• Journal of Microbiology
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• 제43권2호
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• pp.172-178
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• 2005

To determine the prevalence and genotypes of $\beta$-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced $\beta$-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five ($167\%$) of thirty clones produced an extended-spectrum $\beta$-lactamase. 837- and 259-bp fragments specific to bla$_{TEM}$ genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM­1 was the most prevalent $\beta$-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type $\beta$-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type $\beta$-lactamases of the pre-antibiotic era indicates that TEM-type $\beta$-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

• 한국한의학연구원논문집
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• 제2권1호
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• pp.269-288
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• 1996

Coicis Semen is one of the oriental medicine that has been used for the treatment of the diseases such as pulmonary abscess, periappendicular abscess and wart since ancient times. However, the mechanism of the action of the drug is not well studied. This study was done to investigate the effects of Coicis Semen on the host defence mechanism. Effects of Coicis Semen on the immune responses were analysed by measuring the contact hypersensitivity, hemagglutinin, hemolysin and rosette formation, cytotoxicity, and reactive oxygen intermidiates production. As the results, water extract of Coicis Semen administration enhanced the antibodies (hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen. Also Coicis Semen increased the allogeneic immune response in the mouse, showed cytotoxic activity against human leukemia cell line(K562) and decreased the contact hypersensitivity against dinitroflurobenzene. Also administration of Coicis Senlen slightly increased NK cell activity and enhanced the production of such reactive oxygen intermediates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. The above results demonstrate that Coicis Semen has enhancing effects on cellular and humoral immune responses against disease.

• 동의생리병리학회지
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• 제18권1호
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• pp.28-38
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• 2004

The Hun-Min-Jeong-Eum(訓民正音) has been created under the political purpose that is to unite politics and cultures through a philosophical harmony of the confucianism as well as the Buddhism under the periodical circumstance that includes the change of a dynasty and a revolution in culture. The creating work has been greatly affected by the theoretical divination, that is the confucian ideas of North-Song dynasty which has been highly elaborated in those days. The shape of the characters of the Hun-Min-Jeong-Eum(訓民正音) has been influenced by the ancient letters existing at the time (especially Ga-Rim-To 加臨多), it has been throughly readjusted and recreated based on the philosophy. The consonant uses the Three-Pillars(三才)(ㅇ, ㅁ, △) in the style of the five elements and each velar-lingual-labial-dental-guttural sounds (牙舌脣齒喉音) is applicable to five elements also a 'ㅇ' is equivalent to the heaven, a 'ㅁ' to the earth. a '△' to the man. The vowel uses the five elements of ㅗ(water). ㅏ(wood), ㅜ(fire), ㅓ(metal), ㆍ(five-soil), ─(ten-soil) in the style of the Three-Pillars(三才) of ㆍ, ㅡ, ㅣ and the first created letters(初出字), and the secondary created letters(再出字) each can be attached to the eight trigrams(八卦). The consonant has 17 letters and the vowel has 11 letters, therefore the total number of the letters is 28. and those are equivalent to the 28 constellation(二十八宿) of the astronomy. Contrary to the underlying principle that has been applied during the time of the creation of the letters, when the consonant and the vowel are used, the consonant refers to the heaven (天), and the vowel refers to the earth(地). a consonant take a part in making a point on the starting place of the pronounce, a vowel take a part in the keeping the energy to the end.

• 한국문헌정보학회지
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• 제45권3호
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• pp.107-140
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• 2011

이 연구는 1855(철종 6)년에 배상현(裴象鉉)이 저술한 문물 제도서인 "동국십지"의 10지(志) 중 하나인 "예문지"를 분석하였다. "동국십지" "예문지"에는 고구려, 신라, 고려 및 조선의 서적과 중국의 서적 137종을 수록하고 있으며, "예문지" 나름의 15류(類)의 분류 및 배열체계를 지닌다. 또한 "예문지"에서 서적의 수록은 편찬된 시대, 찬자(撰者), 서명(書名) 등 일부는 서적의 해제까지 붙어 있다. 그러나 찬자 배상현의 분류체계는 "동국십지"가 모방한 "한서" "예문지"나 비슷한 시기에 편찬된 "동국통지" "예문지"의 분류체계와 비교한 결과, 경서(經書) 등 일부분을 자세히 확립된 반면 일부 주제와 관련하여 체계가 설정되지 않았다. "동국십지" "예문지"의 수록도서는 한국 찬술도서와 중국 찬술도서로 구별되며, 대부분 한국 찬술도서로 구성되었다. 수록도서의 주제는 사부분류로 분석한 결과, 경(經) > 자(子) > 집(集) > 사(史) 순으로 많았으나 그 종수가 크게 차이나지 않는다. 연구대상인 "동국십지" "예문지"는 통시대적이고 종합적 문물제도서로서, 비록 완벽한 체제와 내용을 갖추지 못하였으나 개인의 힘으로 그 당시의 서적들을 수록했다는 점에서 가치가 있다.

