• 한국식품영양학회지
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• 제7권2호
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• pp.128-136
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• 1994

Mackerel muscle protein hydrolysates, which were prepared from defatted mackerel meal by proteases such as complex enzyme, alcalase, bromelain, pancrease, pepsin, w-chymotrypsin, trypsin and papain, were tested for the antioxidative action against linoleic acid. Among proteases tested, the hydrolysates obtained from the treatment of complex enzyme, bromelain and alcalase showed higher antioxidative effects. Also, the hydrolysates showed the synergistic effects with o-tocopherol and the inhibitory effects for peroxidation of metal ions(Fe3+, Cua+) From the profiles of fractionation of the hydrolysates with Bio-gel P-2 column, the most active fractions, part I(complex enzyme-derived) and part e(bromelain-derived), had below MW 1,400 and the antioxidative effects were closely related to the binding capacity with metal ion(Cua+). Amno acid composition of the part I was abundant in histidine, arginine, phenylalanine and lysine, and the part e was abundant in lysine, glutamic acid and leucine.

• Biomolecules & Therapeutics
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• 제8권4호
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• pp.349-353
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• 2000

Mefenamic acid has been widely used as clinical drug for anti-inflammatory and analgesic. This drug was known to non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, ibuprofen and indomethacin. Although the drugs which comprise this group are of diverse chemical structures, they all share the antipyretic, analgesic and anti-inflammatory actions which are characteristic of aspirin. Action of this drugs is caused by inhibitory effect of biosynthesis of prostaglandin that are synthesized from arachidonic acid via the endoperoxide biosynthesis pathway, the initial step of which is catalysed by cyclooxygenase. Mefenamic acid has more potent inhibitory action of prostaglandin biosynthesis than aspirin. Therefore, mefenamic acid is expected to have anticoagulant activity as aspirin-like drugs. This study was carried out to investigate the sinthesis of mefenamic acid derivatives from mefenamic acid and aromatic compound of antioxidant and its antioxidative and anticoagulant activities. Synthesis of mefenamic acid derivatives was conformed by conjugation as using esterification method. Biological activities was examined using effect of anticoagulant on bleeding time and effect of antioxidant by TBA method. As a result, SJ-202 showed strong antioxidative activity and anticoagulant activity among tested 4 compounds and exhibited similar activity to aspirin at anticoagulant activity.

• 대한가정학회지
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• 제24권1호
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• pp.43-51
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• 1986

In order to study antioxidative acton of garlic, alliin, scordinin, garlic oil, ethanol fraction and non-kaolin fraction which have been discovered from garlic until now were extracted and isolated, and each fraction was utilized as the experimental materials. Antioxidative action of each fraction was compared through the in vitro and in vivo experiments. Electron donatingability on $\alpha$, $\alpha$-diphenyl-$\beta$picrylhydra-$\chi$yl, the inhibitory effect of lipoperoxide formation by TBA and peroxide value were measured and analyzed. RESULTS : 1. When observed antioxidative ability by EDA value, ethanol fraction of garlic components showed the strongest reaction as 15.25. 2. In vitro experiment with TBA value, garlic oil, alliin and ethanol fracton showed distinctive effect on inhibitory effect of lipoperoxide formation. 3. Comparing with the inhibitory effect of lipoperoxide formation with TBA value in vivo, the ethanol fraction was the most effective in the blood or liver by intraperitoneal administration, whereas the ethanol fraction in the blood and non-kaolin fraction in the liver was most effective each other by orally administration. 4. In vitro experiment with peroxide value, garlic oil was distinctive effect on the inhivitory effect of lipoperoxide formation, which was a similar to the trend of TBA value in vitro. 5. Examining the induction time for the first period of lipoperoxide formation in vitro, garlix oil, ethanol fraction and alliin were effective, which was a similar to the trend of TBA value and peroxide value in vitro.

• 한국식품영양과학회지
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• 제24권2호
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• pp.299-304
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• 1995

Antioxidative effect of tea extracts from green tea(steamed, roasted), oolong tea and black tea was investigated. Antioxidative activities of the crude catechin fraction were the most effective in oolong tea which contained the highest level of (-)-epigallocatechin gallate(EGCg). The water-soluble fraction obtained from oolong tea exhibited binding with more ferrous than copper ions. It showed a synergistic effect when used with an antioxidant such as BHT(0.5mg) and $\alpha$-tocopherol(2mg). Especially, this synergistic effect was exhibited more with BHT than with $\alpha$-tocopherol. Also steamed green tea, roasted green tea and oolong tea showed remarkable free radical scavenging action. The SC50(the concentration of a water-soluble fraction which is required to scavenge 50% of 100$\mu$M 1, 1-diphenyl-2-picryl hydrazyl radicals) of steamed and roasted green tea, oolong tea and black tea extracts were 11.3$\mu\textrm{g}$/ml, 11.4$\mu\textrm{g}$/ml, 12.7 $\mu\textrm{g}$/ml and 14.9$\mu\textrm{g}$/ml, respectively. It is assumed that the antioxidative activity of tea extracts is due to inhibition of peroxidation free radical scavenging and binding action of ferrous ions by mainly tea polyphenol compounds.

