• 대한약침학회지
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• 제9권3호통권21호
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• pp.61-77
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• 2006

Objectives : Sweet bee venom is made by removing allergen from the bee venom through gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis. The aim of this study was to verify allergy inhibitory action in Sweet Bee Venom in which the allergy causing enzyme is removed. Methods : 95 healthy adult men and women were selected through a survey whom had never received the bee venom therapy in the past. The concentration of bee venom pharmacopuncture and Sweet BV pharmacopuncture was equally at 0.1mg/ml and the experiment was conducted as the double blind test. Experiment groups were classified into low dosage groups(0.1ml for both bee venom pharmacopuncture and Sweet BV) and high dosage groups where 0.4ml of respective administrations were rendered made observations for allergic responses. Results : Participants of the study was comprised of 71 men and 24 women with the average age of 29.0 years. According to results of the low dosage groups, Sweet BV group showed significant reduction in pain after 4 hours and 24 hours compared to the bee venom pharmacopuncture group. Other allergic responses were insignificant between the groups. For the high dosage groups, Sweet bee venom group showed reduction in pain after 30 minutes and 4 hours. Other allergic responses such as edema, itchiness, dizziness from hypersensitivity, and fatigue were significantly lower in the Sweet bee venom administered group after 30 minutes. Conclusions : As a result of removed allergen, Sweet bee venom significantly inhibits allergic responses both locally and throughout the body. This indicates wider and easier application of Sweet bee venom for the symptoms applicable to the bee venom pharmacopuncture. Further comparative studies should be conducted to yield more objective verification.

• 대한의생명과학회지
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• 제27권3호
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• pp.177-181
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• 2021

Allergy is an immune response that appears in certain people, and reactions such as coughing, shortness of breath, and hives occur. The immune system plays an important role in homeostasis and host defense, and allergies cause hypersensitivity reactions when an imbalance of immunity occurs. Mutations in the TLR genes are associated with autoimmune conditions such as allergies and asthma. It has been reported that a locus in the TLR1-TLR6-TLR10 region may be associated with atopic sensitization or allergy. Therefore, the purpose of this study was to select an allergy patient group and a healthy control group to determine how the genetic mutation of TLR1 affects the onset of disease. This study was conducted in 709 patients and 5,025 control groups out of 10,956 patients with data from KARE and HEXA cohorts. As a result of logistic regression analysis of 6 SNPs selected from the TLR1 gene, only rs117033348 showed a statistically significant correlation (P = 0.002356). The influence of rs117033348 was examined using PolyPhen-2, and a significant result was shown. Therefore, it can be predicted that the G base in rs117033348 will have an influence on the human body. In addition, Geography of Genetic Variants browser was used to confirm the geographical distribution of allele frequencies for the TLR1 gene. Although it was found that there was a large racial difference in the prevalence of TLR1 SNP, it could be confirmed that the polymorphism of rs117033348 conducted in this study was only specific in East Asia when compared with each race.

• 대한중풍순환신경학회지
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• 제16권1호
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• pp.19-24
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• 2015

■ Introduction Allergy refers a hypersensitive immune response to the various environmental factors and Qi is considered as vit al energy to defend oneself from environments in Traditional Chinese Medicine. Therefore, this study was performed to assess the relationship between allergy and Qi-deficiency ■ Methods We enrolled the subjects with allergic disorders and the healthy controls without allergic disorders. We got the information on the general characteristics, and the score of Qi-deficiency from all of the subjects, and measured their total Immunoglobulin E(IgE) level. ■ Results There were one hundred forty three subjects with allergic disorders and 211 of the control. The educational level, total IgE, and the scores of the Qi-deficiency were higher in the allergy group than in the control. ■ Conclusion Allergic subjects tend to have more severe Qi-deficiency. This could suggest the clinical effectiveness of some herbal medicines for Qi-deficiency on allergic disorders.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.126-135
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• 2006

