• 제목/요약/키워드: anticipation

검색결과 237건 처리시간 0.027초

Expression of orf7(oxi III) as dTDP-Glucose 4,6-Dehydratase Gene Cloned from Streptomyces antibioticus Tu99 and Biochemical Characteristics of Expressed Protein

  • Yoo, Jin-Cheol;Han, Ji-Man;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제9권2호
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    • pp.206-212
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    • 1999
  • The gene orf7(oxi III) was expressed using an E. coli system in anticipation that it would encode dTDP-glucose 4,6-dehydratase which is involved in the biosynthesis of the olivose moiety of chlorothricin produced from Streptomyces antibioticus Tu99. The solubility of the expressed protein increased up to 20% under optimal induction conditions. The expressed protein was purified from the E. coli BL 21(DE3) cell lysate by a 28.5-fold purification in two chromatography steps with a 38% recovery to near homogeneity. The molecular weight and N-terminal amino acid sequence of the purified protein correlated with the predicted mass and sequence deduced from the orf7 gene. The purified protein was a homodimer with a subunit relative molecular weight of 38,000 Dalton. The expressed protein was found to exhibit dTDP-glucose 4,6-dehydratase activity and be highly specific for dTDP-glucose as a substrate. The values of K'm and V'max for dTDP-glucose were 28 $\mu$M and 295 nmol $min^{-1} (mg protein)^{-1}$, respectively. dTTP and dTDP were strong inhibitors of this enzyme.$NAD^+$, the coenzyme for dTDP-glucose 4,6-dehydratase, was tightly bound to the expressed protein.

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품행장애 청소년과 정상 청소년의 자아방어기제에 관한 비교 연구 (A Comparison Study of Ego Defense Mechanisms between Conduct Adolescents and Normal Adolescents)

  • 송재호;진혜경;김봉석
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제20권3호
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    • pp.140-145
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    • 2009
  • Objectives : This study explored the differences in their ego defense mechanisms between adolescents with conduct disorder and normal adolescents. Methods : Subjects were 35 adolescents with conduct disorder and 44 normal adolescents. The Ewha Defense Mechanism Test (EDMT), consisting of 200 items and 20 scales, was administered, to examine the defense mechanisms of both groups of adolescents. Results : Normal adolescents presented statistically significantly higher scores on the reaction formation, controlling, suppression, anticipation, dissociation, and distortion scales than did adolescents with conduct disorder. Zn addition, adolescents with conduct disorder used neurotic defense mechanisms of both neurotic and mature levels less frequently than normal adolescents did. Factor analysis revealed that, normal adolescents had higher scores on ego-expansive factor scales and behavior control factor scales thanadolescents with conduct disorder did. Conclusion : The results suggest adolescents with conduct disorder use mature and ego-expansive defense mechanisms less frequently than do normal adolescents.

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Sensitivity Analysis of JLSP Inventory Model with Ordering Cost inclusive of a Freight Cost under Trade Credit in a Two-stage Supply Chain

  • Shinn, Seong-Whan
    • International Journal of Advanced Culture Technology
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    • 제8권3호
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    • pp.300-306
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    • 2020
  • This study analyzes the distributor's inventory model in a two-stage supply chain consisting of the supplier, the distributor and the end customer. The supplier will allow a credit period before the distributor settles the account with him in order to stimulate the demand for the product he produces. It is also assumed that the distributor pays the shipping cost for the order and hence, the distributor's ordering cost consists of a fixed ordering cost and the shipping cost that depend on the order quantity. The availability of the delay in payments from the supplier enables discount of the distributor's selling price from a wider range of the price option in anticipation of increased customer's demand. As a result, the availability of a credit transaction leads to an increase in inventory levels. On the other hand, in the case of deteriorating products in which the utility of the product perish over time, the deterioration rate with time plays a role in reducing inventory levels. In this regard, we analyze the effect of the length of the credit period and the degree of product deterioration on the distributor's inventory level. For the analysis, we formulate the distributor's annual net profit and analyze the effect of the length of credit period and deterioration rate of the product on inventory policy numerically.

