In this system, rice cells were genetically modified to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter induced by sugar depletion. Even though the target protein fused with signal sequence peptide, plant cell wall can be a barrier against secretion of recombinant proteins. Therefore, hCTLA4Ig can be trapped inside cell wall or remained in intracellular space. In this study, to enhance the secretion of hCTLA4Ig from cytoplasm and cell walls into the medium, permeabilizing agents, such as dimethyl sulfoxide (DMSO), Triton X-100 and Tween 20, were applied in transgenic rice cell cultures. When 0.5% (v/v) of DMSO was added in sugar-free medium, intracellullar hCTLA4Ig was increased, on the other hand, the secreted extracellular hCTLA4Ig was lower than that of control. DMSO did not give permeable effects on transgenic rice cell cultures. And Triton X-100 was toxic to rice cells and also did not give enhancing permeability of cells. When 0.05% (v/v) Tween 20 was added in rice cell cultures, however, intracellular hCTLA4Ig was lower than that of control cultures. And the maximum 44.76 mg/L hCTLA4Ig was produced for 10 days after induction, which was 1.4-fold increase compared to that of control cultures. Especially, Tween 20 at 0.05% (v/v) showed the positive effect on the secretion of hCTLA4Ig though the decrease of intracellular hCTLA4Ig. Also, Tween 20 as a non-toxic surfactant did not affect the cell growth, cell viability and protease activity. In conclusion, secretion of hCTLA4Ig could be increased by enhancing permeability of cells regardless of the cell growth, cell viability and protease activity.
Kim, Jaedong;Seo, Hyunggoog;Cha, Seunghoon;Noh, Junyong
Journal of the Korea Computer Graphics Society
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v.21
no.3
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pp.1-10
/
2015
One way to create a visually immersive environment is to utilize a front projection system. Especially, when enough space is not available behind the screen, it becomes difficult to install a back projection system, making the front projection an appropriate choice. A drawback associated with the front projection is, however, the interference of shadow. The shadow can be cast on the screen when the user is located between the screen and the projector. This shadow can negatively affect the user experience and reduce the sense of immersion by removing important information. There have been various attempts to eliminating shadows cast on the screen by using multiple projectors that compensate for each other with missing information. There is trade-off between calculataion time and desired accuracy in this mutual compensation. Accurate estimation of the shadow usually requires heavy computation while simple approaches suffer from inclusion of non-shadow regions in the result. We propose a novel approach to removing shadows created in the front projection system using the skeleton data obtained from a depth camera. The skeleton data helps accurately extract the shape of the shadow that the user cast without requiring much computation. Our method also utilizes a distance field to remove the afterimage of shadow that may occur when the user moves. We verify the effectiveness of our system by performing various experiments in an interactive environment created by a front projection system.
With the explosive growth of the Internet, Internet shopping malls have become recognized as one of the major purchasing channels for consumers, as well as one of the competitive distribution channels for companies that allow them to contact with customers without intermediaries. It has motivated information systems(IS) researchers to examine the factors influencing consumer behavior and the purchase decision process in the context of Internet shopping malls. Despite the extensive research that has been conducted on the purchase decision process of consumers in online shopping malls, the results have demonstrated a need for further understanding of consumer behavior due to the unique features of virtual space and the characteristics of online consumers. Previous studies from marketing and consumer behavior domains have suggested that the concept of involvement plays an important role in explaining consumers' purchase behavior. Despite the critical role of involvement and the explosive growth of e-commerce, little research has examined the role of involvement in the Internet shopping mall context. With this motivation, this study has two research objectives. First, it introduces and tests an theoretical model capable of better explaining consumers' intention to purchase in the Internet shopping mall context. The proposed model extends and integrates existing models on purchase intention by incorporating purchase experience, innovativeness, and perceived self-control as the consumer factors, along with perceived risk, information provision, and perceived price as the Internet shopping mall factors. Second, this study examines how involvement differences may affect consumers' intention to purchase. For this purpose, two factors from involvement theory, involvement type and involvement level, are introduced into the research model as moderating variables. In order to test the proposed model, the overall approach employed was a field study using the structural equation model. We developed our data collection instrument by adopting existing validated questions wherever possible. All question items were measured with a seven-point, Likert-type scale, with anchors ranging from 'strongly disagree' to 'strongly agree.' Two IS researchers reviewed the instrument and checked its face validity. We collected empirical data for this study over a period of two weeks from subjects who had purchase experiences through Internet shopping malls. A total of 473 complete and valid responses were obtained. We carried out data analysis using a two-step methodology with AMOS 4.0. The first step in the data analysis was to establish the convergent and discriminant validity of the constructs. In the second step, we examined the structural model based on the cleansed measurement model. The empirical results partly support the proposed model and identify the moderating effect of involvement differences. Theoretical and practical implications of the study are discussed, along with its limitations.