• 대한한의학회지
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• 제19권1호
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• pp.220-233
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• 1998

This study was made to objectify the principle of oriental medical prescriptions so that we could obtain the principle and the formulation of them For that purpose, we analysed the formula and rule of 205 prescriptions recorded in Gum-goe-yo-rak, which have a few of components, and are widely used in clinical from ancient times to these days. At first we classified those prescriptions by their effect. Then we re-classified the herbs of the effect groups into four natures (cold, hot, warm and cool) and five kinds of flavors (sour, bitter, sweet, acrid and salty). And we classified the herbs into three grades - superior, medium, and inferior - which were used in Shen Nong's Herbal Classic. By these means, we statistically evaluated the prescriptions recorded in Gum-goe-yo-rak based on the Four Regular Components(Monarch, Minister, Assistant, and Laborer). As a result we could obtain some facts about the prescriptions recorded in Gum-goe-yo-rak, those are what kind of herbal material was used frequently and distribution of the natures flavors and grades according to thier effects. And we are sure that these results can be great help for establishment of fundamental formulation of theory of traditional herbal medicinal polyprescription.

• 패션비즈니스
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• 제16권2호
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• pp.44-61
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• 2012

The 21st century directs designomics to achieve wealth by designing old beyond a simple recognition of the importance of culture. One of the representative local cultures of Gyeongnam is Gaya culture. The Gaya period had over 600 years history with rich and excellent iron manufacturing skill and advanced pottery culture. It is the very time to reevaluate the importance of Gaya equivalent to the Three Kingdoms in our ancient history which was has been so far ignored and isolated. In order to develop unique and excellent culture of Gaya as local fashion cutural goods, questionnaire survey on the recognition of Gaya culture and cultural goods purchasing status had been conducted to target visitors of Representative National Museum. Data Analysis Using SPSS 18.0 Win was performed with frequency analysis and t-test. As to the questionnaire survey, important criteria for purchasing in case of buying cultural goods could be found and the residents in Gyeongnam had higher recognition of developing cultural goods by the motif of Gaya relics. 'Mounted vessel in the shape of warrior on horseback(12.4%)' and 'Chariot wheel-shaped Pottery(10.8%)' were selected the most suitable motif, 'Cellphone strap(11.0%)' and 'key holder(10.2%), 'cup (7.2%)'were selected the most suitable item of cutural goods. These will be actively reflected in the development of fashion cultural goods design applying Gaya relics.

• 한국의상디자인학회지
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• 제16권2호
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• pp.101-109
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• 2014

Bangryeong generally means rectangular collars at present. However, it is estimated that there would be Bangryeong in different shapes besides rectangular shape in ancient times, but there is no accurate evidence. The dispute on the shape of Bangryeong started with the records in Yegi(禮記) and Simui written during Zhou period, China, the oldest records on Bangryeong. However, there are no remains or paintings(pictorial data) accurately proving the different shapes of Bangryeong. While the new shape of Gokgeop(collar of Simui) was mentioned by Zhu Xi during Song period, it was not accurately verified. There are almost no literature, excavated objects or pictorial data on the shapes of Bangryeong which are not definitely identified. The shape of Bangryeong is identified to a certain extent by objects excavated or pictorial data during Yuan and Ming period. There will be definite reasons why Zhou Xibao and Hua Mei, the scholars in China, the y-shaped collar rolled on the chest among the objects excavated during Warring States Period as Bangryeong or Guryeong. Thus, this paper investigated the notes and description in Yegijeongui(禮記正義) and the records and notes during the Han period which are mentioned most frequently except Yegi among the records on Bangryeong. Accordingly, this paper identified that there are different shapes of Bangryeong except rectangular shape during the Han period. It is estimated that Bangyeong at that time had symmetric collars, the collars of Jikryeong which are not overlapped. The collar section after the neck section was straight. It was also said that Bangyeong was women's clothing. In other words, Bangyeong in the Han period suggests that the collar of Jikryeong was Bangryeong. As discussed above, Bangryeong has the long history and different meanings by period. However, it is very difficult to accurately define Bangryeong because there are almost no records on it. Nevertheless, Bangryeong will be discussed more in further study.

