• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1176-1183
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• 2009

Lactobacillus crispatus (L. crispatus) ZJ001 is highly adhesive to epithelial cells and expresses S-layer proteins. In this study, S-S-layer layer genes were sequenced and expressed in E. coli to characterize the function of proteins with this particular strain. L. crispatus ZJ001 harbored two S-layer genes slpA and slpB, and only slpA gene was expressed in the bacterium, as revealed by RT-PCR and immunoassays. The mature SlpA showed 47% amino acid sequence identity to SlpB. The SlpA and SlpB of L. crispatus ZJ001 were highly homologous at the C-terminal region to other Lactobacillus S-layer proteins, but were substantially variable at N-terminal and middle regions. Electron microscopic analysis indicated that His-slpA expressed in E. coli was able to form a sheet-like structure similar to the natural S-layer, but His-slpB formed as disc-like structures. In the cell binding experiments, HeLa cells were able to bind to both recombinant His-slpA and His-slpB proteins to the extent similar to the natural S-layer. The cell binding domains remain mostly in the N-terminal regions in SlpA and SlpB, as shown by high binding of truncated peptides SlpA2-228 and SlpB2-249. Our results indicated that SlpA was active and high binding to HeLa cells, and that the slpA gene could be targeted to display foreign proteins on the bacterial surface of ZJ001 as a potential mucosal vaccine vector.

• Journal of the Korean Wood Science and Technology
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• v.37 no.4
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• pp.364-371
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• 2009

This study was carried out to investigate the bending strength of the Larix glulam beams which were reinforced with CFRP (Carbon fiber reinforced plastic) of which the reinforcement ratios were 0.7% and 2.1% by volume. In the bending test, the rupture shape of the reinforced glulam shows that the reinforced glulam broke firstly in the lowest bottom layer on which tension was loaded, but did not in the upper part reinforced with the CFRP layer. The upper part of the reinforced layer kept strength and did not break when the reinforced glulam broke firstly at the bottom part of the reinforced layer, but broke secondly as loading was increased. In the glulam beams reinforced with CFRP of which the reinforcement ratio was 0.7% by volume, the bending strength of the reinforced beams was increased by 28% at the first break. When beams broke up to the upper part of the reinforced layer, the bending strength of the reinforced beams was increased by 55%, compared to those of control glulam beams. When the glulam beams were reinforced with CFRP of which the reinforcement ratio was 2.1% by volume, the bending strength of the reinforced beams was increased by 77%, compared to those of control glulam beams. The ratio of the height of calculated neutral axis using failure mode recommended by Romani and the height of actual neutral axis using strain gauge was 1.03 and agreed well.

• Journal of Information Display
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• v.6 no.2
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• pp.1-6
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• 2005

We demonstrated stability-enhanced liquid crystal (LC) displays using pixel-isolated LC mode in which LC molecules are isolated in pixel by horizontal polymer layer and vertical polymer wall. The device shows good electro-optic properties against external point or bending pressure due to the polymer structures. The polymer wall acts as supporting structure from mechanical pressure and prevents the cell gap from bending. Moreover, the polymer layer acts as an adhesive to ensure a tight attachment of the two substrates. We present herein various methods for producing polymer structures by using an anisotropic phase separation from LC and polymer composites or patterned micro-structures for stable flexible liquid crystal displays.

• Journal of Electrochemical Science and Technology
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• v.3 no.3
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• pp.143-148
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• 2012

The unique effects of highly conductive conducting polymer/SWNT (single walled carbon nanotube) composite nanoparticles in electric double layer capacitors are studied for the enhancement of the adhesive properties, specific capacitance and power characteristics of the electrode. Because the conducting polymer/SWNT composite material, which is believed to act as a polymer binder, an active material for charge storage and a conducting agent, is well distributed on the activated carbon, greatly enhanced adhesion properties, cell capacitance and power characteristics were obtained.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2002.05a
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• pp.65-68
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• 2002

This study of concrete movement confronting a scope inspects the problems about the waterproofing method and leakage/repairement at present and research the mechanism with the variety of applying examples introducing adhesion and inflation using the flexible type of waterproofing and repairement and the substitution method developed with polymer resign composite. The polymer resign transmitted into adhesion or inflation materials under wet environment absorbs adjoining moisture, wraps impurities of concrete surface. get the waterproofing layer and concrete surface adhere toughly, reorganizes the impaired waterproofing layer and get over the detachment of it from concrete surface.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.04a
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• pp.768-773
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• 1999

This study of concrete movement confronting a scope inspects the problems about the waterproofing method and leakage/repairment at present and research the mechanism with the variety of applying examples introducing adhesion and inflation using the flexible type of waterproofing and repairement and the subsititution method developed with polymer resign composite. The polymer resign transmited into adhesion or inflation materials under wet environment absorbs adjoining moisture, wraps impurities of concrete surface, get the waterproofing layer and concrete surface adhere toughly, reorganize the impaired waterproofing layer and get over the detachment of it from concrete surface.

• Journal of Advanced Marine Engineering and Technology
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• v.15 no.3
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• pp.39-44
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• 1991

The purpose of this investigation is to examine the effects of spraying conditions on the mechanical properties of flame-sprayed titania ceramic coating layer. Titania ceramic was sprayed onto the mild steel substrate under the various spraying conditions. Spraying distances were 100, 150, 200, 250mm respectively and pinch air pressures were 0.2 0.3, 0.4 MPa at each spraying distance. The mechanical properties such as microhardness, adhesive strength and erosion wear resistance were tested for the specimens sprayed under the various spraying conditions above mentioned. The optimum operational conditions obtained through the experimental results were 150mm of spraying distance and 0.3 MPa of pinch air pressure.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.8 s.179
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• pp.2022-2031
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• 2000

Since the surface roughness of adherends affects much the strength of adhesivelybonded joints, the effect of surface roughness on the fatigue life of adhesively bonded tubular single lap joints was investigated analytically and experimentally by fatigue torsion test. The stiffness of the interfacial layer between adherends and adhesive was modeled as a normal statistical distribution function of surface roughness of adherends. From the investigation, it was found that the optimum surface roughness of adherends for the fatigue strength of tubular single lap joints was dependent on bondthickness and applied load.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07a
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• pp.687-691
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• 2005

We demonstrated stability-enhanced liquid crystal (LC) displays using pixel-iosolated LC mode in which LC molecules are isolated in pixel by horizontal polymer layer and vertical polymer wall. The device shows good electro-optic properties with external pressure and bending due to the polymer structures. The polymer wall acts as supporting structure from mechanical pressure and maintains the cell gap from bending. Moreover, the polymer layer acts as adhesive for tight attachment of two substrates. We presented various methods to produce the polymer structures by using anisotropic phase separation from LC and polymer composites or patterned micro-structures.

• Journal of the Korean institute of surface engineering
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• v.31 no.2
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• pp.91-100
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• 1998

NiCrAlY/YSZ(8wt%$Y_2O_3-ZrO_2$) functionally graded thermal barrier coating (FGC) layers on a Co-base superalloy (HAYNESS 188) substrate were fabstrate were fabicated using Ar shielded single torch air plasma spraying method. Functional grading with the stepwise compositional change throughout layer thickness. Microstructural observation trvealed a successful fabrication of NiCrAlY/YSZ FGC. From the results of the curvature measurement, adhesive strength measurement and thermal shock test for the FGC, it was concluded that the optimum conditions of functionally graded coating layer thinkness and compositional pattern exit to enhance the properties of FGC, which is closely related to the internal residual stess distribution witin it.