• Title/Summary/Keyword: ammonium nitrate

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Pseudomonas oleovorans의 유가식 배양에 의한 medium chain length Polyhydroxyalkanoates (MCL-PHA) 생산

  • Kim, Beom-Su;Im, Hui-Yeon
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.207-210
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    • 2000
  • Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHA) fram octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat fed-batch culture technique. The octanoate concentration of the culture broth was maintained below 4 g/L by feeding the mixture of octanoic acid and ammonium nitrate when the culture pH rose above high limit. The effect of the ratio of octanoic acid to ammonium nitrate (C/N ratio) in the feed on the PHA production was examined. The final cell concentrations of 62.5, 54.7, and 9.5 g/L, PHA contents of 62.9, 75.1, and 67.6% of dry cell weight, and productivities of 1.03, 0.632, and 0.161 g/L/h were obtained when the C/N ratio in the feed were 10, 20, and 100 g octanoic acid/g ammonium nitrate, respectively.

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Ionic Compositions of PM2.5 during Summer and Winter in the Downtown Area of Jeju City in Jeju Island (제주시 도심지역에서 여름과 겨울의 PM2.5 이온조성 특성)

  • Lee, Ki-Ho;Hu, Chul-Goo
    • Journal of Environmental Science International
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    • v.26 no.4
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    • pp.447-456
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    • 2017
  • Chemical properties of aerosols were investigated by analyzing the inorganic water-soluble content in $PM_{2.5}$ collected in the downtown area of Jeju City in Jeju Island. Due to an increase in both the number of visiting tourists and the size of local population, the number of cars in this area is increasing, causing an increase in $PM_{2.5}$. Eight $PM_{2.5}$-bound major inorganic ions were analyzed during the summer and winter periods. The water-soluble inorganic component represents a significant fraction of $PM_{2.5}$. In particular, secondary inorganic aerosols contribute 36.2% and 47.5% of $PM_{2.5}$ mass in summer and winter, respectively. Nitrate concentrations increase for $[NH_4{^+}]/[SO_4{^{2-}}]$>1.5, and excess ammonium, which is necessary for ammonium nitrate formation, is linearly correlated with nitrate. These results are clearly observed during the winter because conditions are more conducive to the formation of ammonium nitrate. A significant negative correlation between Nitrogen Oxidation Ratio (NOR) and temperature was observed. The obtained results can be useful for a better understanding of the aerosol dynamics in the downtown area in Jeju City.

Preparation of Calcium Carbonate with High Purity by using Ammonium Chloride Process and Ammonium Nitrate Process (Ammonium Chloride Process 및 Ammonium Nitrate Process를 이용한 고순도 탄산칼슘의 제조)

  • Kang Mee-Sook;Soh Gowan-Soon;Shin Dong-Hwa
    • Journal of Food Hygiene and Safety
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    • v.19 no.4
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    • pp.203-208
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    • 2004
  • The shells of Anadarac tegillarca granosa, Crassostrea gigas, Crassostrea nippona, and Patinopecten yessoensis were used for preparation of calcium carbonate with high purity. Calcium content in ash of shell was the highest $64.9\%$ in Anadarac tegillarca granosa ashed for 5 hr at $900^{\circ}C$, and followed as Patinopecten yessoensis $62.5\%$, Crassostrea gigas $62.4\%$, and Crassostrea nippona $61.5\%$. Whiteness of ash was the highest 81.6-85.8 in Patinopecten yessoensis shell. Calcium contents in calcium carbonates made with shells of Anadarac tegillarca granosa by using ammonium chloride process (ACP) and ammonium nitrate process (ANP) were higher $40.03-40.04\%$ than $39.92\%$ in Anadarac tegillarca granosa ash. Calcium content was the highest $40.04\%$ in pH adjusted calcium carbonate prepared by using ANP. Whiteness of calcium carbonate prepared by using ACP and ANP was the level of 101.0-101.5. Therefore, we estimated that the calcium carbonate made by using ACP and ANP could be used potentiality as a food additive for calcium supplement.

