• Parasites, Hosts and Diseases
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• 제44권3호
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• pp.233-237
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• 2006

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

• 한국잠사곤충학회지
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• 제39권2호
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• pp.161-168
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• 1997

To clarify the effect of anti-juvenile hormone analogue (AJH) on the larval ecdysis by feeding at early stage of the 4th instar, the total amount of protein and activity of chitinolytic enzymes in the integument of Bombyx mori were analyzed, PAGE pattern of the protein was observed and the morphological changes of integument during molting period were also observed and the morphological changes of integument during molting period were also observed by means of TEM. The total amount of protein was greatly increased in premolting, then reached maximum level just before ecdysis, and rapidly decreased after the larval ecdysis in the control, while in the AJH treatment, increased 12 hr later than the control and its maximum was only 82.6% of the control. Two specific proteins, which were presumed as the protein originated from endocuticle, also appeared 12 hr later than the control and were maintained to 132 hr after AJH treatment from the aspects of the Native- and SDS-PAGE patterns, although those of the control disappeared instantly after ecdysis. Chitinase and $\beta$-N-acetylglucosaminidase activities were also suppressed and delayed by AJH treatment. Furthermore, it was observed that the apolysis took place 12 hr later than the control but new epicuticle was not formed at least until 132 hr after AJH treatment. From these results, it is suggested that the larval molting process of silkworm develops 12 hr later than the control but new epicuticle was not formed at least until 132 hr after AJH treatment. From these results, it is suggested that the larval molting process of silkworm develops 12 hr later than the control by AJH treatment but no further processing takes place just after apolysis.

• Applied Microscopy
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• 제24권4호
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• pp.98-106
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• 1994

The corneal formation of compound eye of Pieris rapae L., which was mostly made during pupal stage, was morphologically investigated with light microscope, scanning electron microscope and transmission electron microscope. The regeneration of the microvilli were found on the surface membranes of corneagen cells and retinular pigment cells of preommatidium after apolysis pupal cuticle. The microvilli were finally differentiated to corneal nipples of the ommatidium. The corneal cuticle was generated on the superficial layer of the preommatidium from corneagen cells and retinular pigment cells. The corneal process was also formed under the cuticular layer from the corneagen cells. The pore canal was appeared within the cuticular layer and connected with the retinular pigment cell as if the root of interommatidial hair was connected. The interommatidial hair was projected randomly among the ommatidial facets and cornal nipple was arrayed regular on the ommatidial facets. The cornea was convex lens and the refracting power by its convex shape was 4 diopter.