• 한국축산식품학회지
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• 제41권1호
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• pp.110-121
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• 2021

Meat industries utilize plant material such as celery in cured meat products. Extraction of valuable bioactive compounds, nitrates and nitrites often involves processes that increase cost or lack sustainability. Thus, this study investigated the effect of ball-milled celery powders (CP) on the physicochemical, antioxidant, and antimicrobial properties along with curing efficiency in comminuted meat product. Pork sausages loaded with CPs with different average particle sizes: 265 ㎛ (T1), 68 ㎛ (T2) and 7 ㎛ (T3) were compared to those added without and with sodium nitrite (150 ppm). The a⁎ values were increased for sausages with larger particle size. The L⁎ values decreased for all CPs. Residual nitrite for all particle sizes increased in the earlier stages and decreased at the end of storage period. The curing efficiency also increased for larger size particles with an increase until day 9 followed by a gradual decrease. Superfine CP had a tendency to improve the antioxidant activities. The antimicrobial activity of CPs was not comparable with nitrite added sausages. The textural parameters remained unaffected by particle size. Thus, instead of extracts or juices, micronized CPs could be used to improve the antioxidant activities and curing efficiency of label friendly reformulated meat products.

• 한국식품영양학회지
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• 제35권5호
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• 한국응용과학기술학회지
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• 제40권6호
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• pp.1363-1372
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• 2023

This study is concerned with the optimization of the manufacturing process of a hot water extract containing antioxidant activity from Lycium barbarum, traditionally known to have various physiological activities. For the establishment of the optimization process, the central composite design of response surface methodology(RSM) was used. Thirteen extraction processes were performed by encoding the independent variables, extraction temperature (65.9℃-94.1℃) and extraction time (2.59 hr-5.41 hr). As a result of the experiment, the optimal manufacturing conditions for the extract were 340.0 mg/100 g of GAE at an extraction temperature of 94.1℃ and an extraction time of 5 hr. The maximum yield of flavonoids was 22.44 mg/100 g of HES at an extraction temperature of 94.1℃ and an extraction time of 4 hr. The conditions for producing the extract with the maximum antioxidant capacity (DPPH 92.12%) were 90℃ and 4.5 hr extraction time. Therefore, the optimal manufacturing process conditions for extracts containing total phenol content, flavonoid content, and DPPH radical scavenging activity, which are dependent variables, were extraction temperature of 90-95℃ and extraction time of 4 hr, which were not significantly different from the actual values. Therefore, Lycium barbarum extract rich in total phenol and flavonoid content related to antioxidant function is expected to be used as a functional food and cosmetic material.

• 한국미생물·생명공학회지
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• 제52권1호
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• pp.65-75
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• 2024

The growing demand for functional cosmetics, driven by modern individuals' quest for healthy and aesthetically pleasing skin, is being challenged by reports of side effects and toxicity associated with these products. This underscores the importance of exploring natural plant-based materials for functional cosmetics. This research focuses on the assessment of antioxidant and whitening properties of mixed extracts from Ipomoea nil, Arctostaphylos uva-ursi, and Angelica gigas Nakai, all of which have proven pharmacological benefits. The study evaluated the extracts' total polyphenol and flavonoid contents, ABTS and DPPH radical scavenging activities, SOD-like activity, and xanthine oxidase inhibition to determine their antioxidant capabilities. Moreover, the whitening potential was investigated through tyrosinase activity and melanin production assays, alongside a cytotoxicity evaluation via a cell viability test. The findings revealed that the extracts, IAA-1 to IAA-4, demonstrated both antioxidative and whitening capabilities without exhibiting cytotoxic effects. Notably, the extract IAA-4, processed through ultrasonic and ultrahigh pressure extraction, exhibited superior effectiveness. These results indicate that the cavitation formed during ultrasonic irradiation effectively destroys the plant cell wall by creating high pressure, and as a result, it is judged that useful components are easily extracted. As a result of the study, it was confirmed that the IAA-4 extract could be applied as a material for functional cosmetics.

