• 대한수의학회지
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• 제50권3호
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• pp.197-203
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• 2010

Pesticides are useful to eliminate harmful insects and grow crops however, misuse and abuse of pesticides may cause a death of wild birds, livestock, and companion animals. We analyzed residual pesticides in the ingesta and tissues from the dead wild birds, livestock, and companion animals which were suspected pesticide poisoning based on the diagnosis of the Animal Disease Diagnostic Center of National Veterinary Research and Quarantine Service (NVRQS). The samples were primarily brought to NVRQS from local communities and veterinary diagnostic laboratories. The 231 suspicious samples of pesticide poisoning based on the necropsy were analyzed by GC/NPD, GC/FPD, GC/ECD, or GC/MSD in 2008 and 2009. Pesticides were identified from the 55 samples of total 143 samples analyzed in 2008 and from the 34 samples of total 88 samples analyzed in 2009. The pesticide identification rates in 2008 were higher than the rates in 2009. It may have relevance to the increased samples with the outbreak of avian influenza in 2008 in Korea. The concentrations of pesticides found in the dead animals varied and exceeded the respective $LD_{50}$ of pesticides in many cases. Monochrotophos, phosphamidon, and methomyl were the most common pesticides found and those pesticides are high rank sales in Korea. The cause of pesticide poisoning in animal is assuming a misuse and an abuse of commonly used pesticides in Korea.

• 한국환경보건학회지
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• 제27권4호
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• pp.115-122
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• 2001

The purpose of this study was to investigate the metallothionein of acute cadmium poisoning mice as a Cadmium index. Forty male ICR mice were injected with cadmium chloride solution from 1/8LD50 to 1/2LD50 dose. At 24 hours after exposed Cd, I examined Cd and metallothionein (MT) intissues(liver and kidney) and fluids(whole blood and urine) and also measured low molecular proteins, N-acetyl-D-glucosaminidase(NAG) and 2- microglobuline (2-MG) in urine. The concentration of Cd and MT of liver kidney whole blood and urine were increased with dose dependent manner. Urinary Cd and urinary MT and very good significance (p<0.01) and urinary MT had good significance with kidney Cd and NAG but not 2-MG Conclusionally MT in urine was very correlated with kidney Cd and urine Cd, So MT maybe useful as a Cd poisoning index.

• 대한수의학회지
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• 제30권1호
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• pp.85-91
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• 1990

This study was designed in order to investigate the effect of copper sulfate to the ultrastructural changes of the hepatocytes in Sprague Dawley rats. The animals were administered with copper sulfate (10mg/kg B.W.), which was dissolved in normal saline. The solution was injected into abdominal cavity every day. The animals were sacrificed at the 6th, 12th, and 24th day from the beginning of administration. The specimens obtained from the liver were observed with electron microscope and significant changes were as follows. 1. A prominent dilatation and disruption of the cisternae of rough endoplasmic reticulum were recognized. Also, the detachment of membrane bound ribosomes was shown. 2. The proliferation of smooth endoplasmic reticulum and the depletion of glycogen particles were noted. 3. The increase of primary lysosomes and autophagic vacuoles was obserbed. 4. The dilatation of mitochondrial cristae was obserbed. And it was irregulary scattered in the stroma of mitochondria. 5. The atrophy of microvilli in the bile canaliculi and space of Disse was prominent. 6. Membrane of hepatocytes was damaged and significant hydrophic degeneration was obserbed in the perisinusoidal regions. 7. The damage of Fat-storing cells was more significant than that of hepatocytes.

• 한국약용작물학회지
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• 제14권3호
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• pp.139-142
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• 2006

항 식중독균성 수건 및 행주를 개발하기 위하여 이질풀 추출액 1%로 50-60에서 60분간 염색한 면직물의 염색성과 항균성을 조사하였다. 면직물의 표면색은 무매염, 명반 매염, 수산화칼슘 매염시는 그레이-옐로우로 황색계열을 나타냈으며, 황산구리 매염시는 그레이-브라운, 황산제1철 매염시는 그레이드-그린으로 나타났다. 이질풀 추출액으로 염색한 면직물의 hue 값은 20회 세탁까지는 Y계열을 나타내었으며, 30회와 40회 세탁시는 YR계열을 나타내었다. 이질풀 추출액으로 염색한 면직물은 황색포도상구균, 대장균 및 살모넬라균에 대해 무세탁시 모두 99.99%의 항균성을 보였다 세탁 30회 후의 항균성은 52.91%이상을 나타내었다.

• The Korean Journal of Physiology
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• 제2권2호
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• pp.93-99
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• 1968

