• Title/Summary/Keyword: after parturition

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Differentiation of Thyroid Gland and Changes of Thyroid Hormone Concentration during Early Development of Black Rockfish, Sebastes schlegeli (조피볼락, Sebastes schlegeli 자치어의 갑상선 분화 및 갑상선호르몬의 변화)

  • Kang, Duck-Young;Chung, Ee-Yung;Chang, Young-Jin
    • Development and Reproduction
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    • v.5 no.1
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    • pp.39-45
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    • 2001
  • Black rockfish Sebastes schlegeli is one of scopaenid fish giving birth to yolk-sac larvae and distributed around the coast of Korea and Japan, and is one of main species which is cultured in Korea. The difffrentiation of thyroid gland and the changes of thyroid hormones (THs) concentrations in the whole body during early development of this species were examined and the relationship between thyroid gland and their growth will be presented. Total length (TL) and body weight (BW) of the larva at the parturition were 6.3${\pm}$0.1 mm and 3.6${\pm}$0.1 mg, respectively. The larvae transformed to juvenile after 30th day after parturition. According to Hoshiai(1977), they had gown into stage Fl (TL 13.2${\pm}$0.9 mm, BW 46.5${\pm}$1.5 mg) at 50th day. The thyroid gland of black rockfish was first observed histologically in hatching larvae in mother fish. The larvae just after parturition have 1${\sim}$3 of the thyroid follicles diffrentiated between basibranchial bone and ventral aorta, at the base of the first gill arch. In this time, thyroid follic1e number (TFN),thyroid follicle diameter (TFD) and thyroid cell height (TCH) were 1.6${\pm}$0.8 pieces/inds., 18.1${\pm}$0.6 ${\mu}$m and 4.1${\pm}$0.2 ${\mu}$m, respectively. TFN and TFD at 50th day were increased to 32.5${\pm}$6.9 pieces/inds. and 41.5${\pm}$1.7 ${\mu}$m, respectively. These results indicate that they are related to the growth of black rockfish during early development. However, TCH indicates that the activity of thyroid gland appearedat special day, eg. 5, 20 and 50th day, suggesting that TCH may have some role in the physiological activity. L-thyroxine (T$_4$)concentration decreased sharply to 10 days after parturition, and at 25th day (metamorphosis stage) increased markedly to 3.44${\pm}$0.93ng/g fish. After this time, T$_4$ concentration decreased at 35th day, but then increasedagain to the highest concentration, 5.63${\pm}$0.70ng/g fish. 3,5,3'-triiodo-L-thyronine (T$_3$) concentration declined sharply from just after pafurition (4.96${\pm}$1.90 ng/g fish) to 5th day (0.30${\pm}$0.07 ng/g fish). However T$_3$ concentration increased markedly to 0.95${\pm}$0.11 ng/g fish at 30th day and then did not significantly change until 45th day, increased also sharply to 1.67${\pm}$0.23 ng/g fish at 50th day.

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Study on the Optimum Age of Physiological Reproduction in Korean Native Cattle (한우의 생리적인 최적 번식적령기에 관한 연구)

  • 성환후;이연근;최선호;장원경;이장형
    • Korean Journal of Animal Reproduction
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    • v.26 no.2
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    • pp.193-199
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    • 2002
  • This study was conducted to investigate the optimal physiologic mating time in Hanwoo for protection to decrease of reproductivity and improvement of production of offspring. We observed 32 cows that were devide into 4 parts of treatment : T1(12 months of age and 0.5kg daily gain), T2(12 months of age and 0.8kg daily gain), T3(15 months of age and 0.5kg daily gain) and T4(18 months and 0.5kg daily gain). The first heat of treated cows was 263.3$\pm$6.4 days and average weight was 181.1$\pm$11.3kg. It was revealed the conception rates of first insemination were 25%(T1), 75%(T4) and number of insemination of T3 and T4(both 1.5) was lower than T1 and T2(2.3 and 2.4). In return of estrus after heifer's first parturition, they(T1, T2, T3 and T4) showed 66.2 days, 76.7 days, 62.4 days and 68.5 days respectively and the average was 65.7 days. Plasma progesterone(P4) concentration was nearly the same during the observation periods of treated cows and P4 was released just after 12 months. Only 5 cows (15.6%) in 32 were showed normal estrus cycle and ovulation before 12 months. Before and after parturition, P4 concentration was decreased fastly and then there was no detection of P4 from after parturition to 40 days after milking. P4 would be released again on 45 day after parturition. The results were summarized as that the optimal mating time of Hanwoo heifers was decided by the 14 months of age, 110 cm height and 265kg weight.

