• Resources Recycling
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• 제13권4호
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• pp.32-38
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• 2004

Recently, Orimulsion (a bitumen-in emulsion) has received increasing attention as an alternative fuel. Orimulsion combusion produces an ash rich in V, Ni and Mg which are processed to recover metals. As a basic study to recover V from Orimulsion ash, physico-chemcial properties and leaching behaviours were investigated. Orimulsion ash was fine size grains ($d_{50}$ 5.9 $\mu\textrm{m}$) with 16% V, 4 % Ni and 9% S. Vanadium was easily leached in water because Orimulsion ash was mainly constituted of metal sulfates. However, the increase of leaching temperature decreased the extraction percentage of vanadium because of hydrolysis of V(V) to vanadium pentoxide. The addition of sulfuric acid could increase the leaching percentage vanadium. In case of alkaline leaching for selective recovery of vanadium, the oxidzing agent such as $H_2$$O_2$ is required to improve the leaching per-centage

• Food Science of Animal Resources
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• 제39권4호
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• pp.601-609
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• 2019

Bifidobacterium longum KACC 91563 secretes family 5 extracellular solute-binding protein via extracellular vesicle. In our previous work, it was demonstrated that the protein effectively alleviated food allergy symptoms via mast cell specific apoptosis, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, we cloned the gene encoding extracellular solute-binding protein of the strain into the histidine-tagged pET-28a(+) vector and transformed the resulting plasmid into the Escherichia coli strain BL21 (DE3). The histidine-tagged extracellular solute-binding protein expressed in the transformed cells was purified using Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the process, two liters of transformed culture produced 7.15 mg of the recombinant proteins. This is the first study describing the production of extracellular solute-binding protein of probiotic bacteria. Establishment of large-scale production strategy for the protein will further contribute to the development of functional foods and potential alternative treatments for allergies.

• Food Science of Animal Resources
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• 제43권3호
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• pp.428-440
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• 2023

Global meat consumption is increasing worldwide, however, supply remains lacking. Several alternative protein sources, such as cultured meat, plant-based protein production, and edible insects, have been proposed to overcome this shortage. Interestingly, edible insects are characterized by superior digestive and absorptive qualities that make them the ideal replacement for traditional protein production. This study aims to further the processing ability of insect protein by investigating the effects of various pre-treatment methods, such as blanching (HB), roasting (HR), and superheated steam (HS), on the nutritional properties and physicochemical characteristics of proteins extracted from Hermetia illucens larvae. The drying rate, pH value, color analysis, amino and fatty acid profile, as well as bulk density, shear force, and rehydration ratios of the above pre-treatment methods, were explored. HS was found to have the highest drying rate and pH value analysis showed that HB and HS samples have significantly higher values compared to the other modalities. Raw edible insects had the highest value in the sum of essential amino acid (EAA) and EAA index when compared to EAAs. HB and HS showed significantly lower bulk density results, and HS showed the highest shear force and the highest value in rehydration ratio, regardless of immersion time. Therefore, taking the above results together, it was found that blanching and superheated steam blanching pre-treatment were the most effective methods to improve the processing properties of H. illucens after hot-air drying.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.6-6
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• 2023

