• Laboratory Medicine Online
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• v.1 no.1
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• pp.64-66
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• 2011

Anti-Sd^a is of no clinical significance, because it rarely causes hemolytic transfusion reactions. Even when its presence is suspected during antibody screening test, further identification of the antibody is usually not performed. We experienced a case of anti-Sd^a in 73 yr-old male patient showing mixed field agglutination by microcolumn agglutination. Antibody specificity could not be identified by conventional antibody identification test, and it was proven to be anti-Sd^a by urine neutralization test. In spite of its little clinical significance, it may give incompatible crossmatching results reacting with Sd^a antigen, which occurs at a high frequency in general population. When incompatible crossmatch results arising from anti-Sd^a are suspected, the problem may be solved by using the urine-neutralized serum of in crossmatching test.

• Korean Journal of Veterinary Service
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• v.14 no.2
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• pp.104-109
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• 1991

Tile present study was carried out to investigate the incidence of bovine brucellsis in Cheju-do during the period from 1985 to 1990. The results were summarized as follows. 1. In the total 239,238 cattles tested. 1180(0.49%) were positive by standard tube agglutination test during the period from 1985 to 1990. 2. The major causes of incidence on brucellosis was grazing with carriers and repeated incidence in a herd. 3. The 13 Brucella abortus biotype 1 isolated from 10(50％) of 20 cattles slaughtered on brucellosis in 1990.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.859-866
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• 1993

With the object of doing mechanical grafting of cucurbitaceous vegetables, the grafting robot was developed in 1989. This robot consists of the following components : feeding wheels, grippers, conveying wheels, cutters , fixing clipper, discharger , controller and power supply. One cycle time to produce a grafted plant is about 3 seconds, Results of some cucumber grafting tests : successful grafting rate of 98 percent and after acclimation, a successful agglutination rate of 95 percent. These techniques are now transferring to the private company. The commercial robot will come into the market in this year.

• Annual Conference on Human and Language Technology
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• 1992.10a
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• pp.105-117
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• 1992

교착어(agglutinating language)에서는 다양한 통사정보가 독자적인 형태소에 내재되어 있다. 국어의 경우 형태소의 분석이 통사구조 분석에 선행되어 이루어져야 하는 이유가 바로 국어의 교착어적인 속성에 기인한다. 이 논문의 전반부에서 국어의 교착성을 명확히 보여주는 등위 접속구문을 핵심어 주도 구구조문법(HPSG)에 의하여 분석한다. 후반부에서는 PROLOG로 구현된 국어의 형태소 분석기와 통사구조 분석기(PARSER)를 소개한다.

• Korean Journal of Veterinary Research
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• v.18 no.1
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• pp.19-22
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• 1978

In order to isolate and identify Anaplasma bodies, two dairy cattle were inoculated with the whole blood of Korean cattle reacted to capillary tube agglutination test (Ana-test). The results obtained are as follows: 1. In the infected dairy cattle, Anaplasma bodies were first detected between 30 to 40 days post infection. 2. The isolates were identified as Anaplasma centrale on the basis of morphology of infected anaplasma bodies in erythocytes.

• Journal of Environmental Health Sciences
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• v.23 no.4
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• pp.45-49
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• 1997

Clostridium perfringens A형이 생산하는 장독소를 검색해본 결과, RPLA법에 있어서는 2배로 희석한 용액으로부터 64배로 희석한 용액 (NCTC 8239 Hobbs serotype 3 CPE$^+$)에서까지 양성반응을 보였으며 PCR 기법에 있어서는 10 pg 희석 용액까지 364 bp의 장독소 DNA fragment(NCTC 8238 Hobbs serotype 2 CPE$^+$)를 확인 할수 있었다. 그러므로 장독소를 검색하기 위해서는 PCR기법이 RPLA법에 비하여 훨씬 감도가 높음을 확인할 수 있었다.

• Korean Journal of Veterinary Service
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• v.23 no.4
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• pp.349-360
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• 2000