Critical Design Issues on the Cathodic Protection Systems of Ships

  • Lee, Ho Il;Lee, Chul Hwan;Jung, Mong Kyu;Baek, Kwang Ki
    • Corrosion Science and Technology
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    • 제6권3호
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    • pp.90-95
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    • 2007
  • Cathodic protection technology has been widely used on ship's outer hull and inner side of ballast water tanks as a supplementary corrosion protection measure in combination with protective organic coatings. Impressed current cathodic protection system is typically opted for the ship's hull and, sacrificial anode system, for ballast water tanks. The anticipation and interest in cathodic protection system for ships has been surprisingly low-eyed to date in comparison with protective coatings. Computational analysis for the verification of cathodic protection design has been tried sometimes for offshore marine structures, however, in commercial shipbuilding section, decades old design practice is still applied, and no systematic or analytical verification work has been done for that. In this respect, over-rotection from un-erified initial design protocol has been also concerned by several experts. Especially, it was frequently reported in sacrificial anode system that even after full design life time, anode was remaining nearly intact. Another issue for impressed current system, for example, is that the anode shield area design for ship's outer hull should be compromised with actual application situation, because the state-of-the-art design equation is quite impractical from the applicator's stand. Besides that, in this study, some other critical design issues for sacrificial anode and impressed current cathodic protection system were discussed.

The Emerging Application Potential of LTPS Technology

  • Yoneda, Kiyoshi;Yokoyama, Ryoichi;Yamada, Tsutomu;Mameno, Kazunobu
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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    • pp.43-49
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    • 2003
  • Low-temperature polysilicon (LTPS) technology has continued to mature with the passing of each year since LTPS mass production began. The integration of complex circuits has become possible with advances in microprocessing, leading to the realization of panels with highly advanced functions. At the same time, efforts have been made to meet market demands for lower costs, thereby boosting competitive strength. Today, LTPS-TFT LCDs have become standard equipment for the monitors of digital still cameras, and inroads are being made into the massive cellular phone market. Micro displays such as electronic viewfinders, which were previously only possible with high-temperature polysilicon technology, can now also be made with LTPS, thus expanding the scope of the technology. AMOLED displays using the LTPS-TFT as a back plane are also approaching the stage of industrialization. The hidden potential for the OLED to replace the familiar LCD has prompted Widespread anticipation for this emerging technology. This paper reflects on the history of LTPS technology, then looks forward to its future prospects and suggests a variety of potential fields of application.

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자전거 프레임 포크에서의 진동 해석 (Vibration Analysis at Bike Frame Fork)

  • 조재웅;한문식
    • 한국기계가공학회지
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    • 제13권1호
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    • pp.8-15
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    • 2014
  • This study investigates structural and vibration analyses for three types of bike frame fork models. As long as the maximum equivalent stresses of these models are lower than the yield stress, the three models are considered to be safe structurally. Type 3, with a maximum equivalent stress of 169.23 MPa, has the lowest stress among the three models and the strongest strength. Types 1, 2 and 3 have natural frequencies lower than 270 Hz. Type 3, with a critical frequency of 118 Hz, has the best durability under vibration among the three models. In order to decrease the vibration transmitted to a bike rider riding on a rough road, the impact due to vibration can be relieved by selecting a Type 3 model from among the three models. The results of this study can be effectively utilized for the design of a bike frame fork as this allows the anticipation and prevention of damage caused by durability issues.

교통 예비점멸등 사용에 따른 운전자 행동반응 비교 (A Comparison Study of Driver's Responsive Action by Using the Traffic Light Change Anticipation)

  • 장명순;김영준
    • 대한교통학회지
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    • 제21권6호
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    • pp.67-73
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    • 2003
  • 본 연구는 기존의 신호등의 시각적 인지도를 향상시켜 운전자의 인지실수를 예방하고 안전운전을 도모하기 위해 보조녹색신호가 추가된 신호등에 대한 연구를 수행하였다. 기존의 3색, 4색 신호등과 보조녹색신호를 추가로 제시한 3색, 4색 신호등을 자동차 모의실험장치를 통하여 비교한 결과 두 가지의 신호등간의 유의한 차이가 있는 것으로 나타났다. 이러한 결과는 개선된 신호등이 현장 적용시 운전자가 스스로 의식하지 못하는 상태에서 제동시점을 다소 앞당겨준다는 점에서 안전운전에 기여할 수 있는 가능성을 보여주었다. 본 연구 결과는 실제 운전자의 반응을 비교함과 동시에 주관적인 행동반응을 조사함으로써, 연구결과의 실제 적용가능성을 제시하였다는데 그 의의가 있다고 할 수 있다.

