• Journal of Marine Bioscience and Biotechnology
• /
• v.1 no.4
• /
• pp.260-267
• /
• 2006

The Liquid Culture Assay using a recombinant Agrobacterium tumefaciens strain has been developed as a means for quorum sensing autoinducer screening. However, the low aqueous solubility of marine natural product extracts used as potential autoinducers has been a hindrance in the screening process. Although the addition of organic solvents and/or surfactants could increase aqueous solubility, errors in data interpretation including false positive results could be a serious problem. Therefore, determining the best possible solvent and surfactant at the optimum concentration is crucial. Evaluating methanol, ethanol, 1-propanol, DMSO and DMF at concentration ranges of 0~10% revealed < 2% methanol to be most favorable when tested for ${\beta}$-gal activity and growth inhibition of the recombinant A. tumefaciens strain. On the other hand, among surfactants tested, Triton X-100 was similarly effective in increasing the delivery of autoinducers for activity at less than 0.05% concentration.

• Geophysics and Geophysical Exploration
• /
• v.7 no.1
• /
• pp.93-98
• /
• 2004

The sand-covered Muweilah archaeological site in the United Arab Emirates (UAE) is a unique Iron Age site, and has been subject to intensive investigations. However, excavations are time consuming and may require twenty years to complete. Thus geophysical surveys were undertaken with the objective of characterising the site more expeditiously. This paper presents preliminary results of these surveys. Ground penetrating radar (GPR) was tested as a primary imaging tool, with an ancillary shallow time domain EM (MetalMapper) system. Dense 3D GPR datasets were migrated to produce horizontal (plan view) depth slices at 10 cm intervals, which is conceptually similar to the archaeologists' excavation methodology. The objective was to map all features associated with anthropogenic activity. This required delineating extensive linear and planar features, which could represent infrastructure. The correlation between these and isolated point reflectors, which could indicate anthropogenic activity, was then assessed. Finally, MetalMapper images were used to discriminate between metallic and non-metallic scatterers. The moderately resistive sand cover allowed GPR depth penetration of up to 5 m with a 500 MHz system. GPR successfully mapped floor levels, walls, and isolated anthropogenic activity, but crumbling walls were difficult to track in some cases. From this study, two possible courtyard areas were recognised. The MetalMapper was less successful because of its limited depth penetration of 50 cm. Despite this, the system was still useful in detecting modem-day ferruginous waste and bronze artefacts. The results (subject to ongoing ground-truthing) indicated that GPR was optimal for sites like Muweilah, which are buried under a few metres of sand. The 3D survey methodology proved essential to achieve line-to-line correlation for tracking walls. In performing the surveys, a significant improvement in data quality ensued when survey areas were flattened and de-vegetated. Although MetalMapper surveys were not as useful, they certainly indicated the value of including other geophysical data to constrain interpretation of complex GPR features.

• Translational and Clinical Pharmacology
• /
• v.25 no.3
• /
• pp.147-152
• /
• 2017

This study focused on the role of cytochrome P450 2D6 (CYP2D6) genotypes to predict phenotypes in the metabolism of dextromethorphan. CYP2D6 genotypes and metabolic ratios (MRs) of dextromethorphan were determined in 201 Koreans. Unsupervised clustering algorithms, hierarchical and k-means clustering analysis, and color visualizations of CYP2D6 activity were performed on a subset of 130 subjects. A total of 23 different genotypes were identified, five of which were observed in one subject. Phenotype classifications were based on the means, medians, and standard deviations of the log MR values for each genotype. Color visualization was used to display the mean and median of each genotype as different color intensities. Cutoff values were determined using receiver operating characteristic curves from the k-means analysis, and the data were validated in the remaining subset of 71 subjects. Using the two highest silhouette values, the selected numbers of clusters were three (the best) and four. The findings from the two clustering algorithms were similar to those of other studies, classifying $^*5/^*5$ as a lowest activity group and genotypes containing duplicated alleles (i.e., $CYP2D6^*1/^*2N$) as a highest activity group. The validation of the k-means clustering results with data from the 71 subjects revealed relatively high concordance rates: 92.8% and 73.9% in three and four clusters, respectively. Additionally, color visualization allowed for rapid interpretation of results. Although the clustering approach to predict CYP2D6 phenotype from CYP2D6 genotype is not fully complete, it provides general information about the genotype to phenotype relationship, including rare genotypes with only one subject.

