• 제목/요약/키워드: alcohol fermentation

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Production of Fermented Honey Wine (벌꿀발효주의 제조)

  • Rhim, Jong-Whan;Kim, Dong-Han;Jung, Soon-Teck
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.337-342
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    • 1997
  • In order to compare methods of making a mead and a melomel, changes of alcohol contents, reducing sugar, soluble solids, pH and total acidity during fermentation of a mead and Japanese plum melomel was investigated. Fermentation rate of the melomel were much faster than the mead. Reducing sugar and soluble solids were continuously decreased until the 16th day of fermentation, while alcohol contents were increased continuously during the same period. After fermentation of 21 days, alcohol contents of the mead was reached 7.6%, while that of the Japanese plum melomel reached 12.4%. pH and total acidity were not changed considerably during the whole fermentation period. Clear honey wines with transmittance of 99.4% were obtained by membrane filtration. In conclusion, the method of making melomel using the osmotically extracted fruit juice with honey was found to be more advantageous than the method of making a simple mead.

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Alcohol Fermentation of Korean Watermelon Juice (수박쥬스의 알콜발효에 관한 연구)

  • Kim, Seong-Lim;Kim, Woo-Jung;Lee, Sang-Yeol;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.27 no.3
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    • pp.139-145
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    • 1984
  • For the ultimate manufacture of vinegar with watermelon juice, we examined the preliminarily alcoholic fermentation of watermelon juice using Saccharomyces cerevisiae, Saccharomyces cerevisiae var. ellipsoideus and Kluyveromyces fragilis. The juice contained 3.2% total reducing sugar by the Somogyi-Nelson method. Strains of yeast culture, pH, temperature and concentration of sugar in the juice were important factors affecting alcoholic fermentation. Saccharomyces cerevisiae var. ellipsoideus produced 5.3% alcohol from the juice fortified with 12% glucose under the conditions of pH 5.73 and $27^{\circ}C$ for 8 days. Generally, small quantities of various salts and N-sources affected little the alcoholic fermentation of the juice. The sterilization of the juice by autoclaving improved efficiency of fermentation than that by $SO_2$ sterilization. Determination of alcohol was carried out by the gas chromatographic method using Chromosorb W: Carbowax as packing material.

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Screening of a Potent, Raw Naked Barley Saccharifying Enzyme Producer and Its Application on the Uncooked Alcohol Fermentation (쌀보리 전분 당화효소 생산균의 분리 동정 및 무증자 알코올 발효에의 이용)

  • Oh, Sung-Hoon;Kwon, Ho-Joeng;O, Pyong-Su
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.408-413
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    • 1987
  • Microorganisms capable of degrading the raw naked barley were isolated from soil, and the amylase productivity of each strain was examined on plate contained 2% raw naked barley. Of the fungi and actinomycetes tested, 71 strains were subjected to subsequent testing for amylase production, and 4 strains were selected as potent amylase producers. Among them, Strain No. 281 produced the most potent raw naked barley saccharifying enzyme, and was identified as genus Rhizopus from morphological and physiological studies. The ratio of raw starch saccharifying activity (RDA) of the crude enzyme derived from the Rhizopus sp. No. 281 was showed 2-3 fold higher than that of commercial enzyme when the raw naked barley was used as the substrate. In the case of uncooked alcohol fermentation using Rhizopus sp. No. 281 glucoamylase preparation, the alcohol yield of the broth was 2% higher than that of the commercial enzyme.

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Comparison of Fermentation Characteristics of Korean Traditional Alcoholic Beverages Prepared by Different Brewing Methods and Their Quality Changes after Aging (전통주의 제조방법별 발효 특성 및 숙성후 품질변화 비교)

