• 한국식품위생안전성학회지
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• 제26권4호
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• pp.370-376
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• 2011

본 연구에서는 한방에서 약용으로 사용하고 있는 미나리과에 속하는 천궁을 에탄올 추출을 사용하여 고지방식 이에 의해 유발된 고지혈 및 비만 흰쥐에서의 항산화 활성과 간 보호 효과에 미치는 영향을 관찰하였다. 고지방 식이에 의해 유도된 고지혈 및 비만 흰쥐에 있어서의 간 손상에 대한 천궁 추출물의 보호효과를 연구한 결과 고지방 식이를 먹인 흰쥐의 혈청 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT)는 증가 되었고, 간조직의 균질액에서 과산화지질의 최종 산물인 MDA도 높게 나타났다. 이와 반대로 천궁 추출물을 처치한 군에서는 간 기능 지표효소의 증가를 억제시켰다. 간조직의 항산화 효소인 SOD, catalase와 GPx의 활성은 고지방식이 의해 감소되었고, 천궁 추출물의 투여로 인해 효소 활성도 가증가됨을 볼 수 있었다. 본 연구를 통해 천연물을 이용한 기반연구로서 향후 천궁의 지질개선 효과와 항산화 효과에 대한 분자생물학적 조직병리학적 후속연구가 필요하다고 사료되며 이 연구의 결과를 바탕으로 천궁의 항산화 식품이나 지질개선 기능성 식품소재로의 활용이 기대되어진다.

• 대한본초학회지
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• 제21권3호
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• pp.47-54
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• 2006

Objectives : This study was performed to compare the preventive effect of water extracts from Injinho-tang and Haninjinho-tang on hyperlipidemia and liver damage induced by alcohol. Methods : Except for the normal group, we fed rat on 25% alcohol for 55 days. And each extract was administrated for the same period. We measured the serum component in rat's blood, body weight and weight of liver. Results : 1. At first, we observed effects of Injinho-tang and Haninjinho-tang on hyperlipidemia induced by alcohol. Injinho-tang group showed significant decrease of triglyceride, body weight in comparison with those of the control group. Haninjinho-tang group showed significant decrease of total cholesterol, triglyceride in comparison with those of the control group. I 2. At second, we observed effects of Injinho-tang and Haninjinho-tang on liver damage induced by alcohol. lnjinho-tang group showed significant decrease of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), liver weight in comparison with those of the control group. Haninjinho-tang group showed significant decrease of AST (aspartate aminotransferase), ALT (alanine aminotransferase) in comparison with those of the control group. Conclusion : Reviewing these experimental results, it appears that water extracts from Injinho-tang and Haninjinho-tang have pharmaceutical preventive efficacy on hyperlipidemia and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

• 생물정신의학
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• 제5권2호
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• pp.235-242
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• 1998

Objectives : Alcohol-induced oxidative stress has been known to injure various tissues or organs. This stress is related with free radicals which are produced as the result of long-term alcohol consumption. Malonyldialdehyde(MDA) is produced by the interaction of free radicals and cell membrane lipids, and indicates the degree of lipid peroxidation indirectly. The purpose of this study was to investigate the relationship between red blood cell(RBC) membrane lipid peroxidation by free radicals, and associated hepatic injuries and hematologic changes. Methods : Thirty-three subjects diagnosed as alcohol dependence according to DSM-IV diagnostic criteria were evaluated within 72 hours after discontinuing alcohol drinking. Clinical characteristics were evaluated by CAGE questionnaire and Korean Michigan Alcoholism Screening Test(MAST). RBC membrane MDA level was measured as the marker of RBC membrane lipid peroxidation. Aspartate aminotransferase(AST), alanine aminotransferase(ALT) and gamma-glutamyltransferase(GGT) were used as the biochemical markers of liver damage due to alcohol ingestion. The alcohol-induced hematologic change was assessed by mean corpuscular volume(MCV). Results : The results were as follows. Clinical characteristics were not different between two groups having normal and abnormal levels of AST, ALT, GGT or MCV. The levels of MDA were not correlated with the clinical characteristics and serum levels of AST, ALT and GGT. However, there was a significant correlation between the levels of MDA and the value of MCV(p=0.017). Conclusions : These findings suggest that oxidative stress in alcohol dependence may not be reflected in liver enzyme markers such as AST, ALT and GGT, but may be reflected in MCV.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.51-54
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• 2000