• The Journal of the Korea Contents Association
• /
• v.22 no.6
• /
• pp.369-385
• /
• 2022

This thesis was a phenomenological study of an after-school picture book making program for middle school students. This study attempted to establish a theoretical system for picture book making activity by analyzing the process of self-discovery and healing through the picture book making activity. The participants were six middle school students, the picture book making activity program was organized into five stages, and in-depth interviews of the study participants and their interpretation of picture books were described. According to the research results, first, the making activity of picturebooks can construct one's own world through hyper-reality that is more excessive than the original. Second, it can be said that the fearful existence of a monster has developed awareness and insight to view its own problems from various angles through defamiliarization. These results might be that the making activity of picturebooks is a way of revealing one's existence to the world, and through this, the process of exploring and constructing the meaning of oneself and daily life. Discussion was made based on this about the creation of another world where the picturebook making activity is different from the original, the stimulating effect of the subconscious mind through the defamiliarization device, and the establishment of self-existence in picturebook creation.

• Journal of radiological science and technology
• /
• v.29 no.4
• /
• pp.249-255
• /
• 2006

Purpose: The diagnostic utility of fluorine-18 2-deoxy-D-glucose positron emission tomograhpy ($^{18}F-FDG $PET) for the non-invasive differentiation of focal lung lesions originated from cancer or inflammation disease by combined visual image interpretation and semi-quantitative uptake value analysis has been documented. In general, Standardized Uptake Value(SUV) is used to diagnose lung disease. But SUV does not contain dynamic information of lung tissue for the glucose. Therefore, this study was undertaken to hypothesis that analysis of dynamic kinetics of focal lung lesions base on $^{18}F-FDG$ PET may more accurately determine the lung disease. So we compared Time Activity Curve(TAC), Standardized Uptake Value-Dynamic Curve(SUV-DC) graph pattern with Glucose Metabolic Rate(MRGlu) from Patlak analysis. Methods: With lung disease, 17 patients were examined. They were injected with $^{18}F-FDG$ over 30-s into peripheral vein while acquisition of the serial transaxial tomographic images were started. For acquisition protocol, we used twelve 10-s, four 30-s, sixteen 60-s, five 300-s and one 900-s frame for 60 mins. Its images were analyzed by visual interpretation TAC, SUV-DC and a kinetic analysis(Patlak analysis). The latter was based on region of interest(ROIs) which were drawn with the lung disease shape. Each optimized patterns were compared with itself. Results: In TAC patterns, it hard to observe cancer type with inflammation disease in early pool blood area but over the time cancer type slope more remarkably increased than inflammation disease. SUV-DC was similar to TAC pattern. In the result of Patlak analysis, In time activity curve of aorta, even though inflammation disease showed higher blood activity than cancer, at first as time went by, blood activity of inflammation disease became the lowest. However, in time activity curve of tissue, cancer had the highest uptake and inflammation disease was in the middle. Conclusion: Through the examination, TAC and SUV-DC could approached the results that lung cancer type and inflammation disease type has it's own difference shape patterns. Also, it has outstanding differentiation between cancer type and inflammation in Patlak and MRGlu analysis. Through these analysis methods, it will helpful to separation lung disease.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.18 no.1
• /
• pp.3-14
• /
• 1994

This study is intended to identify the clothing mage as clothing form and dot space variation. This study consists of pre-experiment for setting the space between dot which shows the difference of the image and main experiment and also is made of factorial design for two variables-clothing form(H-line, A-line, V-line, X-line) dot (dot 1.0, dot 2.2, dot 3.4, dot 5.8) Qestionaire consists of 21 semantic differential scale expressing clothing form and .dot. Data is obtained from 50 female students maforing clothing and textile. The results of this study are as follows. 1) The image by the clothing form and the space dot variation is composed 5 factors' attention, activity, attraction, maturity, boldness. 2) The image by variation in clothing form dot has significant differences in attractive and attention factors, especially shows remarkable differences in attention. By choosing narrow space dot for attentive image, broden space dot for attractive image, you would be able to create the image you want. 3) The image by variation in dot clothing form has almost signific and differences in all factors, especially shows remarkable differences in activity. By choosing A-line for active image, V-line for mature and fashionable image, X-line for attractive, pretty and delicate mage, you would be able to create the image you want. 4) The interaction effect between clothing form and space dot was in attraction and maturity factor, V-line, X-line, dot 3.4 and dot 5.8 intensify attractive image, V-line and dot 1.0 mature image, X-line and dot 5.8 young image.

