• 한국응용과학기술학회지
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• 제33권2호
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• pp.361-373
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• 2016

본 연구는 오래전부터 민간요법으로 암의 치료제로 사용된 약초인 와송 추출물의 항산화 활성과 항노화 효능을 검증하였다. 와송 추출물의 항산화 활성을 측정한 결과, 총 폴리페놀 함량은 100 g의 와송 추출물 건조중량 당 12 g의 높은 caffeic acid 당량이 함유되어 있는 것을 확인하였고, 총 플라보노이드 함량은 100 g의 와송 추출물 건조중량 당 1.7 g의 quercetin 당량이 함유되어 있는 것을 확인하였다. DPPH free radical 소거능은 와송 추출물 1%의 농도에서 79.7%의 높은 소거능을 확인하였다. 또한 와송 추출물의 항노화 효능 측정 결과 $25{\mu}g/mL$, $50{\mu}g/mL$ 농도에서 control과 유사한 MMP-1 발현 억제능을 확인하였다. 와송 추출물의 임상실험결과 와송 추출물 함유 화장품 사용군의 수분함량은 오른쪽 볼과 코, 턱의 부위에서 통계적으로 유의하게 증가하였고, 정상적인 피부의 유분 함량과 pH를 유지하는 결과를 확인하였다. 이상의 결과에 따라 와송 추출물은 항산화와 항노화 효능을 가진 천연 화장품 소재로서의 활용 가능성이 있다고 사료된다.

• 한국환경보건학회지
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• 제22권4호
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• pp.109-121
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• 1996

It was intended to investigate the effect of the microbiological kitchen hygiene such as dishclothes and scrubbers. The 8indicator organisms (standard plate counts, coliform, heterotroph, enterococcus, staphylococcus, heat-stable bacteria, psychrotroph, Pseudomonas aeruginosa) were detected highly in dishwaters, dishcloth and scrubber. Coliform and Staphylococcus aureus were appeared on dishcloth dominantly than the scrubber, and the scrubbers were intruded by hetrotrophs and psychrotrophs numerously than dishclothes. The germ-resistant sponge inhibited the growth of the most of test strain, and appeared the about 100% reduction rate after 24 hr, but did not affect Pseudomonas aeruginosa and P. fragi so typically after 24 hr. The anti-microorganism durability of germ-resistant sponge, treated with food soil, was maintained by 10 days, the early stage strain density was founded in 20 days, and the strains grew after 30 days.

• 한국응용과학기술학회지
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• 제35권3호
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• pp.975-984
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• 2018

마치현 추출물에 대한 생리활성 소재로서 응용 가능성을 확인하고자 하였다. 마치현 추출물의 측정 결과, 플라보노이드, 폴리페놀 함량과 DPPH radical 소거능을 확인하였으며, 세포실험 결과 HaCaT, RAW 264.7, RBL-2H3 세포에서 유의한 세포독성은 나타나지 않았으며. $H_2O_2$에 의한 유발되는 산화적 스트레스에 대한 HaCaT 세포는 마치현 에탄올 $100{\mu}g/mL$ 농도에서 83% 보호효과가 확인되었다. RAW 264.7 세포에 대한 마치현 추출물의 항염 효과를 확인 결과 저농도에서도 nitric oxide 생성이 억제되었으며, S. aureus, S. epidermidis, P. acnes 균에서도 마치현 추출물의 농도 의존적으로 항균 활성이 확인되었다. 본 연구는 마치현 추출물의 항산화, 항염증 및 항균 효과를 가지는 생리 활성 물질로서 활용 가능성을 확인하였다.

• 동아시아식생활학회지
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• 제19권2호
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• pp.202-209
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• 2009

