• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.3
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• pp.219-226
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• 1986

To confirm the application of a newer in vitro assays to determining the nutritional value of marine crustaceans (mainly shrimps and crabs), which have been considered to be highly nutritive depending on their levels of the essential amino acids and digestibility, their C-PERs and DC-PERs were determined and studied the factors influencing their in vitro results. Four species of seawater shrimps and 2 species of seawater crabs were used in this experiment. The in vitro digestibilities showed $83{\sim}86\%$ for raw shrimps and the trypsin indigestibile substrate content (TIS) was ranged from 1.32 to 3.33 mg/g solid expressed quantitatively as mg of purified soybean trypsin inhibitor. The smaller size of shrimps revealed a greater in vitro digestibility and a lower contents of TIS. It was noted that the in vitro digestibility of raw blue crab meat was around $85\%$ while boiled tenner crab meat showed $86\%$ or above, and the leg meat had the greatest in vitro digestibility in the various parts of crab meats. The poor in vitro digestibilities for shrimp's and crab's meat, compared with that of the other seafoods as noted in previous reports, suggest that the drop in pH, due to the change in their freshness during harvesting and frozen storage, resulted in underestimating their digestibilities using four-enzyme digestion technique. The lysine contents in all samples were higher than that of ANRC casein but they contained a slightly lower sulfur-containing amino acids than those in ANRC casein. But the other EAA, such as valine, tyrosine and phenylalanine, were found to be a half as little as that in casein and played a key-factor in calculation of C-PER or DC-PER. It was observed that the value of C-PER and DC-PER for all samples ranged from 2.1 to 2.4, and the predicted digestibilities showed $90\%$ or above in all samples. It was a different results from the fact that the animal proteins bear a higher values and predicted digestibilities than those of C-PER values. The lack of correlation between C-PER and DC-PER values is attributable to the fact that the lower content of valine, tyrosine and phenylalanine, and drop in pH owing to the changes of freshness in marine crustacea proteins. Therefore, if a newer in vitro digestion technique-which are taken into account the pH drop before digestion, TIS content and released free amino acids and/or peptides-developed, C-PER assays can provide more advantages in assessing the protein nutritional value of marine crustacea than any other in vitro assays.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.3
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• pp.160-167
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• 2006

Harpacticoid copepod Tigriopus japonicus is widely distributed in coastal waters of Korea and plays important role in marine trophic structure as a first consumer. In this study, a series of experiment were conducted to test the potential of the species as a standard test species for marine bioassay. Tolerance on salinity and pH, sensitivity on the reference materials(copper sulfate and cadmium chloride) and response on the ocean dumping materials(waste sludge) we re tested to identify if the species satisfy the basic criteria as standard species for marine bioassay. The nauplius of the species($100{\sim}200{\mu}m$) showed wide tolerance on salinity with >90.0% survival rates exposed to $5.0{\sim}35.0psu$ for 48 h. Wide adaptability on pH's were also observed from 6.3 to 8.2 with >90.0% survival rates during the test. $LC_{50}$ values for copper sulfate and cadmium chloride were $3.6{\pm}0.7ppm,\;1.7{\pm}0.8ppm$, respectively. The variations in mortality between replicates were less than 10.0%. Comparison of $LC_{50}$ values indicated that T. japonicus nauplius was lower sensitive to copper sulfate than the most marine crustaceans included copepods, however, the sensitivity of test animal to cadmium chloride higher than the adults of copepod T. japonicus, Paracalanus parvus, and marine rotifer Brachinonus plicatilis. There were significant concentration-response relationship in the mortality of T. japonicus nauplius using the elutriates of three ocean dumping materials(industrial waste sludge). 48 h $LC_{50}$ values we re $31.1{\pm}1.1%$ for the elutriate of sludge from leather processing company and $54.4{\pm}15.1%$ for that of dye production company. Based on the above experimental results, bioassay using benthic harpacticoid T. japonicus nauplius must be a good estimation tool for marine ecotoxicological assessment of waste or chemicals. Wide tolerance on the salinity and pH, and significant linear relationship between concentration and response(mortality) supported the high potential of the species as a standard test species.