Nosemosis is one of the most common protozoan diseases of adult bees (Apis mellifera). Nosemosis is caused by two species of microsporidia; Nosema apis and Nosema ceranae. Nosema ceranae is potentially more dangerous because it has the ability to infect multiple cell types, and it is now the predominant microsporidian species in A. mellifera. In this study, we identified two anti-nosemosis plants, Aster scaber and Artemisia dubia, which reduced the spore development of N. ceranae in spore-infected cells. We intend to establish the anti-nosemosis activity of aqueous, ethyl acetate (EA), and butanol (BuOH) extracts of A. dubia and A. scaber. In order to determine the optimal dose, we did in vitro and in vivo toxicity for all the extracts and carried out anti-nosemosis experiments. Although all of the extracts (aqueous, EA, and BuOH) showed in vitro and in vivo anti-nosemosis activity in a dose-dependent manner, the aqueous extracts of A. dubia and A. scaber showed more potent anti-nosemosis activity than the EA and BuOH extracts. And then, we isolated five phenolic compounds [chlorogenic acid, 3,4-dicaffaeoylquinic acid (3,4-DCQA), 3,5-dicaffaeoylquinic acid (3,5-DCQA), 4,5-dicaffaeoylquinic acid (4,5-DCQA), and coumarin] from A. dubia, A. scaber, and A. dubia + A. scaber aqueous extracts and screened for their toxicities and anti-Nosema effects in both in vivo and in vitro conditions. Among these five compounds, coumarin, chlorogenic acid, and 4,5-DCQA exhibited less toxic but more potent anti-Nosema effects than the other two compounds. Especially, chlorogenic acid and coumarin showed prominent anti-Nosema activities even at the lowest concentration (10 ㎍/mL). They might have potential to be developed as alternative compounds for the control of Nosema disease.

• Food Science of Animal Resources
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• 제44권2호
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• pp.356-371
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• 2024

Novel meat-inspired products, such as cell-cultivated meat and meat analogues, embrace environmental sustainability, food safety and security, animal welfare, and human health, but consumers are still hesitant to accept these products. The appearance of food is often the most persuasive determinant of purchasing decisions for food. Producing cultivated meat and meat analogues with similar characteristics to conventional meat could lead to increased acceptability, marketability, and profitability. Color is one of the sensorial characteristics that can be improved using color-inducing methods and colorants. Synthetic colorants are cheap and stable, but natural pigments are regarded as safer components for novel food production. The complexity of identifying specific colorants to imitate both raw and cooked meat color lies in the differences in ingredients and methods used to produce meat alternatives. Research devoted to improving the sensorial characteristics of meat analogues has noted various color-inducing methods (e.g., ohmic cooking and pasteurization) and additives (e.g., lactoferrin, laccase, xylose, and pectin). Additionally, considerations toward other meat components, such as fat, can aid in mimicking conventional meat appearance. For instance, the use of plant-based fat replacers and scaffolds can produce a marked sensory enhancement without compromising the sustainability of alternative meats. Moving forward, consumer-relevant sensorial characteristics, such as taste and texture, should be prioritized alongside improving the coloration of meat alternatives.

• Journal of Distribution Science
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• 제13권7호
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• pp.63-71
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• 2015

Purpose - The characteristics of the modern management environment in Korea clearly involve the limitations of knowledge management, globalization, changes in industrial structures, polarization of the economy, consumption, and education, and the demand for creative management. To achieve a continuous competitive advantage for individuals as well as organizations, Wisdom Management can be said to be a paradigm for the current environment. The Research is an alternative to environmental management in the early study of the need to conceptualize the theoretical approach to wisdom management. In other words, it presents a new management paradigm for a business environment of uncertainty and unpredictability. Furthermore, it aims to propose Wisdom Management as an alternative in the modern management environment as well as a topic of discourse. Its purpose is to provide basic knowledge to people who study and practice in this field to strengthen the competitiveness of individuals and companies. Research design, data, and methodology - This paper first provides six characteristics of the current management environment at home and abroad. It then attempts to emphasize the necessity of wisdom based on such characteristics by classifying wisdom according to recent management study research. This allows the derivation of definitions of wisdom management, suggesting its components and properties. Results - The six characteristics of the modern business environment are: 1) limits of knowledge, 2) intensifying globalized competition, 3) changes in industry structure - IoT (Internet of all Things) trend, 4) serious polarization and social unrest diffusion, 5) personal and organizational competitiveness creativity requirements, and 6) domestic special national and social situations. Therefore, accordingly, wisdom can be defined and classified as: wisdom that offers potential integration beyond knowledge, practicing and maintaining knowledge wisdom as an efficient strategy for an organization wisdom as a decision-making process that addresses ambivalence wisdom as the pursuit of common goods based on ethics; and, wisdom as creativity that involves intuition and insight. This paper attempts to define Wisdom Management in accordance with these definitions of wisdom. Wisdom Management is a management system that is awareof the current environment given in the multidimensional perspective, achieves the efficiency of the organization and the goals of common good through an uncertain decision-making process, based on the organization of individual and organizational knowledge, and the internal experiences and resources. Wisdom Management components include human beings, virtue, knowledge, creativity, and ambivalent thinking. Its properties include intentionality, self-destructiveness, creative and ethical reflexivity, complexity, insight, unpredictability, ambivalence, and wholeness. Conclusions - Since all organizations face the limitations of their environment, crises, and opportunities, applying wisdom in management to develop as Wisdom Management can be an inevitable alternative to the uncertainties of the management environment faced by modern society. The conceptualization of Wisdom Management forms a valuable context to further advance knowledge management theory into practice. It is also a cornerstone of strategic management and can provide insight into its potential for success and implementation.