• Journal of Astronomy and Space Sciences
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• 제32권1호
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• pp.63-71
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• 2015

During the reign of King Sejong (世宗, 1418-1450) in the Joseon Dynasty, there were lots of astronomical instruments, including miniaturized ones. Those instruments utilized the technical know-how acquired through building contemporary astronomical instruments previously developed in the Song(宋), Jin(金), and Yuan(元) dynasties of China. In those days, many astronomical instruments had circles, rings, and spheres carved with a scale of 365.25, 100, and 24 parts, respectively, on their circumference. These were called the celestial-circumference degree, hundred-interval (Baekgak), and 24 direction, respectively. These scales are marked by the angular distance, not by the angle. Therefore, these circles, rings, and spheres had to be optimized in size to accomodate proper scales. Assuming that the scale system is composed of integer multiples of unit length, we studied the sizes of circles by referring to old articles and investigating existing artifacts. We discovered that the star chart of Cheonsang yeolcha bunyajido was drawn with a royal standard ruler (周尺) based on the unit length of 207 mm. Interestingly, its circumference was marked by the unit scale of 3 puns per 1 du (or degree) like Honsang (a celestial globe). We also found that Hyeonju ilgu (a equatorial sundial) has a Baekgak disk on a scale of 1 pun per 1 gak (that is an interval of time similar to a quarter). This study contributes to the analysis of specifications of numerous circular elements from old Korean astronomical instruments.

• 전자공학회논문지SC
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• 제47권5호
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• pp.52-61
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• 2010

최근 피부의 마찰계수를 이용하여 한의학에서의 체질을 구별하는 연구가 활발히 이루어지고 있다. 피부의 마찰계수는 여러 고문헌에서도 알려져 있듯이 태음인과 소양인의 체질을 구별하는 인자로 활용되고 있다. 본 연구에서는 다축 로드셀과 일정하중식 접촉 프로브를 이용하여 피부의 마찰계수를 정량적으로 측정하는 장비를 개발하였고 이의 측정 불확도를 측정하였다. 개발된 장비의 상대 반복도는 2 %였다. 태음인과 소양인의 체질진단 구분을 위해 왼속 손등을 측정부위로 선택하여 실험한 결과 약지를 따라 측정하는 것이 가장 효과적임을 알 수 있었다. 20명의 피험자를 대상으로 임상측정을 한 결과, 개발된 장비의 경우 3 % 이내의 측정 불확도를 가지고 태음인과 소양인의 체질 구분을 할 수 있었다.

• 대한본초학회지
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• 제38권5호
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• pp.21-29
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• 2023

Objects : This study aimed to identify the origin species of Ulmi Cortex and Ulmi Semen Pasta. Methods : I searched for different types of 'Yu(楡)' from Zhong Xiu Zheng Huo Jing Shi Zheng Lei Bei Yong Ben Cao (重修政和經史證類備用本草) of the Song Dynasty, then compared the descriptions of Yu with real life species. Items that were inconsistent were sequentially deleted to finally find the original species that satisfies all conditions. Results : There were five types of Yu : Jiyu(地楡), Jayu(刺楡), Langyu(郞楡), Goyu(姑楡), and Baekyu(白楡). Jiyu was Sanguisorba officinalis or Sanguisorba officinalis var. longifolia which was a herb. Jayu was Hemiptelea davidii (Hance) Planch. which had thorns on its branches. Ulmus laciniata had leaves that split into three branches. Langyu was Ulmus parvifolia which beared fruit in the fall. For these reasons, the four items above were ruled out. The fruit of Goyu was Ulmi Semen Pasta, which was the largest among fruits and was therefore Ulmus macrocarpa. The fruit of Baekyu was Yuhyup(楡莢) and the ancient coin Yuhyupjeon(楡莢錢) was resembled Yuhyup. The normal pulse of the lung resembled the appearance of falling Yuhyup. The peel of Baekyu was Yubaekpi(楡白皮), and when it was removed from coarse bark, contained an abundance of white sap; the place where this sap overflowed on the stem bark was white, therefore Baekyu was identified as Ulmus pumila. Conclusions : From the results above, the origin species of Ulmi cortex was Ulmus pumila and the origin species of Ulmi Semen Pasta was Ulmus macrocarpa.