• Journal of the Korean Wood Science and Technology
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• 제33권2호통권130호
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• pp.72-77
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• 2005

Streptococcus mutans is known as a strong cariogenic bacterium and produces dental caries. This study was carried out to develop anticariogenic agents from Callistemon citrinus. Crude extracts and thier various solvent fractions of the plant extracts were tested for the antibacterial activity against S. mutans. The anticariogenic activity was evaluated with by paper disc diffusion method and minimal inhibition concentration (MIC). The extract from the fruit and its hexane fraction showed a strong inhibitory effect on the growth of S. mutans and the MICs were 31.2 and $3.9{\mu}g/m{\ell}$, respectively.

• 생약학회지
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• 제33권4호통권131호
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• pp.319-323
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• 2002

This study was carried out to develop anticariogenic agents from natural products, the methanol and ethyl acetate extracts of 105 medicinal plants were tested for the antimicrobial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. Among them, the Aristolochia contorta, Dryopteris crassirhizoma, Eugenia caryophyllata, Hemerocallis fulva, Pinellia ternata, Pyrola japonica, and Scrophularia buergeriana showed significant antimicrobial activity.

• 대한소아치과학회지
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• 제23권2호
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• pp.306-326
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• 1996

The purpose of this study was to compare the enamel fluoride uptake and anticariogenic effect following topical application with different physical form of fluoride and application time. The results can be summarized as follows: 1. While the 4-minute applicaton increased the enamel fluoride uptake significantly than 1-minute application regardless of physical form of applied topical fluoride agents, no siginificant difference could be found between foam and gel type when applied for the identical period of time. 2. The application time or the physical form of topical agents had no singificant influence on the reduction of caries lesion. 3. Based upon the above-mentioned results of this study, it cannot be concluded that the amount of enamel fluoride uptake has an absolute impact on the change of lesion depth. Therefore, the clinical application of 1-minute topical fluoride application which appears to have a similar anticariogenic efficacy to 4-minute fluoride application may be encouraged.

• 대한소아치과학회지
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• 제29권4호
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• pp.625-631
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• 2002

임상에서 사용하고 있는 8종의 항우식 작용을 갖는 항균물질의 조합이 대표적 우식유발 세균인 Streptococcus mutans Ingbritt와 Streptococcus sobrinus 6715-7의 성장에 미치는 영향을 조사하고자 세균별로 28개 조합의 각 항균물질에서 최소 저해 농도와 분할 저해 농도 지수를 구하여 여러 기준에 따라 상승작용, 길항작용, 무관함, 부분적 상호작용 등을 각각 평가하였다. 미국 미생물 학회의 지침에 따라 분류하면 약 34%의 조합에서 상승작용이 관찰되었고 Berenbaum의 분류에 따르면 약 82%에서 상승작용을 갖는다고 해석할 수 있었다. 또한 Isenberg가 정의한 부분적 상승작용은 총 조합수의 절반에서 관찰되었다. 분류기준에 따라 다양한 결과를 얻을 수 있었으나 두 가지 항균물질의 조합이 우식유발 세균을 억제하는데 상승작용을 나타내는 경향이 존재함을 관찰할 수 있었고 따라서 우식유발세균을 억제하는데 있어서 항균물질의 단독 사용보다 세균의 생태와 대사의 여러 부분에 영향을 미치는 항균물질의 조합은 유용할 수 있다는 것을 알 수 있었다.

• 생약학회지
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• 제47권1호
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• pp.43-48
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• 2016

The aim of the study was performed to examine the anticariogenic potential of purified bee venom (Apis mellifera L., PBV) collected using bee venom collector from cariogenic bacteria, Streptococcus mutans, Streptococcus sanguis, Porphyromonas gingivalis, and Fusobacterium nucleatum. The anticariogenic effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). The human lower gingiva epithelial cell cytotoxicity of purified bee venom was also evaluated. Purified bee venom exhibited significant inhibition of bacterial growth of S. mutans, S. sanguis, P. gingivalis, and F. nucleatum with MIC value of 0.68, 0.85, 3.49, and $2.79{\mu}g/ml$, respectively. The MBC value of purified bee venom against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum was 1.34, 1.67, 8.5, and $6.8{\mu}g/ml$. Furthermore, the results of PAE values against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum showed the bacterial effect with 3.3, 3.45, 2.0, and 2.0. The concentration below 1 mg/ml of purified bee venom had no cytotoxicity in the human lower gingiva epithelial cell. These results suggested that purified bee venom have great potenial as anticariogenic agents.

