• Title/Summary/Keyword: airlift

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Evaluation of L-Lactic Acid Production in Batch, Fed-batch, and Continuous Cultures of Rhizopus sp. MK-96-1196 Using an Airlift Bioreactor

  • Liu, Tiejun;Miura, Shigenobu;Arimura, Tomohiro;Tei, Min-Yi;Park, Enoch Y.;Okabe, Mitsuyasu
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.522-527
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    • 2005
  • Various processes which produce L-lactic acid using ammonia-tolerant mutant strain, Rhizopus sp. MK-96-1196, in a 3L airlift bioreactor were evaluated. When the fed-batch culture was carried out by keeping the glucose concentration at 30g/L, more than 140 g/L of L-lactic acid was produced with a product yield of 83%. In the case of the batch culture with 200g/L of initial glucose concentration, 121g/L of L-lactic acid was obtained but the low product yield based on the amount of glucose consumed. In the case of a continuous culture, 1.5g/L/h of the volumetric productivity with a product yield of 71% was achieved at dilution rate of $0.024\;h^{-1}$. Basis on these results three processes were evaluated by simple variable cost estimation including carbon source, steam, and waste treatment costs. The total variable costs of the fed-batch and continuous cultures were 88% and 140%, respectively, compared to that of batch culture. The fed-batch culture with high L-lactic acid concentration and high product yield decreased variable costs, and was the best-suited for the industrial production of L-lactic acid.

A Study on Performance Characteristics of Small Airlift Pump (소형 에어리프트 펌프의 성능특성에 관한 연구)

  • Oh, S.K.;Lee, G.Y.
    • Journal of Power System Engineering
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    • v.4 no.3
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    • pp.34-39
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    • 2000
  • Performance data in the literature on air lift pumps have been based primarily on pumps of long length and large diameter (high lift pumps). Since mariculture operations involve pumps of relatively short length and small diameter, performance data are required for efficient operation. To provide such data, an experimental apparatus was designed and fabricated to test all lift pumps from 2.1 to 3.4 cm inside diameter and from 40 to 300 cm in length. Instrumentation was provided to measure water flow rate and air flow rate as well as water temperature, air temperature, and pressure throughout the system. Results from this study correlate well with high lift pump data in that, for a given pump geometry, maximum water flow occurs for a specific air flow rate. Driving the pump with air flows larger or smaller than this optimum flow rate will decrease the pumping rate. The optimum flows are significantly different for low lift pumps compared to high lift pumps. However, the pumping rate for low lift pumps approaches that for high lift pumps with increasing length.

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Gas and Liquid Flow Characteristics in an Internal Circulation Airlift Reactor using a Single Nozzle -Effects of Flow Zone Sizes- (단일노즐을 사용한 내부순환 공기리프트 반응기에서 기체 및 액체의 유동특성 - 유동지역의 크기영향 -)

  • Jang, Sea-Il;Kim, Jong-Chul;Jang, Young-Joon;Son, Min-Il;Kim, Tae-Ok
    • Applied Chemistry for Engineering
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    • v.9 no.6
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    • pp.901-906
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    • 1998
  • Gas and liquid flow characteristics were investigated in an internal circulation airlift reactor using a single nozzle for a gas distributor. In three reactors with different diameters of the downcomer and heights of the riser, the gas holdup in the individual flow zone and the impulseresponse curve of tracer for an air-water system were measured for various gas velocities and reactor heights. Experimental results showed that the flow behavior of bubbles in the riser was the slug flow due to strong coalescences of bubbles and that the bubble flow pattern in the downcomer was the transition bubble flow for the smaller diameter of the downcomer, however, it was the homogeneous bubble flow for the larger one. And mean gas holdups in the individual flow zone and the reactor were greatly increased with decreasing the diameter of the downcomer for the equal ratio of height of the top section to that of the riser. Also, the mixing time was much effected by the height of the top section of reactor and for the equal ratio of height of top section to that of the riser, it was increased with increasing the diameter of the downcomer and the height of the riser. Flow characteristics of liquid were mainly varied with the bubble flow pattern in the downcomer and the size of the top section of reactor. And circulation velocities of liquid in the riser were increased with increasing gas velocities and the size of the top section of reactor, and for the equal ratio of height of top section to that of the riser, they were increased with increasing the diameter of the downcomer and the height of the riser.

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The Simultaneous Nitrification and Organics Oxidation of Wastewater in Airlift Biofilm Reactors (공기리프트 생물막 반응기에서의 폐수 질화 및 유기물 동시산화)

  • 서일순;허충희
    • KSBB Journal
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    • v.16 no.6
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    • pp.573-578
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    • 2001
  • The effects of organic supplement (acetate) and dissolved oxygen concentration on the nitrification rate of wastewater were investigated in the 27.7 L pilot-scale airlift biofilm reactor with the granular activated carbon media of 0.613 mm diameter. The ammonium oxidation rate increased stepwise up to 5 kg N/㎥$.$d at the riser air velocity of 0.063 m/s, when the air velocity and the ammonium loading rate were raised alternately. The nitrite build-up was observed during the early stage of the biofilm formation, which disappeared after the reactor operation of 128 days. As increasing the organic loading rate, the organic oxidation rate increased up to 25.0 kg COD/㎥$.$d with the removal efficiency of 94% but the oxidation rates of ammonium and nitrite decreased. The oxidation rates of ammonium and nitrite increased with the dissolved oxygen concentrations. When the pure oxygen was sparged, the ammonium oxidation rate was almost five times higher than that with air at the same velocity.

