• International Journal of Industrial Entomology and Biomaterials
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• v.39 no.1
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• pp.22-28
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• 2019

We previously isolated 9 clones that show stronger signal compared to Bombyx mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid similarity with ${\beta}$-tubulin gene of B. mori. This clone was ubiquitously expressed in all tissues and developmental stage of B. mori. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-750/-1) in the 5'-flanking region of ${\beta}$-tubulin gene, which has about 47 fold more intensive promoter activity than BmA3 promoter. Moreover, the ${\beta}$-tubulin promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that ${\beta}$-tubulin promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.

• Journal of agricultural medicine and community health
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• v.42 no.2
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• pp.87-96
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• 2017

Objectives: We studied the relationship between health behavior and subjective unhappiness in high school students. Methods: Using 27,097 responses from the 2015 Korea Youth Risk Behavior Web-based Survey for general high school students. we analyzed by multiple logistic regression based on the complex sample design Results: Unhappiness was positively related with the low economic status, smoking, drinking, fast foods intake, and negatively related with fruit intake in results of multiple logistic regression. Conclusion: Health behaviors have a significant impact on the unhappiness of high school students. Therefore, in-depth research and policies to decrease unhappiness of high school students through health promotion are required.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.700-704
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• 1998

Each of the recombinant structural genes of Helicobacter pylori urease, ureA and ureB, was cloned and overexpressed as inclusion bodies. Solubilization and renaturation of the inclusion bodies were carried out, to accelerate the pairing of sulfhydryl groups and the incorporation of nickel ions, which would lead to the native structure with high enzyme activity. Rates of urea hydrolysis were monitored as an indication of in vitro activation of renatured ureases. The activation of the apoprotein using 1 mM nickel ion, 100 mM sodium bicarbonate and a 10:1 ratio of reducing power resulted in a weak urease activity (about 11% of the native urease activity encoded by pTZ 19R/ure-l). When a sparse matrix screen method originally discovered for the crystallization of proteins was used, the activity increased higher than that obtained using glutathione. The effect of polyethylene glycol (PEG) on the activity was noticeable, giving two-fold increase in the specific activity (about 11 U/mg of protein corresponding to 22% of the native urease activity encoded by pTZ19R/ure-1).

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.3
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• pp.305-308
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• 2004

The purpose of this study was to estimate the genetic parameters for backfat thickness at shoulder, mid back and loin and days to 90 kg using a derivative-free REML procedure. Data were collected from 6,146 boars and gilts of purebred Durocs, Landraces and Large Whites performance tested at breeding farms of National Agricultural Cooperatives Federation in Korea from 1998 to 2001. Estimated heritability for backfat measurements at shoulder, mid-back and loin and an average of those backfat measurements were 0.14, 0.32, 0.22 and 0.25 in Durocs, 0.34, 0.50, 0.42 and 0.46 in Landraces and 0.33, 0.52, 0.43 and 0.49 in Large Whites. Heritabilities of backfat measurements estimated were hightest in mid-back and lowest at shoulder. Phenotypic variances of backfat measurements estimated were largest at shoulder and smallest at mid-back. Estimated heritabilities for days to 90 kg were 0.37 in Durocs, 0.42 in Landraces and 0.54 in Large Whites. Genetic correlations among backfat measurements at shoulder, mid-back and loin and an average of those backfat measurements estimated were positive and very high. Genetic correlations of days to 90 kg with the backfat measurements estimated were _0.19 ~ _0.30 in Durocs, _0.04 ~ _0.17 in Landraces and _0.10 ~ _0.13 in Large Whites.

• The Korean Journal of Food And Nutrition
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• v.23 no.2
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• pp.269-275
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• 2010

The current exploratory study attempted to investigate the influence of environment-friendly agricultural products(EAPs) perception on EAPs consumption behavior. The survey was conducted to parents of elementary school in Chung-buk area by the help of homeroom teachers of each school. Out of 500 questionnaires administered, a total of 305 completed questionnaires were returned, yielding a response rate of 61%. Regarding buying behavior, 89.6% of respondents have ever bought EAPs, among them, 52.9% usually bought in a mart. The reason to buy EAPs was mostly safety(57.6%), nutrition & health(30.4%), and quality(7.0%). The 11 items representing the attitude and knowledge were analyzed, resulting in three distinct dimensions-'attitude', 'knowledge', and 'concern'. The knowledge level of EAPs was slightly higher than medium. Concerning the influence of EAPs perception on EAPs consumption behavior, the more having high knowledge and attitude level on EAPs, the more lead to the EAPs consumption behavior. This implicit the importance of education to enhance the knowledge level and positive attitude towards EAPs.