• 한국식품영양과학회지
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• 제21권6호
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• pp.686-692
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• 1992

아미노산의 전하 특성에 따른 Maillard 반응생성물이 항산화성에 미치는 영향을 살펴보기 위하여 amino-carbonyl 반응 화합물과 시판 항산화제의 항산화작용을 비교하고 이들간의 상승작용을 검토하였다. 즉 fructose-amino acid(glycine, histidine, aspartic acid) 모델계를 사용하여 비분획성 melanoidin과 비투석성 melanoidin을 조제하여 이들 모델계의 melanoidin과 항산화제인 BHT, TBHQ, ascorbic acid, ${\alpha}$-tocopherol, lecithin을 각각 linoleic acid에 소정량 첨가하고 $50^{\circ}C$에서 일정기간 저장하여 linoleic acid의 산화 안정성을 비교 검토하였으며 또한 이들을 혼합 사용하였을 경우의 상승효과를 조사한 결과는 다음과 같다. Melanoidin의 항산화성은 비분획성보다 고분자 melanoidin인 비투석성의 경우가 전반적으로 항산화성이 큰 것으로 나타났다. 그리고 아미노산의 종류에 따른 항산화작용에 있어 비분획성의 경우는 glycine과 histidine이 산성아미노산인 aspartic acid를 사용한 경우보다 항산화성이 더 큰 것으로 나타났으녀, 비투석성의 경우는 아미노산의 종류에 따른 차이는 크지 않은 것으로 나타났다. 한편 melanoidin의 농도가 높을수록 항산화 효과는 크게 나타났다. Melanoidin과 항산화제의 항산화성의 비교에 있어서는 비분획성 melanoidin계는 합성 항산화제인 BHT, TBHQ, ascorbic acid보다는 항산화성이 떨어졌으나 천연 항산화제인 ${\alpha}$-tocopherol과 lecithin보다는 항산화성이 우수한 것으로 나타났다. 반면에 비투석성 melanoidin계에 있어서는 합성 항산화제의 항산화성과 거의 동일하게 인정되었으며, 천연 항산화제 보다는 항산화성이 매우 우수한 것으로 나타났다. 항산화제와의 상승효과는 천연 항산화제인 ${\alpha}$-tocopherol과 lecithin에 melanoidin 모델계 6종류를 각각 병용하여 사용한 결과 fructose-aspartic acid계 및 fructose-glycine계 비분획성 melanoidin을 제외한 4종류는 그 상승효과가 매우 큰 것으로 나타났다. 한편 합성 항산화제인 BHT, TBHQ, ascorbic acid와 melanoidin을 병용사용한 경우는 상승효과를 측정하기가 곤란하였는데, 이것은 합성 항산화제만으로도 강력한 항산화 효과를 나타내었기 때문이라 생각한다.

• 한국식품과학회지
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• 제14권2호
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• pp.130-135
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• 1982

인삼중에 함유되어 있는 항산화물질의 본태를 파악하기 위하여 인삼의 각종 용매(petroleum ether, chleroform, chleroform-methanol, methanol 및 methanol-water)추출물들이 혼합 불포화지방산 메틸에스테르(MEUFA)의 자동산화에 미치는 영향을 in vitro에서 검토한 결과는 다음과 같다. 1. 인삼의 각종 용매 추출물들은 모두 항산화작용을 나타내어 MEUFA의 자동산화에 의한 지질 과산화물의 형성을 억제하였다. 그 중에서도 클로로포름 및 클로로포름-메탄올(2 : 1, v/v)추출물들이 보다 강한 항산화작용을 나타내었다. 2. Olcotte 등의 oven test에 의한 무게의 증가는 MEUFA의 자동산화에 의한 과산화물의 형성에 기인함이 확인되었다. 3. 인삼의 각 추출물들은 MEUFA의 지질 과산화물형성의 유도기간을 연장시켰다. 4. 각 추출물들의 항산화작용에 있어서 Olcott 등의 oven test에 의한 무게의 측정 Lea법과 Rhodan 철법에 의한 POV 측정 등의 결과에서 추정되는 작용은 상호간 잘 일치되었다. 이상의 결과로부터 인삼중에는 항산화물질들이 인삼성분으로서 함유되어 있음이 in vitro에서 확인되었다.