The Present study attempted to analyze the fate of CO diffused into the circulating blood through the alveoli. Dogs were induced to CO poisoning by rebreathing CO gas mixture contained in Krog's spirometer, by closed circuit method, for 60 minutes. The spirometer was filled initially with 282 ml of CO and 20 liters of air and oxygen, so the composition of gases were arranged as 1.4% in CO and 50% in $O_2$ at the begining of the rebreathing. Oxygen was added corresponding to the utilization of $O_2$ by the animal in proceeding of the experiment. At 60th minutes of CO rebreathing, the concentration of CO in arterial blood and in mixed venous blood were analysed and compared with each other after the CO contents were corrected with the hematocrit measured in the arterial and mixed venous blood. The distribution of CO gas to other tissues was estimated by the analysis of CO diffused into the cystic bile and into the peritoneal gas pocket which was formed by injection of 300 ml air into the peritoneal cavity prior to the CO gas rebreathing. The blood volume was measured by dilution method using $^{51}Chromium$ tagged red cells. CO amount vanished in the animal body was calculated by subtraction of total CO content in blood stream and the CO remained in closed circuit breathing system from the CO amount given to the breathing system at the begining of the experiment. Results obtained are summarized as follows: 1. The content of CO corrected by the hematocrit value was slightly less in mixed venous blood than in arterial blood. The amount of CO diffused into the cystic bile and into the peritoneal cavity was averaged to 0.1% and 0.4% of the CO amount in 100 ml of blood, respectively. 2. For 60 minutes of CO rebreathing, CO-hemoglobin saturation reached about 77% at the 60th minutes, CO amount vanished in the experimental animal averaged 36.1 ml/dog/hr., or 21% of the total CO volume in the blood stream. The average vanishing rate of CO during 60 minutes of CO rebreathing per kg of body weight was 2.71 ml/hr. Production of CO measured in ten dogs under hypoxic condition averaged 0.023 ml/kg/hr. The major part of the CO vanished in the dogs seemed to be oxidized to $CO_2$ by various tissues of the animal. The conclusion might be delivered as such oxidation of CO to $CO_2$ by animal tissues can play a role in part of the process of recovery and protection of animal from CO-poisoning.

• 한국동물위생학회지
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• 제41권2호
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• pp.119-123
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• 2018

There has recently been a growing demand for pathodiagnosis to determine the cause of death in cats. We retrospectively analyzed the diseases diagnosed in cats that were submitted to Animal and Plant Quarantine Agency (APQA) in 2015~2017. Overall diagnostic rate in feline samples was 85.2% (n=104/122). Among diagnosed cases, infectious diseases (n=63) were responsible for most of the feline diseases and feline panleukopenia (n=29) were most prevalent. Highly pathogenic avian influenza (HPAI) H5N6 was first diagnosed in cats at the end of December 2016 in the HPAI outbreaks. One case in 2015, 4 cases in 2016, and 14 cases in 2017 were associated with animal abuse, such as trauma and poisoning. These results suggest that suitable vaccination of feline infectious diseases, monitoring of the susceptible domestic animals during HPAI outbreaks, and interest on veterinary forensics to prevent and determine animal abuse are needed.

• 한국임상수의학회지
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• 제41권3호
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• pp.189-194
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• 2024

Cinereous vulture (Aegypius monachus) is a large raptor that belongs to the family Accipitridae in the order Falconiformes. It is a migratory bird that returns to Mongolia after wintering in the Korean Peninsula. In February 2015, a cinereous vulture was rescued from Asan-si, Chungcheongnam-do Province as it could not fly. It was transferred to the Chungnam Wild Animal Rescue Center in Korea. It was found to have a closed fracture on the right radius through radiographic examination, a basic procedure. Surgery was performed at the fracture site using a shuttle pin and wire. After successful physical therapy and rehabilitation, the cinereous vulture was released in October 2015. A metal ring (150-00713) and wing tags (G/BL 3) were attached to the bird before its release for subsequent identification. Two years and 4 months later, in March 2018, the cinereous vulture was rescued and brought back to the center. It was diagnosed with secondary pesticide poisoning. After receiving treatment, a radiography test revealed no specific findings related to the previous fracture surgery. Considering that migratory birds travel long distances, it was confirmed that the treatment for reducing the fracture in the radius using a shuttle pin and a cerclage wire was suitable.

• 한국임상수의학회지
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• 제26권4호
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• pp.344-347
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• 2009

2-4세령의 면양 4두와 1-3세령의 한국재래산양 5두가 정원수를 가지치기 한 후 던져준 철쭉 (Rhododendron schlippenbachii)을 섭취하고 난 후 다음날 식욕감퇴, 무기력, 욕지기, 침흘림, 구토, 호흡곤란, 비틀거림, 서맥 등의 중독증상을 나타내어 철쭉중독으로 진단한 후 atropine과 carbo-pulbit를 처치하였다. 처치 후 8시간째에 1두의 한국재래산양이 폐사되어 부검을 실시한 결과 위 내에서 다량의 철쭉잎이 관찰되었으며, 나머지 면양과 한국재래산양은 3일 이내에 모두 회복되었다.

• 대한의생명과학회지
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• 제11권3호
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• pp.295-299
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• 2005

Staphylococcus aureus is an important animal and human pathogen implicated in a variety of disease including food-poisoning caused by staphyloccal enterotoxins (SEs). In order to investigate the difference in genomic types and to monitor the transmission of S. aureus isolates, a total of 25 S. aureus isolates from different sources were determined for their genotypic characteristics by pulsed-field gel electrophoresis (PFGE) in addition to their ability to enterotoxin production and antibiotic resistance patterns in this study. All the isolates were susceptible to amikacin, and the resistance pattern to ampicillin and penicillin were most common among 14 different patterns. Eleven of 24 isolates produced one of three SEs, SEA, SEC or SED. Sixteen representative PFGE patterns were obtained by Smal restriction fragments of S. aureus isolates. Analysis of dendrogram based on PFGE band patterns suggested that food-poisoning outbreaks be caused by the diverse sources of food, of which their raw materials were infected with S. aureus. Also, it could be concluded that PFGE was a powerful tool for epidemiological tracing of infection source for food-initiated outbreaks.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.291-294
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• 2006

Clostridium perfringens strains were isolated from swine diarrhea in Korea. Three out of nineteen (15.8%) isolates of C. perfringens were found to be enterotoxigenic by PCR analysis. PCR-based genotyping of the three enterotoxigenic isolates of C. perfringens revealed that they were types A, C and D, respectively. These results suggest that various types of enterotoxigenic C. perfringens can cause swine diarrhea, and that the presence of enterotoxigenic type A strain, known to be strongly associated with food poisoning, may cause public health problem in Korea.