• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1081-1087
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• 2011

An internally radiating photobioreactor was applied for the production of astaxanthin using the unicellular green alga Haematococcus pluvialis. The cellular morphology of H. pluvialis was significantly affected by the intensity of irradiance of the photobioreactor. Small green cells were widespread under lower light intensity, whereas big reddish cells were predominant under high light intensity. For these reasons, growth reflected by cell number or dry weight varied markedly with light conditions. Even under internal illumination of the photobioreactor, light penetration was significantly decreased as algal cells grew. Therefore, we employed a multistage process by gradually increasing the internal illuminations for astaxanthin production. Our results revealed that a multistage process might be essential to the successful operation of a photobioreactor for astaxnthin production using H. pluvialis.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.2
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• pp.140-153
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• 2011

The objective of this paper is to investigate into the design of photbioreactor for mass production of microalgae. Characteristics of previously developed photobioreactors were examined to obtain design factors, including light transfer, fluid dynamics and metabolic reactions, of the photobioreactor. Design technology of components related to the design factors, such as light sources, photobioreactor cases, spargers, mixer, etc., was discussed to improve the viability and the growth rate of microalgal and productivity of the photobioreactor. Finally a principle direction of the design for an airlift flat plane photobiorectors was investigated.

• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.304-308
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• 1994

A suitable culture method and bioreactor type for itaconic acid production were chosen by comparing the maximal concentration of itaconic acid produced in various systems. In batch culture, the maximal concentration of itaconic acid produced in a bubble column reactor was about 5% greater than that produced in stirred-tank or external-loop airlift reactor. These results were thought to be due to lower shear force and higher mass transfer efficiency in a bubble column reactor in comparison with other reactors. Moreover, the fed-batch mode in a bubble column was found to be a suitable one, producing about 25% higher concentration of itaconic acid compared to batch mode.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.343-345
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• 2002

This study was performed by the airlift bioreactor using the nitrifier consortium entrapped in polyvinyl alcohol(PVA) for removing low concentration total ammonia nitrogen(TAN). At the aeration rate of 1.5 vvm, TAN removal rate and removal efficiency was 316.6${\pm}$7.2 $g/m^3$ day and 92.8${\pm}$2.2%. Removal rate was continuously increased with decreasing from 0.5hr to 0.05hr of hydraulic residence time(HRT), whereas removal efficiency was decreased with decreasing HRT.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.346-348
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• 2002

This study estimated the effect of influent TAN concentration. temperature and pH in the airlift bioreactor(aeration rate; 1.5 vvm, HRT 0.35hr) using immobilized nitrifiers by PVA. At the effect of influent TAN concentration, removal rate was increased with increasing it and removal efficiency maintained 93${\pm}$2%. The optimum temperature for nitrification was $30^{\circ}C$ and at this point. removal efficiency was 95.5${\pm}$1.5%. It was effective to nitrify at $10^{\circ}C$ of low temperature. In the pH range from 7 to 9 in the bioreactor. removal rate and removal efficiency was 310${\pm}$10 $g/m^3$ day and 94${\pm}$3%.

• Korean Management Science Review
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• v.33 no.1
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• pp.89-99
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• 2016

A scheduled airlift is the most critical part of the air transportation operations in ROK Air Force. The military cargo-plane routing problem is a PDPLS (pickup and delivery problem in which load splits and node revisits are allowed). The cargo which is transported by a military cargo-plane is measured in pallet. The efficiency of pallet needs to be considered with respect to its volume and weight. There are some guidelines about orders of priorities and regulations in the military air transportation. However, there are no methodologies which can raise the efficiency of flight scheduling using Operations Research (OR) theories. This research proposes the effective computing methodology and the related heuristic algorithms that can maximize the effectiveness of the path routing model.

