• The Korean Journal of Pharmacology
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• v.17 no.2
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• pp.17-22
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• 1981

In the electrically stimulated guinea-pig ileum, which was incubated in the modified Krebs-Henseleit bicarbonate buffer solution containing various concentrations of electrolytes at $4^{\circ}C$ for 24 hours, the effect of naloxone on the inhibitory action of morphine was investigated. Incubation potentiated the inhibitory action of morphine. In the incubated preperation, the inhibitory action of morphine was potentiated in the $Na^+\;75mM$, and $K^+\;2.9mM$ groups, while that action of morphine was reduced in the $Ca^{++}\;3.6mM,\;Mg^{++}$ free and $Mn{++}\;0.2mM$ groups. Naloxone in incubation media potentiated in the inhibitory action of morphine. In the preparations which were incubated in various concentrations of electrolytes plus naloxone, the action of morphine was reduced in $Na^+\;75mM,\;K^+\;2.9mM$, and $Ca^{++}\;3.6mM$ groups, while that action of morphine was potentiated in $Mg^{++}$free and $Mn{++}\;0.2mM$ groups. Naloxone antagonised those actions of morphine. However, $pA_2$ values for naloxone (index for affinity for antagonist) was not changed. Thus changes in the inhitory action of morphine caused by incubation are probably not the result of changes in the affinity of receptor, but due to the alterations in the events which precede or follow the receptor binding by incubations.

• 한국경영정보학회:학술대회논문집
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• 2007.11a
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• pp.88-95
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• 2007

사회적 커뮤니케이션 미디어는 기업 조직의 사회적 네크워크를 활발히 사용되고 있다. 각 커뮤니케이션 정보시스템은 매우 유용하게 사용되고 있지만, 업무적 특성과 사회적 영향 요인에 대한 연구는 매우 적은 편이다. 또한 새로운 정보기술의 도입, 예를 들면, 메신저와 무선 화상통신과 같은 발전은 매체 풍부성 이론으로 아직까지 적용 검증되지 못하고 있다. 본 연구는 업무적 특성과 미디어 활용 그리고 업무성과에 대한 검증을 실시하였다. 분석방법은 서베이를 통하여 소중대기업에서 근무하고 있는 280명을 대상으로 하였고 회귀분석으로 분석결과를 도출하였다. 연구결과로는 업무 특성은 미디어 사용에 긍정적인 영향요인이며 사회적 영향과 친분관계는 두 관계를 조절하고 있음을 발견하였다. 또한 커뮤니케이션 미디어의 활용은 각 업무성과에 긍정적인 영향을 미치고 있음을 발견하였다.

• Journal of Ocean Engineering and Technology
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• v.20 no.6 s.73
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• pp.35-40
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• 2006

In recent years, there's been strong demand for seawalls that havea gentle slope and permeability that serveswater affinity and disaster prevention from wave attack. The aim of this study is to examine wave transformation, including wave run-up that propagates on the coastal structures. A numerical model based on the weak nonlinear dispersive Boussinesq equation, together with the unsteady nonlinear Darcy law for fluid motion in permeable layer, is developed. The applicability of this numerical model is examined through Deguchi and Moriwaki's hydraulic model test on the permeable slopes. From this study, it is found that the proposed numerical model can predict wave transformation and run-up on the gentle slope with a permeable layer, but can't show accurate results for slopes steeper than about 1:10.

• Bulletin of the Korean Chemical Society
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• v.11 no.6
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• pp.552-557
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• 1990

Luminescence quenching reactions of photoexcited tris(2,2'-bipyridine)ruthenium (Ⅱ) complex cation, $Ru(bpy)_3\;^{2+}$, by dialkylviologens (dimethyl, dioctyl, dibenzyl, methyloctyl, methyldodecyl, and methylbenzyl) were studied in sodium dodecylsulfate (SDS), poly(styrenesulfonate) (PSS), and poly(vinylsulfonate) (PVS) solutions. The relative quenching rate varies widely with the microheterogeneous media employed: the highest quenching rate is observed for methyldodecylviologen in homogeneous aqueous medium, dibenzylviologen in SDS and PVS solutions, and dimethylviologen in PSS solution; the lowest rate is found for dimethylviologen in homogeneous medium and SDS solution, methyldodecylviologen in PSS and PVS solutions. These results were interpreted in terms of reduction potential of viologens, affinity of $Ru(bpy)_3\;^{2+}$ and viologens to the microparticles, and the structures of the viologen-colloid complexes.

