• Journal of Ginseng Research
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• v.8 no.1
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• pp.45-56
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• 1984

This study was carried out to obtain the basic information on the development of flower bud and to clarify the characteristics of flower organ and flowering in Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolium). The formation of flower bud in the dormancy bud of Korean ginseng was initiated about the middle of June and completed late in September. The ovary, style and anther of Panax ginseng, violet-stem and yellow-berry variants, were formed earlier than those of Panax quinquefolium. Panax ginseng, therefore, flowered earlier by one month in comparison with Panax quinquefolium. As for the effect of temperature on the flowering of ginseng, both species, Panax ginseng and Panax quinquefolium, grown at 20 $^{\circ}C$ flowered earlier than those at 15 $^{\circ}C$ and field conditions, but did not flower at 30 $^{\circ}C$. Seed characters were better in Panax ginseng than in Panax quinquefolium and the amount of seeds showed the highly significant positive correlation coefficient with peduncle length in both Panax ginseng and Panax quinquefolium.

• Korean Journal of Plant Tissue Culture
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• v.27 no.5
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• pp.411-413
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• 2000

The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid posmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. It has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nicotiana tabacum we show that expression of small heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.

• Plant Resources
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• v.1 no.2
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• pp.109-112
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• 1998

Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.212-216
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• 1998

Capsicum pepper selections fro PI163192, PI241670, PI244670, PI271322, PI308787, PI322719, and PI369994 were confirmed to be non-hypersensitively resistant to race 3 of Xanthomonas campestris pv. vesicatoria. A resistant cultivar,‘SR’, was shown to be hypersensitive. Four Koran local cultivars, a cytoplasmic male sterile line (A-line) and its maintainer (B-line) were highly susceptible. The resistant selections and cultivars were crossed with a male sterile A-line (Smsms) and fertility of their F1 hybrids was examined by observing he pollen production, testing pollen germination, and quantifying the amount of pollen produced per anther to identify the genotype interacting with the male sterile cytoplasm. The seven resistant PI selections turned out to be restorers (N(S)MsMs) and‘SR’to be a maintainer (Nmsms).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.117-119
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• 1970

The anthers of Yellow Special A, a leading tobacco variety, were cultured on the modified RM-1964 medium. Approximately three weeks after culture, plantlets emerged out of the anthers, and they reached blooming stage in six months. 24 univalent chromosomes were most frequently observed at M1 of PMC. Number of bivalent was 0.8 per PMC.

• Journal of Microbiology
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• v.34 no.1
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• pp.38-39
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• 1996

The internal regions of nuclear small subunit rRNA from 6 plaeurotus species and 5 Pleurotus ostreatus strains were amplified by PCR and sequenced. The DNA sequences of 8 Pleurotus strains (P. ostreatus NFFA2, NFFA4501, NFFA4001, KFFA4001, KFCC11635, P florida, P. florida, P. sajor-cuju, P. pulmonarius, and P. spodoleucus) were idential, but P. cornucopiae differed from them in two bases out of 605 bases. However, p[hylogenetic analysis of the sequences by DNA-distance matrix and UPGMA methods showed that P. ostreatus NFFA2m1 and NFFA2m2, known as mutants of P. ostreatus NFFA2, belonged to anther group of Basidiomycotina, which is close to the genus Auricularia. The difference of the SSU rDNA sequences of P. cornucopiae from other Pleurotus species tested corresponds to the difference of mitochondrial plasmid type present in Pleurotus species as observed by Kim et al. (1993, Korean J. Microbiol. 31, 141-147).

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.210-216
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• 1998

Taxonomic studies in external morphology, principal component analysis and cluster analysis were conducted to understand the intraspecific relationship among three habitats (Jirisan, Odaesan and Ulleungdo) of allium voctorialis var. platyphyllum. External morphology such as bulb color, leaf blade length and width, petiole length, total leaf length, peduncle length, perianth lobe length and width, length of anther and filament were useful characters for identification of poplations in three habitats. The results obtained based on the principal component (Pc) analysis of treated 72 OTUs(included outgroup) were divided into three groups by the PC 1 ,2,3 and the sums of contributions for the total variance were 84.1%(PC1 51.0%, PC2 24.9% and PC3 8.2%, respectively). In cluster analysis by the UPGMA and Ward's methods , there was similarities in the compostion of clustered taxa, and only Ulleungdo population was distinctly identified from population of other two habitats.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.115-122
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• 1993

KB103 was developed from a cross between Burley 21 and Va 528 in 1983. It was developed by haploid method derived from anther culture method. It has moderate resistance to black shank(Phytophhorn nicotianae Breda de Haan Var. nictiana Waterhouse). KB103 produced good yields and quality in 1988-1990. It flowered about four or five days later than standard variety Burley 21, and plant height, leaf width, leaves per plants were similar to those of Burley 21. In maturing and curing properties as well as in chemical constituents of cured leaves, KB 103 was very similar to that of Burley 21. Regional farm test with the breeding line, KB 8304- ADH 65 named as KB 103 in 1986, was performed for 4 crop years from 1987 at 29 locations. It was released on burley tobacco area in 1992.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.137-144
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• 1993

The present study was conducted to compare the efficiency of individual plant selection for resistance to bacterial wilt with 30 dihaploid lines derived by anther culture and Nicotiana africana method in Fl and F2 generation from a cross between Bright Yellow 4 (BY4) and NC95. F2 dihaploid lines were selected from bacterial wilt disease resistant plants screened under the naturally infested filed conditions. The populations of FB - ADH and FB MDH derived from F2 individual plants with bacterial wilt resistance showed higher resistance to the disease than the populations of Fl - ADH and Fl - MDH, respectively, and no difference for the disease resistance appeared by the haploid deriving method within a generation.

• Journal of Ocean Engineering and Technology
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• v.14 no.4
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• pp.30-34
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• 2000

The concretes cast in the sea water would be likely to be rich mix and mass concrete. Therefore it is important to check out the hydration heat of concrete and to reduce it to prevent the concrete from processing the temperature crack. Recently the antiwashout agent is used on underwater concrete for preventing from the segregation of concrete in the water. The experimental studies were done for the combined cement replaced by fly ash 30%unit weight of binder to study on the characteristics of hydration heat of antiwashout underwater concrete, and its characteristic was discussed by comparing on cast in sea water with anther one in air. The present paper showed that the hydration heat concrete replaced by 30%of fly ash was more significantly reduced than the normal concrete. The hydration heat of antiwashout underwater concrete was highter than that of normal concrete, but it was reduced lower than the normal concrete by adding fly ash.