• Mass Spectrometry Letters
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• 제13권4호
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• pp.133-138
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• 2022

Taurine is a type of sulfur-containing amino acid having a sulfate functional group, that is biosynthesized from cysteine. It is mainly distributed in high concentrations in animal tissues and is known to have various effects such as osmotic pressure control, calcium control, anti-inflammatory, antioxidant, and hepatocellular protection. Also, taurine deficiency causes a variety of symptoms, including visual impairment. In particular, in the case of cats, taurine is not biosynthesized and must be supplied through food, so it is classified as an essential amino acid. In this study, an analysis method using mass spectrometry was developed instead of the commonly used derivatization method to quickly, environmentally, and precisely analyze taurine in various animal feeds. The developed analytical method showed good linearity (R² > 0.99), accuracy (81.97-105.78%), and precision (0.07-12.37%). In addition, the developed method was further verified through quantitative comparison with the derivatization method. This developed method was used in the determination of taurine in 20 animal feed samples obtained from South Korea. The levels of taurine found ranged from 81.53 to 6,743.53 mg/kg. The developed analysis method will be used for the detection and quantification of taurine in domestic feed.

• Asian-Australasian Journal of Animal Sciences
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• 제9권6호
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• pp.643-646
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• 1996

Experiments were conducted to determine potassium (K) requirement of mule ducklings. One-day-old ducklings with equal number of both sexes were fed diets containing graded levels of K for three weeks. In experiment 1, corn-soybean meal diet (CP 18.7%, ME 2,890 kcal/kg, K 0.80%) was used. The addition of K (0.00, 0.10, 0.20, 0.30, 0.40%) to the diet by potassium carbonate resulted in a decrease in weight gain and feed efficiency with the group fed on basal diet having the best performance. This means that the existing K in the basal diet is adequate for the growth of ducklings. In experiment 2, low K corn-isolated soy protein diet (K, 0.19%) was used. The supplementation of K (0.00, 0.16, 0.32, 0.48, 0.64%) resulted in an increase in weight gain and feed efficiency with the control group having the lowest performance. The minimum K requirement was found to be 0.49% for both maximum growth and best feed efficiency as determined by using bent-stick model.

• Asian-Australasian Journal of Animal Sciences
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• 제25권12호
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• pp.1691-1700
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• 2012

The experiment studied the effect of harvesting frequencies and varieties on yield, chemical composition and hydrogen cyanide content in cassava foliage. Foliage from three cassava varieties, K94 (very bitter), K98-7 (medium bitter) and a local (sweet), were harvested in three different cutting cycles, at 3, 6 and 9 months; 6 and 9 months and 9 months after planting, in a 2-yr experiment carried out in Hanoi, Vietnam. Increasing the harvesting frequency increased dry matter (DM) and crude protein (CP) production in cassava foliage. The K94 variety produced higher foliage yields than the other two varieties. Dry matter, neutral detergent fibre (NDF), acid detergent fibre (ADF) and total tannin content increased with months to the first harvest, whereas CP content decreased. Hydrogen cyanide (HCN) content was lower at the first harvest than at later harvests for all cutting cycles. At subsequent harvests the content of total tannins tended to decline, while HCN content increased (p<0.05). Chemical composition differed somewhat across varieties except for total tannins and ash. Dry matter, NDF, ADF and total tannins were higher in fully matured leaves, while CP and HCN were lower in developing leaves.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.1-6
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• 2001

Additive and dominance genetic variances were estimated for purebred Landrace selected with line breeding from 1989 to 1995 at Miyazaki Livestock Experiment Station, Kawaminami Branch. Ten body measurements, two reproductive traits and fifteen carcass traits were analyzed with single-trait mixed model analysis. The estimates of narrow-sense heritabilities by additive model were in the range of 0.07 to 0.46 for body measurements, 0.05 to 0.14 for reproductive traits, and 0.05 to 0.68 for carcass traits. The additive model tended to slightly overestimate the narrow-sense heritabilities as compared to the additive and dominance model. The proportion of the dominance variance to total genetic variance ranged from 0.11 to 0.91 for body measurements, 0.00 to 0.65 for reproductive traits, and 0.00 to 0.86 for carcass traits. Large differences among traits were found in the ratio of dominance to total genetic variance. These results suggested that dominance effect would affect the expression of all ten body measurements, one reproductive trait, and nine carcass traits. It is justified to consider the dominance effects in genetic evaluation of the selected lines for those traits.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1600-1605
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• 2007

This study was undertaken to investigate the effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidant enzymes of weanling pigs. In the experiment, 24 male $Landrance{\times}Yorkshire $weanling pigs were allotted to three groups of 8 animals each. Pigs were fed individually. According to a single factorial arrangement, pigs received diets with 5% of either fresh (group 1 and group 3) or oxidized fish oil (peroxide value was 786.50 meq $O_2/kg$ before inclusion in the diet, group 2). At the beginning of the experiment, pigs in group 3 received an intraperitoneal injection of diquat at 12 mg/kg of body weight. The trial lasted for 26 d. A metabolism test was carried out during the last 4 days of the second week. The results showed that feeding diets containing oxidized fish oil or injection with diquat depressed the growth performance and nutrient digestibilities of weanling pigs, decreased activities of antioxidant enzymes and increased concentration of malondialdehyde in plasma and liver. Intraperitoneal injection of diquat would induce more serious oxidative stress than oral intake of oxidized fish oil in the diet. In conclusion, administration of oxidized fish oil or diquat could induce oxidative stress in weanling pigs, and oxidative stress could depress growth performance and impact anti-oxidative ability of young pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.376-380
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• 1999