In a previous study, a biological response modifier (BRM) specifically enhancing the function of B-cells was isolated from Korean fermented soybean paste (Kfsp), but not from non-fermented soybeans. In this study, we attempted to isolate the bacteria producing the BRM from Kfsp (KfspBRM) by ELISA using anti-KfspBRM and by B-cell proliferation. Five bacteria whose culture supernatants showed the BRM activities were isolated, and one of them was identified as Bacillus licheniformis E1. The bacterial BRM (bBRM) originated from a slime layer of B. licheniformis El had a molecular weight of 1,594 kDa, and contained $33\%\;(w/w)$ of reduced sugar and $4.6\%\;(w/w)$ of protein content. The bBRM appeared to be a glycoprotein that is physically, structurally, and functionally similar to the KfspBRM, suggesting that the isolates including B. licheniformis El may produce the KfspBRM in the fermentation process of soybean paste. The mass production of the BRM by the bacterium may help to study B-cells in immunology, and the enrichment of the BRM in Kfsp may help patients in future who are medically in need of potentiation of B-cell proliferation and antibody production.

• 동의생리병리학회지
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• 제17권2호
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• pp.308-315
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• 2003

This experimental research has been done to study the effects of Sipjeondaebo-tang(SOT) on the anti-allergic reaction. We found the several important results from the research which has been performed by two experiments toward immediately type and delayed type in order to study the effects of SDT on hypersensitivity response to mice. The results obtained from our research are as following. The survival rate of one group to which we injected only the compound 48/80 is almost 0% according to its density and timing test. In the other hand, the survival rates of the other group to which we injected both of the compound 48/80 and SDT are 20%, 10%, 30%, 10%, 40% and 70% according to 0.025, 0.05, 0.1, 0.25 0.5 and 1 (mg/g) of compound 48/80. Time dependency test also shows the 30% and 20% survival rates in 5 and 10 minutes. SDT revealed the significantly inhibitory effect on Compound 48/80 induced Mast cell degranulation. SDT showed the significantly inhibitory effect in the delayed type hypersensitivity response to picry1 chloride. SDT showed the significantly inhibitory effect in the delayed type hypersensitivity response to sheep red blood cell. Our research provides the important evidence that SDT is benificial to the prevention and treatment of allergy related diseases.

• 동의생리병리학회지
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• 제17권4호
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• pp.1002-1007
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• 2003

In order to investigate the effect of Sojagangki-tang on imuno-activity. the author performaed this experimental study. Delayed type hypersensitivity (DTH) and rosette forming cells (RFC) for cell-mediated immune response, hemagglutinin (HA) titers, hemolysin (HL) titers for humoral immune response, Carbon clearance for phagocytic function of MPS (mononuclear phagocyte system) were measured in ICR mice. The results were summurized as follows: Delayed tape hypersensitivity was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Hemagglutinin titers were increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Hemolysin titers were increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Number of RFC was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Carbon clearance was increased with statistical significance in the administrated solid extract of Sojagangki-tang treated group as compared with the control group. Through in vivo experimental study in ICR mice Sojagangki-tang enhences the cell-mediated immmune response, the humoral immune respose. According to the above results, I think that Sojagangki-tang could be used for allergy asthma and lung damage patients.

• Journal of Acupuncture Research
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• 제17권1호
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• pp.169-174
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• 2000

The immune response of Bee Venom Therapy is commonly appear in clinics. It is whole body delayed allergy type and generally like fatigue. Therefore, in order to analysis the clinical form, we have observed immune response of 100 patients who visited Sangji University Oriental Medical Hospital and treated Bee Venom Therapy over 10 times from November 1998 to October 1999. The results were summarized as follows. 1. The distribution of Sex was 60 females, 40 males, and the average of patients age was $50.6{\pm}1.5years$. 2. The distribution of disease was degenerative arthritis, HIVD of L-spine, RA, etc. 3. The total treated time is 2765 and is observed 361 immume responses. 4. The average of keeping time in immume response is $11.8{\pm}0.6(hr)$, and the cases of over 24hrs is occupied 24.0%. 5. In the correlation between treated times and immume response is generally in inverse proportion. 6. The general aspect of immume response is chilling, heating, powerless, headache, dizziness, etc. 7. $M{\ddot{u}}ller$ Grade II-III was observed only 1%.