• Journal of Environmental Science International
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• v.13 no.10
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• pp.929-938
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• 2004

Sludge discharger applied the principle of the air lift pump was investigated experimentally for the different design( diameter of discharge pipe, diameter and height of the inside and outside wall) and operating parameters(air flow rate, water level). And it was conducted that performance comparison about sludge discharger and conventional air lift pump. The result indicated that discharged liquid were increased with the increase of air flow rate and water level and decrease distance between inside and outside wall. The discharge pressure was increased with an increase of air flow rate and a decrease of the diameter of the discharge pipe, for both the sludge discharger and the airlift pump. The discharge pressures of the sludge discharger were 3-6 times higher than those of the air lift pump.

• Applied Chemistry for Engineering
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• v.8 no.5
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• pp.816-821
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• 1997

The hydrodynamics and the liquid flow characteristics were investigated in an internal circulation airlift reactor with a single nozzle as a gas distributor. In an air-water system, the gas holdup in the individual flow zone and the impulse-response curve of tracer were measured at various gas velocities and reactor heights. Experimental results showed that for the higher gas velocity(>about 8 cm/s), the flow behavior of bubbles in the riser was turbulent flow due to strong bubble coalescences and the axial height of dispersion zone of large bubbles having uniform sizes in the downcomer was decreased with increasing gas velocity. And mean gas holdups in the individual flow zone and the reactor were increased with increasing gas velocities and were decreased with increasing heights of the top section of the reactor and it was decreased with increasing the height of the top section and gas velocity. Flow characteristics of liquid in the riser and the downcomer was tend to access to plug flow and the overall flow behavior of liquid was mainly varied with the size of the top section which it was assumed to be perfect mixing zone. In these conditions, liquid circulation velocities were increased with increasing gas velocities and they were higher than those by using other gas distributors.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.359-371
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• 2019

To optimize the expression and secretion of ferritin protein associated with ion storage in the mushroom, Pleurotus eryngii, a recombinant secretion vector, harboring the ferritin gene, was constructed using a pPEVPR1b vector under the control of the CaMV 35S promoter and signal sequence of pathogen related protein (PR1b). The ferritin gene was isolated from the T-Fer vector following digestion with EcoRI and HindIII. The gene was then introduced into the pPEVPR1b secretion vector, and it was then named pPEVPR1b-Fer. The recombinant vector was transferred into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformants were selected on MCM medium supplemented with kanamycin and its expression was confirmed by SDS-PAGE and western blotting. Expression of ferritin protein was optimized by modifying the culture conditions such as incubation time and temperature in batch and 20 L airlift type fermenter. The optimal conditions for ferritin production were achieved at 25℃ and after incubating for 8 days on MCM medium. The amount of ferritin protein was 2.4 mg/g mycelia, as measured by a quantitative protein assay. However, the signal sequence of PR1b (32 amino acids) seems to be correctly processed by peptidase and ferritin protein may be targeted in the apoplast region of mycelia, and it might not be secreted in the culture medium. The iron binding activity was confirmed by Perls' staining in a 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in P. eryngii mycelia. Mycelium powder containing ferritin was tested as a feed additive in broilers. The addition of ferritin powder stimulated the growth of young broilers and improved their feed efficiency and production index.

• Korean Chemical Engineering Research
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• v.40 no.6
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• pp.763-768
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• 2002

This study was performed in the airlift bioreactor using the nitrifier consortium entrapped in polyvinyl alcohol(PVA) for removing low concentration total ammonia nitrogen(TAN). At the superficial air velocity of 0.83 cm/sec, TAN removal rate and removal efficiency was $316.6{\pm}7.2g/m^3{\cdot}day$ and $92.8{\pm}2.2%$ respectively. Removal rate was continuously increased with decreasing hydraulic residence time(HRT) from 0.5 hr to 0.05 hr, whereas removal efficiency decreased with decreasing HRT. The optimum temperature for nitrification was $30^{\circ}C$ at which removal efficiency was $95.5{\pm}1.5%$. Nitrification was effectively performed at low temperature, $10^{\circ}C$. In the pH range from 7 to 9 in the bioreactor, removal rate and removal efficiency was $310{\pm}10g/m^3{\cdot}day$ and $94{\pm}3%$ respectively.