• Journal of Plant Biology
• /
• v.17 no.3
• /
• pp.107-112
• /
• 1974

Leaf temperature is complicated with the microclimate and the dry matter production in a plant community. But a daily change of leaf temperature varying by the locality in plant body or plant community is not yet clear. To resolve such a question, following experiment was designed; Helianthus tuberosus L., Glycine max L., Zea mays L., Impomoea batatas Lam., and Cucurbita moschata var. toonas Makino were planted in the different sandy loam, $2m{\times}2m-quadrat$, which has a eastern, southern, western and northern edge. In each plot 17-25 plants were planted and the distances between individuals spaced uniformly. And leaf temperature were measured by MR3-C type thermistor from 14th May through 20th August. It is seems that the upper leaf is affected by solar radiation, the lower leaf by released heat from the earth and the middle leaf by the conditions of both zones. Measuring the temperature of a leaf that is on terminal, central, left and right margins and base part, temperature of control plant in a leaf was sloped during about two hours from noon. It is noticeable as a "noon sleeping" phenomenon.henomenon.

• Journal of Plant Biology
• /
• v.32 no.2
• /
• pp.109-119
• /
• 1989

Adhatoda vasica Nees is a shrubby component of tropical and subtropical vegetation in Pakistan. t harbours relatively few unhealthy associated species in and around its thickets. Aqueous extracts, rain leachates, litter from shoots and soil underneath it invariably reduced germination, early growth, biomass, moisture and chlorophyll contents of Pennisetum americanum, Setaria italica, Zea mays, Brassica campestris and Triticum vulgare in different laboratory expreiments. chromatographic analysis revealed the presence of caffeic, ferulic, vanillic, p-coumaric, p-OH-benzoic, and tannic acids in aqueous extracts. The phytotoxicity was related to the test species used, part assayed and parameter measured. It is suggested that the preclusion of the associated species and the dominance of A. vasica is primarily due to allelopathy. Negative grazing also provides better chances for its establishment.

• Journal of the Korean Society of Tobacco Science
• /
• v.13 no.2
• /
• pp.34-41
• /
• 1991

To explore the possibility of introducing Zea mays transposable element Ac(activator) which can be used as a mutagen and gene tag in tobacco plants other than maitre, we tried to introduce a cloned Ac element into tobacco cells by an Agrobacterium tumefaciens binary vector system. Transformation of N. babacum cv. Burley 21 tissues and regeneration to whole plant were carried out. The frequency of the transformed callus induced in shoot induction media was higher than that of transformed callus induced in callus induction media. However, the calli were not grown in the second selection media, and became yellow senescent calli. Regenerated tobacco plantlets with foreign gene were also obtained in shoot induction media containing 100 $\mu\textrm{g}$/ml kanamycin and 100$\mu\textrm{g}$/ml carbenicillin. The leaf tissues of transformant was also resistant to 1000 $\mu\textrm{g}$/ml kanamycin. The chromosomal DNAs of transformant and normal plant of N. tabacum were digested by EcoR I and Hind III but not by Pst I.

• BMB Reports
• /
• v.29 no.4
• /
• pp.327-334
• /
• 1996

In this study, the origin of the monovinyl chlorophyll a carboxylic biosynthetic route was investigated in barley (Hordeum vulgare L.) and com (Zea mays L.). Protoporphyrin IX accumulated in vivo or in vitro was found to be all of the divinyl form. Furthermore, the conversion of divinyl protoporphyrin IX to monovinyl protoporphyrin IX in vitro was not observed. In contrast, the biosynthesis and accumulation of monovinyl Mg-protoporphyrin IX and its methyl ester occurred in etiolated leaves and divinyl Mg-protoporphyrin IX was convertible to monovinyl Mg-protoporphyrin IX in vitro. These results suggest that the monovinyl chlorophyll ${\alpha}$ carboxylic biosynthetic route in plants may originate from the divinyl Mg-protoporphyrin IX pool.

• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.106-112
• /
• 2008

International conferences to block the spread of Avian bird flu occurred in Beijing, 2006 and others warned of the seriousness of the H5N1 strain. The meetings succeeded in generating billions of dollars from USA, EU and World Bank. Migratory birds seem to play a major role in the spread of the aggressive strain globally from Asia to Europe and Africa. Experiences of tolerance breeding of maize (Zea mays L.) for four decades against 20 biotic stresses suggest that the prime cause of the occurrence of H5N1 strain was due to the human beings' counter-efforts against nature. Excessive use of chemicals (spray and injection) in the commercial poultry farms had created high selection pressure on virus. The new strain had mutated for survival. Attempting to eliminate the virus by chemicals for 100% control is a dangerous way to control biotic stresses. This can create more aggressive strains. A solution would be to build up tolerability of the commercial animals against the virus. Improvement of poultry cage environments and respect for nature must be integrated. Potential foes must be watched.