• Journal of Biosystems Engineering
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• v.25 no.6
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• pp.445-456
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• 2000

A can type high-speed transplanting mechanism was developed. The mechanism consists of a cam and an epicycle gear train with one sun gear in the middle and four planet gears in a row but symmetric with respect to the sun gear. Two planting knives are pivoted on the two outer gears. When sun gear rotates with a constant velocity the planting knife rotates also with a constant velocity. This constant motion of the transplanting knife is accelerated partially by a cam fixed in the gear housing so that the locus of the planting knife becomes similar to that generated by a crank-type transplanting mechanism. This cam-type transplanting mechanism can solve the problems associated with the rotary-type transplanting mechanisms. The mechanism was designed with an aid of computer simulation and proved applicable to high speed transplanters by its mock-up model. The design process of the mechanism was presented and dynamic analysis was also carried out to show the advantages of the mechanism over the rotary type high-speed planting mechanism.

• Korean Journal of Food Science and Technology
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• v.52 no.4
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• pp.363-368
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• 2020

This study was conducted to evaluate several physiological activities of 4 sweet persimmon cultivar leaf water extracts. One new cultivar ('Hongchu' (HC)]) and three traditional cultivars ('Sangseojosaeng' (UW), Japanese Uenishiwase; 'Seochonjosaeng' (NW), Japanese Nishimurawase; and 'Buyu' (FY), Japanese Fuyu) were used in this study. The HC extract showed significantly higher DPPH and ABTS radical scavenging activities, tyrosinase inhibitory activity, and acetaldehyde dehydrogenase activity than the other extracts, while the FY extract exhibited a relatively higher alcohol dehydrogenase activity. The HC extract contained higher amount of phenolics and ascorbic acid. These results suggest the possibility of high-quality persimmon leaf tea development using the new sweet persimmon cultivar, HC.

• Agricultural and Biosystems Engineering
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• v.4 no.2
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• pp.50-57
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• 2003

This study was conducted to develop software which provides automatic site-specific field data acquisition, data processing, data mapping and management for precision agriculture. The developed software supports acquisition and processing of both digital and analog data streams. The architecture was object-oriented and each component in the architecture was developed as a separate class. In precision agriculture research, the laborious task of manual ground-truth data collection will be avoided using the developed software. In addition, gathering high-density data eliminates the need for interpolation of values for un-sampled areas. This software shows good potential for expansion and compatibility for variable-rate-application (VRA). The FIM (Field Information Management) computer program provides the user with an easy-to-follow process for field information management for precision agriculture.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.1
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• pp.60-64
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• 1998

The different developmental stage embryos and oviductal and uterine fluids of rabbit were analyzed by gas chromatography. Myristic (C 14:0), palmitic (C 16:0), palmitoleic (C 16:1), stearic (C 18:0), oleic (C 18:1), linoleic (C 18:2), linolenic (C 18:3), arachidic (C 20:0), arachidonic (C 20:4), docosahexaenoic (C 22:6) and lignoceric (C 24:0) acids were the common fatty acid constituents with little exception. In most of the samples palmitic, oleic, linoleic and arachidonic acids were observed in high concentration. Moreover, linoleic, linolenic and arachidonic acids were the three poly-unsaturated fatty acids in both type sample except day-1 oviductal fluids. Similarly, in both day-1 and day-2 oviductal and uterine fluids myristic, palmitoleic, stearic, linolenic, arachidic and docosahexaenoic acids were in less composition or undetected.

• Journal of Biosystems Engineering
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• v.27 no.6
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• pp.513-520
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• 2002

A 5-row walking garlic harvester with pulling mechanism was developed in the study, based upon a kinematical analysis and related preliminary field tests on the conventional garlic harvesting method. The harvesting efficiency of the developed harvester was more than 98% in the garlic field irrigated before harvesting, 80-85% in the field without irrigation. The harvesting performance of the harvester was 660∼825㎡/hr at the forward speed of harvester of 0.3m/s in the irrigated field before garlic harvesting. For proper garlic drying and collection, the harvester discharged the harvested garlic on the ground uniformly at the angles of 135。∼150。 to its forward direction with the garlic bulb's placing toward the harvester. In the field tests, it was recommended that the forward speed of the harvester be approximately less than 0.2m/s, and that the spacing of planting garlic seeds should be standardized in the future fur increasing its harvesting efficiency.