• BMB Reports
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• 제37권5호
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• pp.538-545
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• 2004

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

• 한국치위생학회지
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• 제22권5호
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• pp.355-363
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• 2022

Objectives: This study aimed to assess the relationship between oral symptoms and changes in daily life after coronavirus disease (COVID-19) in middle and high school students. Methods: Data were obtained from the 17th Korea Youth Risk Behavior Survey (2021). The subjects comprised 53,868 people who responded to survey questions related to this study. Multiple logistic regression analysis with complex samples was performed to identify the correlation between daily life changes and oral symptoms. Results: The analysis showed that compared to the group without changes in daily life after COVID-19, changes in daily life, such as skipping breakfast, drinking, and smoking, either decreased or increased. Furthermore, increase in depression and decreased physical activity and family economic status were related to oral symptoms. Conclusions: It is necessary to develop oral health programs for adolescents suitable for changing situations after COVID-19.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1493-1501
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• 2015

A Bacillus species, EMD4, with strong antibacterial activity was isolated from ganjang (soy sauce) and identified as B. subtilis. B. subtilis EMD4 strongly inhibited the growth of B. cereus ATCC14579 and B. thuringiensis ATCC33679. The antibacterial activity was stable at pH 3-9 but inactive at pH 10 and above. The activity was fully retained after 15 min at 80℃ but reduced by 50% after 15 min at 90℃. The activity was completely destroyed by proteinase K and protease treatment, indicating its proteinaceous nature. The bacteriocin (BacEMD4) was partially purified from culture supernatant by ammonium sulfate precipitation, and Q-Sepharose and Sephadex G-50 column chromatographies. The specific activity was increased from 769.2 AU/mg protein to 8,347.8 AU/mg protein and the final yield was 12.6%. The size of BacEMD4 was determined to be 3.5 kDa by Tricine SDS-PAGE. The N-terminal amino acid sequence was similar with that of Subtilosin A. Nucleotide sequencing of the cloned gene confirmed that BacEMD4 was Subtilosin A. BacEMD4 showed bactericidal activity against B. cereus ATCC14579.

• 한국방사선학회논문지
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• 제17권3호
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• pp.393-399
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• 2023

본 연구에서는 대학생의 가정환경 및 학습태도, 학교생활, 좌석위치가 학업성적에 미치는 영향을 알아보았다. 대전 소재 D대학교의 OO학과 121명 학생을 대상으로 가정환경, 학습태도 및 학교생활에 대해 설문조사를 실시하였고, 성적을 확인하였다. 모든 참여자로부터 동의서를 받았고, 기관연구심의위원회 승인 후 수행하였다. 참여자의 평균 나이는 21.7세였고, 성별은 남자 54.5% 여자 45.5%로 남자가 많았다. 학교생활과 학습태도는 성적에 의미 있는 양의 상관성을 보였고(p=0.001), 가정환경에 대해서는 학교생활(r=0.486, p<0.001)와 학습태도(r=0.454, p<0.001)가 의미 있는 양의 상관성을 보였다. 학교생활과 학습태도 간에도 의미있는 양의 상관성(r=0.437, p<0.001)을 보였다. 좌석위치에서는 남자 보다 여자가 유의하게 높게 나타났고(3.40 vs. 3.89, p=0.004) 학년 간에 통계학적인 유의한 차이가 있었다(p<0.001). 성적 90%이상그룹과 90%미만 그룹 간에 학교생활(4.35 vs. 4.11, p=0.045), 학습태도(3.75 vs. 3.37, p=0.004), 좌석위치(3.89 vs. 3.45, p=0.007) 에서는 통계학적인 유의한 차이를 보였다. 이상의 결과로부터 대학생은 학교생활와 학습태도, 좌석위치는 학업성적에 의미 있는 영향을 미치는 것으로 나타났다.

• 교육녹색환경연구
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• 제11권1호
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• pp.32-41
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• 2012

According to the educational statistics and information data, there were 11,317 elementary school, middle school and high school facility as of 2011. During the post decade, 1,256 schools are increased. The school facility maintenance is the important factor for the whole facility life cycle because it determines the useful life of a facility. Current school facility maintenance practices tend to ad-hok kery, namely, repairment is made after a problem is discovered. The purpose of this study is to propose the new maintenance works and management system to improve the process of maintenance. In addition, the authors suggested school facility improvement plan based upon the questionnaire survey of related management officials.

