• KSBB Journal
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• v.11 no.3
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• pp.329-339
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• 1996

The effects of ammonium ion on cell growth kinetics, monoclonal antibody productivity, and cell metabolism of hybridoma cells were investigated. The mouse-mouse hybridoma cell line VlIIH-8 producing mouse IgG2a was used as a model system. Ammonium ion showed an inhibitory effect on cell growth and monoclonal antibody production. New immobilized adsorbents were developed for the reduction of the inhibitory effect of ammonium ion. The ammonium ion selective zeolite, Phillipsite-Gismondine was entrapped in calcium alginate bead or in dialysis membrane and applied to the hybridoma cell culture system for the in situ removal of ammonium ion from culture media. The effects of ammonium the both serum supplemented and serum free media on the cell growth were studied by applying immobilized adsorbents of calcium alginate bead type. The results demonstrated a substantial enhancement in cell growth. Applying immobilized adsorbents of dialysis membrane type to serum supplemented media also resulted in the stimulation of cell growth, cell viability and monoclonal antibody production.

• KSBB Journal
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• v.10 no.2
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• pp.170-175
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• 1995

The effect of nitrogen source on production of a high viscosity exopolysaccharide, methylan, from methanol by Mentylobacterium organophilum was investigated in fed-batch culture. During the fermentation, cells continued to grow even after the nitrogen source added to the medium was depleted and methylan production was stimulated under the condition which ammonium ion was depleted. Cell growth increased proportionally to the initial concentration of ammonium ion in the medium, but methylan production was significantly inhibited at the high concentration of ammonium ion. As the initial concentration of ammonium ion increased, the specific growth rate, the specific product formation rate and the specific substrate consumption rate decreased due to the inhibitory effect of excess ammonium ions. In order to reduce the inhibitory effect by high concentration of ammonium ion. The control of ammonium ion concentration within the desired level(usually $0.45g/\ell$) was necessary. When ammonium ion concentration was maintained below $0.15g/\ell$ by exponential feeding, methylan production could be increased up to $12.5g/\ell$.

• Korean Journal of Microbiology
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• v.6 no.1
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• pp.29-35
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• 1968

There are many reports on the effect of salts to the fermentation of the yeasts, but there are a few reports on the effect of the ammonium thiocyanate(NH$_4CNS$) on the fermentation of the yeasts(Saccharomyces brenerei-Hefe-Rasse X11, Saccharomyces formosensis No. 396 IAM and Saccharomyces cerevisiae IAM). A study has been made on the effects of the ammonium thiocyanate on the fermentation of the above yeasts. The results of the research are as belows: 1) The addition of 0. 00001 mol. of ammonium thiocyanate makes the fermentation of the yeast(Sacch. formosensis No. 39 IAM) facilitate, but has not influenced the another yeast (Sacch. brenerei-Hefe-Rasse XII). 2) The addition of 0. 001 mol. of ammonium thiocyanate makes fermentation of Rases XII the fastest, but the on other yeast (Sacch. formosensis No - 396 IAM) was accelerated by the addition of 0. 1 mol., and it seems to have abnormal fermentation by the addition of 0. 0001 mol. The addition of ammonium thiocyanate(0. 00001-0. 001 mol.) inhibited the fermentatirn of the yeast(Sacch. cerevisiae IAM), but the concentration of 0. 1 mol. does not interrupt the fermentation of Sacch. cerevisiae IAM and increased the fermentation. 3) The order of effects to the fermentation of each yeast is Sacch. brenerei-Hefe-Rasse XII, Sacch. formosensis No. 396 IAM, Sacch. cerevisiae IAM.

• Journal of Korean Society of Water and Wastewater
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• v.11 no.4
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• pp.43-53
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• 1997

In this research, the synthetic livestock wastewater was prepared to study the characteristics of organic matter removal, the change of VFA production, and the amount of gas production with respect to the change of ammonium nitrogen concentration in the waste using anaerobic fixed bed process, which is an anaerobic biofilm process. The HRT and operation temperature were 1 day and $35{\pm}1^{\circ}C$, respectively. Also, the characteristics of organic matter removal and the inhibitory effect on microorganism in the anaerobic process were studied on the organic loading and ammonium nitrogen concentration. The results obtained were as follows: For COD loading of $10kg/m^3$-day and five levels of ammonium nitrogen concentration ranging from 1,000 to 5,000 mg/L, organic removal efficiencies were about 81, 74, 67, 58, and 51%, and gas productions were 3,860, 3,520, 3,240, 3,020, and 2,790 ml/l-day, respectively. Average methane contents in the gas produced on COD loading of $10kg/m^3$-day was about 76%. Throughout the whole period of experiment, remaining VFA (as COD base) in the effluent was over 90% of remaining COD. This result indicated the inhibitory effect of high concentration of ammonium nitrogen through the facts that accumulated VFA was almost COD and organic removal efficiency decreased also with the increase of ammonium nitrogen. Especially, that implys which high concentration of ammonium nitrogen not only inhibits methane forming bacteria, but also acid forming bacteria.

• Microbiology and Biotechnology Letters
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• v.19 no.2
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• pp.186-192
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• 1991

It is very important to have a good kinetic model which considers the effects of both ammonium and glucose for the control and optimization of the poly-${\beta}$-hydroxybutyrate (PHB) fermentation. A kinetic model for the growth of Alcaligenes eutrophus and the biosynthesis of PHB under both ammonium and glucose limitation was proposed. Growth rate of residual biomass was expressed as a function of concentrations of residual biomass, glucose and ammonium having glucose inhibition. PHB production rate was expressed as a function of concentrations of residual biomass, glucose, ammonium and PHB content having ammonium and product inhibitions. Novel approaches were made to estimate the parameters in the model equations which considered two limiting substrates. Model parameters were evaluated by graphical and simplex methods. The proposed kinetic model fitted the data very well.