• Journal of Broadcast Engineering
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• v.20 no.3
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• pp.368-378
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• 2015

3D conversion technology has been studied over past decades and integrated to commercial 3D displays and 3DTVs. The 3D conversion plays an important role in the augmented functionality of three-dimensional television (3DTV), because it can easily provide 3D contents. Generally, depth cues extracted from a static image is used for generating a depth map followed by DIBR (Depth Image Based Rendering) rendering for producing a stereoscopic image. However except some particular images, the existence of depth cues is rare so that the consistent quality of a depth map cannot be accordingly guaranteed. Therefore, it is imperative to make a 3D conversion method that produces satisfactory and consistent 3D for diverse video contents. From this viewpoint, this paper proposes a novel method with applicability to general types of image. For this, saliency as well as edge is utilized. To generate a depth map, geometric perspective, affinity model and binomic filter are used. In the experiments, the proposed method was performed on 24 video clips with a variety of contents. From a subjective test for 3D perception and visual fatigue, satisfactory and comfortable viewing of 3D contents was validated.

• Microbiology and Biotechnology Letters
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• v.16 no.5
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• pp.335-342
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• 1988

An anion exchange chromatography was employed for the purification of mouse monoclonal antibodies from ascitic fluid and in vitro cultivation media. After cultivation of hybridomas, Alps 25-3, HCGK, A4W, and KW, producing IgG1, the culture supernatants were harvested by centrifugation, precipitated with 50-60％ ammonium sulfate, and dialyzed against 0.025 M Tris-HCI buffer (pH 8.2). Then the dialyzed samples were loaded into a DEAE-Trisacryl M anion exchange column. Monoclonal antibodies bound to the DEAE-Trisacryl M were eluted with 0.025 M Tris-HCI buffer (pH 8.2) containing 30-40 mM NaCl. In ammonium sulfate precipitation, the recovery of the monoclonal antibody was shown to be 90％ and 84％ from in vitro culture media containing 10％ and 2％ fetal bovine serum, respectively. On the other hand, the pretreatment by ultrafiltration enhanced the yield up to 91％ whereas the purity was lower than that by ammonium sulfate treatment. Subsequently, in the DEAE-Trisacryl M chromatographic separation, the purities and recoveries of all the monoclonal antibodies from both the in vitro culture supernatants and ascitic fluids were 70-80％ and 65％ respectively. The monoclonal antibody, Alps 25-3 could be further purified with a purity of 95％ through an immunoadsorbent chromatography.

• The Korean Journal of Pharmacology
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• v.18 no.2
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• pp.33-44
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• 1982

The binding in vitro of an opiate agonist, $(^3H)-morphine$, was studied using rat brain slices which were incubated in the modified Krebs-Henseleit bicarbonate buffer solution containing various concentrations of electrolytes with or without morphine, naloxone or morphine+naloxone at $4^{\circ}C$ for 24 hours. The binding of $(^3H)-morphine$ may be seperated into two component; one a saturable binding and the other nonsaturable. The saturable binding may be calculated from the differences in binding observed in the absence and presence of high concentration of morphine. The maximal saturable binding and $K_D$ value in the naive preparations were $0.32{\pm}0.02\;pmole/mg$ protein and $0.75{\pm}0.07\;nM$ respectively. The saturable binding of $(^3H)-morphine$ was significantly increased by low temperature-treatment, while $K_D$ value was not changed. Morphine in the incubation media significantly increased the saturable binding of $(^3H)-morphine$ and $K_D$ value. Naloxone also increased the maximal saturable binding of $(^3H)-morphine$ and $K_D$ value of the drug. Decrease of $K^+\;and\;Mg^{++}$, and addition of $Mn^{++}$ in the incubation media significantly increased the saturable binding of $(^3H)-morphine$, but decrease of $Na^+$and increase of $Ca^{++}$ in the incubation media did not influence the binding. The increment of the saturable binding of $(^3H)-morphine$ by nonlabeled morphine in the incubation media was notaffected by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or addition of $Mn^{++}$ into the incubation media, but was inhibited by increase of $Ca^{++}$ in the incubation media, while the increment of the saturable binding of $(^3H)-morphine$ was net observed by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or increase of $Ca^{++}$ in the incubation media. The above results indicate that change of opiate binding sites in quality, i.e. affinity, and quantity, i.e. number of binding sites, may occur by low temperature-treatment in the absence and presence of morphine or naloxone and that electrolytes play role of the changes of opiate binding sites.