• 한국자원식물학회지
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• 제22권4호
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• pp.323-329
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• 2009

기능성 옥수수 수염을 가지는 옥수수 계통을 선발하기 위해 다양한 자식계통과 F1을 대상으로 옥수수 수염의 항산화 성분과 항산화능을 측정하였다. 측정결과, 총 페놀 함량, 총 플라보노이드 함량의 경우 각각 980${\sim}$2,420 mg 100 $g^{-1}$, 1,532${\sim}$3,274 mg 100 $g^{-1}$의 범위를 나타내었다. 안토시아닌의 고유영역인 517 nm에서의 흡광도는 0.05에서 0.76까지의 범위를 보였다. 옥수수 수염의 항산화 성분과 DPPH, ABTS, FRAP assay에서 얻어진 항산화능간의 상관관계를 분석한 결과, ABTS와 FRAP assay에서 옥수수 수염의 항산화 활성과 페놀성물질 및 안토시아닌간의 상관계수가 유의하게 높았다. 옥수수 수염의 안토시아닌을 추출하는 용매가 산성이라는 점과 안토시아닌의 DPPH 용액과의 가시영역 파장에서의 간섭을 고려할 때, DPPH 보다는 ABTS assay나 FRAP assay가 더 적합한 항산화능 측정 방법이 될 것이다. 이들 중 특히 ABTS assay는 옥수수 수염의 주요한 기능성 성분인 안토시아닌과 플라보노이드 및 페놀성 물질과 모두 높은 상관성을 보여 옥수수 수염의 종합적인 항산화능을 평가하고 우수 계통을 선발하는데 적합한 측정 방법이 될 것으로 사료된다. 본 실험에서 안토시아닌 함량과 페놀 함량이 높고 ABTS assay와 FRAP assay에서 모두 높은 항산화능을 보여준 S15은 고 기능성 품종 육종 및 옥수수 수염을 이용한 기능성 소재 개발에 이용될 수 있을 것이다. 또한 옥수수 수염의 항산화능 측정에 대한 항산화 실험의 적합성 연구는 우수한 옥수수 수염을 선발하는데 도움이 될 것이다.

• 한국식품영양과학회지
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• 제39권12호
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• pp.1725-1730
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• 2010

프로폴리스를 기능성 식품 재료로 활용하기 위한 기초자료로 지역에 따른 일반성분, 총 페놀성 화합물 함량 및 항산화 활성을 조사하였다. 일반성분 중 조지방이 가장 많이 함유되어 있었고, 조섬유가 가장 적게 함유되어 있었다. 총 페놀성 화합물은 거창산 에탄올과 물 추출물, 제주산 에탄올과 물 추출물에서 각각 184.17, 316.19, 204.33 및 47.83 mg/g으로 다른 추출물에 비해 거창산 물 추출물에서 총 페놀성 화합물이 가장 높았다. 프로폴리스 추출물을 이용하여 라디칼 소거활성, 환원력 및 FTC 방법을 이용하여 여러 가지 항산화 활성을 측정한 결과 모든 추출물에서 농도의존적인 경향을 보였으며, 거창산 물 추출물에서 가장 높은 항산화 활성을 보였다. 따라서 거창산 프로폴리스 물 추출물은 높은 항산화 활성을 바탕으로 기능성식품 소재로 활용할 가능성이 매우 높을 것으로 판단된다.

• 한국식품위생안전성학회지
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• 제36권1호
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• pp.86-92
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• 2021

식용곤충인 갈색거저리 유충을 기능성 소재로 활용하기 위하여 갈색거저리 유충 추출물의 산화방지 성분 및 활성을 확인하였다. 동결건조한 갈색거저리 유충 분말의 일반 성분은 수분함량 0.80 ± 0.06%, 조회분 3.29 ± 0.03%, 조단백질 54.07 ± 0.24%, 조지방 36.78 ± 0.66%이었다. 갈색거저리 유충 분말의 ethanol 농도별(95%, 70%, 50%, 물) 추출물의 총 phenolic compounds 함량은 50% ethanol 추출물에서 459.23 ± 1.05 mg/100 g으로 가장 높았으며, 총 flavonoid compounds 함량은 물 추출물에서 19.86 ± 0.69 mg/100 g으로 가장 높았다. 갈색거저리 유충 분말 추출물의 DPPH radical 소거 활성은 70% ethanol 추출물에서 82.60 ± 0.00%로 가장 높았으며, 물, 50% ethanol, 95% ethanol 추출물 순으로 높았다. ABTS radical 소거 활성은 50% ethanol 및 물 추출물에서 각각 97.57 ± 0.16%와 95.33 ± 0.41%로 높게 나타났으며, 유의적 차이는 없었다. 환원력의 경우 물 추출물에서 유의적으로 가장 높았으며, 50% ethanol, 70% ethanol, 95% ethanol 추출물 순이었다. 본 연구결과 갈색거저리 유충의 산화방지 성분의 함량이 많고 산화방지 활성을 우수함을 확인하였으며, 기능성 소재로서 활용할 가치가 있는 것으로 나타났다.