  • Kim, In-Ho;Park, Wan-Soo;Koo, Young-Jo
    • Journal of the Korean Society of Food Culture
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    • v.11 no.4
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    • pp.497-506
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    • 1996
  • Fermentation characteristics and quality changes of the typical Korean traditional alcoholic beverages (Kwahaju, Sokokju, Baikhaju, Samhaeju and Hosanchun) were investigated during fermentation and after aging, respectively. They were prepared by their own brewing methods with respect to the same contents of rice and Nuruk (Korean-style bran koji). The consumed total sugar content of the alcoholic beverages (Sokokju, Baikhaju, Samhaeju and Hosanchun) which were brewed with the multiple input steps of seed mash and raw material (ABMIS) was higher than that of the alcoholic beverage (Kwahaju) which was brewed with the single input step (ABSIS). The pH values of all the beverages were similar and were ranged from 3.2 to 3.6. The acidity of ABMIS were lower than that of ABSIS, but alcohol concentration of ABMIS were higher. The fermentation period of the alcoholic beverages with triple input steps was the longest among them. The inner temperature and microbial growth in all the beverages increased at each of input steps of the raw materials. Fusel oil content of ABMIS was lower than that of ABSIS. Major components of the fusel oil were iso-amyl alcohol, iso-butanol, n-propanol, ethyl acetate and acetaldehyde, and its minor components were methanol and n-butanol. The results on quality changes of the alcoholic beverages after aging showed increase of alcohol in ABMIS, but decrease of sugar and acidity. Fusel oil contents of Kwahaju and Samhaeju decreased to be ranged from 3.1% to 13.9% after aging, but those of Sokokju, Baikhaju and Hosanchun increased to be ranged from 10.9% to 23.0%. Sensory evaluation of ABMIS showed better scores than that of ABSIS. It was suggested that the brewing method with multiple input steps of seed mash and raw material was one of the methods to increase fermentation efficiency for brewing the Korean traditional alcoholic beverages.

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Fermentation Characteristics of Ginpi Wine with Different Levels of Added Ginpi (진피첨가량을 달리한 진피주의 발효특성)

  • Kim, Na-Young;Yu, A-Reum;Min, Jin-Young;Han, Myung-Joo
    • Journal of the Korean Society of Food Culture
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    • v.26 no.2
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    • pp.178-183
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    • 2011
  • The objective of this study was to determine the quality and sensory characteristics of ginpi wine using different levels of ginpi at each fermentation step. In the first mash with or without ginpi, lactic acid bacteria and yeast counts were maximum between days 2 and 3 of fermentation. The reducing sugar content increased after 2 days of fermentation of the first mash. Brix content increased rapidly after 1 day of fermentation, but pH decreased rapidly. After 3 days of fermentation of the second mash, 0, 30, 60, or 90 g of ginpi were added, and the yeast counts were 8.89, 8.72, 8.81, and 8.88 \log CFU/mL, respectively but then continually decreased. After 3 days of ginpi wine fermentation of the second mash, the addition of 0, 30, 60, 90 g ginpi resulted in alcohol content of 11.40, 8.90, 9.40, and 8.95%, respectively, and after 3 days of fermentation, alcohol content increased slightly. The results of a sensory evaluation showed that overall acceptability of ginpi wine was not different with different levels of added ginpi. However, the flavor of the 90 g ginpi wine had the highest acceptability.

A Study on the Apple Fermentation According to Product Various Salt (소금의 종류에 따른 사과발효산물의 특성 연구)

  • Lee, Eun-Suk;Park, Jong-Min;Kim, Jong-Soon;Lee, Se-Yong;Choi, Won-Sik
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.4_2
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    • pp.595-602
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    • 2022
  • This study was conducted to investigate the characteristics of apple fermentation when different types of salt were used during apple fermentation, and when salt was added or not. The pH decreased in all experimental groups during the fermentation process, and the final pH was 4.72-3.31. The sugar content was 17.8-18.5 °Brix, the lowest in the sea salt (FAS), and the highest in the control group (FA). The acidity was measured as 0.48-0.56%, which was high in the control group (FA), and was 0.83-1.06% on the 7th day of final fermentation. The alcohol content was generated from the 5th day of fermentation, and the control group (FA) showed a distribution of 39.23-29.4%, and the alcohol content was higher in the control group than in the experimental group, As for the total polyphenol content and total DPPH content, it was confirmed that the purified salt (3.11 mg GAE/mL) showed the highest total polyphenol content than the control (2.25 mg GAE/mL). When salt was added, the fermentation progress was excellent, and when the salt concentration was high, it was confirmed that the possibility of apple fermentation was high.