• Journal of Korean Library and Information Science Society
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• v.53 no.3
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• pp.263-285
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• 2022

This study presented the factor analysis on constructing the new factors affecting the middle school students' online learning behaviors from the questionnaires employed among middle school students. A total of 204 students participated and the data were collected in South Korea. The sample of middle school ninth-grade students was selected and used through purposive sampling. Findings from the factor analysis provided evidence for the eight-factor solution for the 35-items accounting for 66.15% of the shared variance. A wide range of factors has been considered to identify students' online learning behaviors. The appropriate experience and use of e-learning in the middle school period is also important as it will be a critical stepstone for future education. This research provides information that has been taken into account for advancing online learning to enhance the quality of e-learning systems for middle school students. The study results provided eight new factors affecting the middle school students' online learning behaviors; that is 1) communication using social media as a learning tool, 2) intention to share information using ICT, 3) addiction of technology, 4) adoption of technology, 5) seeking information using ICT, 6) use of social media learning, 7) information search using ICT, and 8) immersion of technology. This study confirmed that middle school students prefer communication using social media as a learning tool, and value intention to share information using ICT for the most part. The data obtained based on factor analysis can highlight the online learning behaviors towards a mixture of social media learning and ICT to ensure a new educational platform for the future of e-learning. This research expects to be useful for both middle schools of online learning to better understand students' online learning behaviors and design online learning environments and information professionals to better assist students who particularly need digital literacy.

• Journal of the Korean Chemical Society
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• v.23 no.3
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• pp.141-151
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• 1979

The selective absorption of metal ions by chelating agent-loaded resins was studied in aqueous media. The resins were prepared by loading the conventional anion exchange resin, Dowex 1-X8 (50 to 100 mesh) with chelating agents containing sulfonic group, such as 8-hydroxy-quinoline-5-sulfonic acid (HQS) and 7-nitroso-8-hydroxyquinoline-5-sulfonic acid (NHQS). The stability of the resin was markedly influenced by the following factors; (1) the affinity and concentration of anions in the external solution, (2) the pH of the media. The optimum conditions for the absorption of metal ions were determined with respect to the pH, shaking time, and the effect of anion concentration in the medium. Under the optimum condition the order of the absorption of metal ions such as Fe(Ⅲ), Cu(Ⅱ), Pb(Ⅱ), and Zn(Ⅱ) was in accord with that of the stability constants of the chelates. The total capacities of the resins were found in the range of 0.6∼1.6 mmole metal per gram.

• Journal of the Korean Electrochemical Society
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• v.3 no.2
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• pp.69-71
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• 2000

Redox-active calix[4]arenes with carboxylic acid and disulfide groups were prepared and spontaneous deposition on silver and gold surfaces was observed. Owing to their unusual structure, the calix[4]arenes exhibit selective affinity fur alkaline earth metal ions in aqueous media. When annular ionophores are immobilized on the surface, voltammetric and spectroscopic studies show the entrapment of metal ions. Furthermore, it was possible to reversibly capture and remove the ions using strong chelating agents such as ethylenediaminetetraacetic acid (EDTA).