• Journal of Microbiology and Biotechnology
• /
• 제11권6호
• /
• pp.928-933
• /
• 2001

Approximately 0.1 mg/ml of polyvinyl alcohol (PVA) was degraded by the growing cell, Brevibacillus laterospours, for 30 h, and 0.2 mg/ml of PVA was degraded by the cell-free extract that was isolated from Brevibacillus laterosporus. Approximately $0.29{\mu}g$/ml of acetic acid was produced from PVA by using the cell-free extract as a catalyst for 40 min. $V_{max}\;and\;K_m$ value of purified PAV-degradation enzyme was 3.75g/l and 2.75 g/l/min in reaction with EDTA and 3.99 g/l and 2.98 g/l/min in reaction without EDTA, respectively. Molecular weight of the purified enzyme determined by SDS-PAGE was 63,000 Da. Alcohol dehydrogenase and aldehyde dehydrogenase activities were qualitatively detected on a native acrylamide gel by an active staining method, indicating the existence of the metabolic pathway to use PVA as a substrate.

• 한국식품영양과학회지
• /
• 제31권1호
• /
• pp.92-97
• /
• 2002

갈화와 갈근이 알코올성 간손상 흰쥐의 알코올 대사효소계에 미치는 영항을 구명하기 위하여 알코올을 투여한 흰쥐에게 갈화와 갈근 열수추출물을 2수준으로 나누어 5주간 급여한 후 그 효과를 관찰하였다. 체중증가량, 식이섭취량과 식이효율은 알코올 투여시 감소하였으며 갈화와 갈근추출물 급여로 회복되는 경향을 보였다. 장기 무게에 미치는 칡추출물의 영향은 알코올 투여로 증가된 간, 뇌와 심장 무게를 감소시켰는데 갈화가 갈근에 비하여 뇌와 심장 무게의 감소효과가 큰 것으로 나타났다. 알코올 대사효소 활성 변화에서 알코올 투여시 유의적으로 증가된 ADH와 MEOS 활성은 칡추출물 급여시 감소된 반면 AlDH 활성은 대조군에서 유의적으로 감소되었는데 칡추출물 급여시 그 활성이 증가되었다. MAO 활성과 과산화수소 함량도 알코올 투여시 증가되었으며 갈화와 갈근 열수추출물 급여시 유의적으로 감소되었다. 이상의 결과에서 갈화와 갈근 열수추출물 급여는 알코올 중독된 흰쥐의 알코올 대사계 효소 활성 변화에 영향을 미쳐 알코올성 간손상 완화에 효과적일 것으로 사료된다.

• 대한의생명과학회지
• /
• 제22권2호
• /
• pp.53-59
• /
• 2016

Alcoholism is a significant health problem in the world. The liver is the first and primary target organ for alcohol metabolism. Alcohol dehydrogenase and aldehyde dehydrogenase play important roles in the metabolism of alcohol and aldehyde. In this study, I aimed to investigate the eliminatory effects of a Phellinus spp. extract on alcohol metabolism in drunken Sprague-Dawley (SD) rats. Male SD rats were given Phellinus spp. extract at 30 min after 40% (5 g/kg) alcohol ingestion. To assay the effect of Phellinus spp. extract on blood alcohol concentration, blood samples were taken from the tail vein at 1, 3 and 5 h after alcohol ingestion. The concentrations of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase in Phellinus spp. extract treated rat were significantly lower than that of the control with a time-dependent manner. In addition, the alanine aminotransferase and aspartate aminotransferase activities of Phellinus spp. extract-treated groups were altered compared to those of the control group. These results suggest that Phellinus spp. extract intake can have a positive effect on the reduction of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase concentrations in the blood and may alleviate acute alcohol-induced hepatotoxicity by altering alcohol metabolic enzyme activities. Phellinus spp. extract is thus a good nutraceutical candidate.