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
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pp.186-186
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2017
Relay intercropping system of direct seeded rice and winter cereal is labor saving cultivation and has high land use efficiency in Japan. In this system, rice seeds are direct seeded into inter-row space of winter cereals (wheat or barley) in March or April. If the direct seeding of rice is conducted before stem elongation phase or using suitable seeder, these are little effect to yield of winter cereals. Though the seeds are generally thiuram treated, it's a matter that seedling establishment rate (SER) of direct seeded rice is low and unstable. The cause of low SER has not been revealed. In present study, with the aim to reveal causes of low SER, we conducted experiments and investigated the SER, and analyzed some factors that might affect SER. Experiment1: In 2015, 2016, we buried rice seeds underground, and investigated the transition of the seed survival rates (SSR). Seeds were thiuram-treated or non-treated. In 2 periods, SSR of thiuram-treated seeds were significantly higher than non-treated seeds. In 2016, thiuram-treated seeds were high in SSR (almost 75%) at April 30th, but low SER (10~27%) after harvest of winter cereals. Therefore, almost all of seed death might have been happen after germination. Analysis 1: We investigated the SER and cultivation conditions in Ibaraki pref. for several years. Meteorological factors were referred from the nearest point of AMeDAS. From mean temperature (MT) among 5days after and before the day, we divided the period of seeding ~June 20 to phase1~4. We defined each phase as below; Phase1: $MT{\leq}10^{\circ}C$, Phase2: $10^{\circ}C<MT{\leq}15^{\circ}C$, Phase3:$15^{\circ}C<MT{\leq}20^{\circ}C$, Phase4: $MT>20^{\circ}C$. We analyzed the correlation of SER and meteorological factors by each phase. Total number of days in phase 1~4 was significantly negative correlated with SER. In phase1, total rain fall and number of soil wetting days were significantly negative correlated with SER. In phase2~4, only MT was significantly positive correlated with SER. This result suggested that rainfalls in phase1 declined seed vigor to emergence from soil surface, by repeated water absorption and re-dry. From these present studies, it was suggested that one of factors of low SER of direct seeded rice in relay intercropping system is changing of water condition by rainfalls in phase1 ($MT{\leq}10^{\circ}C$). To improve SER, it's necessary to consider something seed treatments such as prevent water absorption during phase1. However, 58~60% of seeds seemed to die during May. It's suggested that, if seeds are thiuram treated, almost all of seeds can germinate underground, but the seed vigor to emergence from soil surface are insufficient. Further studies are needed to reveal the rest causes that is happening during emergence from soil surface.