This study was conducted to gather the basic data on the spread of the domestic kiwifruits, and the development of the manufactured goods and the health functional foods produced using kiwifruits. We determined the chemical compositions of four types of kiwifruits cultivated in Korea, Daeheung, Bidan, Haegeum and Hayward. In addition, we measured the anti-microbial activities and cytotoxicities of these types of kiwifruits. The vitamin C contents of the kiwifruits increased in the order of Bidan (93.82 mg/100 g), Daeheung (85.89 mg/100 g), Haegeum (83.73 mg/100 g) and Hayward (75.28 mg/100 g). The total amino acids contents per 100 g of kiwifruit (dry weight basis) were 483.97 mg (Haegeum), 453.08 mg (Hayward), 437.27 mg (Bidan) and 369.35 mg (Daeheung). The K and Ca contents of the kiwifruits ranged from 14.56~37.12 mg/L and 1.94~8.24 mg/L, respectively; however, the Fe, Mg, Zn and Cr contents all less than 1.83 mg/L. The antimicrobial activities of methanol extracts of kiwifruits against five gram positive bacteria at concentration of 2,000 mg/L in terms of inhibition diameter ranged from 8.8~12.8mm, while raged from 9.2~13.1mm against three gram negative strains of bacteria. The hyperplasia inhibition of lung cancer cells (Calus-6) by 800 mg/L kiwifruits extracts of Bidan, Haegeum, Daeheung and Hayward kiwifruits were 21.2%, 9.5%, 6.7% and 5.0%, respectively. Consequently, it was assumed that kiwifruits was rich in vitamin C, amino acids and K, and that they would therefore be useful in processed goods.

• KSBB Journal
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• 제29권1호
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• pp.50-57
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• 2014

NF-${\kappa}B$ is a transcriptional factor which is involved in many biological processes including immunity, inflammation, and cell survival. Many investigators studied on the mechanism involved in activation of NF-${\kappa}B$ signalling pathway via ubiquitination and degradation of $I{\kappa}B$ regarding skin disease. Some specific molecules including Akt, MEK, p38 MAP Kinase, Stat3, et al. represent convergence points and key regulatory proteins in signaling pathways controlling cellular events such as growth and differentiation, energy homeostasis, and the response to stress and inflammation. Ultraviolet (UV) irradiation has many adverse effects on skin, including inflammation, alteration in the extracellular matrix, cellular senescence, apoptosis and skin cancer. Prunus mume, a naturally derived plant extract, has beneficial biological activities as blood fluidity improvement, anti-fatigue action, antioxidative and free radical scavenging activities, inhibiting the motility of Helicobacter pyolri. Previous reports on various beneficial function prompted us to investigate UVB-induced or other immunostimulated biological marker regarding P. mume extract. P. mume extract suppresses UVB-induced cyclooxygenase-2 (COX-2) expression in mouse skin epidermal JB6 P+ cells. The activation of activator protein-1 and nuclear factor-${\kappa}B$ induced by UVB was dose-dependently inhibited by P. mume extract treatment. This results suggest that P. mume extracts might be used as a potential agents for protection of inflammation or UVB induced skin damage.

• 한방재활의학과학회지
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• 제29권4호
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• pp.15-27
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• 2019

Objectives This study was performed to evaluate anti-obesity effects of Crataegus pinnatifida (CP) on high-fat-diet induced obese mice. Methods The experimental animals were divided into four groups: normal diet (NOR) group, high fat diet (HFD) group, HFD+Xenical (XEN) group, and HFD+CP (CP) group. NOR group was fed a normal diet and the other three groups were fed high fat diet during the experiment. After the first two weeks of diet, XEN group and CP group were administered with XEN or CP for seven weeks, respectively. After that, we measured body weight, liver weight, fat weight, food intake, and serum concentrations of lipids and liver enzymes. Also the liver, intestine, fat tissue was removed to estimate the obesity-related mRNA expressions and the stool sample was collected to analyze the gut microbiota. Results We found that body weight, fat weight, and triglyceride level were decreased significantly in CP group compared to HFD group. Also CP significantly suppressed gene expressions associated with lipogenesis and inflammation, and increased gene expressions of browning of white adipose tissue and mitochondrial biogenesis. Moreover, it shifted the microbial diversity closer to that of NOR group and increased Firmicutes/Bacteriodetes ratio. Conclusions These results suggest that CP decrease body weight, fat weight and serum triglyceride. Also it inhibit inflammation and adipogenesis, altering gut microbial diversity and abundance. In conclusion, CP could be used as a therapeutic drug for obesity via gut microbiota modulation.

