• Natural Product Sciences
• /
• 제4권2호
• /
• pp.100-104
• /
• 1998

The antimutagenic activity of a methanol extract of roasted Cassia tora seed against aflatoxin $B_1(AFB_1)$ was demonstrated with the Salmonella typhimurium assay. The numbers of revertants per plate decreased significantly when this extract was added to the assay system using Salmonella typhimurium TA100 and or TA98. The MeOH extract was then sequentially partitioned with $CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$. The $CH_2Cl_2$ and n-BuOH fractions possessed antimutagenic activity, but the EtOAc and $H_2O$ fractions were inactive. Both the MeOH extract and its fractions were capable of inhibiting the indirect-acting mutagen $AFB_1$, suggesting that these fractions may prevent the metabolic activation of $AFB_1$, or scavenge the electrophilic intermediate capable of inducing mutations. Column chromatography using silica gel yielded pure alaternin from the $CH_2Cl_2$ a fraction, and adenosine and isorubrofusarin gentiobioside from the n-BuOH fraction. Alaternin and isorubrofusarin gentiobioside demonstrated significant antimutagenic activities.

• 한국식품영양과학회지
• /
• 제31권6호
• /
• pp.986-993
• /
• 2002

항암성과 향미가 개선된 기능성 재래식 버섯균사체메주는 버섯균을 액체배양하여 재래식메주의 각 면에 5개씩 만든 구멍(1X3 cm)에 버섯균배양액을 메주 무게의 10%를 접종한후 $25^{\circ}C$에서 4주간 배양하여 제조하였다. 제조한 버섯균사체 메주 중 상황, 영지, 또는 신령버섯균사체메주가 항암성과 향미완화능이 우수하였다.

• Preventive Nutrition and Food Science
• /
• 제4권1호
• /
• pp.33-37
• /
• 1999

The antimutagenic effects of buchu kimchi and Chinese cabbage kimchi and theri cytotoxic effects against human cancer cell line were investigated in the Salmonella typhimurium system and MTT assay, respectively. Leek and Chinese cabbage were aslo evaluated in the same system. Buchu kimchi was fermented at 15 $^{\circ}C$ for 4 days . Buchu kimchi samples showed somewhat higher antimutagenic effects against aflatoxin B1(AFB1) than CHinese cabbage kimchi in Salmonella typhimurium TA100 strain. There was no difference onthe antimutagenic activity according to the length of fermentation . Leek exerted stronger antimutagenicity against AFB1 than Chinese cabbage in the Ames assay. In MTT assay, 6-day fermented buchu kimchin revealed the highest cytotoxicity against AGS human gastric adenocarcinoma cells in which 62% and 82% of the inhibition were observed wiht the addition of 100ug, 400ug/well, respectively. Buchu kimchi samples caused 60~70% inhibition on the proliferation of HT-29 at 400ug/well. Leek exhibited higher antiproliferative effect against both AGS cells and HT-29 cells than Chinese cabbage in MTT assay. From these results, it is considered that buchu kimchi has stronger antimutagenic and in vitro anticancer effects than Chinese cabbage kimchi and the high inhibition rate of buchu kimchi probably results from leek, the major ingredient of buchu kimchi .

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
• /
• pp.174-174
• /
• 1993

목적: Cinnamic acid의 유도체들중에서 항돌연변이 효과가 보고된 바 있어, 본 연구에서는 E. coil PQ37균주를 사용한 SOS chromotest를 이용하여 약 170개 cinnamic acid의 유도체들의 항돌연변이 효과를 확인하고자 하였고, 1차로 확인된 수종의 유도체들에 대한 결과를 보고하고자 한다. 방법: SOS chromotest는 sul A:: lacz fusion strain 인 E. coli PQ37 에서의 chemical로 인한 DNA damage에 대한 SOS response를 $\beta$-galactosidase enzyme activity level로서 측정하는 실험이며, m-RNA 또는 protein synthesis에 대한 test chemicals의 cytotoxic저해효과를 알아보기 위하여 alkaline phosphatase를 병행측정하여 보완하였다. 결과 및 고찰: (－)S－9 경우는 4-nitroquinoline 1-oxide(4-NQO)를 유도물질로 하였으며 대략 Induction factor가 9.7 정도였고, (＋)S－9의 경우는 aflatoxinB$_1$을 유도물질로 하였고 이때의 Induction factor는 13.8 정도였다. 이결과 1차로 test된 cinnamic acid유도체 6개중에서 RK001, RK002, RK003, RK004, RK005는 거의 항돌연변이 효과를 나타내지 않았으나, RK006은 항돌연변이 효과를 보여주어 이들의 Structure-Activity Relationship (SAR) 및 Dose-response와 RK006의 항돌연변이 효과의 mechanism에 관해 M13 mp2 viral DNA의 유전자 염기서열을 이용하여 항돌연변이 기전연구를 수행중이다.