Growth Curve of the Gestational Structures by Ultrasonography Examination in Small Pet Dogs (소형 애완견에서 초음파 검사에 의한 임신 구조물의 성장 곡선)

  • Oh, Ki-Seok;Kim, Bang-Sil;Park, Sang-Guk;Park, Chul-Ho;Kim, Jae-Hong;Mun, Byeong-Gwon;Kim, Hee-Su;Lee, Ju-Hwan;Park, In-Chul;Kim, Jong-Taek;Suh, Guk-Hyun;Son, Chang-Ho
    • Journal of Embryo Transfer
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    • v.23 no.3
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    • pp.147-153
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    • 2008
  • Serial ultrasonographic examinations were daily performed from 15 days after ovulation until parturition to determine the growth curve of gestational structures in pregnant Maltese, Yorkshire terrier, Shih-tzu, and Miniature Schnauzer bitches, respectively. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0 ng/ml. The inner chorionic cavity diameter were significantly and linearly relative to gestational age especially days 20 to 40, and the fetal head diameter were significantly and linearly relative to gestational age especial1y day 40 to parturition. These results indicate that inner chorionic cavity diameter were the most accurate for estimating gestational age before day 38 of gestation and the fetal head diameter were after day 38 of gestation.

A quantitative study of the transfer of colostral immunoglobulins to the newborn Korean native calf (한우 송아지의 초유섭취에 의한 수동면역 획득 상태)

  • Kim, Doo;Han, Hong-ryul
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.75-81
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    • 1989
  • The levels of the serum and colostral total protein and immunoglobulins of the Korean native cows immediately after parturition and colostrum-conferred passive immune status of the Korean native calves were studied, and the results obtained were summerized as follows: The mean total protein, total immunoglobulin, IgG, IgM, and IgA concentrations in sera of 10 Korean native cows immediately after parturition were $6.8{\pm}0.4g/dl$, $24.39{\pm}3.41mg/ml$, $19.49{\pm}2.70mg/ml$, $4.56{\pm}1.14mh/ml$, and $0.35{\pm}0.08mg/ml$ respectively. And the mean total protein, total immunoglobulin, IgG, IgM, and IgA concentrations in colostrum were $15.0{\pm}1.1g/dl$, $116.13{\pm}23.07mg/ml$, $101.51{\pm}22.59mg/ml$, $9.46{\pm}1.99mg/ml$, and $5.17{\pm}1.59mg/ml$ respectively. The mean concentrations of total protein and immunoglobulins in colostrum were 2.1 times to 14.8 times higher than those in serum. The mean concentrations of total protein, total immunoglobuln, IgG, IgM, and IgA in sera from 211 Korean native calves at 2 days old were $6.7{\pm}0.9g/dl$, $35.74{\pm}9.56mg/ml$, $29.06{\pm}8.07mg/ml$, $4.91{\pm}2.99mg/ml$, and $1,9{\pm}0.99mg/ml$ respectively. Although the calves had been suckled the dam's colostrum, each of calves was considerably various in levels of serum total protein and immunoglobulins and 16.1 percent of the calves were in a state of hypoglobulinemia.

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Changes of the Osmolarity in Embryonic Body Fluid and the Maternal Ovarian Tissue of the Viviparous Teleost, Ditrema temmincki, during the Gestation Period (태생 경골어류, 망상어(Ditrema temmincki)의 임신기 동안 체내자어의 체액삼투압 및 모체 난소조직상의 변화)

  • LEE Jung Sick;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.2
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    • pp.157-164
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    • 1996
  • Dry weight and body fluid osmolarity of embryo, and maternal ovarian fluid and the ovarian inner tissue of the viviparous teleost, Ditrema temmincki, changed considerably during the gestation period. After the complete absorption of the egg yolk, average dry weight of the embryo increased to 373.76 mg, and the range of total length (TL) was from 6.0 to 60.0 mm. Osmolarity of the embryonic body fluid was 796,8 mOsmol/kg with TL 64.0 mm right before parturition. Ovarian outer membrane started to swelling clearly after fertilization, and maximized in March. The swelling of ovigerous folds was maximized in late April. Expansion of blood vessels and increase of hemocytes reached to their maximum right before parturition. The results of this study indicated that these changes are related to the nutritional and environmental adaptation of both the embryo and the maternal body during the gestation period.

