• Journal of Plant Biotechnology
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• v.8 no.1
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• pp.27-35
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• 2006

Plantlet regeneration in Asteracantha longifolia(L.) Nees (Acanthaceae), a medicinal herb has been achieved from seedling explants on basal MS medium. Three different seedling explants including node, internode and leaf segments on used. Of these three explant, leaf explants gave better response for both callus mediated organogenesis and direct multiple shoot induction. Number of explants showing differentiation of shout buds was higher on MS media supplemented with BA compared to kinetin. MS medium fortified with BA ($2.0mgl^{-1}$) and NAA ($0.5mgl^{-1}$) was found to be most suitable for both callus mediated organogenesis and elongation of shouts. The elongated shoots were successfully routed on MS medium fortified with NAA or IBA. Among them $0.1mgl^{-1}$ NAA or $0.2mgl^{-1}$ IBA provides better response for rhizogenesis. Regenerated plantlets were successfully established in soil where 85.4% or them developed into morphologically normal and fertile plants. RAPD profiling using four decamer primers confirmed the genetic uniformity of the regenerated plantlets and substantiated the efficacy and suitability of this protocol for in vitro propagation of A. longifolia.

• Journal of Plant Biology
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• v.17 no.3
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• pp.127-136
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• 1974

The effect of potassium metabolism on the soybean leaves was studied with comparison of other elements during the successive growing period. The results were as follows; 1. The percentage of potassium content showed remarkable increase not only in the first compound leaf at a stage which was growing vigorously and producing new leaves, but also in the fifth compound leaf at a stage which was taking a active metabolism of nitrogen and carbohydrate but not producing new leaves. However, the percentage of potassium content was decreased in the second compound leaf than in the first one. Such a result could be regarded as a potassium removal from mature leaves into immature and flowing out from stoma through respiration. During the pod-development the percentage of potassium content in the soybean leaf was decreased. 2. If nitrogen, phosphorus and potassium were added excessively in the nutrient solution, the percentage of potassium content in the soybean leaf had increased. The effects of these elements showed a remakable increase in the excessive plot of nitrogen than in that of phosphorus. At early stage the redtarded effect of phosphorus on the growth of soybean could be covered by potassium, however, at late stage it could not. The growth of soybean plant was much more inhibited by potassium, compared with nitrogen and phosphorus. New leaves could not be produced in the potassium deficient soybean plant after the third compound leaf. The normal growth of soybean plant could not be observed if only one element was excessively added to the culture solution, compared with the deficiency of other two elements.

• Korean Journal of Plant Resources
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• v.23 no.3
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• pp.233-241
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• 2010

In vitro elimination of Sweet potato leaf curl virus (SPLCV) from infected sweet potato is difficult due to low number of virus-free plants obtained from meristem tip culture and long growth period required for the virus detection. In this study, efficient production of the SPLCV-free sweet potato by in vitro therapy coupled with a PCR assay for virus detection was investigated. Infected shoots cultured on Murashige and Skoog medium were treated at three different temperatures for 7 weeks followed by meristem tip culture on the medium with or without ribavirin at 50 mg/L. The regenerated plantlets were tested for virus infection by a PCR assay. The results showed that the both heat- and cold-treatments, and addition of the ribavirin did not have significant effect on efficiency of the virus elimination. The meristem size, however, greatly affected the survival rate. Meristems sized over 0.4 mm survived better than smaller ones (0.2-0.3 mm). The PCR assay was approved to be a rapid, sensitive and reliable for the SPLCV detection in regenerated plantlets. Therefore, combination of cultivating meristem tips sized 0.4-0.5 mm on the medium at $22^{\circ}C$ without ribavirin and detection of SPLCV in the regenerated plantlets by the PCR assay was an efficient system for the SPLCV elimination from infected sweet potato.

• Journal of Plant Biology
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• v.35 no.1
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• pp.9-15
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• 1992

The plantlet was regenerated on MS medium containing BAP (2 mg/I) and 1M (1 mg/I) from leaf discs of pepper after 3 weeks of culture. And then, we investigated the activity of peroxidase and esterase and the pattern of their isozymes from leaf, stem and root in order to observe physiological and biochemical changes on the developemental stage, respectively. The peroxidase was expressed with tissue specificity because peroxidase activity according to the developemental stage of the tissue was not only highest in the leaf of the pepper at 10 days after it germinated but also 2 new bands of its isozyme were found in pI 7.2 and pI 5.2. However, a new pI 3.4 band was found in the leaf and root of the pepper after 14 days of germination, and in the stem was found out pI 5.2 band. As regeneration of leaf dises was progressed, its peroxiase activity was increased about 80% more than that of control after 14 days of culture and new pI 3.2 and 6.5 bands of it isozyme were found. The results suggested that peroxidase would be connected with regeneration of pepper. Also, esterase activity was increased about 50% more than that of control after 14 days of culture, the pattern of esterase isozyme was shown to be 3 cathodic bands and 1 anodic band after 7 days of culture.ulture.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.377-380
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• 2017

In 2015, a new leaf spot disease was observed on strawberry seedlings in Wanju, Korea. Tanned brown spots appeared on the leaves of the infected plants, and often coalesced to form larger necrotic areas, resulting in the death of foliage. An isolate was obtained in pure culture. On the basis of morphological characteristics and molecular analysis of internal transcribed spacer rDNA sequence, the causal agent was identified as Pilidium concavum. Pathogenicity tests revealed the isolate was pathogenic to the leaves and fruits of strawberry. To the best of our knowledge, this is the first report of P. concavum causing tan-brown leaf spot on strawberry in Korea.

