• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.167-172
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• 2022

Pathophysiological reaction of platelets in the blood vessel is an indispensable part of thrombosis and cardiovascular disease, which is the most common cause of death in the world. In this study, we performed in vitro assays to evaluate antiplatelet activity of artemether in human platelets and attempted to identify the mechanism responsible for protein phosphorylation. Artemether is a derivative of artemisinin, known as an active ingredient of Artemisia annua, which has been reported to be effective in treating malaria, and is known to function through antioxidant and metabolic enzyme inhibition. However, the role of artemether in platelet activation and aggregation and the mechanism of action of artemether in collagen-induced human platelets are not known until now. In this study, the effect of artesunate on collagen-induced human platelet aggregation was confirmed and the mechanism of action of artemether was clarified. Artemether inhibited the phosphorylation of PI3K/Akt and Mitogen-activated protein kinases, which are phosphoproteins that are known to act in the signal transduction process when platelets are activated. In addition, artemether decreased TXA₂ production and decreased granule secretion in platelets such as ATP and serotonin release. As a result, artemether strongly inhibited platelet aggregation induced by collagen, a strong aggregation inducer secreted from vascular endothelial cells, with an IC₅₀ of 157.92 μM. These results suggest that artemether has value as an effective antithrombotic agent for inhibiting the activation and aggregation of human platelets through vascular injury.

• Journal of Korean Society of Transportation
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• v.35 no.1
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• pp.63-78
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• 2017

The purpose of this study is to determine the optimal aggregation interval to increase the reliability when estimating representative value of individual vehicle travel time collected by DSRC equipment in interrupted traffic flow section in National Highway. For this, we use the bimodal asymmetric distribution data, which is the distribution of the most representative individual vehicle travel time collected in the interrupted traffic flow section, and estimate the MSE(Mean Square Error) according to the variation of the aggregation interval of individual vehicle travel time, and determine the optimal aggregation interval. The estimation equation for the MSE estimation utilizes the maximum estimation error equation of t-distribution that can be used in asymmetric distribution. For the analysis of optimal aggregation interval size, the aggregation interval size of individual vehicle travel time was only 3 minutes or more apart from the aggregation interval size of 1-2 minutes in which the collection of data was normally lost due to the signal stop in the interrupted traffic flow section. The aggregation interval that causes the missing part in the data collection causes another error in the missing data correction process and is excluded. As a result, the optimal aggregation interval for the minimum MSE was 3~5 minutes. Considering both the efficiency of the system operation and the improvement of the reliability of calculation of the travel time, it is effective to operate the basic aggregation interval as 5 minutes as usual and to reduce the aggregation interval to 3 minutes in case of congestion.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.1
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• pp.35-40
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• 2011

In this paper, we compare and analyze data aggregation methods based on event area in wireless sensor networks. Data aggregation methods consist of two methods: the direct transmission method and the aggregation node method. The direct aggregation method has some problems that are data redundancy and increasing network traffic as all nodes transmit own data to neighbor nodes regardless of same data. On the other hand the aggregation node method which aggregate neighbor's data can prevent the data redundancy and reduce the data. This method is based on location of nodes. This means that the aggregation node can be selected the nearest node from a sink or the centered node of event area. So, we describe the benefits of data aggregation methods that make up for the weak points of direct data dissemination of sensor nodes. We measure energy consumption of the existing ways on data aggregation selection by increasing event area. To achieve this, we calculated the distance between an event node and the aggregation node and the distance between the aggregation node and a sink node. And we defined the equations for distance. Using these equations with energy model for sensor networks, we could find the energy consumption of each method.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.233-236
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• 1999

In order to clarify the anti-Ohyul activity of rhubarb, we investigated the effects of water extract from rhizomes of four different rhubarb on blood platelet aggregation induced by arachidonic acid, ADP, collagen and PAF in vitro. The cultivated Korean rhubarb rhizomes (Rheum undulatum) exhibited the most potent inhibitory action on the aggregation induced by arachidonic acid and also among the four fractions, stilbene components containing part showed strong inhibitory action. These inhibitory effect may partially contributed to anti-Ohyul activity of rhubarb.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2006.05a
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• pp.370-373
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• 2006

In this paper, we consider set-valued aggregation operators and investigate some properties of them. Moreover, we define interval-valued Choquet integral aggregation operators and discuss their characterizations.

• Journal of applied mathematics & informatics
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• v.19 no.1_2
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• pp.215-229
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• 2005

In this paper, constraint aggregation is combined with the adjoint and multiple shooting strategies for optimal control of differential algebraic equations (DAE) systems. The approach retains the inherent parallelism of the conventional multiple shooting method, while also being much more efficient for large scale problems. Constraint aggregation is employed to reduce the number of nonlinear continuity constraints in each multiple shooting interval, and its derivatives are computed by the adjoint DAE solver DASPKADJOINT together with ADIFOR and TAMC, the automatic differentiation software for forward and reverse mode, respectively. Numerical experiments demonstrate the effectiveness of the approach.