• Journal of the Korea Organic Resources Recycling Association
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• v.2 no.2
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• pp.65-75
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• 1994

The aim of this experiment was to investigate the effects of applying time of cattle slurry and fertilizing added mineral nitrogen on dry matter yield of orchardgrass and also to estimate proper levels of fertilizing nitrogen when idled arable land rapidly increased these days, which was used for the production of Orchardgrass. The results were as follows. 1. The annual yields of dry matter were produced 5.62~6.67 ton per ha when cattle slurry applied at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in different dressing times. Those were higher 0.60~1.65 ton or 0.16 ton per ha than the yields from non-fertilizing or phosphrous and potassium fertilizing. 2. Relative yields of annual dry matter from mineral nitrogen fertilization of 120kg per ha were 142~146% in comparsion with control plots(cattle slurry plot or phosphrous and potassium plot). On the other hand, those of cattle slurry application were 112~133% in comparion with non-fertilizing plot. 3. The efficiencies of nitrogen on dry matter yield(kg DM/kg N) were 5.0~13.8 DM kg/N kg when cattle slurry applied to Orchardgrass at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha, and were 21.3~23.1kg DM/kg N at the same level fertilization of mineral nitrogen. 4. On the condition of this study, mineral nitrogen were fertilized after applying cattle slurry at rates of $30m^3$(average mineral fertilizer equivalent to 120kg) per ha in order to minimize chemical fertilizer and increase the potential production of Orchardgrass. At this situation, marginal dry matter yields of 8.48~8.63 ton per ha were obtained as the level of economic even point goint when mineral nitrogen fertilization is between 119.4 and 133.3kg per ha. It was possible to obtain the maximal dry matter yield(8.98 tons/ha) with fertilization of 202.8kg N/ha.

• The Korean Journal of Pesticide Science
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• v.16 no.3
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• pp.261-266
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• 2012

It is common to use many experiment animals to evaluate the toxicity of chemicals including pesticides. For protecting animal, the concepts of 3R (Reduction, Replacement, Refinement) were introduced and in vitro alternatives methods actively have been developed all over the world. Many experimental animals for toxicological tests have been used, so that it is important to establish the alternative methods. In this study, the alternative method using reconstituted human skin model (Keraskin$^{TM}$) was conducted for classification of skin irritation on pesticides. Sixteen formulations selected on the basis of the degree of irritation were treated by Keraskin$^{TM}$ test. The percent of cell viability was measured into the culture medium collected after treatment of the pesticides for 24-72 hrs. The skin irritations of formulations were evaluated by the cell viability. In this study, The 4 formulations with mild irritation in rabbits were evaluated as nonirritant, the 6 formulations with moderate and severe irritation were evaluated as irritant in human skin model test. We suggest that the alternative test using Keraskin$^{TM}$ model could be used as toxicity evaluation for primary irritation index (P.I.I.) score of greater than or equal to 2.1 of pesticides. The further studies should be required to apply for hazardous assessment of pesticides on alternative skin irritation methods because of the interindividual variability of the sensitivity of skin irritation on pesticides.

• Journal of Chest Surgery
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• v.43 no.1
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• pp.11-19
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• 2010

Background: The commercially used vascular xenografts have some problems such as calcification, fibrosis and tissue degeneration that are associated with inflammatory and immunologic reactions. We compared two methods of xenograft preservation (fresh cryopreservation versus acellularized cryopreservation) of goat aorta. Material and Method: Aortic valved xenografts were harvested from adult pigs, and these were preserved using fresh cryopreservation (FC group, n=4) or acellularized crypreservation (AC group, n=4). These xenografts were implanted into adult goats. There were 2 short-term survivors (less than 100 days) and 2 long-term survivors in each group. These xenografts were explanted and they underwent microscopic examination. Result: The goats survived 31, 40, 107 and 411 days in the FC group and the other goats survived 5, 40, 363 and 636 days in the AC group. All the short-term survivors in each group expired because of rupture at the proximal anastomosis site. Marked neutrophil infiltration was observed in the FC group FC and lymphocytes were observed in the AC group. There were no differences in the occurrence of calcification, fibrosis and thrombosis among the groups. Conclusion: Some goats survived more than 100 days after the xenograft implantation irrespective of the methods of preservation. Because severe tissue degeneration developed in both groups, we think these methods are not appropriate for xenograft preservation of aorta. It was worth a preliminary trial for improving the preservation method or to modify the processing of xenografts.

