• 한국조리학회지
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• 제21권3호
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• pp.259-269
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• 2015

본 연구에서는 중국산 대두의 천연 항산화성 소재로서의 활용 가능성에 대한 기초자료를 제공 하고자 페놀성 화합물 함량 및 항산화 활성을 측정하였다. 중국산 대두(Heinong 48)의 메탄올 추출물의 총 페놀 및 총 플라보노이드 함량은 각각 $9.91{\pm}0.57GAE\;mg/g$과 $4.15{\pm}0.39QE\;mg/g$이었다. 중국산 대두 추출물의 DPPH 라디칼 소거능은 농도 의존적으로 유의적으로 증가하였으며(p<0.05), $IC_{50}$는 $2.04{\pm}0.16mg/mL$이었다. 중국산 대두 추출물의 ABTS 라디칼 소거능은 농도 의존적으로 유의적으로 증가하였으며(p<0.05), $IC_{50}$는$3.31{\pm}0.31mg/mL$이었다. 이상의 결과로부터, 중국산 대두는 페놀성 화합물이 풍부하며, 항산화성 소재로서 활용 가능성이 높은 것으로 기대된다.

• 한국식품영양과학회지
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• 제45권1호
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• pp.149-154
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• 2016

현미와 홍국발효 콩 시료의 첨가량을 달리하여 제조한 식초의 미용건강기능성 소재(cosmeceuticals)로의 활용 가능성을 평가한 결과는 다음과 같다. DPPH와 ABTS 라디칼 소거능으로 측정한 항산화 활성은 현미 식초에 비해 홍국발효 콩이 첨가된 시료가 첨가량에 비례하여 최대 7배 이상 증가하였다(P<0.05). 특히 tyrosinase 저해 활성은 50% 첨가량의 시료에서 kojic acid의 저해 활성과 유사하게 나타나 미백 소재로서의 가능성을 보였으며, elastase 저해 활성 역시 첨가량에 비례하여 유의적으로 증가하였다(P<0.05). 현미 식초에 비해 홍국발효 콩을 1:1(w/w)의 비율로 첨가한 식초의 높은 생리활성은 시료에 함유된 총 페놀 함량, 특히 이소플라본 비배당체형의 genistein과 daidzein의 농도와 높은 상관관계가 성립되었다.

• 한국식품과학회지
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• 제52권4호
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• pp.363-368
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• 2020

단감연구소에서 출하 시기 조절을 위해 육성된 중생종 단감 신품종 홍추와 기존 재배품종 상서조생, 서촌조생, 부유 3품종의 잎 추출물을 제조하여 생리활성을 비교하였다. 그 결과, 홍추의 잎 추출물의 총페놀 함량, Vit. C 함량, DPPH 및 ABTS 라디칼 소거능, ADH 활성에 미치는 영향이 다른 추출물에 비하여 유의하게 높다는 결과를 얻을 수 있었다. ADH 활성도는 부유의 잎 추출물에서 다른 품종에 비해 비교적 높게 나타났다. 이상의 결과는 신품종 단감 홍추의 잎을 이용한 고품질 감잎차의 제조가 가능함을 시사한다.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.219-223
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• 2009

Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.335-342
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• 2017

Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer's demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with $1mg{\cdot}L^{-1}$ 6-benzylaminopurine (BAP) and $2mg{\cdot}L^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate ($AgNO_3$), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with $1mg{\cdot}L^{-1}$ BAP and $1mg{\cdot}L^{-1}$ gibberellic acid ($GA_3$), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with $1mg{\cdot}L^{-1}$ indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.

• 한국식품과학회지
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• 제41권3호
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• pp.345-349
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• 2009

더덕의 육질과 껍질의 부위별 세포벽 물질(CWM)로부터 uronic acid, 총 당, 비섬유성 중성당, 페놀성 화합물 함량 및 항산화 활성을 측정하였다. 더덕의 육질과 껍질의 uronic acid와 중성당의 비는 4, 6이었다. 총 당 함량은 육질과 껍질에서 각각 788.6, 824.9 ${mu}g/g$ CWM이었다. 주요 비섬유성 중성당은 galactose와 arabinose였다. 더덕 CWM으로부터 분석된 주요 페놀성 화합물은 vanillic acid, p-OH-benzaldehyde, vanillin, ferulic acid 및 8-O-4' diferulic acid 였다. 이들 중 p-OH-benzaldehyde, vanillin, 8-O-4' diferulic acid는 더덕에서 처음으로 동정된 것으로 인삼의 세포벽 페놀성 화합물 조성과 유사하였다. 더덕의 껍질 AIR에 함유된 8-O-4' diferulic acid의 함량은 56.1 ${mu}g/g$ CWM이었다. 더덕의 CWM로부터 얻은 ethanol 분획, ethanol 분획의 ethanol-NaOH 분획, ethanol 분획 후 잔사로부터 얻은 AIR 분획의 항산화 활성의 크기는 ethanol-NaOH 분획이 가장 높았으며, 다음으로 AIR 분획, ethanol 분획의 순으로 나타났다.

