• Korean Journal of Environmental Agriculture
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• v.23 no.3
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• pp.158-169
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• 2004

For the improvement of gas chromatographic analysis of multiple pesticide residues in green pepper, lettuce and Chinese cabbage, multiresidue test mixtures (MRTMs) of 10 groups (ECD 5 groups and NPD 5 groups) and a recovery test mixture (RTM) of 18 compounds (11 compounds for ECD and 7 compounds for NPD) were established based on retention time and response to relevant detectors. A new extraction solvent (acetone: acetonitrile=1 : 9) and a clean up eluent (hexane: dichloromethane : acetonitile = 50 : 48.5 : 1.5) for solid-phase extraction (SPE) cartridge were selected to test two types of multiresidue methods (MRM I and MRM II). MRM II provided high recovery better than MRM I when RTM was tested Recovery experiment with MRTMs which was conducted using MRM II resulted in that more than seventy percents of compounds were recovered in the range of $50{\sim}140%$, while 9% of compounds were over 140% of recovery and only $7{\sim}8$ compounds failed to detect. MRM II, an improved method, could be employed for screening residues of 190 pesticides in those vegetables.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.467-469
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• 2003

This research aimed to develop forest type classification technique for the mixed forest with coniferous and broad-leaved species using the high resolution satellite data. QuickBird data was used as satellite data. The method of this research was to extract satellite data for every single tree crown using image segmentation technique, then to evaluate the accuracy of classification by changing grouping criteria such as tree species, families, coniferous or broad-leaved species, and timber prices. As a result, the classification of tree species and families level was inaccurate, on the other hand, coniferous or broad-leaved species and timber price level was high accurate.

• Archives of Pharmacal Research
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• v.26 no.8
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• pp.649-652
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• 2003

Polymeric nanoparticles composed of polystyrene (PS) as core and poly(methacrylic acid) (PMA) as corona were prepared by the dispersion copolymerization. The potential of the nanoparticles as carriers for recombinant human epidermal growth factor (EGF) was investigated. The nanoparticles showed monodispersity and good water-dispersibility. The loading content of EGF to the nanoparticles was very high due to electrostatic interaction between EGF and nanoparticles. EGF was released as a pseudo-zero order pattern after initial burst effect. The nanoparticles were sufficient for A431 cells proliferation.

• Journal of Agricultural Extension & Community Development
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• v.6 no.1
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• pp.35-42
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• 1999

Despite salary is one of the important factors affecting the morale, most of agricultural extension officials are not satisfied with salary system for their roles in Korea In these conditions, it is impossible to develop the extension services in Korea. It is necessary to improve salary system by upgrading to the level of salary for research officials or converting to the salary system for education officials such as professor, high school teach. The other alternative is to improve by reforming based on the tenure.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1285-1291
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• 2006

The proline utilization (put) operon of Vibrio vulnificus consists of the putAP genes encoding a proline dehydrogenase and proline permease. The result of put-lux transcriptional fusion analysis suggests that the V vulnificus putAP operon is not autoregulated by the PutA protein. A putR null mutation decreased proline dehydrogenase activity and the level of the put transcripts, indicating that transcription of putAP is under the positive control of PutR. The deduced amino acid sequence of the putR was similar to those reported from other bacteria with high levels of identity. Chromatin IP and GST pull-down assays revealed that PutR specifically binds to the putAP promoter region in vivo, and interacts with CRP in vitro. Taken together, the results suggested that PutR exerts its effect on putAP expression by directly interacting with CRP bound to the upstream region of P$_{put}$.

• Journal of Korean Society of Rural Planning
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• v.13 no.1 s.34
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• pp.51-62
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• 2007

This study examines empirically the characteristics of agricultural corporations in terms of their market and technology environment and the efficiency of the governmental support to the firms. Also the study analyzes the moderating effect of the environmental characteristics on the relationship between governmental supports and firm performance based on the concept of balanced performance. Test results show that firm tend to evaluate favorably their technology and market competitiveness of product quality and price and to require tools for reaction to changes of customer needs and technology or competing products and external corporation that is necessary for long-term technological competitiveness. As for governmental support, strategic support and service is much more appreciated than direct individual supporting item. Agricultural corporations with unfavorable market environment evaluate relatively high on their balanced performance. Correlations of balanced performance factors and technology environmental factors show all positive statistical significance. The moderating effect of the environmental characteristics on the relationship between governmental support and balanced performance can be interpreted that the support is provided without considering environmental aspect and based only on financial performance and as a result not to be satisfied by agricultural corporations with technological competitiveness.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.556-563
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• 2019