Journal of the Korean Society for Library and Information Science
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v.39
no.1
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pp.45-58
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2005
This Paper is a comparative study of feature selection methods for Korean web documents clustering. First, we focused on how the term feature and the co-link of web documents affect clustering performance. We clustered web documents by native term feature, co-link and both, and compared the output results with the originally allocated category. And we selected term features for each category using $X^2$, Information Gain (IG), and Mutual Information (MI) from training documents, and applied these features to other experimental documents. In addition we suggested a new method named Max Feature Selection, which selects terms that have the maximum count for a category in each experimental document, and applied $X^2$ (or MI or IG) values to each term instead of term frequency of documents, and clustered them. In the results, $X^2$ shows a better performance than IG or MI, but the difference appears to be slight. But when we applied the Max Feature Selection Method, the clustering Performance improved notably. Max Feature Selection is a simple but effective means of feature space reduction and shows powerful performance for Korean web document clustering.
Most large cut slopes of open pit mines, roadways, and railways are steeply inclined and composed with rocks that do not contain soils. However, these rock slopes suffer both weathering and fragmentation. In the case of steep slopes, falling rock and collapse of a slope may often occur due to surface erosion. Cast-in place concrete and rubble work are the most widely used earth structure-based pressure supports that act as restraints against the collapse of the rock slope. In order to overcome the shortcomings of conventional retaining walls, a segmental grid retaining wall is being used with connects precasted segments to construct the wall. In this study, laboratory model test was conducted to estimate deformation behavior of segmental grid retaining wall with configuration of rear strecher, height and inclination of the wall. In order to examine the behavior characteristics of a segmental grid retaining wall, this research analyzes the aspects of spacial displacement through relative displacement according to change in the inclination of the wall. Also, the walls behavior according to the formation and status of the rear stretcher which serves the role of transferring the load from the header and the stretcher which make up the wall, the displacement of backfill materials in the wall, and the location of the maximum load were surveyed and the characteristics of displacement in the segmental grid retaining wall were observed. The test results of the segmental grid retaining wall showed that there was a sudden increase in failure load according to the decrease in the wall's height and the size of the in was greatly decreased. Furthermore, it revealed that with identical inclination and height, the structure of the rear stitcher did not greatly affect the starting point or size of maximum horizontal displacement, but rather had a stronger effect on the inclination of the wall.
Kim, Ki-Rim;Song, Je-Seon;Choi, Byung-Jai;Kim, Seung-Hye;Lee, Jae-Ho
The Journal of Korea Assosiation for Disability and Oral Health
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v.7
no.1
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pp.25-28
/
2011
Autism is a developmental, neuropsychiatric disorder that begins in early childhood. A patient with autism seen in the dental office frequently may have many complications. Therefore, it may be needed to consider modified or alternative therapy for dental care of autistic patients. This is the case of a 16-year old boy who have autism. He came to the department of the pediatric dentistry, Yonsei University Dental Hospital, for evaluation and treatment of dentigerous cyst associated with impacted teeth(#33,34,35). Under daily hospitalization and general anesthesia, the cyst was enucleated with surgical extraction of #34 and autotransplantation of #33,35. And during the periodic dental followup, apexification of #33,35 was performed for periapical lesion and root maturity. At 2 year 6 months follow- up, now, bony healing was completed and there are some complications like external resorption of #33 and space loss of #34 area. Generally, the marsupialization has been widely recommended for treatment of dentigerous cyst. However, in this case, there is a little possibility of spontaneous eruption after marsupialization considering of patient's age, location and angulation of the impacted tooth, root maturity. And there is necessity to choose the treatment that has low recurrence risk and needs short-term follow-up for autism. Above all, poor oral hygiene and lack of cooperation for decompression treatment is a matter of primary consideration. Consequently, enucleation of the cyst was chosen for the final treatment plan in this case. It is important to consider the conditions that affect the eruption of a dentigerous cyst-associated tooth to predict the successful eruption and special health care needs of the patient when the treatment plan is settled.