• Journal of the Korean Chemical Society
• /
• v.29 no.4
• /
• pp.348-355
• /
• 1985

Extended Huckel Calculation of cis-and trans-dichloro diammine platinum(II), dichloroethylene diamine platinum(II) and their N-mono, di, tri and tetra-methylated or ethylated ones were carried out to investigate their anticancer activity. It was found that the net charge of two chlorine atoms in cis-isomers are greater than those in trans-ones and Pt-Cl bond energies of the former are less than that of the latter, indicating that Pt-Cl bond in cis-isomers has greater ionic character than that in trans-ones and Cl atoms in the former are easier to dissociated as Cl- than those in the latter. Also, the values of $b_{2g}-b_{1g}$ energy difference, ${\Delta}_1$ were found to be greater in cis-isomers than in trans-one without exceptions. For the substitution of methyl for H atom in ammine and ethylenediamine Pt-Cl bond strength shows the tendency to increase with increasing in number of methyl group. Accordingly, We believe that two Cl atoms in $PtLCl_2$-complexes (L: NH$_3$, en) are dissociated in the first step of the action of anticancer.

• The Journal of the Korea Contents Association
• /
• v.13 no.12
• /
• pp.869-879
• /
• 2013

Modern medicine has not yet conquered behavior therapy for cancer radiation treatment, which is one of the professional groups involved in the justification of the act and reasonable human resources was analyzed. Radiation Oncology(Therapeutic Radiology) installed the role of the medical physicist in the current law, the act must have been within the limits given licenses, but the legitimacy of the act which can be given the current laws and regulations are not clear. Thus, certification and testing outside the medical physicist's research institutions, including the measures to be reflected in national legislation sought. Medical physicists, with the inherent illegality act because one of the areas to precluding the illegality of the content-based "medical law" and "Nuclear Safety Law", "medical technology in the field of radiation safety standards on management" was based on the interpretation. In conclusion, "medical law" and "in the field of medical technology on the management of radiation safety standards" that are consistent with the recognition of qualifications, increased activity in the radiation therapy and radiation safety management must deal with this will be feasible.

• Journal of Applied Biological Chemistry
• /
• v.54 no.2
• /
• pp.114-119
• /
• 2011

n-Butanol extracts from Phellodendron amurense have about 50% inhibitory activity against hyaluronidase. The anti-inflammatory compound was isolated from P. amurense by Sephadex LH-20 and MCI-gel CHP-20 column chromatography with gradient elution. As a the result, its structure was identified as Jatrorrhizine by the interpretation of spectroscopic analyses including $^1H$- and $^{13}C$-NMR. In anti-inflammatory activity, the expression of nitric oxide (NO) was inhibited as above 60% at 100 ${\mu}g/mL$ concentration of extracts and then purified Jatrorrhizine from P. amurense. The inhibitory activities against the expression of inducible NO syntase (iNOS) and cyclooxygenase-2 (COX-2) were 45% and 29%. It seems that the extracts and purified Jatrorrhizine from P. amurense were expected anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated Raw264.7 cells.

• BMB Reports
• /
• v.33 no.3
• /
• pp.195-201
• /
• 2000

A human gene has been reported that may encode the enzyme acetohydroxyacid synthase. Previously this enzyme was thought to be absent from animals although it is present in plants and many microorganisms. In plants, this enzyme is the target of a number of commercial herbicides and the use of these compounds may need to be reassessed if the human enzyme exists and proves to be susceptible to inhibition. Here we report the construction of several plasmid vectors containing the cDNA sequence for this protein, and their expression in Escherichia coli. High levels of expression were observed, but most of the protein proved to be insoluble. The small amounts of soluble protein contained little or no acetohydroxyacid synthase activity. Attempts to refold the insoluble protein were successful insofar as the protein became soluble. However, the refolded protein did not gain any acetohydroxyacid synthase activity. In vivo complementation tests of an E. coli mutant produced no evidence that the protein is active. Incorrect folding, or the lack of another subunit, may explain the data but we favor the interpretation that this gene does not encode an acetohydroxyacid synthase.