• Preventive Nutrition and Food Science
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• v.19 no.4
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• pp.367-372
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• 2014

Caffeine is widely consumed and well known for stimulating the central nervous system. When developing new foods and beverages that contain caffeine, it is important to explore the potential synergistic effects of consuming amino acids and other food ingredients with caffeine on humans. Given the physiological pathways affected by the amino acid ornithine, consumption of ornithine with caffeine may have synergistic effects. The purpose of the present study was to examine the effect of consuming caffeine with ornithine in humans. The study used a randomized, placebo-controlled, double-blinded crossover design. The subjects were all healthy office workers who ingested the placebo, 100 mg caffeine, or 100 mg caffeine plus 200 mg ornithine in the morning and completed questionnaires about their mood. Office workers who consumed the combination of caffeine and ornithine had higher mood ratings 8 h after consumption than office workers who consumed caffeine alone. The results of the present study suggest that there is a unique synergistic effect between caffeine and ornithine on the mood of healthy office workers and that ornithine may potentiate the effects of caffeine.

• The Korean Journal of Food And Nutrition
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• v.17 no.4
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• pp.442-449
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• 2004

The purpose of this study was to examine the antioxidative activities and tissue cell of mouse liver added caffeine. The body weight of all experimental groups increased during experimental periods, but the body weight of caffeine-containing groups were lower than those of any other experimental groups. Superoxide dismutase and catalase activities tended to decrease significantly with caffeine-containing groups, but increased in control and ginseng-containing groups. Hydroperoxide contents were increase significantly with caffeine-containing groups. Lipid peroxidation levels decreased in ginseng-containing groups, but it increased significantly with caffeine-containing group. Protein contents were a tendency of similar between control and ginseng-containing groups, but it showed a increasing tendency in caffeine-containing groups. Microscopic observation of mouse liver cell were similar tissue in ginseng and caffeine-containing groups, but it showed somewhat more injuring only at the liver cell of anhydrous caffeine group, and became the suspicion in liver diseases. This results show that antioxidative activities are slightly higher in non-caffeine and ginseng-containing drinks than caffeine-cotaining drinks. From this standpoint, we suggest that too much drinking of caffeine-containing drinks for a long time is undesirable.

• Korean Journal of Clinical Pharmacy
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• v.22 no.2
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• pp.167-175
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• 2012

Different studies have been conducted regarding caffeine as psychostimulant drugs and its effects as well as determination in various materials. Since, coffee and tea are most drinkable beverage in the world and their major constituent is caffeine. So, analysis of the effect of equal amount of caffeine in coffee and tea with respect to SD rats was studied. The present methodology was purposed to determine and validate caffeine amount in different brands of coffee and tea, and canvass locomotor and behavioral patterns of SD rats after administrating coffee and tea orally consisting of same amount of caffeine (10, 30 mg/kg). Determination of caffeine in different brands of coffee and tea and validation of caffeine were evaluated using HPLC. Depending upon different brands of tea and coffee, caffeine amount was altered. Meanwhile, amount of caffeine in tea was directly proportional to the temperature of liquid. Coffee and tea (Instant Maxim original coffee$^{(R)}$ and earl grey black tea$^{(R)}$) consisting 10, 30 mg/kg of caffeine were studied in SD rats for locomotor activity and behavioral patterns using Tru Scan 99. The locomotor activities of SD rats were increased after administration of coffee and tea consisting caffeine compared to water. The coffee consisted of higher amount of caffeine exhibited steep movement of SD rats. Similarly, rearing and scratching of SD rats as frequency and duration were increased in coffee and tea consisting caffeine compared with water. The study revealed that coffee and tea had effects in locomotor activity and behavioral patterns of SD rats.

