• 자원리싸이클링
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• 제13권4호
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• pp.32-38
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• 2004

중유의 대체연료로서 관심을 받고있는 오리멀젼회로부터 바나듐을 회수하기 위한 기초연구로서 오리멀젼회의 물성과 침출특성에 관한 연구를 수행하였다. 오리멀젼회는 16%의 바나듐, 4%의 니켈 그리고 9%의 황을 함유하고 있으며 $d_{50}$이 5.9$mu extrm{m}$로 미세한 분말이었다. 오리멀젼회 중의 금속성분이 황산염 형태로 존재하기 때문에 바나듐은 수 침출이 용이하고 침출시간도 10분 이하로 짧았다. 수침출 시 반응온도가 높으면 침출율이 감소하는데 이는 V(V)이 가수분해되어 $V_2$$O_{5}$로 침전되기 때문이다. 한편 황산을 첨가하면 바나듐의 침출율을 높일 수 있었다. 바나듐의 선택적 침출을 위한 알카리 침출의 경우 침출율을 높이기 위하여는 산화제의 사용이 필요하며 과산화수소가 적당하였다.

• 한국축산식품학회지
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• 제39권4호
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• pp.601-609
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• 2019

Bifidobacterium longum KACC 91563 secretes family 5 extracellular solute-binding protein via extracellular vesicle. In our previous work, it was demonstrated that the protein effectively alleviated food allergy symptoms via mast cell specific apoptosis, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, we cloned the gene encoding extracellular solute-binding protein of the strain into the histidine-tagged pET-28a(+) vector and transformed the resulting plasmid into the Escherichia coli strain BL21 (DE3). The histidine-tagged extracellular solute-binding protein expressed in the transformed cells was purified using Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the process, two liters of transformed culture produced 7.15 mg of the recombinant proteins. This is the first study describing the production of extracellular solute-binding protein of probiotic bacteria. Establishment of large-scale production strategy for the protein will further contribute to the development of functional foods and potential alternative treatments for allergies.

• 한국축산식품학회지
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• 제43권3호
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• pp.428-440
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• 2023

Global meat consumption is increasing worldwide, however, supply remains lacking. Several alternative protein sources, such as cultured meat, plant-based protein production, and edible insects, have been proposed to overcome this shortage. Interestingly, edible insects are characterized by superior digestive and absorptive qualities that make them the ideal replacement for traditional protein production. This study aims to further the processing ability of insect protein by investigating the effects of various pre-treatment methods, such as blanching (HB), roasting (HR), and superheated steam (HS), on the nutritional properties and physicochemical characteristics of proteins extracted from Hermetia illucens larvae. The drying rate, pH value, color analysis, amino and fatty acid profile, as well as bulk density, shear force, and rehydration ratios of the above pre-treatment methods, were explored. HS was found to have the highest drying rate and pH value analysis showed that HB and HS samples have significantly higher values compared to the other modalities. Raw edible insects had the highest value in the sum of essential amino acid (EAA) and EAA index when compared to EAAs. HB and HS showed significantly lower bulk density results, and HS showed the highest shear force and the highest value in rehydration ratio, regardless of immersion time. Therefore, taking the above results together, it was found that blanching and superheated steam blanching pre-treatment were the most effective methods to improve the processing properties of H. illucens after hot-air drying.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.6-6
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• 2023

Nosemosis is one of the most common protozoan diseases of adult bees (Apis mellifera). Nosemosis is caused by two species of microsporidia; Nosema apis and Nosema ceranae. Nosema ceranae is potentially more dangerous because it has the ability to infect multiple cell types, and it is now the predominant microsporidian species in A. mellifera. In this study, we identified two anti-nosemosis plants, Aster scaber and Artemisia dubia, which reduced the spore development of N. ceranae in spore-infected cells. We intend to establish the anti-nosemosis activity of aqueous, ethyl acetate (EA), and butanol (BuOH) extracts of A. dubia and A. scaber. In order to determine the optimal dose, we did in vitro and in vivo toxicity for all the extracts and carried out anti-nosemosis experiments. Although all of the extracts (aqueous, EA, and BuOH) showed in vitro and in vivo anti-nosemosis activity in a dose-dependent manner, the aqueous extracts of A. dubia and A. scaber showed more potent anti-nosemosis activity than the EA and BuOH extracts. And then, we isolated five phenolic compounds [chlorogenic acid, 3,4-dicaffaeoylquinic acid (3,4-DCQA), 3,5-dicaffaeoylquinic acid (3,5-DCQA), 4,5-dicaffaeoylquinic acid (4,5-DCQA), and coumarin] from A. dubia, A. scaber, and A. dubia + A. scaber aqueous extracts and screened for their toxicities and anti-Nosema effects in both in vivo and in vitro conditions. Among these five compounds, coumarin, chlorogenic acid, and 4,5-DCQA exhibited less toxic but more potent anti-Nosema effects than the other two compounds. Especially, chlorogenic acid and coumarin showed prominent anti-Nosema activities even at the lowest concentration (10 ㎍/mL). They might have potential to be developed as alternative compounds for the control of Nosema disease.