• 한국식품영양과학회지
• /
• 제27권2호
• /
• pp.350-358
• /
• 1998

The antimutagenic and anticancer activities of glycoprotein(GP) and chondroitin sulfate(CS) from sea cucumbers were studied using Ames mutagenicity test and human cancer cells culture test. The GP's inhibitory effect toward aflatoxin B1(AFB1) and 3, 2'-dimethyl-4-amino-biphenyl(DMAB) increased with the higher added concentrations up to 5% level(w/w) regardless fractionation methods. The GP from sea cucumbers through DEAE-cellulose column chromatography showed an inhibitory effect ranged from 84 to 98%, and the maximum antimutagenicities resulted in red sea cucumber with 98% (AFB1) and 95% (DMAB). But 5% level of CS from various sea cucumbers had an inhibitory effect toward those both indirect mutagens ranged from 79% to 85%. However, in case of direct mutagens(N-methyl-N'-nitro-N-nitrosoguanidine, MNNG and 4-nitroquinoline-1-oxide, 4-NQO), the GP's inhibitory effect was 55∼78% and the CS had a low inhibitory effect(58∼70%) at the added level of 5%. The GP from sea cucumbers exhibited the strong inhibitory effects with 89∼95% and 82∼92% on the growth of HT-29 human crcinoma cells and AZ-521 human gastric cancer cells (at 5% level).

• 한국식품영양과학회지
• /
• 제23권3호
• /
• pp.436-442
• /
• 1994

The effect of garlic on glutathione S-transferase activity and the level of glutathione in the mouse liver was studied. the intraperitoneal injection of the methanol extract of garlic and ally sulfide which is one of possible active compounds in garlic to ICR mouse before the injection of aflatoxin B1 (AFB1) increased the levels of glutathione and nonprotein-SH in microsomal fraction of the livers. The injection of the chloroform fraction 2 which revealed the highest antimutgenic activity in our previous research in the increase of the activity of glutathione S-transferase and the levels of glutathione and nonprotein -SH. The glutathione itself also had the antimutagenic effect on AFB1 and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA98 and TA100 in vitro.

• 한국의류학회지
• /
• 제24권8호
• /
• pp.1146-1154
• /
• 2000

The purpose of this study was to investigate the antimicrobial activity, antimutagenic and anticancer effects and dyeing properties of the fermented indigo extract. The methanol extract of fermented indigo showed a strong inhibition effect on Trich. mentagrophytes and antimutagenic activities against aflatoxin B$_1$(AFB$_1$) in the Ames test using Salmonella typhimurium TA 100. We also found in vitro anticancer effects of the methanol extract of fermented indigo and it was evaluated by using Clone M-3 mouse melanoma cells and A431 human epidermoid carcinoma cells and exerted little cytotoxity against 3T3-L1 embryo fibroblast cell. In the relationship between the K/S values of cotton and silk fabrics dyed with fermented indigo and dyeing repeating times, the K/S values became higher as the repeating times were increased. The K/S values were high when the fabrics were dyed at low temperture. The K/S values of cotton fabrics were higher than those of silk fabrics. Changes of surface color of silk fabrics were higher than those of cotton fabrics after water fastness test, laundering, irradiation and treatment of acidic and alkaline perspiration.