• 한방비만학회지
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• 제18권2호
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• pp.96-105
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• 2018

Objectives: The aim of this study was to examine the effect of Asparagus cochinchinensis (AC) and fermented AC (fAC) on microorganisms and efficacies. Methods: AC was fermented for four weeks without using any bacterial strains. Then we investigated fermentation characteristics including potential of hydrogen (pH), total sugar, microbial profiling and antioxidant compound contents such as total polyphenol and total flavonoid. The anti-obesity effects of AC and fAC were evaluated by using Oil Red O staining in 3T3-L1 adipocyte. Also anti-diabetic effects of them were evaluated by using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) uptake in C2C12 skeletal muscle cell. Results: Both pH and total sugar of fAC were decreased significantly compared to unfermented AC. And the abundance of total bacteria and lactic acid bacteria increased during fermentation, especially Lactobacillus plantarum. Also fermentation of AC increased the content of total polyphenol. On the metabolic aspects, we found that AC and fAC suppressed fat accumulation. Conclusions: After four weeks of fermentation, AC increased concentrations of active compounds, altered microbial composition, and inhibited fat accumulation such as triglyceride. These results indicate that fermentation of AC might be a beneficial therapeutic approach for obesity.

• Applied Biological Chemistry
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• 제50권3호
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• pp.210-216
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• 2007

방사선 조사한 녹차 폴리페놀을 스킨과 에센스에 첨가하여 방부제를 첨가하지 않았을 때의 항균효과 및 방부효과를 검증하고 무방부제 화장품으로서의 가능성을 검토하였다. 항균력 측정 결과, C. albicans를 제외한 모든 균주에서 항균효과가 나타났으며, 피부상재균인 S. aureus에서 항균효과가 높게 나타났다. MIC 측정 결과, E. coli와 C. albicans의 경우 2,500 ppm에서, S. aureus의 경우 1,000 ppm에서 저해농도를 나타내었으며, MBC 측정 결과, C. albicans를 제외한 모든 균주에서 측정이 가능하였으며, E. coli의 경우 10,000 ppm, S. aureus의 경우 5,000 ppm에서 모든 균이 사멸하였다. 시간에 따른 생균수 측정 결과, 시간이 지남에 따라 균의 증식을 억제하여 항균력이 뛰어남을 알 수 있었다. 이와 같은 결과로 미루어 보아 녹차폴리페놀은 스킨과 에센스에 첨가 시 항균효과가 우수하여 화장품 첨가제로 이용이 가능할 것으로 사료된다.

• 한국식품영양과학회지
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• 제28권1호
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• pp.161-166
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• 1999

CaCl2 and water soluble degraded chitosan mixtures were used as coagulants for extending the shelf life of tofu. Microbial counts, pH, and turbidity in tofu immersion solution were analyzed during the periods of storage at 4oC and physico chemical properties of tofu were determined by rheological properties, color and yield. There was no significant difference in moisture content and yield between tofu made with and without chitosan. Failure stress and stress relaxation values were reduced when chitosan was added to CaCl2, but there was no significant change among contents of chitosan added to tofu. The pH values were decreased at the beginning and then slowly increased after 14 days storage. The changes in turbidity were increased during storage, but the change in 2.0g degraded chitosan mixture with 7.0g CaCl2 coagulant(2.0:7.0 ratio) was lower than others and initial turbidity was lower as much as chitosan added to coagulant. These data showed that the water soluble degraded chitosan had a good anti microbial effect and increases the shelf life of tofu.

• 한국수소및신에너지학회논문집
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• 제33권4호
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• pp.437-445
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• 2022

This paper presents an experimental study on the main management factors of the condenser contamination such as fouling and corrosion for the R-134a turbo-chiller to save energy, reduce corrosion rates, and reduce maintenance costs through the application of condenser non-cleaning water treatment chemical. The series of experiment is conducted using combining oxidative microbial sterilizers, non-oxidizing microbial sterilizers, and anti-corrosion agents. The leaving temperature difference and corrosion rates for three different combination of chemicals are collected and analyzed. The experimental results show that the cost reduction (4,066,000 Won/year) of the disinfectant (FT-830) can be achieved by adding the oxidative disinfectant (NaOCl) and the non-oxidizing disinfectant (NX-1116). The LTD value is maintained at 1.9℃, and the corrosion rates of copper and carbon steel specimens are 0.07 mpy and 1.61 mpy, respectively.