1. E.coli에서 별도의 expression vector를 사용하지 않고도 Bacillus 균주 유래의 유용효소(D-Amino acid aminotransferase;D-AAT, Aspartate aminotransferase;AspAT, Alanine dehydrogenase;AlaDH 등)의 유전자의 5'-up stream부위의 발현 기구를 검토한 결과 각 유용 효소의 유전자들의 5'-upstream부위에 존재하는 프로모터들이 연속적으로 존재한다는 사실이 유추되었고, E.coli의 SD sequence와 매우 상동성이 높은 서열 또한 존재함을 확인하였다. 2. 유용 효소 유전자의 번역과 관련된 5'-upstream 부위의 분석을 통하여 유용 효소의 유전자들은 E.coli의 ribosomal RNA와 매우 안정한 SD pairing을 형성(D-AAT의 경우: -13.0kcal/mol, AspAT의 경우; -9.5kcal/mol, AlaDH의 경우 -15.8kca1/mol)할 수 있음을 확인하여, 이러한 높은 자유에너지 변화는 E.coli내에서 유용 효소의 번역에 기여함을 예상할 수 있다.

• 대한한의학회지
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• 제31권2호
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• pp.149-157
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• 2010

Objective: The purpose of this study was to investigate the level of safety on liver functions when Korean herbal medicine was taken internally. Method: 101 inpatients who took Korean herbal medicine were enrolled and liver function test (aspartic aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, gamma glutamyltranspeptidase, albumin, lactate dehydrogenase) was performed on admission and 1 month later. Results: In 101 patients, alkaline phosphatase and gamma glutamyltranspeptidase decreased significantly compared with the value taken on admission (p<0.05) but aspartic aminotransferase, alanine aminotransferase, total bilirubin, albumin, and lactate dehydrogenase were not significantly changed (p>0.05). In the patients who took Scutellaria baicalensis (n=34), alkaline phosphatase decreased and albumin increased significantly (p<0.05). Among the patients who took Atractylodes macrocephala (n=29), alkaline phosphatase decreased significantly (p<0.05). In the patients who took Glycyrrhiza uralensis or Paeonia lactiflora, liver function parameters were not significantly changed (p>0.05). On admission 11 patients had abnormal liver function and 2 patients had liver injury while 7 patients had abnormal liver function and 2 patients showed liver injury 1 month later. Conclusions: This study suggests that prescribed Korean herbal medicine does not injure liver function.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.188-205
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• 2001

Objective : The purpose of this studies was to determine the effect of Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. on changes of trabecular area and physiological metabolites in the ovariectomized osteoporotic Rats. Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then the Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. were orally administerd: Such indexes were measured as the changes of body weight, bone mineral density, trabecular area in tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus, cholesterol, triglyceride, aspartate aminotransferase and alanine aminotransferase in serum. Results : 1. The change of bone mineral density in Bogol-Tang group and Rhynchosia volubilis lour. group. was significantly increased compare to control group. 2. The change of trabecular area % in epiphysis of tibia in Carthami semen group was significantly increased compare to control group. 3. The change of trabecular area % in diaphysis of tibia in Bogol-Tang group was significantly increased compare to control group. 4. The change of serum osteocalcin in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 6. The change of1 serum calcium in Rhynchosia volubilis lour. group was significantly decreased compare to control group. 7. The change of phosphorus in Carthami semen group and Rhynchosia volubilis Lour. group was significantly decreased compare to control group. 8. The change of serum cholesterol and triglyceride of experimental groups were decreased in comparison with control group. 9. The change of serum AST(Aspartate aminotransferase : GOT) in Rhynchosia volubilis Lour. group was decreased compare to control group. 10. The change of serum ALT(Alanine aminotransferase : GPT) in Bogol-Tang group was decreased compare to control group.