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A Histopathological Observation on the Mammary Gland of Lactating Rat Injected with Dexamethasone (Dexamethasone을 투여(投與)한 Rat 유선조직(乳腺組織)의 병리조직학적(病理組織學的) 관찰(觀察))

  • Ryu, Si-Yun;Lee, Cha-Soo
    • Korean Journal of Veterinary Research
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    • v.23 no.1
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    • pp.57-67
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    • 1983
  • The present study was carried out to observe the histopathological changes in the mammary gland of lactating rats and rabbits injected with dexamethasone. White rats were intramuscularly injected with 0.25mg, 0.5mg or 1.0mg of dexamethasone sodium phosphate (containing $9{\alpha}$-fluoro-$16{\alpha}$-methylprednisolone, 5.0mg/ml) daily for 3 to 10 days on the 3rd day after parturition and white rabbits were intramammary infused with 4mg or 20mg of dexamethasone daily for 4 days on 7th day after parturition. The histopathological changes of the mammary glands, ovaries and adrenal glands of rats and rabbits were observed with light microscope. In the mammary glands of rats, the microscopic findings encountered were decrease of the milk in the alveolar lumina, necrosis and desquamation of epithelial cells, atrophy of alveoli, proliferation of fibroblasts and thickness of alveolar walls, destruction of alveoli, presence of fat droplets within the glandular epithelial cells, infiltration of mononuclear cells and proliferation of adipose tissue, which were relative to the dose and duration of injection. Especially, in the cases of the administration of large doses or long duration, there were severe fibrosis and focal necrosis of glandular tissue. In the mammary glands of rabbits, the morphological changes were similar to those findings in the rats. The milk in the alveolar lumina was decreased gradually according to the dose and duration of injection, while milk fat concentration regarded to increase. In the histological findings of ovaries, necrosis of granulosa cellos, vacuolization and necrosis of luteal cells, atrophy and necrotic foci in the corpora lutes were observed. In the adrenal glands, hyperemia, hemorrhage, vacuolization of adrenal cortical cells, necrotic foci and atrophy of adrenal cortex were observed.

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2-DE and MALDI-TOF MS-based identification of bovine whey proteins in milk collected soon after parturition

  • Lee, Jae Eun;Lin, Tao;Kang, Jung Won;Shin, Hyun Young;Lee, Joo Bin;Jin, Dong Il
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.635-643
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    • 2018
  • Bovine milk is widely consumed by humans and is a primary ingredient of dairy foods. Proteomic approaches have the potential to elucidate complex milk proteins and have been used to study milk of various species. Here, we performed a proteomic analysis using 2-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF MS) to identify whey proteins in bovine milk obtained soon after parturition (bovine early milk). The major casein proteins were removed, and the whey proteins were analyzed with 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE). The whey proteins (2 mg) were separated by pI and molecular weight across pH ranges of 3.0 - 10.0 and 4.0 - 7.0. The 2-DE gels held about 300 to 700 detectable protein spots. We randomly picked 12 and nine spots that were consistently expressed in the pH 3.0 - 10.0 and pH 4.0 - 7.0 ranges, respectively. Following MALDI-TOF MS analysis, the 21 randomly selected proteins included proteins known to be present in bovine milk, such as albumin, lactoferrin, serum albumin precursor, T cell receptor, polymeric immunoglobulin receptor, pancreatic trypsin inhibitor, aldehyde oxidase and microglobulin. These proteins have major functions in immune responses, metabolism and protein binding. In summary, we herein identified both known and novel whey proteins present in bovine early milk, and our sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed their expression pattern.

Predicted functional analysis of rumen microbiota suggested the underlying mechanisms of the postpartum subacute ruminal acidosis in Holstein cows