Objective: This experiment was conducted to investigate the effect of reducing dietary metabolic energy (ME) and crude protein (CP) levels on growth performance, blood profiles, and nutrient digestibility in weaning pigs. Methods: A total of 240 crossbred pigs ($Duroc{\times}[Landrace{\times}Yorkshire]$) with an average body weight of $8.67{\pm}1.13kg$ were used for a 6-week feeding trial. Experimental pigs were allotted to a $2{\times}3$ factorial arrangement using a randomized complete block design. The first factor was two levels of dietary ME density (low ME level, 13.40 MJ/kg or high ME level, 13.82 MJ/kg) and the second factor was three dietary CP levels based on subdivision of early and late weaning phases (low CP level, 19.7%/16.9%; middle CP level, 21.7%/18.9%; or high CP level, 23.7%/20.9%). Results: Over the entire experimental period, there were no significant difference in body weight among groups, but a decrease in diet energy level was associated with an increase in average daily feed intake (p = 0.02) and decrease in gain-feed ratio (G:F) ratio (p<0.01). Decreased CP levels in the diet were associated with a linear increase in average daily gain (p<0.05) and quadratic increase in G:F ratio (p<0.05). In the early weaning period, blood urea nitrogen concentration tended to increase when ME in the diet decreased and decrease when CP level in the diet decreased (p = 0.09, p<0.01, respectively). Total protein concentration tended to increase when CP level was reduced (p = 0.08). In the late weaning period, blood urea nitrogen concentration decreased linearly as CP level decreased (p<0.01). The CP and crude fat digestibility decreased when ME was decreased by 0.42 MJ/kg (p = 0.05, p = 0.01, respectively). The CP digestibility increased linearly as CP level decreased (p = 0.01). Conclusion: A weaning pig diet containing high ME level (13.82 MJ/kg) and low CP level (19.7%/16.9%) can improve pig growth performance and nutrient digestibility.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1276-1284
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• 2006

Listeria monocytogenes is a well-known high-risk foodborne pathogen that grows at refrigeration temperature and is responsible for outbreaks of listeriosis. We report here the incidence of L. monocytogenes in fresh chicken carcasses and present genetic diversity of L. monocytogenes isolates. In this study, 25 g of chicken carcasses from markets in Korea were examined according to the FDA method, and presumptive isolates were confirmed by multiplex PCR assay. L. monocytogenes isolates were analyzed by Pulsed-Field Gel Electrophoresis using restriction enzymes, ApaI and AscI, to obtain strain-specific DNA fragments profiles. Antimicrobial resistance of L. monocytogenes strains against generally used antibiotics (Penicillin G, Kanamycin, Tetracycline, Vancomycin, Cephalothin, Rifampicin, Erythromycin, Ampicillin, Gentamicin, Streptomycin, and Chloramphenicol) were analyzed by NCCLS protocols to examine the presence of antimicrobial resistance in natural L. monocytogenes. Of a total 274 chicken samples, 81 samples (29.6%) were positive for L. monocytogenes. Listeria innocua (50.1%), Listeria welshimeri (6.9%), and Listeria grayi (11.3%) were also detected. PFGE analysis, using restriction enzymes ApaI and AscI, showed 27 pulsotypes of L. monocytogenes. Antimicrobial resistance analysis confirmed the existence of antimicrobial resistance for penicillin G and tetracycline in isolated L. monocytogenes strains.

• Korean Journal of Agricultural Science
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• v.48 no.1
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• pp.141-149
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• 2021

The cultivation area for domestic cabbage increased by 26.3% from 10,968 ha in 2019 to 13,854 ha in 2020, and among leafy vegetables, the cabbage cultivation area was 62%, and production was 78.9%. Demand for field crop production of cabbage, which has a relatively high-income level compared to rice farming, is increasing, and mechanization of the field operation is urgently needed due to the insufficient development of related farming machinery. In this study, a prototype fabrication and performance test of a tractor-attached cabbage collector was carried out. The transport section was divided into two parts, one for the feeding and transportation and the other for the screening and packaging to selectively collect cabbages in bulk bags or boxes. The length of the primary collecting conveyor was designed to meet the field conditions of the Korean cabbage cultivation standards so that six cultivation rows could be worked simultaneously. Power was controlled by a hydraulic transmission line of the tractor and was easily mounted onto the 3-point hitch links behind the tractor. When the performance was evaluated, the transfer rate, loss rate, damage rate, and work performance were 100, 0, 1.2%, and 1.9 h·10 a^-1. Final improvement and commercialization of the prototype would considerably contribute to the mechanization of harvesting cabbage, the main ingredient of Kimchi.

• The Korean Journal of Community Living Science
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• v.19 no.4
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• pp.619-640
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• 2008

The objective of this study is to promote alternative plans on the prejudiced and distorted parts in school textbooks in the aspect of multi-functionality of agriculture and rural community. This was done through analysis of the contents in 44 kinds of schoolbooks in Korean language (30 of elementary school, 12 of middle school and 2 of high school). This will lead to promote the understanding about the multi-functionality and form the proper value system related to agriculture and rural community for children and juveniles using schoolbooks. A content analysis which is a research tool to classify all contents of materials containing the properties to become the object of interest in a systematic way, was employed to examine to what extent the contents on agriculture and rural community were reflected in the textbooks for elementary school, middle school and high school. The content analysis was done in two categories, namely; application method and function view. Application method had four types of texts, cases, figures pictures, and activities, while function view emphasized the relevance for the multi-functionality of agriculture and rural community (i.e., function of environment preservation, function of rural scenery and rural traditional culture conservation, function of maintenance and development of local society, and function of food security). The results of application method in elementary school showed that the textbook of the second-year class having a large amount of figures pictures related to agriculture and rural community, had the highest frequency in all six grades. In the function view, the environment preservation function was most abundant among a variety of multi-functionalities. In middle school, the average frequency had shown a rapid decrease. However, the function of environment preservation in the function view and the texts by application method were most frequent. in the first-year class. In high school, however, any mention related to agriculture and rural community was not made, in spite of the fact that the ten parts of textbook contained a lot of contents with a reasonable level. Based on the results of content analysis, findings and recommendations by part in textbooks for elementary school, middle school and high school have been drawn, and several examples for the content development to be included in the textbooks were listed.