• 생약학회지
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• 제29권1호
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• pp.28-34
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• 1998

It has been known that diseases related with aging or cancer result, at least in part, from free radicals, and antioxidants may reduce or prevent the abnormalities associated with free radical formation via its scavenging action. Thus, this study was carried out to investigate the antioxidative and antimicrobial activities of nut species for the purpose of developing antioxidant from natural products. Antioxidant activities of four kinds of nuts such as chestnut, groundnut, walnut and acorn were examined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The extracts from chestnut hull and acorn barnyard millet showed strong antioxidative activities among 10 samples tested. Because of their highest antioxidative activity among 10 samples, radical scavenging effects of 4 different extract compartments (Hexane, EtOAc, BuOH and $H_2O$ extracts) from chestnut hull and acorn barnyard millet, were further examined by DPPH method. EtOAc and BuOH extracts exhibited antioxidative activities similar to those of natural, tocopherol or synthetic antioxidants, BHA. These findings demonstrates that major fraction of the antioxidative activity of chestnut hull or acorn barnyard millet was the EtOAc and BuOH extract compartments. However, antimicrobial activities against food-related bacteria and yeasts was relatively weak.

• Toxicological Research
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• 제27권1호
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• pp.37-41
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• 2011

This study investigated the antioxidative action of Corni Fructus aqueous extract on kidneys of diabetic mice. The electron donating abilities of Corni Fructus aqueous extract and its antioxidant activities (XO, SOD, CAT, GST, eNOS) in kidneys of C57BL/6 or db/db mice were evaluated. For in vivo study, seven week-old male mice were divided into normal control group (NC, C57BL/6 mice), diabetic control group (DC, db/db mice) and Corni Fructus (500 mg/kg/day for 8 weeks) treated diabetic group (DCF, db/db mice). The electron donating abilities of Corni Fructus aqueous extract exhibited 7%, 24.4%, and 42.7% at concentrations of 100, 500, and $1000\;{\mu}g/ml$, respectively. The activity of XO in the DCF group was significantly lower than the DC group by 35% (p < 0.05). The SOD activity was significantly higher in the DCF group than the DC group by 26% (p < 0.05). The activities of CAT and GST were lowered in the DCF group than the DC group by 26% (p < 0.05) and 7.6%, respectively. The mRNA expression of eNOS in kidneys was lower in the DCF group than the DC group by 24%. These results indicate that Corni Fructus reduced oxidation stress as evidenced by the restoration of the enzymatic antioxidative defense system in renal tissues of db/db mice. It is suggested that these antioxidative actions of Corni Fructus on renal tissues in db/db mice could contribute to its renoprotective effects on diabetic nephropathy.

• 한국식품영양과학회지
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• 제30권3호
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• pp.547-551
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• 2001

The comparative activities of acetone, ethanol, and aqueous fractions extracted from fruit powder of Cudrania tricuspidata by different temperature were tested by in vitro experimental models; peroxidation of linoleic acid and autooxidation of rat hepatic and renal microsomes by using thiobarbituric acid (TBA) for assay of free malondialdehyde production, and scavenging activities of free radicals by DPPH (α, α'-diphenyl-β-picrylhydrazyl). In DPPH method, acetone fraction extracted at 30℃ showed the highest free radical scavenging activities and acetone fractions extracted at 30℃ and 60℃ and ethanol fraction extracted at 30℃ showed stronger than BHT (butylated hydroxitoluene) although used ten-fold lower concentrations. In thiocyanate method used linoleic acid an inhibitory effects of all fractions showed higher than control treatment. TBA method used linoleic acid showed the highest antioxidative activity in acetone fraction extracted at 30℃ and 60℃. an inhibition activity against lipid peroxidation in hepatic microsomes of rats showed the highest at acetone faction extracted at acetone fraction among extracted fractions was shown to be the most potent antioxidative properties and this action was more potent in fractions extracted at 30℃ than those extracted at 60℃.

• 대한가정학회지
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• 제24권1호
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• pp.53-58
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• 1986

In order to study the antioxidative action of the effectient garlic components, peroxidase and superoxide dismutase activity were compared through the in vitro and in vivo experiments. RESULTS : 1. Observing the effects on peroxidase activity of efficient components in vitro, garlic oil, alliin and ethanol fraction showed effective, which was similar to the trend of TBA value, peroxide value and induction time for the first period of lipoperoxide formation in vitro. 2. In vivo experiment with peroxidase activity, the ethanol fraction and garlic oil were effective when intraperitoneally administered as well as orally administered. 3. Considering the superoxide disutase activity in vitro, the garlic oil, alliin and ethanol fraction were effective in efficient components. But non-kaolin fraction inhibited the activity on the contrary. 4. In terms of the efefcts on superoxide dismutase activity in vivo, alliin and garlic oil wee effective in intraperitoneal adminstraton and the ethanol fraction and alliin in oral administration.