Study of Continuous Production of Alcohol using Biologically Sandwich-styled Immobilization Carrier (샌드위치식 고분자담체를 이용한 알코올 연속생산연구)

  • Park, Young-G.;Kim, Hee-Jung
    • KSBB Journal
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    • v.23 no.3
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    • pp.213-218
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    • 2008
  • The present study was to investigate the continuous production of alcohol using immobilized carrier manufactured by polymeric materials. Fermentation runs with a crushed rice, with constituents recovered from batch culture and with ones from continuous culture were thus compared. The performances of immobilized carrier were governed by sandwitched synthetic polymers, the evolution of the continuous culture was steadily governed by the production of alcohol in the lag time of batch culture. The main focus was set on the enhancement of the alcohol production by an newly-developed polymeric forms. This polymeric form led to a drastic increase of the microorganism and the production cost in the continuous reactor was thereby reduced. The sandwitched polymeric-formed carrier, which was resistant to external environments, serves as an interesting alternative to maintain the stability of biological process. These whole results were discussed with the aim to better understand the continuous process implied in the microorganism's build-up during cultivation of fermentation broth.

Manufacturing of Korean Traditional Rice Wine by using Gardenia jasminoides (발효법을 달리하여 제조한 치자꽃 발효주의 특성)

  • Cho, Soo-Muk;Kim, Jae-Ho;Park, Hong-Ju;Chun, Hye-Kyung
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.413-415
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    • 2009
  • To develop a new traditional rice wine by using Gardenia jasminoides, various fermentation methods such as without cooking, with cooking and with starter seed methods were studied. The condition of alcohol fermentation was investigated by addition of 1% Gardenia jasminoides into mash. Among the fermented methods, the fermentation with starter seed was the best as the alcohol was 19%. The acceptability of the Gardenia jasminoides rice wine with different methods were compared. The starter seed method which was prepared by adding 1% Gardenia jasminoides into mash showed the best acceptability in the sensory evaluation test and color test.

The Eueet of Ginseng Extract on Physiology of Saccharomuces cereuisiae (인삼추출물이 Saccharomyces cerevisiae의 생리에 미치는 영향)

  • 주현규;이교철
    • Journal of Ginseng Research
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    • v.3 no.2
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    • pp.95-104
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    • 1979
  • The effects of ginseng extracts on carbon dioxide generation, alcohol fermentation, and yeast cell production by Saccharomyces cerevisiae were investigated. The results are as follows. 1) In the process of fermentation, CO2 generation by yeast is faster in ginseng extracts media of 0.3%, 0. l% than in control. As the concentration of the extracts increases by 0.7% and 1.5%, CO2 generation is decreased. Among all these concentrations, CO2 generation is fastest in 0.3% of the extracts. 2) In the process of fermentation, the production of alcohol is larger in the order of 0.3%, 0.7% and 0.1% than in the control and least in 1.5%. 3) The number of yeast cell rapidly increased from 12 hours to 18 hours after cultivation and conspicuously increased in the order of 0.3%, 0.7%, 0.1%, control and 1.5%. 4) Dried yeast cell weight increased more in all the above concentration than control and among these it increased visibly in 0.3% of the extracts.

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Production of Alcohol from Starch without cooking: A chemical gelatinization method (무증자(無蒸煮)전분법에 의한 알코올생산(生産): 화학적(化學的) 호화법(糊化法))

  • Park, Kwan-Hwa;Oh, Byung-Ha;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.27 no.1
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    • pp.52-54
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    • 1984
  • Ethanol fermentation from the chemically gelatinized starchy material was examined. The critical concentration of sodium hydroxide solution for gelatinization was dependent on the species of starch; 0.4M for potato and 0.6M for tapioca at room temperature. For alcohol fermention the starchy material was gelatinized by addition of sodium hydroxide solution, neutralized by sulfuric acid, and then yeast was added. The amount of $CO_2$ evolved during ethanol fermentation indicates that non-fermentable material was not produced from the starch by chemical gelatinization. In ethanol fermentation of potato and tapioca starch no significant difference was observed between the thermal and the chemical gelatinization.

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