• Korean journal of food and cookery science
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• v.30 no.5
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• pp.650-662
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• 2014

This study was conducted to identify middle school students' caffeine intake from their favorite foods containing caffeine as well as several factors related to their intake. The objects of the investigation were 369 middle school students in the urban area of Changwon-si and 358 middle school students in the rural area of Haman-gun. The average height was 162.3cm, average weight was 52.3 kg and average BMI was 19.8. The food habit score was generally positive at an average of 3.37. The food habit scores of rural area students were higher than those of urban area students (p<.05). The average stress score was somewhat low at 2.82. The stress index for rural students was lower than that of urban students (p<.01). The recognition of caffeine in favorite foods was 8.12 out of a 12 point scale. The average nutritional knowledge score regarding caffeine was very low at 3.08 out of a 10 point scale. The main reason for selecting favorite foods was 'taste'. The percentage of students who don't check food labels was 49.1%. 'House' was the main location while 'good taste' was the biggest reason for eating caffeine-containing foods. The main side effect of caffeine intake was 'nausea'. The average daily caffeine intake for all subjects was 26.96 mg, with the urban students averaging 27.90 mg and the rural students averaging 25.99 mg. The correlations between several factors and caffeine intake were examined. Food habit showed a negative correlation with stress score and caffeine intake. Nutritional knowledge and recognition of caffeine had positive correlations with caffeine intake. Hence nutritional education about caffeine should be emphasized to improve students' healthy food habits.

• Journal of Nutrition and Health
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• v.43 no.5
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• pp.475-488
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• 2010

The daily caffeine intake from elementary school children's favorite foods was surveyed and evaluated. Children may respond to caffeine differently from adults because they have different physiological makeup and are functionally immature. Therefore, caffeine exposure may have more serious consequences for children than for adults, irrespective of sensitivity. Their preference, perception, and intake of caffeine from children's favorite foods were investigated by questionnaire for 355 children. The order of children's preference over foods containing caffeine was ice cream and ices, confectionary, milk and milk products, and soft beverage. The daily caffeine intake of children was estimated to range from 0.16 to 917.28 mg/day, with an average of $36.04\;{\pm}\;82.7$ mg/day and $36.9\;{\pm}\;96.0$ mg/day for boys and girls, respectively. The daily caffeine intake according to body weight was $1.08\;{\pm}\;2.23$ mg/kg and $1.12\;{\pm}\;2.66$ mg/kg for boys and girls, respectively. The percentage of acceptable daily intake (ADI) of caffeine was 43.4% for boys and 44.9% for girls. The sources of caffeine for boys and girls were soft beverage (18.3 mg and 16.1 mg), milk and milk products (8.9 mg and 8.5 mg), ice cream and ices (5.7 mg and 7.3 mg), chocolate (1.6 mg and 3.2 mg), and confectionery (1.6 mg and 1.8 mg).

• The Korean Journal of Zoology
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• v.19 no.4
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• pp.161-170
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• 1976

Since caffeine inhibits the active uptake of Ca by the sarcoplasmic reticulum, the action of caffeine on the fragmented sarcoplasmic reticulum of rabbit skeletal muscle was studied. Caffeine seemed not to bind tightly to the sarcoplasmic reticulum. The determination of sulfhydryl content of the fragmented sarcoplasmic reticulum, however, suggested that caffeine in some unknown manner influences the protein moiety and thereby increases the sulfhydryl content. The inhibition of Ca uptake by caffeine therefore might be considered as due to the result of this change in protein sulfhydryl content.

• Journal of the Korean Home Economics Association
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• v.12 no.34
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• pp.737-741
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• 1974

Coffee is a popular beverage with many people at home and in the tea-room, Caffeine is a major chemical compound of the coffee. A little caffeine is a stimulant to the movement and spiritual nerve, but much caffeine influences sleep, nervousness, non-concentration, headache and giddliness depending upon each person. The writhe expresses the result of the caffeine content in the coffee which is collected from the coffee-shapes of Seoul. The contents are as follows : 1) Caffeine content of the coffee of the tea-room was researched dividing into the contral Seoul city and the outskirts of Seoul. 2) Caffeine content of the coffee in the outskirts tea-rooms is 0.32-1.23 mg/ml and the average is 0.546 mg/ml (SD 0.19). 3) Caffeine content of the coffee of the central city is 0.34-0.73 mg/ml and the average is 0.513 mg/ml (SD 0.12). 4) There was no difference between the outskirts and the central city. 5) Caffeine content of the coffee of the tea-rooms according to each season is going to be researched.

