• Title/Summary/Keyword: analysis of sedimentation

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The abundant presence of nonpolyadenylated SV40 late 19S spliced RNA in the nucleus of monkey cell (Poly A tail을 결핍한 Simian virus 40 spliced RNA의 세포내 분포)

  • ;Mertz, Janet
    • Korean Journal of Microbiology
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    • v.26 no.2
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    • pp.106-112
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    • 1988
  • We have examined the structures and cellular distributions of the SV40 late RNAs present in monkey cells at late times after infection. One particular RNA species, spliced at residue 373(373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analysis indicated that the 373-RNA was approximately 16S to 19S in size. Therefore, it was not a splicing intermediate or the product of premature termination of transcription within the late leader region. Whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack poly A, and be located in the nucleus.

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Analysis of Flow Around Multi-Circular Cylinders Using a Numerical Model (수치모형을 이용한 다원주 주위의 유동 해석)

  • Lee Sang-Hwa;Park Jung-Ho
    • Journal of Ocean Engineering and Technology
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    • v.20 no.2 s.69
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    • pp.29-35
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    • 2006
  • The flow patterns around multi-circular cylinders are studies, in order to obtain a global view on the structure of wave control and circulation of sea flow in coastal region. The flow force depends upon the vortex shedding exerted on the structure, especially how the vortex shedding affects the erosion when the structure sets on the sand bottom. Therefore, it is necessary that the flow pattern be hocked. In order to simulate the flow around multi-circular cylinders, the CFX and FLUENT of the computational fluid dynamics (CFD) program were used and compared with the experimental results of the flow visualization installation. The phenomena of flow around the multi-cylinders will be applied to fundamental data for predicting the flow force acting against the structure, erosion and sedimentation around cylinders in arrangement.

Numerical Analysis of A Vortex Cyclone in A Recirculating Aquaculture System (순환여과 양식장에서 와류형 분리기에 대한 수치적 검토)

  • Jeong, S.K.;Kim, E.
    • Journal of Power System Engineering
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    • v.9 no.1
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    • pp.36-41
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    • 2005
  • This study investigated the performance of a vortex hydrocyclone for solid removal in a recirculating aquaculture system. In a fish-breeding industry, effluent water is mainly disposed by gravity sedimentation. Thus, a large settling tank and a lot of water are needed to purify effluent water. However, this typical method does not show consistent efficiency. In case of low efficiency, discharged water contains a lot of feeding sediments. This causes environmental problems. Instead of this typical method a hydrocylone is tested to discharge water which contains a lot of feeding sediments. In this paper, a hydrocyclone with low velocity and pressure drop in a recirculating aquaculture system is investigated.

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Purification and Characterization of a 15S Arpase from Chick Skeletal Muscle (계 골격근에서 15S ATPase의 순수분리 및 특성연구)

  • 심규석;채광수
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.524-530
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    • 1994
  • An Arpase complex has been purified to apparent homogeniety from the extract of chick skeletal muscle using conventional column chromatographies and glycerol density gradient centrifugation. This eWe has a sedimentation coefficient of 15S as determined by the gradient centrifugation and therefore is referred to as the 15S ATPase. It behaves as a 600-kOa molecule upon gel filtration analysis using a Superose-6 column. However, the ATPase runs as a 95-kDa polvpeptide when analyzed by polvacrvlamide gel electrophoresis in the presence of sodium dodecyl sulfate. Thus, the Arpase is likely to consist of six identical subunits of 95 KDa. It has a Km value of 0.6 mM for ATP and is maximally active at pH 9.

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Flow Analysis of the Rotating Sludge Suction Collector by Numerical Simulation (수치시뮬레이션을 이용한 흡입식 슬러지 수집기의 유동해석)

  • Suh, Sang-Ho;Roh, Hyung-Woon;Byun, Jong-Youn
    • The KSFM Journal of Fluid Machinery
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    • v.9 no.5 s.38
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    • pp.22-27
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    • 2006
  • Sedimentation phenomenon of suspended solids occurs by the gravitational force. Pollution particles are separated from slowly flowing waste water in clarifier. Recently, the sludge suction collector is Preferred rather than the scraper type sludge collector due to the enhancement of the clarifier efficiency. The sludge suction collector is usually operated by the user's experiences without any scientific and/or technical consideration. To evaluate the performance of sludge suction collector, the three dimensional numerical simulation was conducted by the finite volume method. To analyze the performance, the velocity vectors and the suction flow rates of the orifices were investigated. The result showed that each suction flow rate through out the collector was equivalent in the sludge suction collector and the efficiency of suction collector was good to remove high concentrated sludge in clarifier.

Characterization of Non-linear Consolidation of Dredged Soil from Incheon Area (인천 지역 준설토의 비선형 압밀특성 연구)

  • Oak, Young-Suk;An, Yong-Hoon;Lee, Chul-Ho;Choi, Hang-Seok
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.10a
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    • pp.1693-1706
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    • 2008
  • It is of importance to determine the zero effective stress void ratio($e_{00}$), which is the void ratio at effective stress equal to zero, and the relationships of void ratio-effective stress and of void ratio-hydraulic conductivity for characterizing non-liner finite strain consolidation behavior for ultra-soft dredged materials. The zero effective stress void ratio means a transitional status from sedimentation to self-weight consolidation of very soft soil deposits, and acts as a starting point for self-weight consolidation in the non-linear finite strain numerical analysis such as PSDDF. In this paper, a new method for determining the zero effective stress void ratio has been introduced with the aid of measuring electrical resistivity of the specimen. A correlation between the zero effective stress void ratio and the initial slurry void ratio has been proposed, which can be used in PSDDF analysis as an input parameter. Combining all of the accessible experimental data, the consolidation characteristics of a dredged soil from the Incheon area has been studied in detail.