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A Study on the Distribution of Nitrite Oxidation Microorganisms in a Biofilm Reactor

  • Yoon, Joung-Yee;Kim, Sun-Hee;Kim, Dong-Jin
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.282-286
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    • 2005
  • Biofilm airlift reactor was continuously operated to investigate the competitions between the autotrophs and heterotrophs, ammonia oxidizers and nitrite oxidizers, and Nitrobacter and Nitrospira with real wastewater at a C/N ratio of 0.86. As the reactor achieved complete nitrification microbial distribution was analyzed by FISH/CLSM technique. The results showed that heterotroph was more abundant than nitrifying bacteria. Ammonia oxidizers (17%) and Nitrobacter (7%) prevailed nitrite oxidizers (9%) and Nitrospira (2%), respectively.

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Performance of Airlift Pumps for Water Circulation and Aeration (물 순환 및 에어레이션용 에어리프트 펌프의 성능)

  • OH Se-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.6
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    • pp.529-537
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    • 1992
  • Airlift pumps were tested to evaluate their pumping and aeration capacities. The pumps were 34.5 inch long made of 2, 3, 4 and 6 inch nominal diameter PVC pipes. An one hp air blower was used to supply the air. The air-flow rate was measured by an anemometer type air-flow meter and air pressure was level changes in a water tank from which water was pumped. Aeration by the pumps was tested by the standard aeration test method with the center of pump outlet positioned 3 inches above water surface. Oxygen concentrations in water were measured to determine aeration rate. As pumping head increased by water level draw-down in the tank water flow decreased while air flow increased. The reduction rate of water flow was higher with 4 and 6-inch pumps. Small pumps showed very minor changes in the reduction. Aeration rates were similar among 3, 4, and 6 inch pumps. With one hp air blower 6-inch pump at the minimum pumping head achieved the best performance in terms of water circulation.

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Salt Acclimation Behavior of the Nitrifier Consortium for the Nitrification of Saline Wastewater

  • Seo, Jae-Koan;Kim, Sung-Koo
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.590-593
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    • 2001
  • The effect of salinity on the nitrification efficiency of the nitrifier consortium was evaluated for the nitrification of saline wastewater. The nitrifier consortium, which was the activated sludge acclimated with ammonium as the only energy source, was used as the nitrifier for the salt acclimation. Airlift reactors for the nitrification of ammonia with increasing concentration in saline synthetic wastewater (35 g/I NaCD, and synthetic wastewater without salt as a control, were continuously operated with the nitrifier consortium for 43 days. The ammonia removal rate was about 23g ammonia-N/$m^3$/day in both the absence and presence of the salt. An accumulation of nitrite was observed in the saline nitrification reactor at an early period. However, the nitrite decreased to less than 1 mg/l after 39 days of operation. The salinity increased the acclimation time of the nitrifier consortium to obtain a stable marine nitrification system. However, the salt acclimated system showed the efficient removal of ammonia which was same as that without salt.

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Condition of Exo-polysacchride Production from Submerged Mycelial Culture of Ganoderma lucidum by Using Air-lift Fermenter System (Air-lift Fermenter System을 이용한 Ganoderma lucidum 균사체의 심부배양에 의한 세포외 다당류의 생산 조건)

  • 이신영;강태수;이만춘
    • KSBB Journal
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    • v.13 no.5
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    • pp.547-553
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    • 1998
  • For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

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Removal of NH(sub)3-N by Using Immobilized Nitrifier Consortium in PVA[Polyvinyl Alcohol]-I. Effect of Packing Fraction and Aeration Rate on Ammonia Nitrogen Removal (PVA에 고정화된 질화세균군에 의한 암모니아성 질소 제거 I. 충진율 및 공기 유입량이 암모니아성 질소제거에 미치는 영향)

  • 서근학;김병진;오창섭
    • KSBB Journal
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    • v.16 no.3
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    • pp.314-319
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    • 2001
  • A nitrifier consortium immobilized in polyvinyl alcohol was used to remove ammonia nitrogen from synthetic wastewater in an airlift bioreactor. The minimum aeration rates were 0.4, 0.6, 0.8 and 1.0 vvm for 5, 10, 15 and 20% immobilized bead packing volume fraction, respectively. The efficient packing fraction and the aeration rate for ammonia nitrogen removal were 15% and 2.4 vvm, respectively. With a hydraulic retention time of 0.5hr, the removal rate and the efficiency of ammonia nitrogen removal were 1685 g/㎥$.$day and 48% at an influent ammonia nitrogen concentration of 75 g/㎥.

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