• 한국식품영양과학회지
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• 제38권2호
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• pp.160-165
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• 2009

본 연구는 황금추출물인 baicalein의 피부알레르기 예방 효과를 알아본 연구로서 baicalein을 투여할 농도에 따라 0 mg/kg(대조군), 50 mg/kg(저용량군), 100 mg/kg(고용량군) 3개의 그룹으로 나누고, ear swelling을 측정하는 1군과 IgE 농도와 histamine 농도의 측정 및 조직병리학적 관찰을 위한 2군으로 나눠 실험을 하였다. Baicalein을 8일에 걸쳐 8회 경구투여를 실시한 후 접촉성 피부알레르기 유발 물질인 PCL을 귀에 감작시켜 알레르기를 유발시키고, 1군에서 ear swelling 변화를 확인하고 2군에서는 1군의 ear swelling의 결과에서 귀의 두께가 최고치를 이루는 시간대에 부검을 실시하여 적출한 조직으로는 HE염색과 TB염색을 실시하여 병변의 유무와 비만세포를 확인하였다. 채취한 혈청으로는 IgE 농도와 histamine 농도를 측정하였다. Ear swelling의 경우 0 mg/kg 농도의 baicalein을 투여한 대조군에 비해 50 mg/kg, 100 mg/kg 농도의 baicalein을 투여한 저용량군과 고용량군 그룹의 귀의 두께 증가폭이 낮게 나타났고, 저용량군보다 고용량군에서 그 증가폭이 낮게 나타났다. IgE 농도와 histamine 농도 측정결과에서도 0 mg/kg 농도의 baicalein을 투여한 대조군에 비해 50 mg/kg, 100 mg/kg 농도의 baicalein을 투여한 저용량군과 고용량군에서 낮은 수치가 나왔다. 조직병리학적 검사결과에서는 육안적으로 모든 그룹의 간과 신장에서는 별다른 병변은 관찰되지 않았고, HE염색을 한 귀 조직에서는 대조군에서 고용량군에 비해 귀의 두께가 두껍게 관찰되었고, TB염색을 한 귀 조직에서는 대조군에 비해 고용량군에서 적은 수의 비만세포들이 관찰되었다. 즉, ear swelling, IgE 농도, histamine 농도 및 조직병리학적 결과를 종합해봤을 때 황금추출물인 baicalein이 접촉성 피부알레르기 예방효과가 있다고 판단되어지고, 향후 baicalein은 항알레르기 치료제로 사용되는 생약제의 주요 성분으로 유용할 것이라 사료된다.

• Clinical and Experimental Pediatrics
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• 제56권9호
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• pp.369-376
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• 2013

A complex interplay between genetic and environmental factors partially contributes to the development of allergic diseases by affecting development during prenatal and early life. To explain the dramatic increase in the prevalence of allergic diseases, the hygiene hypothesis proposed that early exposure to infection prevented allergic diseases. The hygiene hypothesis has changed to the microbial hypothesis, in which exposure to microbes is closely linked to the development of the early immune system and allergic diseases. The intestinal flora may contribute to allergic disease through its substantial effect on mucosal immunity. Based on findings that exposure to microbial flora early in life can change the Th1/Th2 balance, thus favoring a Th1 cell response, probiotics may be beneficial in preventing allergic diseases. However, evidence from clinical and basic research to prove the efficacy of probiotics in preventing allergy is lacking. To date, studies have yielded inconsistent findings on the usefulness of probiotics in allergic diseases. It is difficult to demonstrate an exact effect of probiotics on asthma, allergic rhinitis, and food allergy because of study limitations, such as different first supplementation period, duration, different strains, short follow-up period, and host factors. However, many studies have demonstrated a significant clinical improvement in atopic dermatitis with the use of probiotics. An accurate understanding of the development of human immunity, intestinal barrier function, intestinal microbiota, and systemic immunity is required to comprehend the effects of probiotics on allergic diseases.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.47-55
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• 2007

Objectives : To verify pain relief effects and allergy inhibitory action for the osteoarthritis of the knee joint in Sweet Bee Venom in which allergy causing enzyme is removed. Methods : We randomly allocated 36 participants to treatment group Sweet Bee Venom and Bee Venom. Outcomes on pain reduction were measured by 100mm VAS(Visual Analog Scale). And we recorded into details allergic responses during Pharmacopuncture treatment. Results : Whole body condition and pain rate through VAS measurement were improved significantly in 2 weeks. We could get difference in pain score of two Pharmacopuncture groups significantly in 2 weeks. BV group showed superior reduction in pain compared to the Sweet BV group. But we could not get difference in whole score of two pharmacopuncture groups significantly. On the other hand other allergic responses such as edema, itchiness, pain were significantly lower in the Sweet BV group.