An epidemiological survey was conducted to investigate fowl typhoid outbreaks in Kyungnam province of Korea. The causative agent, salmonella gallinarum was isolated from 68 chicken samples of tentatively diagnosed fowl typhoid cases occurred during the period from January 1996 to September 1999. Comparative studies were also carried out to evaluate the diagnostic methods for detection of S gallinam The results obtained were as follows; 1. Of the 68 cases of tentatively diagnosed fowl typhoid, 56 (82%) cases were determined as fowl typhoid by biochemical test and pathological findings. The other 12 (18%) cases were determined as paratyphoid. 2. Fowl typhoid outbreaks occur continuously all seasons in the year, however the incidence was remarkably increased from May to September. 3. The frequency of incidence of fowl typhoid in terms of regional distribution was relatively high in egg-laying hens facilities, and the mode of transmission is likely to be either egg-to-egg or lateral transfer by wild birds or rats. 4. All of 18 isolates from 56 cases were identified as S gallinarum by biochemical and serological test. 5. Antimicrobial drug susceptibility test against 18 isolates showed that the isolates were highly susceptible to ASH, CZ, CF and GM (above 90%), whereas those strains were 100% resistant to EM, NA and PC. 6. S gallinarum rfbS gene was targeted to be amplified by PCR for comparative detection of S gallinarum in the experimentally infected chickens. The amplified 720bp DNA fragment, which is specific in D serogroup strains of S enterica subspecies was confirmed by agarose gel electrophoresis. 7. A comparison made between fecal culture and PCR-method revealed that later-method was relatively higher in detection rate than that of former method for S gallinarum. 8. Comparison of currently applied methods, rapid serum agglutination test (RST) and microplate agglutination test (MAT), with experimentally infected chickens were made to evaluate sensitivity of detection by neutralizing antibody titration. Both methods detected neutralizing antibodies from the challenged chickens of 5 day post infection. However, positive reactions were determined after 7 and 9 days post infection by MAT and RST, respectively.

• The Journal of the Korean Society for Microbiology
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• v.20 no.1
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• pp.25-34
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• 1985

Species of scotochromogenic mycobacteria of Group II isolated from sputa of patients with pulmonary tuberculosis and tuberculosis-like diseases from 1979 to 1984 were identified by simple biochemical tests using. nitrate reduction, Tween-80 hydrolysis, arylsulfatase and urease test, and serotypes of the isolates belonging to M. scrofulaceum were differentiated by bacterial agglutination test. Of 39 strains. tested, 11(28.2%) proved to be M. scrofulaceum, 15(38.5%) M. flavescens and 13(33.3%) M. gordonae. But none of the isolates belonged to M. szulgai and M. xenopi known as major pathogens of mycobacteria of Group II. Of 11 strains of the isolates identified as M. scrofulaceum 3 strains(27.3%) each belonged to serotype 41 and 42, and 4 strains(36.4%) belonged to serotype 43, but one strain was not typable because of its inagglutinability by any one of the type specific sera. In addition, the sensitivity and specificity of rabbit immune sera against type strains of serotype 41, 42 and 43 of M. scrofulaceum were analysed by bacterial agglutination test. In the sensitivity of microplate test with 11 isolates of M. scrofulaceum, a comparative tandem test using 2 units and one unit of absorbed antisera against three serotypes appeared to be superior to a conventional microplate test using one unit of type specific antisera.

• Parasites, Hosts and Diseases
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• v.38 no.4
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• pp.251-256
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• 2000

There have been some reports about the prevalence of anti-Toxoplasma gondii antibody among Koreans, and most of all data were taken from patients visiting hospitals. However, the epidemiological data of the community-based study in Korea are rare. This study was performed to evaluate the seroprevalence of toxoplasmosis among the inhabitants of the rural area Okcheon-gun, Korea. A total of 1,109 serum samples (499 males, 610 females) were examined for the IgG antibodies by ELISA. To set up the cut-off point for ELISA, we used a commercial latex agglutination (LA) kit. The sensitivity and specificity of ELISA against LA test were 89.5%, and 98.6% respectively. Among 1,109 sera, 6.9% showed seropositivity by ELISA. The positive rates of males and females were 6.0% and 7.2%, respectively. However, there were no significant differences between sexes. Comparing the age groups, the highest seropositive rate showed in the seventies or higher, and their rates had a tendency to increase with age (0.05 < p < 0.3). These results revealed that the seroprevalence of toxoplasmosis in rural inhabitants is similar to previous reports in Korea; however we need further investigation to clarify the prevalence of toxoplasmosis in the general population.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.511-525
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• 2007

A seroprevalence study of small ruminant brucellosis was conducted in sheep and goat rearing selected areas of Mymensingh district and Dhaka district, Bangladesh, from March, 2005 to May, 2006. Sera from 62 sheep and 300 goats were tested by rose bengal plate test (RBPT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 62 sera tested 3.25% (n = 2) were positive to RBT, PAT and TAT and 4.84% (n = 3) were positive MET. In case of 300 goats, 1.67% (n = 5) were positive to RBT and PAT, 2% (n = 6) were positive to TAT and 2.33% (n = 7) were positive to MET. This investigation is the first of its type to be performed in small ruminants in Bangladesh. Higher prevalence rate (8.0 %) was found in BAU nutrition farm in case of sheep and 10 % in Bangladesh Agricultural University (BAU) Veterinary Clinic in case of goat while lower prevalence (0.0 %) was recorded in Pharmacology project and BAU adjacent villages in case of sheep and (0.0 %) in Dhamrai upazila in case of goats respectively. Brucella antibodies were more prevalent in sheep (8.84 %) than in goat (2.33 %).