• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5391-5397
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• 2012

Background: Neuroblastoma (NB), like most human cancers, is characterized by genomic instability, manifested at the chromosomal level as allelic gain, loss or rearrangement. Genetics methods, as well as conventional and molecular cytogenetics may provide valuable clues for the identification of target loci and successful search for major genes in neuroblastoma. We aimed to investigate AURKA and MYCN gene rearrangements and the chromosomal aberrations (CAs) to determine the prognosis of neuroblastoma. Methods: We performed cytogenetic analysis by G-banding in 25 cases [11 girls (44%) and 14 boys (66%)] and in 25 controls. Fluorescence in situ hybridization (FISH) with AURKA and MYCN gene probes was also used on interphase nuclei to screen for alterations. Results: Some 18.4% of patient cells exhibited CAs., with a significant difference between patient and control groups in the frequencies (P<0.0001). Some 72% of the cells had structural aberrations, and only 28% had numerical chnages in patients. Structural aberrations consisted of deletions, translocations, breaks and fragility in various chromosomes, 84% and 52% of the patients having deletions and translocations, respectively. Among these expressed CAs, there was a higher frequency at 1q21, 1q32, 2q21, 2q31, 2p24, 4q31, 9q11, 9q22, 13q14, 14q11.2, 14q24, and 15q22 in patients. 32% of the patients had chromosome breaks, most frequently in chromosomes 1, 2, 3, 4, 5, 8, 9, 11, 12, 19 and X. The number of cells with breaks and the genomic damage frequencies were higher in patients (p<0.001). Aneuploidies in chromosomes X, 22, 3, 17 and 18 were most frequently observed. Numerical chromosome abnormalities were distinctive in 10.7% of sex chromosomes. Fragile sites were observed in 16% of our patients. Conclusion: Our data confirmed that there is a close correlation between amplification of the two genes, amplification of MYCN possibly contributing significantly to the oncogenic properties of AURKA. The high frequencies of chromosomal aberrations and amplifications of AURKA and MYCN genes indicate prognostic value in children with neuroblastomas and may point to contributing factors in their development.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.8
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• pp.857-864
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• 2010

In this paper, we analyse the effect of the path imbalances(gain and phase mismatches) in LINC(LInear amplification with Nonlinear Component) system, and propose a simple scheme using LUTs(Look Up Table) to compensate the path imbalances. The EVM(Error Vector Magnitude) and ACPR(Adjacent Channel Power Ratio) of the LINC system are degraded significantly by the path imbalances because it adopts an outphasing technique. The EVM and ACPR are theoretically extracted for two variables(gain and phase mismatch factors) and 2-D LUTs for those are generated based on the analysis. The efficient and simple compensation scheme for the path imbalances is proposed using the 2-D LUTs. A LINC system with the suggested compensation scheme is implemented, and the proposed method is verified with an experiment. A 16-QAM signal with 1.5 MHz bandwidth is used. Before the compensation, the path gain ratio was 95 % and phase error was $19.33^{\circ}$. The proposed scheme adjusts those values with 99 % and $0.5^{\circ}$, and improves ACPR about 18.1 dB.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.3A
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• pp.163-172
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• 2011

The CFTA girder developed is a concrete filled steel tubular system with arched shape and external post-tensioning (PT) tendons which control the initial camber and the bending stress of the girder. In the present study the effects of the PT tendons on the dynamic behavior of the girder subjected to a moving vehicle load are numerically investigated. Various levels for the tendon quantity and the tendon forces are considered, using the existing FE model of the girder. The vehicle considered is a DB-24 truck and is modeled with two tracks-three axles. Equivalent-load pulse time histories are applied to each node to simulate the moving vehicle, depending on the time of arrival and the discretization. The vehicle speeds are varied from 40 km/hr to 100 km/hr with increment of 20 km/hr. The analysis results show that the tendon forces do not produce any influences on the dynamic responses of the girder. However the dymamic deflection of the girder increases when a smaller amount of tendons is used. The Dynamic Amplification Factors (DAF) are evaluated based on the static and dynamic responses. Much lower values of the DAF are obtained, even no tendons applied, than those provided by the design criteria of the AASHTO LRFD and the Korea Highway Standard Specification.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2745-2749
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• 2015

Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province. Materials and Methods: A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 gene polymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysis of the data. Results: The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases, accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportion between negative and positive family history of patients was statistically significant, ${\chi}^2=6.213$, p=0.045. There was no statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking, ${\chi}^2=0.119$, p=0.998, and ${\chi}^2=1.727$, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes (AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salt taste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05). There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1 gene. Conclusions: The PLCE1 rs2274223 polymorphism has a relationship with family history of esophageal cancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene, eating habits, living around the environment and occupation in cases.

• Parasites, Hosts and Diseases
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• v.54 no.5
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• pp.645-652
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• 2016

Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8-24.9%), 75 (17.2; 13.8-21.1), 29 (40.9; 29.3-53.2), and 5 (7.6; 2.5-16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported.

