried out to evaluate the effect of feeding higher protein feeds with lesser amount, but feeding the constant total protein input for all treatments, on water quality and nitrite toxicity in channel catfish ponds. There was no significant difference in survival rate among treatments $(P>0.05)$. Specific growth rate (SGR) for Treatment 1$(28\%\;protein\;and\;100\%\;of\;satiation)$ was significantly higher $(P>0.05)$ than for Treatment 3$(36\%\;protein\;and\;87.5\%\;of\;satiation)$, but not significantly higher than for Treatment 2 $(32\%\;protein\;and\;77.8\%\;of\;satiation)$ at constant digestible energy (DE), 3.08kcal/g (treatments 1, 2 and 3). At constant DE/P (treatments 4, 2 and 5), no significant difference in SGR was observed among treatments. Feed conversion ratio (FCR) slightly improved or improved as dietary protein level increased from $28\%$ to $32\%$ and feed allowance decreased by $12.5\%$, but did not improve as dietary protein level increased from $32\%$ to $36\%$ and feed allowance decreased by $22.2\%$, at constant DE and constant DE/P. There was no significant difference in water quality variables, such as total ammonia nitrogen (TAN), nitrite, chlorophyll a, soluble phosphorous concentrations among treatments, but significant difference in water quality variables over time as amount of feed fed increased $(P<0.0001)$. There was a trend toward increase in TAN and nitrite over time. A strong linear regression was observed between mean total ammonia nitrogen and nitrite for all treatments Y (Nitrite) =$0.04\times (TAN)+0.01$, $R_2=0.89$. Methemoglobin percent in the blood of catifish was not significantly different among treatments. And its mean value was $7.5\%$, which was relatively low, so that it was not serious problem in catfish production pond under these experiment conditions. There was the stronger linear regression between the percentage of Methemoglobin and the molar ratio of nitrite to chloride rather than nitrite alone: $Y\;(Methemoglobin\;\%)\;=\;58.45\;\times\;(NO^{2-}/Cl^-)\;+\;0.41,\;R^2=0.60$. These results indicate that deterioration of water quality has no strong impact on poor weight gain for $36\%$ dietary protein in this study.