• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1324-1329
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• 2007

Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring, multi-biofunctional chemical existing in grapes and various other plants as a polyphenol type, and it is one of the best known natural anticancer and antiatherosclerosis reagents. In this study, we investigated the antifungal action by resveratrol in Candida albicans, which is a human infectious fungi as an agent of candidiasis. Resveratrol displayed potent fungicidal activity in an energy-dependent manner, without any hemolytic effects against human erythrocytes. It was found that the serum-induced mycelial forms, which playa crucial role in the pathogenesis of C. albicans during host tissue invasion, were disrupted by resveratrol. To understand the correlation between lethal effects and resveratrol action, we examined the physiological changes of C. albicans. A significant accumulation of intracellular trehalose was induced by stress responses to resveratrol action, and a remarkable arrest of cell-cycle processes at the S-phase in C. albicans occured. Therefore, the fungicidal effects of resveratrol demonstrate that this compound is a potential candidate as an antifungal agent in treating infectious diseases by candidal infections.

• Journal of Nutrition and Health
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• 제44권6호
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• pp.465-473
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• 2011

Dietary fatty acids are under intense research to identify anti-atherogenic mechanisms, so we investigated green tea powder (GT) as a protector against atherogenesis originating from lipid peroxidation such as 4-hydroxynonemal (4-HNE) and malondialdehyde (MDA) in different dietary fatty acid-treated apo E KO mice. Growth rate and dietary efficiency were lower in apo E KO mice with or without LA compared to wild type. Plasma total cholesterol (TC) and triacylglycerol (TG) did not correspond to values in other tissues, but TG in heart tissue decreased significantly by GT after linoleic acid (LA) or docosahexaenoic acid (DHA) was administered. LA induced apoptosis as evidenced by changes in aorta morphology and immunohistochemistry. Lipid peroxides (LPO) was increased in apo E KO mice with or without LA corresponding to the accumulation of 4-HNE or MDA in the proximal aorta above the atria. GT consumption tended to reduce the primary causal mechanism of atherogenic phenomena such as oxidizability in both LA and DHA treated atherogenic mice. A high polyunsaturated fatty acids (PUFA) diet involved the changes on stress-induced apoptotic signaling by increasing caspase 3, cytochrome c, and nuclear factor-${\kappa}B$ in the heart tissue, but decreasing the bcl-2 protein. However, GT remarkably reduced the expression of apoptotic signaling, in contrast to the PUFA diet. Therefore, the potential of GT as an anti-atherosclerotic dietary antioxidant was tested in this study.

• Journal of Ginseng Research
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• 제39권2호
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• pp.155-161
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• 2015

Background: Korean ginseng is an ethnopharmacologically valuable herbal plant with various biological properties including anticancer, antiatherosclerosis, antidiabetic, and anti-inflammatory activities. Since there is currently no drug or therapeutic remedy derived from Korean ginseng, we developed a ginsenoside-enriched fraction (AP-SF) for prevention of various inflammatory symptoms. Methods: The anti-inflammatory efficacy of AP-SF was tested under in vitro inflammatory conditions including nitric oxide (NO) production and inflammatory gene expression. The molecular events of inflammatory responses were explored by immunoblot analysis. Results: AP-SF led to a significant suppression of NO production compared with a conventional Korean ginseng saponin fraction, induced by both lipopolysaccharide and zymosan A. Interestingly, AP-SF strongly downregulated the mRNA levels of genes for inducible NO synthase, tumor necrosis factor-${\alpha}$, and cyclooxygenase) without affecting cell viability. In agreement with these observations, AP-SF blocked the nuclear translocation of c-Jun at 2 h and also reduced phosphorylation of p38, c-Jun N-terminal kinase, and TAK-1, all of which are important for c-Jun translocation. Conclusion: Our results suggest that AP-SF inhibits activation of c-Jun-dependent inflammatory events. Thus, AP-SF may be useful as a novel anti-inflammatory remedy.

• 생명과학회지
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• 제19권8호
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• pp.1145-1151
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• 2009

신의대 (Sasa corean, Nakai, 대나무 일종) 추출물이 혈청지질 개선에 미치는 영향을 조사하기 위하여 1.0%의 콜레스테롤 첨가식이로 유발된 고지혈증 Sprague Dawley계 수컷 흰쥐를 이용하였다. 실험동물들은 4 종류 투여군으로 나누었으며 고콜레스테롤 투여군(HC)을 대조군으로 하였고 3 종류의 대나무추출액을 첨가한 투여군(HCW, HCM 및 HCH) 을 실험군으로 하였다. 대나무추출액은 헥산, 메탄올 및 물을 이용하여 순차적으로 추출하여 농축, 동결건조하여 사용하였다. 고콜레스테롤식이를 급여한 대조군(HC 대조군)에 비하여 대나무추출물을 첨가한 모든 투여군(HCW, HCM 및 HCH 투여군)에서 체중변화 및 식이섭취량에 미치는 영향은 별로 크지 않았다. 혈청 총 콜레스테롤 및 LDL-콜레스테롤 함량을 비교하였을 때 HC 대조군에 비하여 대나무추출물 첨가 투여군에서 18.5-23.7% 및 15.7-18.6% 감소하였으며 특히 메탄올추출물의 효과가 가장 높았다. 항동맥경화 인자로 알려진 HDL-콜레스테롤의 경우 대나무추출물 투여군에서 HC 대조군에 비하여 17.2-21.9% 정도 증가하는 것을 볼 수 있었다. 대나무추출물은 고콜레스테롤 식이를 섭취한 HC 대조군에 비하여 동맥경화지수를 낮추는 효과가 있었다(HC group : 2.96${\pm}$0.08, HCM group : 1.48${\pm}$0.02, HCW group : 1.69${\pm}$0.04, HCH group : 1.84${\pm}$0.01). 또한 혈청중의 중성지질 함량 및 인지질 함량도 메탄올추출물 투여군에서 가장 낮았다. 이러한 실험 결과들로부터 대나무 추출물, 특히 메탄올추출물이 혈청지질의 개선효과가 있으며 결과적으로 고지혈증이나 심장순환기계 질환을 예방하는 효과가 있을 것으로 사료된다.