• The Korean Journal of Nuclear Medicine
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• v.32 no.3
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• pp.298-304
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• 1998

Purpose: We evaluated the usefulness of Re-188 sulfur colloid for radiation synovectomy and therapy of intraperitoneal metastasis. Materials and Methods: We investigated the labeling efficiency of Re-188 sulfur colloid on various conditions. The stability of Re-188 sulfur colloid was observed at room temperature for 24 h and in human serum and synovial fluid for 72 h. The particle size distribution of Re-188 sulfur colloid was measured by filtering with various pore size filters. Animal experiment was performed in mice and rabbits. Results: The labeling efficiency of Re-188 sulfur colloid was $64.5{\pm}5.8%$ (n=5) at the conditions of sodium thiosulfate 40 mg, EDTA $Na_2.2H_2O$ 0.8 mg, $KReO_4$ 0.8 mg at pH 1. After purification, the radiochemical purity was higher than 99%. The stability of Re-188 sulfur colloid was high (>99%) at room temperature for 24 h and in human serum and synovial fluid for 72 h. The particle size distribution of Re-188 sulfur colloid was 0.3% ($<1{\mu}m$), 11.2% ($1{\sim}5{\mu}m$), 25.8% ($5{\sim}10{\mu}m$) and 52.8% ($>10{\mu}m$). In mice, 1 h postinjection of Re-188 sulfur colloid into tail vein, uptakes in lung, liver and muscle were $37.30{\pm}5.36$, $32.33{\pm}1.79$, $6.60{\pm}0.02%$ ID/organ respectively. After i.p. injection in mice, the uptakes of extraperitonial organs of Re-188 sulfur colloid at 1 and 24 h were $0.1{\pm}0.1$, $0.4{\pm}0.1%$ ID/organ, and the excretions through urine and feces (${\sim}70 h$) were low ($2.68{\pm}0.80$, $0.95{\pm}0.17%$). When Re-188 sulfur colloid was injected to synovial space of rabbit, the uptake in other organs except knee was very low. Conclusion: Re-188 sulfur colloid showed high labeling efficiency, stability and potency for clinical use.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.57-62
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• 2015

"Nokyang" (Hordeum vulgare L.), a new whole-crop silage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA, Korea in 2012. It was derived from combining "Nagyoung" and breeding line "SB77368-B-145". Among the combinations made in 2001, a promising line, "SB01T2017", showed good characteristics in terms of potential forage yield in the yield trial at Iksan from 2007 to 2009. In 2009, the promising line was designated as "Iksan457" and placed in regional yield trials at six locations around Korea for three years from 2010 to 2012, and was released under the name of "Nokyang." It has the growth habit of a group III creeping plant type, with a light-green leaf and common spike. Its average heading and maturing dates were on May 6 and June 4, respectively, which were later than cultivar "Youngyang." "Nokyang" also showed strong winter hardiness, and a resistance to shattering and barley yellow mosaic virus (BaYMV) similar to those of the check cultivar. The best thing among the traits of one is a new good quality with the plant green at the latter growing period. The average forage dry matter (DM) yields in the regional yield trial were about 12.8 and 11.5 MT $ha^{-1}$ in upland and paddy field, respectively, which were higher than those of the check cultivar, by 7% and 2%, respectively. This cultivar would be suitable for an area of the Korean peninsula where the daily minimum temperature in January is above $-8^{\circ}C$.

• Journal of Life Science
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• v.19 no.1
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• pp.65-74
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• 2009

The objective of this study was to examine the effects of steam flaking treatment of corn grains imported from USA and India on in vitro gas production, microbial growth and contents of aflatoxin $B_1$ and ochratoxin A. Each treatment was composed of total 4 treatments including (1) USCW (US com-whole type), (2) USCF (US corn-flaked type), (3) IDCW (India corn-whole type) and (4) IDCF (India orn-flaked type) with 4 replications $\times$ 6 incubation times (3, 6, 9, 12, 18 and 24 hr). Mycotoxin (aflatoxin $B_1$ & ochratoxin A) contents in test corns tended to increase gradually with increasing logistics periods from the harbor, hopper, silo to processing line. The contents of aflatoxin $B_1$ in India corn (IDCW) and US corn (USCW) were 11.71 and 1.78 ppb, respectively when measured at the hopper. After steam flaking, both contents of aflatoxin $B_1$ in USCW and IDCW were 0.00 ppb. It means that Aspergillus flavus could be decreased by steam flaking. However, this trend was not observed in ochratoxin content. The gas production rate of USA corns (USCW & USCF) was significantly (p<0.05) higher than India corns (IDCW & IDCF), and that of steam flaked corns (USCF & IDCF) was higher $1.5{\sim}2%$ than whole corns (USCW & IDCW) after 3 hr incubation in in vitro experiment. pH value was optimally maintained for microbial growth during whole incubation times with the value of 6.05 to 6.54, and was not significantly different between treatments, but USCF was somewhat lower than other treatments. pH value decreased following 12 hr of incubation but gas production increased rapidly during the same period. In addition, in vitro microbial growth rates also increased with up to 18 hr of incubation period, thereafter experienced a decrease with extended incubation time. In conclusion, US corn was superior to India corn by origin based on the results of in vitro and mycotoxin contents. And steam flaking process of imported corns tended to decrease mycotoxin contents such as aflatoxin $B_1$ and ochratoxin A as well as improve in vitro gas production and microbial growth rates.