• 한국식품과학회지
• /
• 제16권1호
• /
• pp.90-94
• /
• 1984

Malo-alcohol 발효효모(醱酵酵母) Schizosaccharomyces japonicus var. japonius St-3에 의한 사과산의 대사경로(代謝經路)를 검토한 결과는 다음과 같다. Schiz, japonicus var. japonicus St-3의 조효소(粗酵素)의 활성(活性)을 측정(測定)한 바, malic enzyme(EC1. 1. 1. 40)의 효소활성(活性)이 malate dehydrogenase(EC1. 1. 1. 37)보다 약 4배 높았으며 양효소(兩酵素)의 기질(基質)인 L-malate에 대한 Km치(値)는 malic enzymed l 3. 125mM, malated dehydrogenase는 4. 761mM로써 기질에 대한 친화성(親和性)에 있어서 malic enzyme 쪽이 훨씬 컸다. malo-alcohol발효(醱酵)과정중 사과산이 소시(消矢)되어 pyruvate가 생성(生成)됨을 확인(確認)할 수있었으며 $Mn^{2+}$에 의해 malic enzyme의 활성(活性)이 촉진(促進)되었다. 이상의 결과(結果)로 미루어 본(本) 공시균(供試菌)에 의한 사과산 대사(代謝)의 주경로(主經路)는 $malate{\rightarrow}pyruvate{\rightarrow}acetaldehyde{\rightarrow}ethanol$의 경로(經路)를 거치는 것으로 추정(推定)할 수 있었다.

• 한국식품위생안전성학회지
• /
• 제10권3호
• /
• pp.163-168
• /
• 1995

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out in the net weight gain, food efficiency ratio, weight of organs, and AST, ALT, lactate dehydrogenase, alkaline phosphatase activities in serum of rats. The results obtained were as follows; Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increased in food efficiency ratio. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not, AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group. The above results suggest that garlic would prevent the metabolic disease of liver by improving hyperlipemia caused by high fat diet.

• 한국식품영양과학회지
• /
• 제26권2호
• /
• pp.319-326
• /
• 1997

Met과 Se의 공급수준이 흰쥐의 알코올대사 효소계에 미치는 영향을 관찰하고자, Sprague-Dawley종의 웅성 흰쥐에게 Se 무첨가 및 정상공급식이 (0, 0.45mg/kg diet)에 Met(0, 3, 9g/kg diet)을 투여한 후 5주와 10주간 사육하여 간조직중의 알코올 대사효소와 항산화 방어 효소계 활성도를 관찰하였다. 알코올은 aldehyde dehydrogenase와 microsomal ethanol oxidizing system 활성도를 유의적으로 증가시켰으며, Met과 Se의 동시 결핍시 현저하게 감소하였고 Met과 Se의 동시 공급군의 경우 알코올 투여기간이 길수록 그 활성이 유의적으로 증가하였다. Aldehyde dehydrogenase 활성도는 알코올 투여로 감소하였으며 Met과 Se 동시 결핍군에서는 현저한 감소를 나타내었고, 사육기간이 길수록 그 활성이 유의적으로 감소되었다. Cytochrome P-450과 xanthine oxidase 활성도는 알코올 투여 시 유의적으로 증가되었는데 Se 결핍시 Met 결핍군과 과량공급군에서 그 증가가 현저하였으며, 5주 실험군에 비해 10주 실험군에서 유의적인 증가를 나타내었다. Superoxide dismutase, catalase 및 glutathione S-transferase 활성도는 알코올 투여시 증가되었으며, superoxide dismutase와 catalase 활성도는 알코올 투여기간이 길수록 유의적인 증가를 나타내었다. 알코올 투여시 glutathione peroxidas 활성도는 감소되었으며, Met 수준이 높을수록 증가하는 경향이었고, Met과 Se 동시 결핍시 현저하게 감소하였다. 이상의 결과에서 식이성 Met과 Se은 동물의 성장 및 정상적 대사유지에 필수적인 영양소로서 알코올 투여 시 Met과 Se 첨가에 따라 알코올대사 효소활성도와 항산화 방어효소 활성도의 변화를 유도할 수 있었다. 또한 식이 kg당 3g의 Met과 0.45mg의 Se 동시 공급시 원활한 알코올대사를 유도하여 알코올성 간손상을 가장 효율적으로 경감시킬 수 있는 것으로 나타났다.

• 대한의생명과학회지
• /
• 제20권3호
• /
• pp.124-128
• /
• 2014

Alcoholism is a significant global health problem. Alcohol dehydrogenase and aldehyde dehydrogenase play important roles in the metabolism of alcohol and aldehyde. In this study, we aimed to investigate the eliminatory effects of a fruit extract drink on alcohol metabolism in drunken Sprague-Dawley (SD) rats. Male SD rats were given a fruit extract drink or a commercial product (10 mL/kg) 30 min prior to 40% (5 g/kg) ethanol ingestion. To assay the effect of the fruit extract drink on blood ethanol concentration, blood samples were taken from the saphenous vein at 3 and 5 h after ethanol ingestion. The blood concentrations of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase were significantly lower in the fruit extract drink group than in the control group, in a time-dependent manner. However, the alanine aminotransferase and aspartate aminotransferase activities of all experimental groups were unaltered compared to those of the control group. These results suggested that fruit extract drink intake can have a positive effect on the reduction of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase concentrations in the blood and may alleviate acute ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme activities.