• 약학회지
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• 제35권1호
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• pp.7-10
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• 1991

For the purpose of developing of anticariogenic agents, P-phenylphenol derivatives were synthesized and determined their antibacterial activities against a cariogenic bacterium, Streptococcus mutans. Among synthetic compounds, 2-nitro-6-bromo-p-phenylphenol showed as potent antibacterial activity as magnolol and honokiol.

• 한국응용과학기술학회지
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• 제37권2호
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• pp.279-286
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• 2020

치아우식증 치료물질을 개발하기 위하여 한국에서 전통적으로 널리 사용되어왔던 20종류의 약용식물로부터 추출물을 분리하였다. 이를 이용하여 치아우식증 원인균인 Streptococcus sobrinus KCOM 1157에 대한 항균 활성을 나타내는 약용식물들을 조사하였다. 감초, 고삼, 황련, 박하, 송절, 부평초 등의 추출물들이 S. sobrinus KCOM 1157에 대해서 항균 활성을 보여주었다. 이 중 송절, 고삼, 황련 등의 천연물들은 높은 항균 활성을 나타내었다. 이 결과들은 송절, 고삼, 황련의 천연물들이 S. sobrinus 를 포함한 구강질환 세균들에 대한 항우식증 치료제로서의 가능성이 있음을 의미한다.

• Food Science and Biotechnology
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• 제14권2호
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• pp.286-289
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• 2005

Active antibacterial compound against periodontitis-causing bacterium Porphyromonas gingivalis was isolated from Kaempferia pandurata and identified as panduratin A. Minimum inhibitory concentration (MIC) value of panduratin A was $4\;{\mu}g/mL$, much lower than those of other natural antibacterial agents. Panduratin A also showed antibacterial activity against Prevotella intermedia ($2\;{\mu}g/mL$), P. loescheii ($4\;{\mu}g/mL$), and cariogenic Streptococcus mutans ($4\;{\mu}g/mL$). Damage on cell wall and perturbation of cytoplasmic membrane of panduratin A-treated P. gingivalis were visualized through transmission electron microscopy. These results suggest panduratin A, exhibiting strong and preferential antiperiodontal and anticariogenic activities, may be utilized in functional foods for prevention of oral diseases.

• 약학회지
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• 제36권1호
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• pp.36-39
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• 1992

In a continuous study for the developing of the anticariogenic agents, 3,3'-diacyl-4,4'-biphenol derivatives (Fig. 2, 2 and 3) and 3,3'-bis-(1-hydroxyalkyl)-4,4'-biphenol (Fig. 3, 4 and 5) derivatives are synthesized successively from 4,4'-diphenol (Fig. 2, 1). The synthesized compounds are tested for their antibacterial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. The acyl derivatives, 2 and 3, do not show antibacterial activity, but the hydroxyalkyl derivatives, 4 and 5, reduced from the acyl group of 2 and 3, show the activity. The antibacterial activity of 2 and 3 may be inhibited due to intramolecular hydrogen bonding between the acyl group and the hydroxyl one (Fig. 4).

• 생약학회지
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• 제28권3호
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• pp.99-103
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• 1997

To develop anticariogenic agents, Scutellariae Radix was tested for its antibacterial activity against Streptococcus mutans OMZ176 which is known as a strong cariogenic bacterium. The antibacterial activity was evaluated with disk plate method and minimal inhibitory concentration (MIC). The ethyl acetate fraction showed significant activity. By means of activity-guided fractionation, baicalein and ganhuangenin were isolated as the active principles. The MICs of these compounds were $100{\mu}g/ml$ and $200{\mu}g/ml$, respectively.