• Proceedings of the Korean Society of Sericultural Science Conference
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• 2004.10a
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• pp.44-44
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• 2004

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2005.11a
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• pp.44-44
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• 2005

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2005.11a
• /
• pp.46-46
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• 2005

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2005.11a
• /
• pp.45-45
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• 2005

• 한국균학회소식:학술대회논문집
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• 2009.10a
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• pp.41-43
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• 2009

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.94.1-95
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• 2002

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.139-139
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• 2020

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.147-147
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• 2020

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2057-2063
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• 2018

Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and $40^{\circ}C$. It exhibited $K_m$ and $V_{max}$ values of $0.565mmol\;l^{-1}$ and $1.51{\mu}mol\;l^{-1}\;min^{-1}$ for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.54-58
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• 2016

In natural conditions, the black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. This species colonizes and oviposits in a wide variety of decomposing vegetable and animal matter. For mass rearing of black soldier flies, the egg deposition methods are dependent on sunlight and oviposition sites. In this study, we investigated the substrates and oviposition sites preferred by black soldier fly in artificial rearing system. Our results showed that as black soldier flies prefer mating under sunlight, they prefer ovipositing at sites where adequate sunlight and food substrate are available. Further detailed research is required to develop methods for artificially rearing black soldier fly throughout the year in Korea.