Effects of Nitrogen Addition on Soil Respiration (상수리나무림 임상에 공급한 무기질소가 토양호흡에 미치는 영향)

  • 최주섭;문형태
    • The Korean Journal of Ecology
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    • v.27 no.3
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    • pp.155-159
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    • 2004
  • In order to gain a better understanding of how forests participate in the cycling of carbon, effects of nitrogen addition on soil respiration were investigated on the oak forest in Kongju, Korea. Study site was divided into control, treatment f and treatment 2 plots, with 5 replication in each plot. In each replicate of treatment 1 and treatment 2 were fertilized with ammonium nitrate (NH$_4$NO$_3$), 30 g/$m^2$ and 60 g/$m^2$, respectively. Soil respiration, soil temperature, ammonium-N and nitrate-N were measured during the experimental period. Ammonium-N and nitrate-N in Ta were higher than those in control and T$_1$. Ammonium-N and nitrate-N in top-soil and sub-soil decreased sharply in August after bi9 rainfall in July in T$_1$ and T$_2$, however, those in control plot increased. Soil respiration in T$_2$ Plot showed consistently higher than those in control and T$_1$ until the end of July. However, soil respiration was similar among the control, T$_1$ and T$_2$ in mid-August and September The amount of Co$_2$ released from soil respiration in control, T$_1$ and T$_2$ in mid-August was 8.0$\pm$0.4, 9.3$\pm$0.6 and 10.2$\pm$0.5 $\mu$mol$^{-1}$ ㆍm$^{-2}$ ㆍs$^{-1}$ , respectively. However, those in control, T$_1$ and T$_2$in mid-August was 13.0$\pm$0.4, 13.5$\pm$0.5, 13.3$\pm$0.6 $\mu$mol$^{-1}$ ㆍm$^{-2}$$^{-1}$ , respectively. The results suggest that nitrogen addition in this oak forest has a positive effect on soil respiration.

Effects of Urea, Ammonium Nitrate and Calcium Silicate on the Absorption, Translocation of Potassium and Yield of Chinese Cabbage (Brassica Perkinnensis, Var. Samjin) (요소, 질산암모늄 및 규회석분말이 칼리의 흡수 및 배추의 수량에 미친 영향)

  • Oh, Wang-Keun;Kim, Jae-Young;Kim, Sung-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.4
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    • pp.337-340
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    • 1987
  • This experiment was conducted on relatively acid sandy loam soil at Choong-ju campus, Kon-kuk University aimed at clarifying the influence of urea and ammonium nitrate on the effect of potassium to Chinese cabbage in soils either limed or unlimed with calcium silicate. The results are summarized as follows: a. Calcium silicate application enhanced cabbage growth and under this condition, the difference in effect of urea and ammonium nitrate can hardly be observed. b. Without calcium silicate application, the response of Chinese cabbage to ammonium nitrate was more distinctive than that to urea. This was partially attributable to the greater use of soil born potassium at ammonium nitrate treatment. c. Added potassium was not only affective in increasing cabbage yield but also contributed in improving quality of cabbage by producing greater edible portion of the cabbage. Such K effect was particularly pronounced on the acid soil where calcium silicate application was neglected. d. Potassium was easily translocated from outer leaves to inner leaves and thus, the concentration of K content in outer leaves played as a limiting factor of cabbage yield. Less than 20 me/100gr of K content in harvested dried outer leaves resulted in a linear reduction of cabbage yield.

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The Effects of Ammonium Citrate and Ammonium Succinate on the Growth of Cells and Nitrogen Absorption in Korean Ginseng Suspension Cultures (고려인삼(Panax ginseng C.A. Meyer) 배양세포의 생육 및 질소 흡수에 미치는 Ammonium Citrate와 Ammonium Succinate의 영향)

  • 김홍성;김명원;소상섭;강영희
    • Journal of Plant Biology
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    • v.24 no.1
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    • pp.13-19
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    • 1981
  • In order to investigate the effects of ammonium citrate and ammonium succinate on the growth and absorption of nitrogen compounds supplied in the medium, Korean ginseng (Panax ginseng C. A. Meyer) calli were suspension cultured in MS medium with various concentrations of ammonium citrate and ammonium succinate. When Korean ginseng calli were cultured with 10 mM ammonium citrate, 10 mM ammonium succinate, and 10 mM ammonium nitrate (control) in MS media as the nitrogen sources, the growth, $NO_3$-N absorption and total nitrogen content of the Korean ginseng cells were greatest in the ammonium citrate and ammonium succinate concentrations. When Korean ginseng calli were cultured with 5 mM ammonium citrate and 5 mM ammonium succinate, the growth and nitrogen content were superior to those of the control: however, $NO_3$-N and $NH_4$-N absorptions were similar to those of the control. In conclusion, the 10 mM ammonium citrate and 10 mM ammonium succinate may be better able to facilitate the growth and $NO_3$

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Nitrate Reduction without Ammonium Release using Fe-loaded Zeolite