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2003년도 춘계학술대회 논문집
• /
• pp.59-59
• /
• 2003

Aflatoxins are produced by Aspergillus flavus, parasiticus and their related fungi that grow in improperly stored foods such as com, rice, peanuts and other cereals. In addition to its high mutagenicity and cytotoxicity, aflatoxin B$_1$ (AFB$_1$) is a potent hepatocarcinogen in experimental animals and an important factor for the human liver cancer. In spite of a high attention to the hepatocarcinogenicity of aflatoxins, the relative toxicity, for the risk assessment, of other types (AFB$_2$, AFG$_1$ and AFG$_2$) of the toxin was not fully studies.(omitted)

• 한국식품저장유통학회지
• /
• 제8권4호
• /
• pp.399-404
• /
• 2001

김치 첨가 해물만두의 생리기능성을 조사하고자 시판 만두, 해물만두 및 20% 김치첨가 해물만두 추출물에 대한 항돌연변이, 항산화 효과 및 아질산염 소기능에 대하여 조사하였다. 간접돌면변이원으로 알려진 aflatoxin B$_1$을 첨가한 Salmonella typhimurium TA 100 및 YG 1024에 만두 추출물을 5 mg/plate 농도로 처리한 후 항돌연변이 효과를 조사한 결과 20% 김치첨가 해물만두의 돌연변이 저해율이 47% 및 61%로 가장 높게 나타났으며, 수소공여능은 모두 50% 이상의 활성을 나타내었고, 시판만두, 해물만두 및 20% 김치첨가 해물만두의 순으로 높게 나타났으나, 모두 0.1%의 BHT 첨가군 보다는 낮았다. Linoleic acid에 대한 과산화물가는 저장기간이 지남에 따라 대조군의 과산화물가는 크게 증가한 반면 20% 김치첨가 해물만두 첨가군에서는 대조군에 비하여 매우 낮게 나타났으며, 시판 및 해물만두 추출물의 경우는 약간 낮게 나타났으나 0.1% BHT 첨가군보다는 모두 높았다. 횐쥐의 liver homogenate에 만두추출물을 처리한 후 TBA가를 조사한 결과 20% 김치첨가 해물만두 추출물 처리구가 가장 낮게 나타났다. 아질산염 소거능은 모두 50% 이상이었다.

• 대한환경공학회지
• /
• 제31권1호
• /
• pp.35-41
• /
• 2009

본 연구는 토착미생물에 의한 발효기작을 이용하여 음식물쓰레기를 동물 사료로 이용하기 위한 가능성을 평가하기 위하여 수행되었다. 이를 위하여 효소활성 실험을 통하여 anylase, protease, lipase에 대한 우수활성을 나타내는 토착유효미생물을 토양으로부터 분리하여 음식물쓰레기의 사료화를 위한 발효제로 사용하였다. 16s rDNA sequencing의 분석을 통해 우수활성을 나타내는 미생물(H1, D1)은 Bacillus subtilis와 Paenibacillus polymyxa인 것으로 확인되었다. 사료화 실험에서는 수분과 영양소 함량의 조절을 위하여 쌀겨 및 팽화미를 음식물쓰레기에 혼합하여 사료화를 위한 시료로 사용하였으며 효소활성 실험을 통해 분리된 2종의 미생물(Bacillus subtilis, Paenibacillus polymyxa)을 발효제로 사용하여 사료화 실험을 진행하였다. 발효실험 동안의 온도, pH 및 ORP 측정을 통해 토착유효미생물의 접종에 의한 음식물쓰레기의 발효가 효과적으로 진행됨을 확인할 수 있었다. 반응종료후 제조된 사료의 영양분 분석 결과에서 조단백질, 조지방, 조섬유은 동물 급여에 충분한 함량을 나타내었다. 또한 Pb, Hg, Cd, aflatoxin 및 salmonella 등의 유해물질의 분석결과 모든 항목이 허용기준치 이하의 값을 나타내었다. 따라서 토착유효미생물을 이용한 음식물쓰레기의 사료화는 동물의 사료로써 충분히 활용될 수 있을 것으로 기대된다.