• 대한화장품학회지
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• 제37권2호
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• pp.171-176
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• 2011

N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin은 홍화씨에서 유래하는 특이적인 세로토닌유도체로 주로 식물이 병원균을 방어하기 위해서 만들어내는 hydroxycinnamic acid amides계 물질이다. 본 연구에서는 N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin이 지방전구세포의 지방분화에 미치는 영향을 oil-red O염색과 triglyceride 양 측정, GPDH 활성 측정 등을 통해 알아보았고, 그 결과 두 물질 모두 유의적으로 지방세포분화를 억제함을 관찰하였다. 효능 비교물질로 세로토닌을 처리했을 때 세로토닌 자체로는 지방분화에 유의미한 효과는 관찰되지 않았다. N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin은 또한 우수한 항산화능을 보여주었다. 이러한 결과를 통해, N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin이 지방세포분화억제를 통한 항비만 소재로서의 가능성을 확인하였다.

• Preventive Nutrition and Food Science
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• 제10권2호
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• pp.111-117
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• 2005

Radical scavenging activity of water and methanol extracts of mulberry juice and cake prepared from mulberry fruit (Morus spp.) was evaluated using three in vitro assay systems. Mulberry fruits were homogenized with $0.5\%$ trifluoroacetic acid (TFA) in distilled water, filtered with cheeze-cloth and centrifuged to yield mulberry juice and cake. Mulberry juice was evaporated and solubilized in $0.5\%$ TFA in distilled water or $0.5\%$ TFA in $80\%$ aqueous methanol, followed by filtration and evaporation to obtain water (WMJ) and methanol (MMJ) extracts of mulberry juice. Mulberrry cake also was extracted with the above same solvents, and thereby finally obtaining water (WMC) and methanol (MMC) extracts of mulberry cake. Among four extracts, the MMC showed the most potent radical scavenging activity against DPPH radical $(IC_{50}=167.45\;{\mu}g/mL)$, and superoxide $(IC_{50}=36.18\;{\mu}g/mL)$ and hydroxyl radicals $(IC_{50}=467.08\;{\mu}g/mL)$. The WMC also exhibited stronger radical scavenging activity than those of two other mulberry juice extract, WMJ and MMJ. Meanwhile, the MMJ exerted stronger three radical scavenging activity than the WMJ. Total phenolic content of the water and MeOH extracts from mulberry cake was higher than that of the water and MeOH extracts from mulberry juice. Thus, these results suggest that the extracts of mulberry cake with high dietary phenolics may be useful potential source of natural antioxidant as radical scavenger.

• 원예과학기술지
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• 제34권3호
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• pp.495-509
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• 2016

Irrigation applications (IA) and increased bud load (BL) are fundamental practices that are used to achieve optimum yields in grape production, while maintaining fruit quality parameters. Two different irrigation amounts (IA-I and IA-II) based on growth stages, in addition to a non-irrigated (rain-fed) control, along with two different BL applications [normal bud load based on traditional pruning practices (1BL) and double bud load (2BL)] were evaluated over a two-year experiment for their effects on the biochemical composition of the berries. Berries from the rain-fed vines had higher sugar levels, whereas no significant change was detected in organic acid levels. The increased bud load (2BL) treatment yielded less sugar in the berries compared to the 1BL control in both years. The total non-colored phenolic compounds (NPC) were greater in the irrigated (especially IA-I) and 2BL treatments than in those of the rain-fed and 1BL control. However, total anthocyanin was greater in the non-irrigated and 1BL control than in the irrigated and 2BL treatments. The antioxidant contents of the berries also varied according to the treatments and years. Our results implied that implementing a higher bud load along with the IA-I irrigation application, in which irrigation applications were 50 and 75% of the cumulative evaporation from the Class A pan during berry set to veraison and veraison to harvest growth stages, respectively, can help in obtaining greater yields in high-plateau viticulture. Thus, if more buds are left on the vines, along with sufficient irrigation and rainfall, yield may increase while maintaining or increasing the biochemical composition of the berries.

• 한국식품조리과학회지
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• 제25권4호
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• pp.406-414
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• 2009

This study was conducted to investigate the functional composition and antioxidant activity of Cirsium and Carduus genera based on different parts. Leaves of Cirsium setidens Nakai contained 23.66% protein and seeds of Carduus crispus L contained 25.30% lipid. Extraction yields of Cirsium and Carduus genera were higher in leaves than in any other parts of the plants. Total phenolics and total flavonoid content were abundant in extracts of leaves, steam and root of C. japonicum var. ussuriense, and the flower extract of C. setidens Nakai. Silymarin was not found in extracts of Cirsium and Carduus genera. Acacetin was identified in leaf or flower extracts of C. setidens Nakai, or in leaf and steam extracts of C. pendulum Fisch ex DC. Apigenin was identified in the flower extracts of Cirsium and Carduus genera and constituted 7.16 mg/g in C. japonicum var. ussuriense. Cynarin was present at 5.55 mg/g in the seed extract of C. setidens Nakai, and narirutin represented 19.56 mg/g and, 4.18 mg/g of the seed extracts of C. pendulum Fisch ex DC and, Carduus crispus L, respectively. 2,2-Diphenyl-1-2-picrylhydrazyl (DPPH) radical scavenger activity was higher in flower extracts of C. setidens Nakai than in the other genera. Photochemiluminescence activity was 2.3 nmol in leaves and flower extracts of C. setidens Nakai and flower extract of Carduus crispus L. Flowers and seeds of C. setidens Nakai shoe potential as new functional materials.