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Development of Standard Guideline for Water-leakage and Maintenance by parts of the Underground Structures in Multi-Family Housing - A Case Analysis of Water Leak Diagnosis - (공동주택 지하구조물의 부위별 표준 누수 진단 유지관리 지침 개발 - 누수 진단 사례 분석 -)

  • Kim, Soo Yeon;Lee, Jung Hun;Song, Je Young;Jang, Duk Bae;Oh, Sang Keun
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.05a
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    • pp.142-143
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    • 2017
  • An analysis of the current water leak status of understand structure (underground parking lots, staircases, plumbing systems, water reservoirs, etc.) of multi-family housing in South Korea shows that water leaks are found from cracks in all areas of the underground structure caused by the degradation environment (water pressure by underground water, humidity, temperature, earth pressure, soil behaviour and vibration, etc.), which result in various problems, including mold, malodour, debonding of finishing materials, exfoliation, breakout, water leaks in electrical boxes, efflorescence, sedimentation of calcium hydroxide, decoloration, rusting, damages and pollution among others. Therefore, this study aims to analyse the current status of water leaks in underground structure and use the results as the basic data for developing a standard guideline for water leaks and maintenance by parts of the underground structure of multi-family housing.

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The Reaction Efficiency and Surface Characteristics for Metallic Ions in Air Flotation Process (부상공정에서 금속이온의 기포 표면 전위 특성 및 반응효율)

  • Han, Moo-Young;Dockko, Seok;Kim, Young-Hwan
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.2
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    • pp.222-227
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    • 2004
  • Flotation processes involve the use of very small bubbles (micro-bubbles) to separate particles from water. The process has become a good alternative to sedimentation, especially where the particles are small or of low density. Although the flotation process commences with a collision between particles and bubbles, most research has been focused only on the characteristics of the particles. In this paper, recent theoretical and experimental research on the characteristics of bubbles is summarized. The effect on the collision efficiency of the size and charge of bubbles is calculated through trajectory analysis. The size and charge of bubbles are measured under different conditions and the ramifications of the results are discussed. The results may lead to a better understanding and optimization of the existing process. In particular, we discuss an idea that a new advanced flotation process might be possible by the modification of the characteristics of the bubble alone or of both bubble and particle.

The Estimation of Compacted State on Sea Dike Embankment with the Interrelationships Between the Hydraulic Head Loss Rate, the Hydraulic Conductivity and the Void Ratio (수두손실률, 투수계수 및 공극비의 상호관계를 통한 제체의 다짐상태 평가)

  • Eam, Sung Hoon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.57 no.1
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    • pp.11-23
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    • 2015
  • In this study the laboratory test for hydraulic conductivity and the seepage analysis with finite element method on measurement section of sea dike embankment were performed for the purpose of estimating the relative density of embankment from the measured pore water pressures, and both results of the test and the analysis were coupled with the method of estimating seepage blocking state with the hydraulic head loss rate in sea dike embankment. The relationship of void ratio vs hydraulic head loss rate was obtained by setting hydraulic conductivity as common ordinate on the relationships between the void ratio and the hydraulic conductivity and between the hydraulic conductivity and the hydraulic head loss rate. The void ratio on the segment between measuring points was calculated from the coupled relationship of the void ratio vs the hydraulic conductivity. The allowable upper and lower limits of hydraulic head loss rate and those of void ratio on the safety were generated from the coupled relationship between the laboratory compaction test and the sedimentation test. Current hydraulic head loss rate and void ratio were evaluated in the allowable range between upper and lower limits.

Optimization of Distribution Basin Weirs at a Sewage Treatment Plant Based on Computational Fluid Analysis Using the Taguchi and Minitab Method (전산유체해석과 다구찌 및 미니탭 방법을 활용한 하수처리장 분배조 웨어 최적화)

  • Jung, Yong-Jun;Park, Hae-Sik;Cho, Young-Man
    • Journal of Environmental Science International
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    • v.30 no.12
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    • pp.983-991
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    • 2021
  • The role of the distribution basin role is to apportion incoming raw water to the primary sedimentation basin as part of the water treatment process. The purpose of this study was to calculate the amount of water in the distribution basin using computational fluid dynamics (CFD) analysis and to find a way to improve any non-uniformity. We used the Taguchi method and the minitab tool as optimization methods. The results of the CFD calculation showed that the distribution flow had a deviation of 5% at the minimum inflow, 10% at the average inflow, and 22% at the maximum inflow. At maximum flow, the appropriate heights of the 7 weirs(C, D, A, B, E, F, G) were 40 mm, 20 mm, 20 mm, 0, 0, 0, and 20 mm, respectively, according to the Taguchi optimization tool. Here, the maximum deviation of the distribution amount was 9% and the standard deviation was 23.7. The appropriate heights of the 7 weirs, according to the Minitab tool, were 40 mm, 20 mm, 20 mm, 0, 0, 0, and 20 mm, respectively, for weirs C, D, A, B, E, F, and G. Therefore, the maximum deviation of the distribution amount was 8% and the standard deviation was 17.1, which was slightly improved compared to the Taguchi method.