• Journal of Nutrition and Health
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• v.30 no.10
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• pp.1160-1169
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• 1997

The purpose of this research was to investigate the effects of caffeine and calcium levels on calcium utilization in female rats of different ages. Calcium utilization was compared in female rats of different age( 4 weeks and 12 months) fed various levels of caffeine(0 and 7 mg/100g body weight) and calcium (50, 100 and 200% of requirement) for 3 weeks. Feed intake of the caffeine groups were lower than that of the no-caffeine groups. body weight gain was lowest in the high-caffeine and low-calcium group. Serum calcium levels of young rats were higher than those of adult rats. There were no significant differences in tibial calcium content among the caffeine and calcium -groups. Fecal calcium excretion increased as the level of dietary calcium was increased. Urinary calcium excretion increased as the levels of caffeine and dietary calcium were increased. With increasing levels of dietary calcium , daily calcium retention was accelerated, but apparent calcium absorbability was diminished. The results of this study suggest that caffeine consumption promotes urinary calcium excretion. However, increase in dietary calcium resulted in higher calcium retention . These findings indicate that high caffeine consumption may increase dietary calcium requirements. Therefore, it could be suggested that the supplementation of dietary calcium may counteract the negative effect of caffeine intake on calcium utilization.

• Journal of Nutrition and Health
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• v.29 no.9
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• pp.950-957
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• 1996

This study was designed to investigate the effects of caffeine intake on Ca and P metabolism in ovariectomized rats. For this purpose, the ovariectomized female rats weighing 230.8$\pm$3.5g were divided into four groups, eight rats each ; control, low caffeine(LC), medium caffeine(MC)and high caffeine(HC) groups. They were supplied for six weeks with the caffeine of 0mg, 66.8mg, 167mg and 334mg per kilogram of diet, respectively, and the results are summarized as follows. 1) There were no significant differences in feed intake, feed efficiency ratio and body weight change among all of experimental groups. But liver weight(both total weight and weight/body weight) was significantly decreased by caffeine in MC group. 2) The weight of tibia was decreased by caffeine intake in MC and HC groups. But the length, Ca and P content of tibia and femur was not changed in all of caffeine groups. 3) Ca, PTH and calcitonin levels in serum were not affected by caffeine. While, serum P level in HC group was significantly increased as compared with the control. 4) The fecal excretion of Ca and P tended to be higher in the caffeine groups, and as the result, the absorption rate, retention and retention rate of Ca and P tended to be decreased. It was noteworthy that P retention was significantly lowered in HC group as compared with LC group. The results showed that, when caffeine was taken by ovarietomized rats, the weight of tibia was decreased and the retention rate of Ca and P tended to be lowered. Therefore, too much intake of caffeine for women whose bone mineral density of tibia is decreased after postmenopause seems to accelerate the decrease of bone mineral density due to the negative effect on metabolism of Ca and P.

• Toxicological Research
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• v.33 no.2
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• pp.157-164
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• 2017

This study was to evaluate the age-dependent effects of caffeine exposure on the long bones and reproductive organs using male rats. A total of 15 immature male rats and 15 young adult male rats were allocated randomly to three groups: a control group and two groups fed caffeine with 120 and 180 mg/kg/day for 4 weeks. Exposure to caffeine at either dose significantly reduced body weight gain; a proportional reduction in muscle and fat mass in immature animals, whereas a selective reduction in fat mass with relatively preserved muscle mass in young adult animals. The long bones of immature rats exposed to caffeine were significantly shorter and lighter than those of control animals along with decreased bone minerals. However, there was no difference in the length or weight of the long bones in young adult rats exposed to caffeine. Exposure to caffeine reduced the size and absolute weight of the testes significantly in immature animals in comparison to control animals, but not in young adult animals exposed to caffeine. In contrast, the adrenal glands were significantly heavier in caffeine-fed young adult rats in comparison to control animals, but not in caffeine-fed immature rats. Our results clearly show that the negative effects of caffeine on the long bones and testes in rats are different according to the age of the rat at the time of exposure, and might therefore be caused by changes to organ sensitivity and metabolic rate at different developmental stages. Although the long bones and testes are more susceptible to caffeine during puberty, caffeine has negative effects on body fat, bone minerals and the adrenal glands when exposure occurs during young adulthood. There is a need, therefore, to educate the public the potential dangers of caffeine consumption during puberty and young adulthood.