• 동의생리병리학회지
• /
• 제24권4호
• /
• pp.610-615
• /
• 2010

Alcohol is the most widely psychoactive drug and has known in almost all civilization since ancient time. Recently increase consuming alcoholic beverages, alcohol is on of the major public health problems in the world. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) play important roles in the metabolism of alcohols and aldehydes. The drink consists of medicinal herbs, Puerariae Radix, Phyllostachyos Folium, Citri Pericarpium, Polygonati Rhizoma, Rehmanniae Rhizoma (Vinegar), which have been widely used in oriental medicine. This study was designed to investigate effects of medicinal herbal drink (MHD) on alcohol metabolism in drunken SD rats subjects. In experiment, rats were treated to ethanol (EtOH, 3 g/kg, PO) at 60 min. after saline (CON) or MHD (1 ml/kg, PO) administration. The blood alcohol concentration (BAC), blood acetaldehyde concentration (BALC) activities of ADH, ALDH, AST and ALT were significantly decreased in MHD group than in control group as a time-dependent manner. And drinking water volume in MHD group with duplicate treatment, were significantly decreased than in CON group. These results suggested that MHD intake could give an influence upon the reduction in BAC and BALC may alleviate acute ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme activities.

• 한국식품위생안전성학회지
• /
• 제13권3호
• /
• pp.268-273
• /
• 1998

Lard 와 Alcohol을 섭취시킨 흰쥐 혈청중의 효소 활성에 인삼추출물이 미치는 영향을 검토하기 위해서 흰쥐에 Lard 와 Alcohol 및 인삼추출물을 첨가한 식이를 10주간 급여하여 성장시킨 후 체중, 각종 장기 중량, 혈청중 AST, ALT, ALP, LDH의 활성을 측정하였을 때 다음과 같은 결과를 얻었다. Lard 와 Alcohol 첨가 식이군에서는 체중과 식이섭취량은 감소하였으나, 인삼추출물의 투여에 의한 식이효율은 증가하였다. lard의 첨가 식이로 성장시킨 실험군에서는 간장, 신장, 비장의 무게는 증가하였으나 다른 식이 군에서의 중량변화는 없었다. 혈청중 AST, ALT, LDH, ALP 활성은 lard , alcohol을 첨가한 식이군(II, III군)에 비해 유의한 감소를 나타내었다. 이상의 실험 결과로 보아 Lard 와 Alcohol의 만성적인 섭취는 실험동물의 간기능에 손상을 유발하여 혈청 중 AST, ALT, ALP, LDH활성이 증가하였으나 인삼추출물을 첨가한 실험군에서는 위와 같은 효소의 활성이 유의적으로 감소하였다. 따라서 인삼추출물은 고지방식이로 인한 고지혈증을 개선함으로써 간조직의 손상을 예방하여 줄것으로 생각되어 진다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제3권1호
• /
• pp.15-18
• /
• 1998

The entanol productivity of superoxide dismutase (SOD)-deficient mutants of Saccharo-Myces cerevisiae was examined under the oxidative stress by Paraquat. It was observed that MnSOD-deficient mutant of S. cerevisiae had higher ethanol productivity than wild type or CuZnSOD-deficient yeast both in aerobic and in anaerobic culture condition. Pyruvated dehydrogenase activity decreased by 35% and alcohol dehydrogenase activity increased by 32% were observed in MnSOD-deficient yeast grown aerobically. When generating oxygen radicals by Paraquat, the ehanol productivity was increased by 40% in CuZnSOD-deficient or wild strain, resulting from increased activity of alcohol dehydrogenase and decreased a activity of pyruvate dehydrogenase. However, the addition of ascorbic acid with Paraquat returned the enzyme activities at the level of control. These results imply that SOD-deficiency in yeast strains may cause the metabolic flux to shift into anaerobic ethanol fermentation in order to avoid their oxidative damages by Paraquat.