• 미생물학회지
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• 제45권4호
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• pp.416-418
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• 2009

번데기동충하초의 간독성 회복 효과를 HaM/ICR 계열의 흰쥐를 이용하여 검정하였다. 벤조피렌 복강주사시, 혈중의 aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT)의 효소활성과 간조직의 lipid peroxide (LPO) 농도는 대조군과 비교시 급격히 증가하여 간독성을 유발하였다. 그러나, 벤조피렌 대조군과 비교시, 번데기 동충하초 배양액의 경구 투여시 AST와 ALT의 효소활성 및 LPO 농도는 감소하였으며, 벤조피렌에 유도된 간 조직의 glutathione 농도 및 glutathione S-transferase 효소활성은 증가하여 간독성을 회복하였다. 실험결과는 번데기 동충하초 균사체 배양액이 벤조피렌 유발 간독성에 대해 회복 효과가 있음을 보여준다.

• 수산해양교육연구
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• 제24권4호
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• pp.591-601
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• 2012

양식 넙치에 Edwardsiella tarda 또는 Streptococcus iniae를 단독 또는 혼합감염하여 넙치에서 병원성을 비교하였다. 넙치에 단독 또는 혼합감염 후 혈액생화학적 성상으로 alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose 농도, total protein 농도를 조사하였으며, 라이소자임 활성, 신장과 비장 조직 중의 세균수, 조직병리학적 변화와 누적폐사율을 조사하였다. 혼합감염구는 다른 시험구에 비하여 누적폐사율, 신장과 비장 조직 중의 세균수, AST활성과 병리조직학적 변화가 크게 나타났으나, 라이소자임 활성에서는 유의적인 차이가 보이지 않았다. 따라서 E. tarda와 S. iniae의 혼합감염은 신장과 비장 조직 중의 세균수 증가와 내부 장기의 손상으로 숙주에 높은 병원성을 나타낸 것으로 판단된다.

• 동의생리병리학회지
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• 제32권6호
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• pp.418-421
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• 2018

The purpose of this study was to evaluate the changes of liver function test before and after treatment in patients admitted to Korean medicine hospital. We checked liver function test level (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, direct bilirubin) of 147 patient who admitted in Korean medicine hospital from July 1, 2015 to June 30, 2018. The subjects were selected those who took herbal medicine continuously during the admission period and who performed liver function test on admission and before discharge. And the subjects were excluded those who had a history of liver and biliary disease at the time of admission or who took hepatoprotectants. Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase significantly decreased compared with the values of admission(p<0.05), but total bilirubin and direct bilirubin were not significantly changed(p>0.05). On admission 31 patients(21.1%) had abnormal liver function and 6 patients(4.1%) had liver injury while 19 patients(12.9%) had abnormal liver function and 6 patients(4.1%) showed liver injury before discharge. This study suggests that herbal medicine may not injure liver function.

• 한방비만학회지
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• 제18권2호
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• pp.74-82
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• 2018

Objectives: The objective of this study is to investigate the effects of Silbi-um (SBU) extract on the alcoholic fatty liver induced by EtOH administration for 8 weeks. Methods: Male Sprague Dawley rats were used. All animals were randomly divided into 3 groups; Normal, EtOH and EtOH+SBU. The rats of EtOH group were daily treated with ethanol of 25% (v/v) for 8 weeks (n=10). EtOH+SBU group was orally treated with SBU water extract after ethanol administration (n=10). The rats of Normal group were treated with saline (n=10). After 8 weeks, the mean body weight, liver weight, and liver-body weight ratio were calculated. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of all groups were measured. The morphological alterations were observed using hematoxylin and eosin (H&E) and Oil Red O staining. Moreover, the alteration of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) levels were analyzed immunohistochemistrically. Results: The histological data showed that liver sections from EtOH group displayed severe steatosis. SBU extract significantly inhibited the progression of the alcoholic liver injury. The increased serum level of ALT and AST induced by ethanol administration were decreased by SBU extract. Furthermore, SBU extract significantly decreased the liver concentrations of $TNF-{\alpha}$. Conclusions: SBU water extract attenuated the alcohol induced fatty liver by improving hepatic lipid metabolism via suppression of $TNF-{\alpha}$ protein. SBU could be effective in protecting the liver from alcoholic fatty liver.