An exosolar planetary system N-body simulator II

  • Hong, ChaeLin;van Putten, Maurice
    • 천문학회보
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    • 제43권1호
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    • pp.46.3-47
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    • 2018
  • We present a general N-body exasolar system simulator in anticipation of upcoming searches for exoplanets and even exomoons by next generation telescopes such as James Webb Space Telescope. For habitable zones, traditionally defined by temperature, we here address the essential problem of dynamical stability of planetary orbits. Illustrative examples are presented on P-type orbits in stellar binary systems, that should be fairly common as in Kepler 16b. Specific attention is paid to reduced orbital lifetimes of exoplanets in the habitable zone by the stellar binary, that is propoesed by Maurice van Putten (2017). Especially, we focused on a classic work of complex three-body problem that is well known by Dvorak(1986). We charge his elliptic restricted three-body problem to extend unrestricted three-body problem to look into dynamical motions in view of circumbinary planet, furthermore, we suggest that opposite angular orientation of the planet is relative to the stability of orbits. In here, counter-rotation case is relatively more faster than co-rotation case for being stable. As a result, we find that various initial conditions and thresholds to approach dynamical stability and unstability with unexpectable isolated islands over enormous parameter space. Even, superkeplerian effect of binary is important to habitability of the exoplanet and we can verify that superfaster binary doesn't effect on th planet and increases survivality of planet around the binary.

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사회 안전망을 위한 위기관리시스템 설계 (Design of Emergency Management System for Societal Security Network)

  • 김윤호;강희조
    • 한국항행학회논문지
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    • 제16권5호
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    • pp.879-884
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    • 2012
  • 산업화가 고도화 되면서 인류는 자연재해는 물론이고 기술적 실수, 금융문제 등, 다양한 위기를 경험하고 있다. 국제표준에 근거한 위기관리 시스템 설계는 조직이 예방, 대비 및 대응을 강화시킬 수 있는 가능성을 점진적으로 증가시킬 수 있는 기본 틀을 제공해 준다. 본 연구에서는 사회 안전망을 위한 다양한 분야의 위기관리 시스템 설계를 소개하였다. 먼저 ISO/TC223의 역할과 현재 진행 중인 표준화 이슈들을 정리하였고 잠재적 위험을 저감시키며 조직의 회복력을 증가시킬 수 있는 효과적인 위기관리 시스템을 설계하였다.

현대 가구에서 나타나는 재료의 지속가능성에 대한 연구 (A Study of The Modern Furniture's Material and Sustainable)

  • 백은;서명원
    • 한국가구학회지
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    • 제27권2호
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    • pp.80-90
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    • 2016
  • The idea of Sustainable, first mentioned in "Our Future (Bruntdland)" from the United Nations report released in 1987 has been highly affect design disciplines in developed countries in 21st century. It relocated industrial society and structure's attentions to nature and recourse conservation and reuse. Designed and manufactured products eventually disposed and it leads to waste of resources as well as creates environmental issues. Unfortunately, vast majority of designers starts designing without anticipation of this matter and produce more believing this leads to more sales. However shorter life of a product will create bigger increase of resource consumption and waste than, importance of re-usage and recycle will eventually increase. In modern furniture industry metal and plastics are frequently used beside wood that material has been diverse than in past. However, there are lack of plans and regulations of re-usage of those material due to difficulties of separating each different materials and collecting due to it's size. This study would like to look into recycling rate, methods and usage of three major materials (wood, metal, plastic) that is typically used in current furniture industries. Furthermore, see the potential of sustainable furniture by analyzing furniture practices made by each different recycled material from old furniture and what designers should consider to achieve feasibility of sustainable furniture.