• 한국축산식품학회지
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• 제44권2호
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• pp.356-371
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• 2024

Novel meat-inspired products, such as cell-cultivated meat and meat analogues, embrace environmental sustainability, food safety and security, animal welfare, and human health, but consumers are still hesitant to accept these products. The appearance of food is often the most persuasive determinant of purchasing decisions for food. Producing cultivated meat and meat analogues with similar characteristics to conventional meat could lead to increased acceptability, marketability, and profitability. Color is one of the sensorial characteristics that can be improved using color-inducing methods and colorants. Synthetic colorants are cheap and stable, but natural pigments are regarded as safer components for novel food production. The complexity of identifying specific colorants to imitate both raw and cooked meat color lies in the differences in ingredients and methods used to produce meat alternatives. Research devoted to improving the sensorial characteristics of meat analogues has noted various color-inducing methods (e.g., ohmic cooking and pasteurization) and additives (e.g., lactoferrin, laccase, xylose, and pectin). Additionally, considerations toward other meat components, such as fat, can aid in mimicking conventional meat appearance. For instance, the use of plant-based fat replacers and scaffolds can produce a marked sensory enhancement without compromising the sustainability of alternative meats. Moving forward, consumer-relevant sensorial characteristics, such as taste and texture, should be prioritized alongside improving the coloration of meat alternatives.

• 유통과학연구
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• 제13권7호
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• pp.63-71
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• 2015

Purpose - The characteristics of the modern management environment in Korea clearly involve the limitations of knowledge management, globalization, changes in industrial structures, polarization of the economy, consumption, and education, and the demand for creative management. To achieve a continuous competitive advantage for individuals as well as organizations, Wisdom Management can be said to be a paradigm for the current environment. The Research is an alternative to environmental management in the early study of the need to conceptualize the theoretical approach to wisdom management. In other words, it presents a new management paradigm for a business environment of uncertainty and unpredictability. Furthermore, it aims to propose Wisdom Management as an alternative in the modern management environment as well as a topic of discourse. Its purpose is to provide basic knowledge to people who study and practice in this field to strengthen the competitiveness of individuals and companies. Research design, data, and methodology - This paper first provides six characteristics of the current management environment at home and abroad. It then attempts to emphasize the necessity of wisdom based on such characteristics by classifying wisdom according to recent management study research. This allows the derivation of definitions of wisdom management, suggesting its components and properties. Results - The six characteristics of the modern business environment are: 1) limits of knowledge, 2) intensifying globalized competition, 3) changes in industry structure - IoT (Internet of all Things) trend, 4) serious polarization and social unrest diffusion, 5) personal and organizational competitiveness creativity requirements, and 6) domestic special national and social situations. Therefore, accordingly, wisdom can be defined and classified as: wisdom that offers potential integration beyond knowledge, practicing and maintaining knowledge wisdom as an efficient strategy for an organization wisdom as a decision-making process that addresses ambivalence wisdom as the pursuit of common goods based on ethics; and, wisdom as creativity that involves intuition and insight. This paper attempts to define Wisdom Management in accordance with these definitions of wisdom. Wisdom Management is a management system that is awareof the current environment given in the multidimensional perspective, achieves the efficiency of the organization and the goals of common good through an uncertain decision-making process, based on the organization of individual and organizational knowledge, and the internal experiences and resources. Wisdom Management components include human beings, virtue, knowledge, creativity, and ambivalent thinking. Its properties include intentionality, self-destructiveness, creative and ethical reflexivity, complexity, insight, unpredictability, ambivalence, and wholeness. Conclusions - Since all organizations face the limitations of their environment, crises, and opportunities, applying wisdom in management to develop as Wisdom Management can be an inevitable alternative to the uncertainties of the management environment faced by modern society. The conceptualization of Wisdom Management forms a valuable context to further advance knowledge management theory into practice. It is also a cornerstone of strategic management and can provide insight into its potential for success and implementation.