• Journal of Korea Water Resources Association
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• 제42권12호
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• pp.1103-1111
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• 2009

This study derived the analysis results of alternatives for integrated watershed management under urbanization and climate change scenarios. Climate change and urbanization scenarios were obtained by using SDSM (Statistical Downscaling Method) model and ICM (Impervious Cover Model), respectively. Alternatives for the Anyangcheon watershed are reuse of wastewater treatment plant effluent, and redevelopment of existing reservoir. Flow and BOD concentration duration curves were derived by using HSPF (Hydrological Simulation Program - Fortran) model. As a result, low flow ($Q_{99},\;Q_{95},\;Q_{90}$) and BOD concentration ($Q_{10},\;Q_5,\;Q_1$) were very sensitive to the alternatives comparing to high flow($C_{30},\;C_{10},\;C_1$). Although urbanization makes the hydrological cycle distorted, effective alternatives can reduce its damage. The numbers of days to satisfy the instreamflow requirements and target water quality were also sensitive to urbanization. This result showed that the climate change and urbanization should be considered in the water resources/watershed and environmental planning.

• Economic and Environmental Geology
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• 제47권6호
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• pp.673-681
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• 2014

Recently, many countries in the world try to develop alternative energy sources, however, traditional hydrocarbon resources are still occupying most of the energy resources. Exploration demands for high technologies are increasing in the development of limited oil & gas resources field owing to the exhaustion of hydrocarbon resources for access area. Therefore, an effort for the development and the application of new technologies such as azimuth seismic survey, ocean-bottom seismic survey and marine controlled-source electromagnetic survey is necessary as well as an understanding of the existing technologies such as 2D/3D seismic survey. This dissertation is designed with the purpose of introducing marine hydrocarbon exploration technologies and analyzing their internalexternal researches, development and science information. In this study, we analised total 616 dissertations for the marine petroleum exploration released in the Sci-expanded DB of 'web of science' during the 2001~2014 periods.

• Geophysics and Geophysical Exploration
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• 제24권1호
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• pp.20-27
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• 2021

Subsurface rock produces heat from the decay of radioactive isotopes in constituent minerals and gamma-ray emissions, of which the magnitude is dominated by the contents of the major radioactive isotopes (e.g., U, Th, and K). The heat production is generally calculated from the rock density and contents of major isotopes, which can be determined by mass spectrometry of drilled core samples or rock fragments. However, such methods are not easily applicable to deep boreholes because core samples recovered from depths of several hundred meters to a few kilometers are rarely available. A geophysical logging technique for boreholes is available where the U, Th, and K contents are measured from the gamma-ray spectrum. However, this technique requires the density to be measured separately, and the measurement depth of the equipment is still limited. As an alternative method, a normal gamma-ray logging tool was adopted to estimate the heat production from the total gamma activity, which is relatively easy to measure. This technical report introduces the development of the proposed method for evaluating the heat production of a granitic rock mass with domestic commercial borehole logging tools, as well as its application to a ~2 km deep borehole for verification.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.9-9
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• 2014

Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.