• 한국식품영양과학회지
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• 제23권4호
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• pp.604-613
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• 1994

Hydrophilic brown pigments(HBPs) produced during fermentation and aging the domestic Meju and Doenjang were separated by dialysis and chromatography , and their antioxidant activity were measured . The chemical properties of HBP were determined by UV and IR spectrophotometry. HBPs contents were found to be 93.1 mg/g and 183.2mg/g in Meju fermentated for 30 days and 80 days , respectively. The ratio of dialysate to diffusate of the HBPs were appeared to be 70 : 30 and 87 :13 in the Meju fermented for 30 days and 80 days, respectively. and the rtio in the Doenjang aged for 60 days was 91 :9 , indicating that dialysate slowly days, respectively, and the ratio in the Doenjang aged for 60 days was 91 : 9, incidatin that dialysate slowly increased by the fermentation . Both portion exhibited strong antioxidant activity, but more stronger antioxidant activity was found in the dialysate. DEAE-celluose column chromatography showed that dialysate contained more materials eluted by 0.01-0.03M HCI solution than 0.01M acetate buffer, but diffusate showed opposite results. The degree of browning reaction and antioxdiant activity found in the fraction eluted by HCI solution was stronger than that of the fraction eluted by acetate buffer. The fraction eluted from DEAE-cellulose column chormatogrphy was further fractionaged by TLC and found that strong antioxidant activity was present in the fractions which did not possess fluorescenece and showed a negative ninhydrin reaction. TLC fractions of HCI eluant in Meju exhibited a strong absorbance at 260-280nm, but most of other fractions did not show any absorbance at UV region. TLC fractions from dialysate and diffusate showed fairly identical IR spectrum with absorbance at 3400cm-1, 2800cm-3000cm -1, 1600cm, -1 1400cm-1 and 1100 cm-1 , however, in addition to these absorbances, the spectrum from HCI eluant of Meju exhibited a strong absorbance at 1750cm , indicating the carbonic acid or carbonate ester.

• 한국약용작물학회지
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• 제24권3호
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• pp.191-197
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• 2016

Background: This study examined the use of new bio-materials with enhanced value and functionality, which were derived from fermented wild ginseng cultures. Methods and Results: To examine the antioxidant activity associated with biological functions, radical scavenging analyses (2,2-diphenyl-1-picrylhydrazyl, DPPH and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, ABTS) and superoxide dismutase (SOD)-like activity analyses were conducted. Furthermore, the total phenolic and flavonoid contents of wild ginseng fermented with microorganisms (Leuconostoc mesenteroides, Bacillus circulans, Bacillus licheniformis and B. subtilis subsp. inaquosorum) were evaluated to determine the antioxidant activity increment. Regarding ginseng fermented with B. licheniformis, values of $70.6{\pm}1.4%$, $44.3{\pm}1.7%$, and $88.4{\pm}1.3%$ were measured using DPPH, ABTS, and SOD-like antioxdiant activity analyses, respectively. The total phenolic content in ginseng fermented with B. licheniformis was $184.5{\pm}0.9{\mu}g{\cdot}GAE/m{\ell}$, and the total flavonoid contents was $108.5{\pm}1.8{\mu}g{\cdot}QE/m{\ell}$ in ginseng fermented with L. mesenteroides. Conclusions: Of the four types of lactic acid bacteria examined, the use of B. licheniformis to ferment ginseng resulted in greatest increase in antioxidant activity. Therefore, ginseng fermented by microorganisms might be used to produce functional bio-materials.

• 한국식품영양과학회지
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• 제39권9호
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• pp.1346-1352
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• 2010

인삼의 천연배지에 상황버섯(Phellinus linteus), 영지버섯(Ganoderma lucidum) 및 노루궁뎅이버섯(Hericium erinaceum) 균사체를 접종한 후 10, 20, 30, 40, 50일 동안 배양된 인삼발효물 80% 에탄올 추출물에 대한 항산화활성을 살펴보았다. 총 폴리페놀 함량은 149.63~205.91 mg/g 범위로 영지버섯 균사체 인삼배양물에서 가장 높았다. DPPH법에 의한 항산화활성의 $IC_{50}$값은 1.16~6.48 mg/mL 범위로 노루궁뎅이버섯 균사체 인삼배양물에서 가장 높았고, ABTS에 의한 총 항산화력은 1.37~4.03 mg AA eq/g 범위로 상황버섯 균사체 인삼배양물이 가장 높았다. $\alpha$-Glucosidase 억제활성은 50일 배양된 상황버섯 균사체 인삼배양물에서 92.51%로 가장 높게 나타내었으며, tyrosinase 저해활성은 40일 배양된 영지버섯 균사체 인삼배양물에서 13.21%로 가장 높게 나타났다. 인삼을 균사체로 배양할 경우 40일 정도 배양하면 항산화활성이 증가된 인삼제품을 만들 수 있으리라 생각된다.

• 한국미생물·생명공학회지
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• 제36권1호
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• pp.55-60
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• 2008

유색미는 회색 빛 외에 붉은색 흑은 보라색 계통의 색소를 겨 부분에 함유하고 있다. 특히, 흑미는 예로부터 건강식품으로 여겨져 왔다. 흑미(흑진주벼)는 최근에 anthocyanin포함하는 생리활성 물질원으로서 관심이 증대되고 있다. Anthocyanin은 수용성의 색소로서 flavonoid계통의 대표 색소로 알려져 왔다. 흑미에 함유되어있는 Anthocyanin으로는 cyanidin 3-O-glucoside와 peonidin 3-O-glucoside, malvidin 3-O-glucoside, pelagonidin 3-O-glucoside, delphinidin 3-O-glucoside등이 알려져 있다. 본 연구에서는, 흑미로부터 amberlite XAD7 chromatography방법을 사용해서 anthocyanin을 추출 후 HPLC와 UV-Vis spectrophotometer를 이용하여 정량분석 및 정성분석을 실시하였다. 그 결과 흑미의 anthocyanin 추출물 중 95%가 cyanidin 3-O-glucoside, 5%가 peonidin 3-O-glucoside임을 확인하였다. 그리고 in vitro상의 여러 가지 실험방법을 통하여 anthocyani의 항산화 효과를 측정하였다. 그 결과 100g/ml 농도의 anthocyanin추출물은 90%이상 linoleic acid emulsion의 과산화를 억제하였고, 55.20%의 DPPH 자유 라디칼 제거효과, 54.96%의 superoxide 자유 라디칼 제거효과, 72.67%의 과산화수소 제거효과를 보였다. 그리고 또한 높은 환원 능력을 확인할 수 있었다. 표준 항 산화 물질로는 BHA, BHT, ${\alpha}-tocopherol$을 사용하였다. 이 결과로 보아 anthocyanin추출물은 새로운 항산화 물질로서 이용될 수 있을 것이다.