The purpose of this experiment was to study the effect of feeding winged bean (Psophocarpus tetragonolobus) oil on egg cholesterol and fatty acid of Japanese quail. Four groups of 10-week-old Japanese quails (n=10 per group) were fed a basal diet (20% CP, 2890 kcal/kg) supplemented with 5% of either animal tallow, soybean oil, corn oil or winged bean oil over an 8-week period. At the end of the experiment, 7 birds in each treatment were killed by decapitation. Blood samples and livers were collected for cholesterol analyses. There were no significant differences in final body weight, feed intake, egg production, egg and yolk weight due to the different oil treatments. Egg and plasma cholesterol levels obtained with the winged bean oil diet were higher than those obtained with the animal tallow or soybean oil diets, but were not significantly different from those obtained with com oil diet. Egg lipid, liver lipid and liver cholesterol content was lower with the winged bean oil diet than with the animal tallow diet. It may be concluded that the winged bean oil diet did not impair laying performance, but increased egg and plasma cholesterol levels compared with soybean oil or animal tallow diets. Winged bean oil diet produced eggs with a higher oleic content and lower linoleic content compared with the soybean or com oil diet.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.57-61
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• 1996

Four castrated Corriedale sheep were used in an experiment to observe the changes in insulin, growth hormone and cortisol in blood plasma following a prolonged infusion of a high rate of somatostatin (SRIF). The animals wee infused with either saline, 25 or $50{\mu}g/kg/h$ of SRIF for 3 hours. Blood samples wee taken every 20 minutes until 1 hour following the end of the SRIF infusion. Both SRIF infusion levels suppressed the release of insulin into plasma to approximately 3.5 mU/l. The SRIF infusions reduced the concentration of growth hormone to barely detectable levels. Following the withdrawal of SRIF there was a massive release of growth hormone. The plasma concentration of growth hormone reached 60 ng/ml within 20 minutes, the length of the growth hormone discharge was in excess of 1 hour. The extent of the discharge of growth hormone following the SRIF infusions was greater than that suppressed by the infusion. The SRIF apparently caused an increase in the plasma concentration of cortisol at the end of the infusion and following is withdrawal. This is possibly associated with some change in the metabolic rate associated with the suppression of insulin or glucagons release. The present experiment demonstrates that a high rate of SRIF infusion can not completely inhibit the release of insulin into the plasma.

• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.881-885
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• 1999

A growth study was conducted for 238 days in twenty crossbred cattle calves to observe the effect of dietary concentrate supplement on blood biochemical changes and serum testosterone levels. The calves were divided into four groups (A, B, C and D) of five animals each. Calves of groups A and B were fed 60% and 30% concentrate, respectively, supplying equal amount of protein along with wheat straw. The calves in group C received 30% concentrate in their diet for 1 to 119 days of experiment and 60% concentrate during 120~238 days of experiment and vice versa in group D. Mean DM and TDN intake were significantly higher in group A than group B, C or D, resulted in higher daily growth rate in the former group. Blood glucose level was significantly higher in group A where as blood urea, hemoglobin, total protein, albumin, and globulin levels remained unchanged among the groups. Serum testosterone level increased with the increasing age of the animals but the level remained same in the animals of group A, B, C and D. A 30% concentrate diet does not have any severe adverse effect on the performance of crossbred cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.15-19
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• 1997

An experiment was conducted to clarify the effects of dietary supplementation of monensin or salinomycin on mineral utilization of growing goats. Six goats weighing 10.54 kg initially were randomly assigned to treatments in a replicated $3{\times}3$ Latin square design. Treatments were a basal diet, basal plus 30 ppm monensin and basal plus 20 ppm salinomycin on a DM basis. The basal diet was a mixture of Italian ryegrass wafer, soybean meal, ground maize and $CaCO_3$ with DM proportions of 50, 13.76, 36 and 0.24%, respectively. Each period lasted for 21 days, and the apparent absorption and retention of minerals were measured during the last 7 days of each period. Salinomycin supplementation improved NDF digestibility and plasma glucose. The apparent absorption and retention of Ca, P, Mg, Na and K were not influenced by the treatments. The concentrations of plasma Ca, P, Mg, Na, K and Cl were similar in all treatments. The ionophore supplementation had no significant effect on acid excretion. The results suggest that 30 ppm monensin or 20 ppm salinomycin supplementation is not effective in improving the utilization of Ca, P, Mg, Na and K in growing goats fed a diet composed of the 50% concentrate.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.183-189
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• 1994

Two experiments were made. In experiment 1, four diets containing 0, 5, 15 and 25% washed seaweed meal were prepared to study the effects of incorporating seaweed meal instead of equivalent amounts of berseem leaf meal in fish feeds on growth performance and feed utilization of common carp. The results showed that average daily gain (ADG), specific growth rate (SGR%), dry matter (DM) and ether extract (EE) of the carcasses were decreased (p<0.05) with the increasing level of seaweed meal in the feeds. Inclusion of 5% seaweed meal in the diet gave the best feed conversion ratio (FCR) among all diets, however, protein productive value (PPV) and energy utilization (EU) were decreased (p<0.05) with increasing level of seaweed meal in the diet. In experiment 2, washed seaweed meal was either steam cooked or sprayed with NaOH (0.5% or 1% NaOH) and incorporated in the diets at the level of 25% instead of equivalent amount of berseem leaf meal. The results showed that steam cooked seaweed gave the best (p<0.05) growth performance, FCR and protein efficiency ratios, PER and PPV, for other treatments in descending order were NaOH treated seaweed, washed seaweed and unwashed seaweed.