• Archives of Pharmacal Research
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• v.22 no.4
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• pp.401-403
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• 1999

In continued studies on cultivated Korean rhubarb rhizomes (Rheum undulatum), three known stillbenes (desoxyrhapontigenin, rhapontigenin, piceatannol) have been screened for activity on blood platelet aggregation. Both rhapontigenin and desoxyrhapontigenin exhibited strong inhibition on the aggregation induced by arachidonic acid collagen. However, piceatannol did not show inhibition. These inhibitory effects may partially contribute to anti-blood stagnancy activity of rhubarb.

• Biomedical Science Letters
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• v.10 no.1
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• pp.1-8
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• 2004

Cordycepin separated from Cordyceps militaris is a major physiologic active component in Cordyceps militaris. The platelet aggregation is stimulated by $Ca^{2+}$, which is either mobilized from intracellular endoplasmic reticulum or transported from extracellular space. cGMP antagonizes the actions of $Ca^{2+}$. Based on these facts, we have investigated the effects of cordycepin on the mobilization of $Ca^{2+}$ and the production of cGMP on collagen ($10\mu$g/ml)-induced human platelet aggregation. Cordycepin potently stimulated the human platelet aggregation induced by collagen ($10\mu$g/ml) in a dose-dependent manner. Cordycepin (500 $\mu$M) inhibited also the collagen-induced human platelet aggregation in the presence both 1 mM and 2 mM of $CaCl_2$. These are in accord with the results that cordycepin inhibited the $Ca^{2+}$- influx on collagen-induced human platelet aggregation. These results suggest that cordycepin decrease the intracellular $Ca^{2+}$ concentration to inhibit collagen-induced human platelet aggregation. Besides, cordycepin increased the level of cGMP on collagen-induced human platelet aggregation. This result is related with the decrease of intracellular $Ca^{2+}$ concentration, because cGMP inhibits the mobilization of $Ca^{2+}$. In addition, cordycepin inhibited the human platelet aggregation induced by LY -83583, inhibitor of guanylate cyclase. This result suggested that cordycepin inhibit the platelet aggregation by stimulating the activity of guanylate cyclase. In conclusion, we demonstrated that cordycepin might have the antiplatelet function by inhibiting $Ca^{2+}$-mobilization via the stimulation of the production of cGMP.

• Development and Reproduction
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• v.17 no.4
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• pp.409-420
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• 2013

Human serum (HS) has been reported to induce aggregation of human eyelid adipose-derived stem cells (HEACs) during high-density culture in vitro. The present study focused on the role of cell adhesion molecules and gelatinases during HS-induced aggregation of HEACs. HS-induced aggregation occurred between 9-15 days of culture. Cells aggregated by HS medium (HS-agg) showed stronger expression of ${\alpha}2$, ${\alpha}2B$, ${\alpha}X$, and CEACAM1 genes compared to non-aggregated cells in HS medium (HS-ex) or in control FBS-cultured cells. HS-agg were distinctly labeled with antibodies against ${\alpha}2$, ${\alpha}2B$, and ${\alpha}X$ proteins. Western blot results demonstrated that the two integrin proteins were greatly expressed in HS-agg compared to HS-ex and control FBS-cultured cells. Treatment of HEACs with anti-integrin ${\alpha}2$ antibody during culture in HS medium delayed aggregation formation. HS-agg exhibited strong expression of MMP1 and MMP9 compared to HS-ex or FBS-cultured cells. Conditioned media from HS-culture showed remarkable increase of MMP9 gelatinolytic activity in comparison to those from FBS-culture. However, there was no change of TIMP mRNA expression in relation to the HS-induced aggregation. Based on these results, it is suggested that integrin ${\alpha}2$, ${\alpha}2B$, and ${\alpha}X$, and MMP9 might play an important role in the HS-induced aggregation of HEACs.

• Journal of Korea Spatial Information System Society
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• v.9 no.3
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• pp.1-16
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• 2007

A spatial data warehouse is a system to support decision making using a spatial data cube. A spatial data cube is composed of a dimension table and a fact table. For decision support using this spatial data cube, the concept hierarchy of spatial dimension and the summarized information of spatial fact should be provided. In the previous researches, however, spatial summarized information is deficient. In this paper, the spatial aggregation for spatial summarized information in a spatial data warehouse is proposed. The proposed spatial aggregation is separated of both the numerical aggregation and the object aggregation. The numerical aggregation is the operation to return a numerical data as a result of spatial analysis and the object aggregation returns the result represented to object. We provide the extended struct of spatial data for spatial aggregation and so our proposed method is efficient.