• Korean Journal of Weed Science
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• v.3 no.2
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• pp.223-245
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• 1983

Nationwide weed survey of paddy rice field conducted in 1981 and 1971 was compared and determined major dominant weed community types distributed by province. Based on the similarity coefficients between 1971 and 1981 the floristic composition based on the degree of dominance was greatly dissimilar while kind of weed flora were not much differed. Degree of dominance were concentrated to perennial weeds in 1981 while these were at animal weeds in 1971. The moat important 10 weed species and their dominance in 1981 were Monochoria vaginalis Presl.(22.2%), Sagittaria pygmaea Miquel(17.5%), S. trifolia L.(9.0%), Poramogeton distinctus Benn.(9.0%), Cyperus serotinus Rottb.(8.5%), Rotala indica Koehne(6.0%), Aneilema japonica Kunth(4.4%), Lindernia procumbens Philcox(3.9%), Eleocharis kuroguwai Ohwi(3.4%) and Ludwigia proarrara Roxb(3.0%), respectively while these for 1971 were R. indica(34.5%), Eleocharia acicularis Roem, et Schult (11.9%), M. vaginalis(11.1%), Cyperua difformis L.(8.7%), Echinochloa crus-galli Beauv.(6.9%), L. procumbens(3.3%), P. distinctus(3.1%), A. Japonica (2.4%), E. kuroguwai(1.8%) and P. hydropiper(1.8%), respectively. Weed occurrence was also closely related with soil type, cropping pattern and cultural practices. Particularly, the occurrence of P. distincrus was negatively correlated with the degree of land utilization. Weed community types dafined by two-dimensional ordination analysis were 11 for Jeonnam province, 9 for Gyeoaggi, Gangweon, Chungbuk and Gyeongnam provinces, 8 for Jeonbuk and Gyeongbuk provinces, 7 for Chungnam province and 4 for Jeju province, respectively.

• Journal of Chest Surgery
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• v.35 no.3
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• pp.171-176
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• 2002

Background: Blood-foreign interaction cause activation of coagulation and inflammatory process that may lead to multiorgan dysfunction and determine the surgical outcomes. Of the methods for assessing the biocompatibility, the platelet adhesion study is considered as the most valuable evaluation step in blood-foreign interaction. As the most studies have used in-vitro or ex-vivo conditions, we have developed a technique of quantification for platelet adhesion on the blood contact surface by using in-vivo injection of radioactive platelets. Material and Method: A coupled bypass circuit was designed to connect the proximal and descending thoracic aorta in 6 piglets(20∼25 Kg). One side of the circuit tube was consisted of a heparin coated PVC tube(10mm in ID, n=6, Experimental group), and the other, a non-heparin coated PVC tube(10mm in ID, n=6, Control group). After cannulation, the blood was circulated through the circuit for 2 hours. Platelet concentrate was prepared from homologous pig blood 24 hours before the experiment. The platelet concentrate was incubated with Tc-99m-HMPAO for 30 min and then centrifuged for 10 min. The supernatant was discarded and the radio-labeling efficacy was measured. The radio-labeled platelet concentrate was mixed with the autologous plasma to make the volume 5 ml, and the mixture was injected intravenously into the experimental animal. After 2 hour circulation, 5 pieces of the specimen(10mm in length each) were obtained from each PVC tube. The radioisotopes were counted with a gamma counter(Cobra ll, Packard, USA), and the ratio of radioisotope count was compared between the control and experimental group. Result: The radioisotope count number was 537.3221.1 Ci/min in the control group and 311.1 184.5 Ci/min in the experimental group(p=0.0104). The ratio between the groups was 1 to 0.58 (p=0.004). Conclusion: In vivo quantification using technetium-99m-HMPAO labeled platelets is simple and reproducible in evaluating platelet adhesion on a foreign surface. We suggest this technique to be a useful tool for blood compatibility test.