  • Lee Seunghak;Lee Kwanghun;Lee Sungsu;Park Junboum
    • Journal of Soil and Groundwater Environment
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    • v.10 no.1
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    • pp.1-5
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    • 2005
  • Nitrate reduction with zero valent iron $(Fe^0)$ has been extensively studied, but the proper treatment for ammonium byproduct has not been reported yet. In groundwater, however, ammonium is regarded as contaminant species, and particularly, its acceptable level is regulated to 0.5 mg-N/L. for drinking water. This study is focused on developing new material to reduce nitrate and properly remove ammonium by-products. A new material, Fe-loaded zeolite, is derived from zeolite modified by Fe(II) chloride followed by reduction with sodium borohydride. Batch experiments were performed without buffer at two different pH to evaluate the removal efficiency of Fe-loaded zeolite. After 80 hr reaction time, Fe loaded zeolite showed about $60\%$ nitrate removal at initial pH of 3.3 and $40\%$ at pH of 6 with no ammonium release. Although iron filing showed higher removal efficiency than Fe-loaded zeolite at each pH, it released a considerable amount of ammonium stoichiometrically equivalent to that of reduced nitrate. In terms of nitrogen species including $NO_3-N$ and $NH_4^+-N$, Fe-loaded zeolite removed about $60\%\;and\;40\%$ of nitrogen in residual solution at initial pH of 3.3 and 6, respectively, while the removal efficiency of iron filing was negligible.

A Study on Nitrogen Metabolism of Lemnaceae: Assimilation of Nitrate and Ammonia in Spirodela polyrhiza and Lemna aequinoctialis (개구리밥과 식물의 질소대사에 관한 연구: 개구리밥(Spirodela polyrhiza)과 좀개구리밥(Lemna aequinoctialis)의 NO3-와 NH4-의 동화작용)

  • 장남기
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.253-260
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    • 1991
  • Spirodela polyrhiza and Lemna aequinoctialis often occurred at the sites of high ammonium concentration and at the sites of high nitrate concentration, respectively. We investigated the different distribution between two species in relation to the type of nitrogen sources and their concentrations. Our experiments showed that L. aequinoctialis grew faster than S. polyrhiza in nitrate media with lower than 15 mM concentration. The nitrate uptake was also faster in L. aequinoctialis than in S. polyrhiza. However, neither differences in growth nor in uptake patterns between these two species were observed in ammonium media. Glutamine synthetase (GS), glutamate dehydrogenase (GDH) and glutamate synthetase (GOGAT) activities were higher in L. aequinoctialis. In particular, nitrate reductase activity (NRA) in L. aequinoctialis was 12.1 times as high as that in S. polyrhiza. These results showed that the two species responded varyingly to the types of nitrogen sources and their concentrations. Therefore, the difference in geographic distribution between the two species appeared to reflect the interspecific differences in enzyme activities and, subsequently, nitrogen absorption abilities.

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Effect of nitrogen sources on production of exopolymer by Pseudomonas elodea ATCC 31461

  • Lee, Nam-Gyu;Kim, Hyeon-Suk;Seo, Hyeong-Pil;Jeong, Dae-Yeong;Kim, Ji-Mo;Lee, Jin-U
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.247-250
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    • 2000
  • Extracellular polysaccharide was produced by Pseudomonas elodea ATCC 31461 under aerobic condition. Nitrogen sources in medium affected cell growth and production of exopolymer. Ammonium nitrate limitation was found to be essential for higher production of exopolymer. Conversion rate of exopolymer from glucose under ammonium nitrate limitation was about 5 times higher than with ammonium nitrate.

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Quantification of Sampling Artifacts in PM2.5 Inorganic Ion Species using Teflon Filter (테플론 여과지를 이용한 미세입자 무기이온 성분 측정에서의 오차 정량)

  • Kim, Jung-Youn;Kim, Yong-Pyo
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.1
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    • pp.74-83
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    • 2007
  • Artifacts due to inter-particle and gas-particle interactions during PM2.5 sampling were quantified by comparing the measurement results between the annular denuder-filterpack system and the filterpack system without denuder. Measurements were carried in Seoul for 10 days in each season; Nov. 2004, Jan. 2005, Mar. 2005, and Jul. 2005, respectively. In each day, two 12-h samples were obtained. The concentrations of nitrate and chloride showed seasonal variations mainly due to the availability of ammonium to neutralize nitrate or chloride. Nitrates and chloride losses were prominent in summer. Since most of ammonia was used to neutralize sulfuric acid and formed ammonium sulfate in summer, nitrate and chloride could not exist in particles and ammonium loss was smaller than other seasons.