A cyber crime has been evolving, as other crimes have. Beyond physical damages such as financial crimes in the cyber world, it has evolved into psychological forms like cyber-bullying. In today's crimes, measures that consider psychological effects of crimes are highly regarded in that fear of crime is considered as well as visible crimes. Accordingly, cyber bullying, which causes psychological damages, needs to be treated very importantly. Because the characteristics of cyber crime is caused by different circumstances from the real world, measures should be taken in different aspects. Rather than physical damages, which can be found in the conventional crimes, cyber crimes cause psychological damages, so the effect that has on inflicting should be specified. Especially, in the environment that virtual reality is ubiquitous through smart phones, it is very difficult to establish measures by considering all the causes of general cyber crimes. Therefore, this study estimated what affects cyber bullying by focusing on personalized faith factor among social bond theory, which can control cyber crimes. As a result, self-controling faith was verified to have a negative effect on cyber bullying, so faith is a very important factor in personalized environment. Thus, it is suggested that the characteristics of cyber space and education of humanism, which can affect individuals' faith should be emphasized.
Gonzalez-Vizcarra, Victor Manuel;Plascencia, Alejandro;Ramos-Avina, Daniel;Zinn, Richard Avery
Asian-Australasian Journal of Animal Sciences
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v.30
no.11
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pp.1563-1567
/
2017
Objective: The influence of substituting steam-flaked corn (SFC) for dry rolled corn (DRC) on feedlot cattle growth performance and dietary net energy when cattle are allowed either ad libitum or 2-h restricted access to the finishing diet was evaluated. Methods: Treatment effects were tested using 96 crossbred steers ($251{\pm}2kg$) during the initial 56 d of the finishing phase. Cattle were blocked by weight and randomly assigned within blocks to 16 pens (4 pens/treatment). Bunk space was sufficient (41 cm/head) to allow all steers access to the feed bunk at the same time. Treatments consisted of two finishing diets containing (dry matter basis) 77.1% corn grain processed by dry rolling (density = 0.50 kg/L) or steam flaking (density = 0.36 kg/L). Cattle were fed twice daily at 06:00 and 14:00 h, allowing for approximately 5% residual. In the case of restricted feeding, steers were allowed access to feeders for 1 h following each feeding, after which residual feed was withdrawn. Results: There were no treatment interactions on dry matter intake (DMI), average daily gain (ADG), gain efficiency (G:F), or dietary net energy (NE). Restricting feed access time reduced (p<0.01) feed intake, and hence, ADG. Substitution of SFC for DRC increased (p<0.01) ADG, feed efficiency (G:F), and estimated dietary NE, without affecting DMI. Based on tabular net energy of maintenance ($NE_m$) value (2.18 Mcal/kg) for DRC, the estimated $NE_m$ value for SFC using the replacement technique, averaged 2.44 Mcal/kg; an improvement of 10.7%. The ratio of observed-to-expected dietary NE was not affected by feed access time. Conclusion: Substitution of SFC for DRC in finishing diets for feedlot cattle enhanced ADG, gain efficiency, and the NE value of the diet. Although restriction of feed access time depressed DMI and ADG, it did not affect the comparative benefit of steam flaking toward enhancement of ADG, G:F, and dietary NE.
This paper proposes a rewriting algorithm of OWL-DL ontology query in SPARQL. Currently, to obtain inference results of given SPARQL queries, Web ontology repositories construct inference ontology models and match the SPARQL queries with the models. However, an inference model requires much larger space than its original base model, and reusability of the model is not available for other inferrable SPARQL queries. Therefore, the aforementioned approach is not suitable for large scale SPARQL query processing. To resolve tills issue, this paper proposes a novel SPARQL query rewriting algorithm that can obtain results by rewriting SPARQL queries and accomplishing query operations against the base ontology model. To achieve this goal, we first define OWL-DL inference rules and apply them on rewriting graph pattern in queries. The paper categorizes the inference rules and discusses on how these rules affect the query rewriting. To show the advantages of our proposal, a prototype system based on lena is implemented. For comparative evaluation, we conduct an experiment with a set of test queries and compare of our proposal with the previous approach. The evaluation result showed the proposed algorithm supports an improved performance in efficiency of the inferrable SPARQL query processing without loss of completeness and soundness.
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