• Archives of Pharmacal Research
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• v.13 no.1
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• pp.101-102
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• 1990

Two alkaloids were isolated from the roots of Glycyrrhiza uralensis Fisch. They were identified as 5, 6, 7, 8-tetrahydro-2, 4-dimethylquinoline (1) and 5, 6, 7, 8-tetrahydro-4-methylquinoline (2) by spectral data. The isolation of alkaloid from glycyrrhiza species is reported for the first time.

• Archives of Pharmacal Research
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• v.10 no.3
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• pp.197-199
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• 1987

Two alkaloids were isolated from an ether-soluble alkaloidal fraction of Panax ginseng. They were identified as 1-carbobutoxy-$\beta$-carboline and 1-carbomethoxy-$\beta$-carboline.

• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.203-211
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• 2000

In this study to produce large-scale scopolamine we were examined in the tumor calli of Datura metel L. induced by Agrobacterium tumefaciens $Ery{101}$. The growth and scopolamine contents of tumor calli were higher under light condition than in dark. The optimum condition of growth and scopolamine production were fluence rate of 16 $\mu$mol $m^{-2}s^{-1}$, spectra of red light region and 16 hour light periods on 50 mL SH liquid medium in 4 weeks culture. To increase of the scopolamine contents in tumor calli, the optimum concentration of nitrogen source were 1.8 mM NH$_4$+ and 40 mM NO$_3$. The optimum elicitor concentration for production of scopolamine were 10 mg/L chitosan and 15 mg/L yeast extract. The effect of precursors were good at the concentration of 0.2 mM tropine and 0.3 mM tropic acid, respectively. In order to increase of growth and scopolamine contents. we induced mutant from Datura metel L. tumor callus. Mutants of tumor calli were obtained by 3 Krad, 4 Krad and 6 Krad of ${60}^Cor-ray$. Among them, 3 Krad tumor callus was excellent on the growth and teratoma induction. The 4 Krad tumor callus was negligible for both growth and teratoma induction. But the 6 Krad tumor callus was the best in growth and teratoma induction. The formation of the mutant calli can be enhanced through hormonal combination of 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L benzyladenine. We carry out selection mutant tumor calli for high content tropane alkaloid and suspension cultures for scopolamine production.