• Journal of Plant Biology
• /
• v.33 no.4
• /
• pp.315-320
• /
• 1990

We investigated the interaction of auxin and Ca2+ on coleoptile segment elongation in seedlings of Zea mays L. Seedlings imbibed and raised either in the presence of 10 mM CaCl2 (HC), or in the absence of Ca2+ (LC) were used. Exposure to 10-5M auxin of coleoptiles from either HC or LC seedlings resulted in strong promotion of elongation. However, longer latent period (90 min) of the auxin effect was observed in HC than in LC seedlings (20 min). The length of latent period observed in HC coleoptiles was proportional to the concentration of CaCl2. The latent period of auxin effect observed in HC seedlings was abolished by pretreatment of the coleoptiles with TMB-8 which inhibits IP3-induced Ca2+ release from the tonoplasts. In segments of LC seedlings, the promotive effect of IAA (10-5M) was abolished by treatment with 5 mM calcium but was reversible upon treatment of the segments with 5 mM EGTA. These results suggest that the effect of auxin on coleoptile elongation is closely related to intracellular Ca2+ level.

• Mycobiology
• /
• v.37 no.1
• /
• pp.72-76
• /
• 2009

To investigate the growth response of various crop species to mycorrhizal inoculation, arbuscular mycorrhizal fungi were applied to Glycine max, Vigna angularis, Senna tora, Hordeum vulgare var. hexastichon. Zea mays, Sorghum bicolor, Allium tuberosum, Solanum melongena, and Capsicum annuum. The biomass of the inoculated crops was measured every two weeks for the 12-week growth period. By measuring biomass, we calculated the mycorrhizal responsiveness of the nine crop species. Among the nine crop species, four species showed a significant response to mycorrhizal inoculation. The shoot biomasses of V. angularis, C. annuum, A. tuberosum, and S. tora significantly increased with mycorrhizal inoculation.

• Journal of Plant Biology
• /
• v.36 no.3
• /
• pp.203-210
• /
• 1993

In order to investigate the effects of plant hormones on the quantitative changes in mRNA of maize (Zea mays L.) rbcL, we used GA3, IAA, ABA and BAP. GA3 at the concentration of 10-4M resulted in decrease in rbcL gene transcript to 62%. IAA decreased the amount of rbcL transcript to about 70% at all the hormone concentrations tested. ABA did not cause a noticeable change in the amount of rbcL transcript, but BAP increased the amount of rbcL transcript to 153% at 10-8M and 123% at 10-5M, respectively. Thus, it appears that BAP is related to the increase in the amount of rbcL transcript by light.

• Journal of Plant Biology
• /
• v.36 no.1
• /
• pp.67-74
• /
• 1993

Both polar and gravity-induced lateral transport of auxin was markedly reduced in corn coleoptile segments by octanol treatment. Octanol enhance net auxin uptake without affecting that of benzoic acid, suggesting that the effect did not result from a nonspecific action on general membrane permeability. Since naphthylphthalamic acid (NPA) action on both transport and net uptake of auxin was substantially decreased in the presence of octanol, a specific interaction of octanol with the NPA site (efflux carrier) can be postulated. Studies on in vitro binding of NPA to membrane vesicles indicated that octanol did not interfere with NPA binding. When basipetal transport of auxin was impared by plasmolysis, octanol still inhibited auxin transport in the plasmolyzed tissues. The results ruled out the possibility of octanol acting at the plasmodesmata. Kinetic analysis of growth indicated that IAA-sustained growth was rapidly blocked by octanol implicating a common system by which auxin transport is linked to auxin action. Possible mechanisms for octanol action will be discussed.

• Plant Biotechnology Reports
• /
• v.2 no.4
• /
• pp.267-270
• /
• 2008

We have successfully used the low-pressure BioWare gene gun, developed for gene transfer in animal cells, for plant tissues. The BioWare device is easy to manipulate. Just 50 psi helium pressure was sufficient to transfer foreign genes into the aleurone layer and embryo of maize without causing tissue damage in the impact area. As shown by expression signals from invasive histochemical ${\beta}-glucuronidase$ (GUS) activity, the foreign reporter gene expressed well in bombarded tissues. This successful GUS-transient expression extends the application of this low-pressure gene gun from animal cells to plant tissues.