• Title/Summary/Keyword: applied research

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Study on the quench protective circuit for inductively coupled superconducting magnet systems (자기적으로 결합된 초전도 마그네트의 퀜치보호에 대한 연구)

  • Ko, Rock-Kil;Bae, Joon-Han;Sim, Ki-Deok;Kwon, Young-Kil;Ryu, Kang-Sik
    • Proceedings of the KIEE Conference
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    • 2001.04a
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    • pp.41-43
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    • 2001
  • We studied on effective quench protection method to prevent damage from unexpected quench of the inductively coupled superconducting magnet systems for magnetic resonance imaging. And we suggested quench protection circuit that is combined with several protection techniques. This circuit has the capacity to maintain the symmetric nature of the magnetic field and the active shielding effect and to protect shim coils during a quench.

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Functional Analysis of the Stress-Inducible Soybean Calmodulin Isoform-4 (GmCaM-4) Promoter in Transgenic Tobacco Plants

  • Park, Hyeong Cheol;Kim, Man Lyang;Kang, Yun Hwan;Jeong, Jae Cheol;Cheong, Mi Sun;Choi, Wonkyun;Lee, Sang Yeol;Cho, Moo Je;Kim, Min Chul;Chung, Woo Sik;Yun, Dae-Jin
    • Molecules and Cells
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    • v.27 no.4
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    • pp.475-480
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    • 2009
  • The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expression systems.

In vitro Cytotoxicity of Sambutoxin

  • Kim, Jin-Cheol;Park, Jeng-Bae;Kim, Gye-Won;Kim, Won-Bae;Lee, Yin-Won
    • Applied Biological Chemistry
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    • v.41 no.4
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    • pp.273-274
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    • 1998
  • In vitro cytotoxicity of sambutoxin was measured by using various human and murine tumor cells and $IC_{50}$ values of sambutoxin ranged from 46.2 to 1,425.6 ng/ml.

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