• 한국학교보건학회지
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• 제36권3호
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• pp.103-112
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• 2023

Purpose: The COVID-19 pandemic, which emerged in late 2019, had a profound impact on global public health and disrupted the daily lives of people worldwide. Particularly, university students faced a challenging situation as their university life underwent a drastic transformation due to long-term remote learning and isolation measures. This study aimed to investigate the relationship between changes in daily life during the 2020 COVID-19 pandemic and depressive symptoms among university students aged between 19 and 29 in Korea. Methods: We analyzed data from the nationally representative 2020 Community Health Survey (CHS). Among the 229,269 participants, 9,279 university students aged 19-29, either enrolled or on leave, were selected. After excluding 401 cases with missing values, the final sample comprised 8,878 individuals. Using multivariate logistic regression with a complex sample design, we explored the association between daily life changes during the COVID-19 pandemic and depressive symptoms. Results: Changes in daily life during the COVID-19 pandemic was associated with depressive symptoms in Korean university students aged 19 to 29, even after adjusting for sociodemographic characteristics, health-related factors, and COVID-19-related aspects (OR=1.28, 95% CI=1.09~1.50). Conclusion: Our study suggests that when examining the impact of COVID-19 on health issues, it is crucial to consider the changes in daily life caused by the pandemic. These findings can provide insights into the psychological well-being of university students during times of crisis.

• 한국치위생학회지
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• 제6권1호
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• pp.63-77
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• 2006

The purpose of this study was to prepare a student and education guideline by examining the college life satisfaction of students studying dental hygiene. Subjects were students going to dental hygiene-related department of three colleges in the Gwangju and Jeonnam areas. College life and life satisfaction was examined at general characteristics. The following results were obtained. 1. Subjects included 35.7% of 1st grade, 33.4% of 2nd grade, and 30.9% of 3rd grade. In the health state, average accounted for the highest percent(76.9%); in the student's associates, a little for the highest percent(76.8%); and in the participation in clubs, no participation for the highest percent(77.1%). 2. For motives to select the dental hygiene, good employment after graduation accounted for the highest percent(61.0%). The highest desired employer was a dental clinic(33.5%), and the highest desired duration was a whole life(61.0%). 3. According to the grade, satisfaction was significantly different in social recognition, major in education, professor, facilities, professor-student, career, and college development(pE0.05). 4. According to the school record, satisfaction was significantly different in social recognition, major in education, professor, professor-student, career, college development, and general life(pE0.01). 5. According to the student's associates, satisfaction was significantly different in social recognition, major in education, professor-student, and general life(pE0.01). 6. According to the participation in clubs, satisfaction was significantly different in professor, facilities, college development, and religious life(pE0.05). In conclusion, as students were in the lower grade, made good school record, had happier associates, and participated in clubs more actively, their satisfaction at college life became higher. Accordingly, professors and school authorities should strive to examine students' actual problem and discuss and resolve them in order to help their effective school life.

• BMB Reports
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• 제39권3호
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• pp.297-303
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• 2006

As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.

• 한국조리학회지
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• 제23권3호
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• pp.242-256
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• 2017

This study was conducted for the university students who majored in culinary arts. The effects of students' certifications on class and school life satisfaction and their effects on preparation employment were examined. The results showed that the students with the certifications had a high level of satisfaction in the class satisfaction, but they also had high stress in the school life. In other words, it seemed that the stress was increased in parallel with the school life in obtaining the certificate. In the case of qualification for preparation employment, the certification affects the preparation for employment even for the culinary arts majors. As for the number of certificates and passions, the change was found from 3 certifications, lower in 4, but again in 5. These results suggest that the certification initially increases enthusiasm, but when it reaches a certain level, it reaches its limit and it rises again after passing the interval. Also, students who graduated from cooking related high school showed high passion compared to those who did not.

• BMB Reports
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• 제50권1호
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• pp.20-24
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• 2017

Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from virus invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNA:tracrRNA:Cas9 complex guides RNase III to cleave target DNA. After cleavage of DNA by CRISPR-Cas9, DNA can be fixed by Non-Homologous End Joining (NHEJ) and Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much more complex and accurate. Gene editing by CRISPR is able to be applied to various biological field such as agriculture and treating genetic diseases in human.