• Title/Summary/Keyword: anti microbial susceptibility RT-PCR

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Molecular Epidemiological Analysis of Outbreaks to Pathogenic Salmonella othmarschen Using PFGE (Salmonella othmarschen에 의한 집단식중독사례의 분자역학적 분석)

  • Bang, Seon-Jae;Park, Kwang-Hee;Park, Ki-Myung;Kwon, Yeon-Ok;Ko, Hoan-Uck
    • Journal of Environmental Health Sciences
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    • v.34 no.2
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    • pp.170-174
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    • 2008
  • The strains of Salmonella othmarschen and Norovirus isolated from outbreak A, the funeral ceremonies incidents in Ouri-shi of Oyeonggido in, 2007, were subjected to genotyping by using PFGE and extraction of RNA by using RT-PCR. 24 Salmonella othmarschen were isolated from 37 cases (patients 12 cases, cookers 4 cases, foods 7cases, knife, kitchen board, dish towel 4 cases, etc) and PFGE patterns showed that 22 strains isolated from the same incident were almost of the same pattern and 2 strains exhibited 50% of the pattern. Antimicrobial susceptibility patterns were such that there were 22 case susceptibility patterns and 2 case resistance patterns (strain 4: AM, CF, CIP, NA, TE TIC and strain 13: CF, CZ). Norovirus G1 (1case), Norovirus G2 (7cases), Norovirus G1,G2 (1case) were detected by using RT-PCR. The fine typing ability and reliability of PFGE and BioNumerics are helpful for establishing out the foodborne pathogens of outbreaks.