• Journal of the Korean Society of Food Culture
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• v.26 no.6
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• pp.709-716
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• 2011

The principal objective of this study was to evaluate the quality characteristics of dried noodles when different concentrations of Loquat (Eriobotyya japonica Lindley) leaf powder (LLP) were added to the wheat flour. The cooking quality, mechanical texture properties, and viscosity were measured, and a sensory evaluation was conducted with the prepared noodles. The gelatinization points of the composite LLP-wheat flours were shown to increase. As measured via amylograph, viscosity at $95^{\circ}C$, viscosity at $95^{\circ}C$ after 15 minutes, and maximum viscosity values of those samples decreased as the LLP content increased. As increasing amounts of LLP were added, the L and a values were reduced, whereas the b value was increased and the color values, weight, and volume of the cooked noodle increased, as did the turbidity of the soup. With regard to the textural characteristics, the LLP additive increased hardness and cohesiveness, and reduced adhesiveness and springiness. Overall, the noodles prepared with 5% LLP were preferred more than the others, according to the results of our sensory evaluation.

• Journal of the Korean Society of Food Culture
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• v.25 no.2
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• pp.225-231
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• 2010

The principal objective of this study was to evaluate quality characteristics of dried noodles when different concentrations of lotus leaf powder (LLP) were added to the wheat flour, thereby determining which noodle recipe was preferred. The cooking quality, mechanical texture properties, and viscosity were measured, and then a sensory evaluation was conducted with the prepared noodles. The gelatinization points of the composite LLP-wheat flours were shown to have an increased, viscosity at $95^{\circ}C$ after 15 minutes. As measured via amylograph, the maximum viscosity values of those samples were decreased as the LLP content was increased. As well, when increased amounts of LLP were added, both the L and a values were reduced, whereas the b value was increased. The color values, weight and volume of the cooked noodle increased, as did the turbidity of the soup. With regard to the textural characteristics, the LLP additive increased hardness and cohesiveness, and reduced adhesiveness and springiness. Overall, according to the results of our sensory evaluation, the noodles prepared with 5% LLP were preferred over the other noodles.

• Plant Resources
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• v.4 no.3
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• pp.171-180
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• 2001

Hibiscus cannabinus, Cassia fistula and Abutilon indicum are medicinally important plants in India. Tissue culture studies have done for efficient propagation and for crop improvement in these three plants. Various explants were tried for callus induction, somatic embryogenesis and organagenesis with manipulation in culture media. Calli and somatic embryos were induced from hypocotyl explants in Hibiscus cannabinus, while in Cassia fistula and Abutilon indicum it could be obtained from leaf explants.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11a
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• pp.34-48
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• 2001

Hibiscus cannabinus, Cassia fistula and Abutilon indicum are medicinally important plants in India. Tissue culture studies have done for efficient propagation and for crop improvement in these three plants. Various explants were tried for callus induction, somatic embryogenesis and organagenesis with manipulation in culture media. Calli and somatic embryos were induced from hypocotyl explants in Hibiscus cannabinus, while in Cassia fistula and Abutilon indicum it could be obtained from leaf explants.

• Korean Journal of Environmental Agriculture
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• v.19 no.2
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• pp.166-170
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• 2000

This study was conducted in to elucidate the changes of growth characteristics and photosynthesis in three soybean (Glycine max L. cv. Danwonkong, Hwangkeumkong and Kwangankong) 30 day old seedlings to 100mM NaCl concentration containing 1/2 Hoagland's nutrient solution in sand culture. The main stem height and number of main stem node were decreased. thus, leaf area and dry matter were decreased with 100mM NaCl. Growth reduction was less little in Hwangkeumkong than other cultivars. The stem growth rate was affected less than other parts as root or leaf, by NaCl treatment. The specific leaf area (SLA), shoot : root ratio and leaf : root ratio decreased with NaCl solution except for those of Hwangkeumkong. There is no general tendency in leaf thickness by leaf position of three cultivars. The chlorophyll content (SPAD) of the primary and 2nd leaf slightly decreased under NaCl solution but rapidly increased in non-NaCl solution at 15 days after treatments. The photosynthesis, stomatal conductance and transpiration of 2nd leaf positions reduced by NaCl and there were a sigificant correlation between photosynthesis and stomatal conductance or transpiration.