• 한국수자원학회논문집
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• 제42권12호
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• pp.1103-1111
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• 2009

본 연구에서는 도시화와 기후변화에 대한 미래시나리오를 구성하고 각각의 시나리오별로 수자원 관리 대안들에 대한 효과분석을 수행하였다. 기후변화 시나리오는 SDSM (Statiatical Downscaling Method) 모형을 이용하여 구축하였으며 도시화는 불투수면 모형(Impervious cover model, ICM)을 이용하였다. 안양천 유역에 대해 하수처리수 재이용, 저수지 재개발 대안들을 유황곡선(flow duration curve)과 BOD 농도지속곡선(concentration duration curve)의 변화측면에서 연속유출모형인 HSPF (Hydrological Simulation Program - Fortran) 모형을 이용하여 분석하였다. 그 결과, 시나리오별 하천 유출량에 대한 대안 효과의 차이가 고수량 ($Q_{10},\;Q_5,\;Q_1$)에 비해 저수량($Q_{99},\;Q_{95},\;Q_{90}$)과, 수질오염($C_{30},\;C_{10},\;C_1$) 측면에서 크게 나타남을 알 수 있다. 즉 도시화는 물 순환을 크게 악화시키나 대안의 적용이 이러한 현상을 크게 막아줌을 알 수 있다. 또한 목표 유지유량 및 목표 수질 만족일수는 도시화에 대해 매우 민감한 것으로 나타났다. 따라서 향후 수자원 관리 대안을 수립할 때 기후변화와 도시화에 대한 분석을 포함시키는 것이 바람직하다.

• 자원환경지질
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• 제47권6호
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• pp.673-681
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• 2014

세계 여러 나라들이 대체에너지 개발에 박차를 가하고 있지만 아직까지도 전통적인 탄화수소가 차지하는 비중은 상당히 크며 접근 용이한 지역의 석유자원이 점차 고갈됨에 따라 높은 기술력이 요구되는 한계 유가스전 개발을 위한 탐사수요가 증가하고 있다. 기존 탐사기술인 2차원 탄성파 탐사 및 3차원 탄성파 탐사 기술의 이해는 물론 새로운 기술인 방위각 탄성파 탐사, 해저면 탄성파 탐사, 해양 전자탐사 기술의 개발과 적용을 위한 노력이 필요하다. 이 글은 해저 석유탐사 기술의 개요와 이에 관련된 국내외 연구, 개발, 학술동향 등을 상세히 분석한 것으로, 국내 관련 산업 및 기술정책 수립과 연구개발에 유용한 정책 자료로 활용될 수 있을 것이다. Web of science의 SCI-Expanded DB에 발표된 해저 석유탐사 관련 논문(2001~2014)을 조사한 결과 616건을 대상으로 학술정보를 분석하였다. 발표문헌은 중국이 전체의 22.56%를 점유하여 가장 많은 논문을 발표하였고, 미국과 브라질이 추격하는 양상을 보인다. 국가별 논문 수준 지수 분석 결과, 잉글랜드가 가장 높은 값(1.94)을 보여 논문의 피인용 관점에서 질적 수준이 가장 우수하였고, 미국(1.77), 노르웨이(1.46), 캐나다(1.39), 프랑스(1.39), 스코틀랜드(1.38) 등이 뒤를 이었다.

• 지구물리와물리탐사
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• 제24권1호
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• pp.20-27
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• 2021

암석의 열생산율은 구성 광물 내 방사성 동위원소의 방사성 붕괴와 감마선 방출에 의한 열에너지에 기인하며, 그 크기는 주요 방사성 동위원소인 U, Th, K의 함량에 지배적이다. 그러므로 암반의 열생산율은 암석의 밀도와 주요 동위원소의 함량에 기반해 평가할 수 있다. 심부 암반의 열생산율 평가에 필요한 방사성 동위원소의 농도는 시추공에서 회수된 암추 또는 암편에 대한 질량분석을 통해 파악할 수 있는데, 대심도 시추공의 경우 요구되는 분석량과 그에 따른 시간 및 비용의 문제로 적용이 제한적일 수 밖에 없다. 시추공을 대상으로 하는 물리검층 중 암반의 감마선 스펙트럼으로부터 U, Th, K의 함량을 유추할 수 있는 기술이 있지만, 열생산율 평가를 위해 별개의 밀도검층 자료를 필요로 하며, 상용화된 장비의 적용 심도 또한 아직은 제한적이다. 이에 대한 대안으로 시추공 암반이 방출하는 감마선의 강도를 측정하는 자연 감마선 검층 결과로부터 열생산율을 유추하는 방법이 제안된 바 있으며, 국내외에서 비교적 쉽게 암반의 열생산율을 평가할 수 있는 방법으로 사용되고 있다. 본 기술보고에서는 국내 상용화된 대심도 시추공 물리검층 장비 및 기술을 활용해 감마선 검층 기반의 열생산율 평가 기법을 개발하고, 국내 약 2 km 깊이의 대심도 시추공에 대한 적용 및 검증 사례를 소개하고자 한다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.9-9
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• 2014

Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.