• Asian Journal of Turfgrass Science
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• v.4 no.1
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• pp.24-30
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• 1990

This study was carried out to search for growth and quality -of turfgrass by Nitrogen source (Urea Ammonium sulphate) and organic matter, and to obtain methods of pr6per turfgrass management by the results. This experiment was conducted in turf field from 1988 to 1989. The results of this experiment was summarized as follows; 1.Without application of organic matter, turfgrass growth between urea and ammonium sulphate was not significant, however ammonium sulphate resulted in superior to urea in total dry weight when organic matter was applied. 2.The addition of organic matter showed significant effect on color rating and visible quality, and ammonium sulphate was higher quality than urea. 3.Shoot density increased, particularly in ammonium sulphate treatment, when organic matter was applied. 4.Rhizome and stolon were shown significant effect in urea treatment by the application of organic matter. 5.Surface soil pH was allowed to become slightly acid by the ammonium sulphate treatments, but soil acidity remained mostly unaffected when organic matter was supplied. 6.Total nitrogen of log tissue and organic matter content increased when organic matter w-as applied.

• KSBB Journal
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• v.11 no.5
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• pp.613-621
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• 1996

The effects of important medium components such as carbon, nitrogen sources and amino acids on the production of cyclosporin A(CyA) were investigated in an immobilized fungal cell fermentation using Tolypocladium inflatum. As carbon sources in the synthetic medium, fructose and maltose stimulated CyA production remarkably compared to glucose when ammonium sulfate was supplemented as a nitrogen source. In the absence of ammonium sulfate in the medium, however, CyA biosynthesis was reduced considerably without regard to C-sources tested. Ammonium sulfate was found to be the best N-source, and also ammonium phosphate and ammonium citrate showed some positive effects on CyA production. Optimum concentration of ammonium sulfate was 10g/L, and supplementation of ammonium sulfate at the start of fermentation was found to be the most efficacious for maximal production of CyA. Among the constituent amino acids of cyclic peptide, CyA, L-valine had the most significant effect on the biosynthesis of CyA, and maximum CyA production was observed when 10 g/L of L-valine was initially added.

• Journal of the Korean Applied Science and Technology
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• v.27 no.4
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• pp.399-406
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• 2010

This study was performed to test the flame retardancy of zelkova sarrata-based materials by the treatment of ammonium salts. Zelkova sarrata plate was soaked by the treatment with three 20 wt% ammonium salt solutions consisting ammonium chloride (AMSL), monoammonium phosphate (MAPP), and diammonium phosphate (DAPP), respectively, at the room temperature. After the drying specimen treated with chemicals, combustion properties were examined by the cone calorimeter (ISO 5660-1). When the ammonium salts were used as the retardant for zelkova sarrata, the flame retardancy improved due to the treated ammonium salts in the virgin zelkova sarrata. However the specimen shows increasing CO over virgin zelkova sarrata and It is supposed that toxicities depend on extents. Also, the specimen with ammonium salts showed the higher total smoke release (TSR) than that of virgin plate. Of specimens treated with ammonium salts the ammonium chloride handled the test side was considered a improved inhibitory effect of combustion.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.219.1-219.1
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• 2011

These studies carried out to know the effect of ammonium on the current generation in the microbial fuel cells (MFCs). MFCs used in the study were enriched with anaerobic digestion sludge and operated for 3 years using artificial wastewater (AWW). When the current was stably generated, ammonium ion with $27.0{\pm}0.0$, $51.5{\pm}0.0$, $103.5{\pm}0.0mg/L$ with acetate fed into the anode compartment. The current values under condition included ammonium were changed from its initial $6.30{\pm}0.06$ to $6.28{\pm}0.36$, $5.95{\pm}0.61$, $5.64{\pm}0.38mA$, respectively. The current value was slightly decreased to $5.64{\pm}0.38mA$ compared to $6.30{\pm}0.06mA$ generated from MFC without ammonium ion in the AWW. But After 3days operating under ammonium concentration with $103.5{\pm}0.0mg/L$, the current was unstably generated when artificial wastewater without ammonium was fed again. MFC enriched with AWW without ammonium ion was inhibited by high concentration of ammonium. At this time, the ammonium was removed 5.27~16.41 mg per day under all conditions.

• KSBB Journal
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• v.13 no.4
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• pp.404-410
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• 1998

Three types of adsorbents were developed by immobilizing synthetic zeolite, Philipsite-Gismonine, in alginate, cellulose acetate and dialysis membrane for the in situ removal of ammonium ion which inhibits growth and productivity of animal cells such as CHO cells producing tPA. Ammonium ion removal efficiency and cell growth promoting effect with various immobilized adsorbents were evaluated and the membrane type was selected as an optimal immobilized adsorbent. The experiments were then simulated by adding 8mM ammonium chloride and immobilized adsorbent in order to validate the removal effect under high density cell cultures. The results showed increase in maximum cell density by three times, in cell viability, and in tPA productivity by 40%. And it was found that the promoting effects were more significant in case of high ammonium ion concentration system. It was also found that the optimum addition time for immobilized adsorbents was 48 hr in the absence of ammonium chloride addition and 72 hr in the presence of ammonium chloride addition.