• Title/Summary/Keyword: agricultural plants

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Enhancement of Virus-induced Gene Silencing in Tomato by Low Temperature and Low Humidity

  • Fu, Da-Qi;Zhu, Ben-Zhong;Zhu, Hong-Liang;Zhang, Hong-Xing;Xie, Yuan-Hong;Jiang, Wei-Bo;Zhao, Xiao-Dan;Luo, Yun-Bo
    • Molecules and Cells
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    • v.21 no.1
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    • pp.153-160
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    • 2006
  • Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDS-inoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature ($15^{\circ}C$) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.

ANALYSIS OF WATER STRESS OF GREENHOUSE PLANTS USING THERMAL IMAGING

  • K. H. Ryu;Kim, G. Y.;H. Y. Chae
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11c
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    • pp.593-599
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    • 2000
  • Accurate quantification of plant physiological properties is often necessary for optimal control of an automated greenhouse production system. Conventional crop growth monitoring systems are usually burdensome, inaccurate, and harmful to crops. A thermal image analysis system was used to accomplish rapid and accurate measurements of physiological-property changes of water-stressed crops. Thermal images were obtained from several species of plants that were placed in a growth chamber. Analyzing the images provided the pattern of temperature changes in a leaf and the amount of differences in the temperature of stressed plants and non-stressed plants.

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Endophytic fungi harbored in Chinese native gramineous plants

  • Wang, Zhiwei;Ji, Yanling
    • 한국균학회소식:학술대회논문집
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    • 2009.10a
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    • pp.44-56
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    • 2009
  • $Epichlo\ddot{e}$ endophytes, including Neotyphodium spp. and $Epichlo\ddot{e}$ spp., enhance plant growth, mediate more plant tolerance or resistance to biotic and abiotic stresses, and also synthesis various biologically active compounds in their host plants, and important in many areas. In early stages, most of $epichlo\ddot{e}$ endophytes were described during surveys practiced by American, European and Oceania scientists, while fungal endophytes within native Asian plants were poorly investigated. In recent years, an $Epichlo\ddot{e}$ sp. and 4 Neotyphodium spp. were described in cool season Chinese native gramineous plants. Most of Chinese native Neotyphodium spp. were presumed as hybrids originated from members of ETC and EBY. Investigation on NRPS genes shows lack of toxic ergopeptines and potential production of peramine. Biological and ecological roles of Chinese native $epichlo\ddot{e}$ endophytes should be investigated in future, and it will be very valuable if we can have some joint projects with Korean scientists for Asian native $epichlo\ddot{e}$ endophytes.

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Genotoxicity of the Herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D): Higher Plants as Monitoring Systems

  • Enan, Mohamed R.
    • Journal of Forest and Environmental Science
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    • v.25 no.3
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    • pp.147-155
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    • 2009
  • Higher plants provide valuable genetic assay systems for screening and monitoring environmental pollutants. They are now recognized as excellent indicators of mutagenic effects of environmental chemicals and are applicable for the detection of environmental mutagens both indoor and outdoor. 2,4-dichlorophenoxyacetic acid (2,4-D) is a herbicide commonly used in agriculture. The residues of 2,4-D are present in air, water, soil and edible plants. It constitutes a real hazard to the public health because it's wide spread use in agriculture. Genotoxic effects of 2,4-D on plant cells and potential of higher plants as a biomonitoring system for detecting chemical mutagens are evaluated. It is recommended that higher plant systems have been accepted by regulatory authorities as an alternative biomonitoring system for the detection of possible genetic damage resulting from pollution and the use of environmental chemicals.

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GC/MS analysis of volatile constituents from woody plants (목본성 식물의 휘발성 성분 GC/MS 분석)

  • Lee, Dong-Gu;Choi, Kyung;Lee, Sang-Hyun
    • Korean Journal of Agricultural Science
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    • v.38 no.4
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    • pp.723-730
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    • 2011
  • To search for the new development of industrial application of woody plants, the chemical composition of the volatile constituents from woody plants (Evodia daniellii, Clerodendron trichotomum, Prunus padus, and Zanthoxylum ailanthoides) was determined by GC and GC/MS spectrometric analysis with the aid of NBS, Wiley Library and RI indice searches. The major constituents were t-ocimene from the leaves of E. daniellii, linalool from the leaves of C. trichotomum, benzaldehyde from the leaves and twigs of P. padus, ${\beta}$-thujene from the leaves of Z. ailanthoides, and 2-undecanone from the stems of Z. ailanthoides. These results suggested that the major volatile constituents of woody plants could be a useful lead compound in the development of functional materials for industrial application.

Screening of aldose reductase inhibitory activities of Korean folk plants in Chungcheong Province

  • Lee, Dong Gu;Lee, Ki Ho;Choi, Kyung;Ku, Jajung;Park, Kwang-Woo;Cho, Eun Ju;Lee, Sanghyun
    • Korean Journal of Agricultural Science
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    • v.40 no.1
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    • pp.47-51
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    • 2013
  • To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants in Chungcheong Province was investigated. Among Korean folk plants tested, the MeOH extract of Persicaria longiseta showed highest inhibition of AR ($IC_{50}$ value, $5.14{\mu}g/ml$). Consequently, P. longiseta has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.

Pharmacologic evalution of some anti-cancer plants from Paikdooshan and their application

  • Kim, Soo-cheol;Lee, Sang-Rea;Harunori Ooda;Lee, Jong-Ill
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11a
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    • pp.37-42
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    • 2002
  • Various kinds of plants were collected all over the Tsangbaishan from 1956-2000 . they were identified and classified into anticancer plants. According to the result they were composed of 61 families, 129 genera and 162 species. Among them, 10 species of anticancer plants is discussed about their pharmacologic evalution and application.

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