• 한국식품영양학회지
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• 제30권6호
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• pp.1133-1139
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• 2017

This study was conducted to investigate the antioxidant activities of extracts from various parts of the pine tree, which is known as a good source of functional food material. While ethanol extraction yields of pine bud and cone were higher than water extraction yields of pine bud and cone, water extraction yield of pine needle was higher than ethanol extraction yield of the pine needle. The content of polyphenols in the pine cone ethanol extract was 5 times higher than that in the pine bud and needle. Further, the content of flavonoids in the pine cone ethanol extract was 8 times higher than that in the pine bud and needle. DPPH radical scavenging effect of the pine cone ethanol extract was 3~5 times higher that of the pine bud and needle extract. Regardless of the extraction solvents, trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) of the pine cone were stronger than those of the other parts of the pine tree. Taken together, it can be expected that the pine cone can be practically used as an antioxidant substance in food and beauty industries.

• 미생물학회지
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• 제37권3호
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• pp.177-181
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• 2001

Bacillus licheniformis Bl을 이용하여 청국장발효를 거쳐 보존성이 높은 분말청국장을 제조하였다. 분말청국장의 다양한 생리활성 물질 중에서도 항산화물질의 존재여부와 그 효괴를 결정하였다. 분말청국장을 증류수로 추출하여, Sephadex G-75 Bel filtration chromatography 작업을 수행한 후, 390 nm에서 광범위한 분자량의 갈변물질을 검출 할 수 있었다. 1,1 diphenyl-2-picrylhydrazyl (DPPH)를 이용하여 갈변물질의 항산화도를 결정하였다. 분자량이 큰 갈변물질의 항산화도는 매우 약하였으나, 분자량이 작은 갈변물질에서는 10-20%의 항산화능이 결정되었다. 체내에서는 분자량이 작은 갈변물질의 흡수가 더 유리할 수 있다. 분말청국장의 항산화물질 추출에 ethanol을 이용했을때는 60.1%, methanol을 이용했을 때는 58.97%의 높은 항산화능을 보여주어, 증류수로 추출했을 때에 비해 휠씬 효 율적이었다. 또한 분말컹국장을 ethanol 5%이상 되게끔 녹였을 때 항산화도가 가장 컸다. 본 분말청국장에 존재하는 항산화물질은 항산화능이 우수하였는 데, 앞으로 안정성이 확보된다면, 식용류, 주류에 첨가하는 천연 항산화물질로 개발할 수 있을 것이다.

• 한국약용작물학회지
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• 제28권5호
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• pp.331-338
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• 2020

Background: The aim of this study was to analyze the total phenol and total flavonoid contents and antioxidant activity of steam-treated leaves and rhizomes of Polygonatum sibiricum Redoute. In addition, we aimed to confirm their potential use as cosmetic materials by investigating their anti-aging and skin-whitening activity. Methods and Results: The leaves and rhizomes of P. sibiricum were treated with steam at different temperatures for different durations, and the antioxidant activity (DPPH and ABTS radical scavenging activity) and total phenol and total flavonoid contents of each sample were tested. The steam temperature and treatment duration siginificantly affected the antioxidant activity and, total phenol and total flavonoid content of the leaves and rhizome of P. sibiricum. Treating the P. sibiricum samples with steam at 120℃ for 12 h, yielded higher total phenol and total flavonoid contents. Comparatively, the samples treated with steam at 120℃ for 12 to 24 h showed significantly higher antioxidant activity. Further, the steamed samples of P. sibiricum demonstrated collagenase and tyrosinase inhibition activity, which indicated their anti-aging and skin-whitening properties. The samples steamed at 120℃ for 12 h, exhibited higher collagenase and tyrosinase inhibition activity. Conclusions: Leaves and rhizomes of P. sibiricum steamed at 120℃ for 12 h, showed highest antioxidant activity and, total phenol and total flavonoid contents than all other samples. Our results indicate the potential of using P. sibiricum as a cosmetic material by confirming its excellent anti-aging and whitening activity.