  • Yoshiyuki Tsuchiya;Ena Chiba;Atsushi Kimura;Kenji Kawashima;Toshiya Hasunuma;Shiro Kushibiki;Yo-Han Kim;Shigeru Sato
    • Journal of Veterinary Science
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    • v.24 no.2
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    • pp.27.1-27.15
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    • 2023
  • Background: The relationships between the postpartum subacute ruminal acidosis (SARA) occurrence and predicted bacterial functions during the periparturient period are still not clear in Holstein cows. Objectives: The present study was performed to investigate the alterations of rumen fermentation, bacterial community structure, and predicted bacterial functional pathways in Holstein cows. Methods: Holstein cows were divided into the SARA (n = 6) or non-SARA (n = 4) groups, depending on whether they developed SARA during the first 2 weeks after parturition. Reticulo-ruminal pH was measured continuously during the study period. Reticulo-ruminal fluid samples were collected 3 weeks prepartum, and 2 and 6 weeks postpartum, and blood samples were collected 3 weeks before, 0, 2, 4 and 6 weeks postpartum. Results: The postpartum decline in 7-day mean reticulo-ruminal pH was more severe and longer-lasting in the SARA group compared with the non-SARA group. Changes in predicted functional pathways were identified in the SARA group. A significant upregulation of pathway "PWY-6383" associated with Mycobacteriaceae species was identified at 3 weeks after parturition in the SARA group. Significantly identified pathways involved in denitrification (DENITRIFICATION-PWY and PWY-7084), detoxification of reactive oxygen and nitrogen species (PWY1G-0), and starch degradation (PWY-622) in the SARA group were downregulated. Conclusions: The postpartum SARA occurrence is likely related to the predicted functions of rumen bacterial community rather than the alterations of rumen fermentation or fluid bacterial community structure. Therefore, our result suggests the underlying mechanisms, namely functional adaptation of bacterial community, causing postpartum SARA in Holstein cows during the periparturient period.

Growth Characteristics of Cultured Dark-Banded Rockfish, Sebastes inermis Cuvier (양식산 볼락, Sebastes inermis Cuvier의 성장 특성)

  • Choi, Hee-Jung;Hong, Kyung-Pyo;Oh, Sung-Yong;Noh, Choong-Hwan;Park, Yong-Ju;Myoung, Jung-Goo;Kim, Jong-Man;Hur, Jun-Wook;Zhang, Chang-Ik;Park, In-Seok
    • Journal of Aquaculture
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    • v.18 no.3
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    • pp.147-153
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    • 2005
  • The objective of this study is to elucidate the growth pattern of dark-banded rockfish, Sebastes inermis Cuvier, produced by aquaculture in Korea. Larvae from 250 maternal full-sibs were reared until 1,350 days after parturition. During the rearing period, growth equations on days after parturition (X) to the total length (TL) and body weight (BW) were estimated as TL=0.0217X $(r^2=0.8867)$ and $BW=0.0001X^2+0.0713X\;(r^2=0.9858)$, respectively. The relationship between BW and TL was $BW=0.0072TL^{3.4373}\;(r^2=0.9945)$. An increases in the eye diameter (ED) of dark-banded rockfish larvae on days after parturition (X) could be expressed as $ED=-2E-5X^2+0.0326X\;(r^2=0.9886)$. Relative growth equations on TL to head length (HL), snout length (SL), ED and postorbital head length (PHL) were calculated as $HL=0.2652TL\;(r^2=0.9758),\;SL=0.0613TL\;(r^2=0.9602),\;ED=0.0752TL\;(r^2=0.9715)$ and $PHL=0.2652TL\;(r^2=0.9501)$, respectively. Since there were few reports on the long-term growth experiment of this species due to difficulties in rearing techniques, results of this study would give a useful information to the biology and commercial culture techniques of dark-banded rockfish.

Study on the Physiology of Optimal reproductive age in Korean Native Cattle (한우의 최적 번식적령기의 생리적 현상에 관한 연구)

  • Seong, H.H.;Lee, J.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.4 no.1
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    • pp.110-118
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    • 2002
  • This study was conducted to investigate the physiology of optimal reproductive age in Hanwoo for protection to decrease of reproductivity and improvement of production of offspring. Thirty two cows were devided into 4 groups of treatments : T1(12 months of age and 0.5kg daily gain), T2(12 months of age and 0.8kg daily gain), T3(15 months of age and 0.5kg daily gain) and T4(18 months of age and 0.5kg daily gain). The days of the first heat of treated cows were 263.3±6.4 days and average weight was 181.1±11.3kg. The conception rates of first insemination were 25%(T1) and 75%(T4), and the number of insemination of T3 and T4(both 1.5) was lower than those of T1 and T2(2.3 and 2.4), respectively. With regard to estrus return after the first parturition, T1, T2, T3 and T4 showed 66.2,76.7, 62.4 and 68.5 days, respectively, indicating the average days of estrus return was 65.7. Plasma progesterone(P4) concentration was nearly the same during the observation periods of treated cows and P4 began to be detected after 12months. Only 5(15.6%) out of 32cows showed normal estrus cycle and ovulation before 12 months. During the peri-parturition period, P4 concentration was rapidly decreased and there was no detection of P4 from parturition to 40 days after milking. P4 would be released again on 45 day after parturition. The results imply that the optimal reproductive age of Hanwoo heifers would be around at the 14 months of age, 110cm height and 265kg weight.