• Journal of Korean Society of Steel Construction
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• v.15 no.2
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• pp.197-206
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• 2003

The optimal design method in cooperation with a nonlinear elastic analysis method was presented. The proposed nonlinear elastic method overcame the drawback of the conventional LRFD method this approximately accounts for the nonlinear effect caused by using the moment amplification factors of and. The genetic algorithm uses a procedure based on the Darwinian notions of the survival of the fittest, where selection, crossover, and mutation operators are used to look for high performance among the sections of the database. They satisfy constraint functions and give the lightest weight to the structure. The objective function was set to the total weight of the steel structure. The constraint functions were load-carrying capacities, serviceability, and ductility requirement. Case studies for a two-dimensional frame, a three-dimensional frame, and a three-dimensional steel arch bridge were likewise presented.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.32 no.2
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• pp.112-117
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• 2010

Aurora kinases represent a novel family of serine/threonine kinases crucial for cell cycle control. Aurora-2 kinase is mainly involved in centrosome function, mitotic entry, and spindle assembly. Aurora-2 kinase overexpression causes centrosome amplification and the formation of multipolar mitotic spindles, which leads to tumor aneuploidy and so it has been found to play an important role in tumorigenicity in many cancers such as colorectal cancer, breast cancer and cervical cancer. Hence, the goal of this study is to identify the correlation of clinicopathlogical factors and overexpression of Aurora-2 kinase in oral squamous cell carcinoma. We studied the immunohistochemical staining of Aurora-2 kinase in 20 specimens of 20 patients with oral squamous cell carcinoma and the relationships between Aurora-2 kinase over expression and each of the clinico-pathological parameters were analyzed by Pearson correlation analysis. Statistical significance was set at P < 0.05. The results were as follows. 1. In the immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, the high level staining of Aurora-2 kinase was observed. 2. The correlation between immunohistochemical Aurora-2 kinase expression and histopathological differentiation of specimens was significant. These findings suggest that overexpression of Aurora-2 kinase may play a important role in carcinogenesis of oral squamous cell carcinoma.

• The Journal of the Korean Society for Microbiology
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• v.34 no.6
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• pp.513-518
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• 1999

A total of 113 Vibrio sp. strains were examined for plasmid content which were subjected to digestion with restriction enzymes. About the 55% Vibrio spp. have the plasmid more than one. Most of these plasmid various derivatives ranged from $2.4\;kb{\sim}23\;kb$, especially two strains of V. mimicus and one strain of V. furnissii carried one high-molecular weight plasmid (molecular weight ranging between $70\;kb{\sim}100\;kb$). Results of restriction analysis for plasmid of this three strains were by no means the rule. For detection of tdh and ctx gene, the virulence factor involved in the pathogenesis, we carried out the TDH and CT assay, PCR amplification, and hybridization. A total 11 strains were produced TDH, involved in 9 strains of V. parahaemolyticus and 1 strain of V. alginolyticus from clinical isolates and 1 strains of V. mimicus from environmental isolates. In the experiments of tdh gene detection, in all, 3 strains of V. parahaemolyticus from clinical isolates and 2 strains from environmental isolates could be successfully amplified in 400 bp by PCR. The PCR results were consistent with DNA hybridization tests. In the experiments of CT assay, in all, 3 strains of V. cholerae from clinical isolate and 1 strain of V. cholerae from environmental isolates were observed CT-producing. These CT-producing strains amplified in 302 bp by PCR for the detection of ctx gene. All CT-producing strains hybridized with digoxigenin-labeled DNA probe, while CT-negative strains did not hybridize. Also hybridization tests results for detection of ctx gene consistent with PCR.

• The Journal of the Korea Contents Association
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• v.10 no.7
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• pp.23-30
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• 2010

It is important for the strategy of cosmetic sales to investigate the sensibility and the preference degree in the environment that the makeup style has been changed focusing on the consumer center. We proposed the human sensibility ergonomics makeup recommendation system (MakeupRS) using the context sensor information applying the collaborative filtering technique as one of methods in the makeup style development centered on the consumer's sensibility and the preference. In the collaborative filtering technique, the Pearson correlation coefficient applying to the case amplification is used to calculate similarity weights between the users. To investigate the sensibility according to the effect of makeup styles, the makeup styles were analyzed in terms of 6 style factors, such as, the foundation, the color lens, the eye shadow, the eye lash, the cheek brusher, and the lipstick. Ultimately, this paper suggests empirical application to verify the adequacy and the validity with the human sensibility ergonomics makeup recommendation system.

• Journal of Navigation and Port Research
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• v.27 no.5
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• pp.519-526
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• 2003

For preliminary structural analysis of superstructures on very large floating structures(VLFS), superstructures are analyzed considering elastic deformations of barge type lower-structures subjected to wave loads. In this case, to consider the effect of wave loads on the superstructure, initial displacements at the support points of superstructures are evaluated as input data for the analysis. However, the evaluation and application of displacement loads are tedious and very time-consuming processes. Therefore, this paper proposes a simplified static analysis method to analyze the structural behaviors of superstructures on very large floating structures subjected to wave loads. In this study, the member forces due to the variation of beam span and the amplitude and period of wave load are analyzed by using an example 4 span -3 story structure and the amplification factors for beam moments are represented by the specific regression equation.