• Atmosphere
• /
• v.31 no.1
• /
• pp.17-28
• /
• 2021

We investigated the impact of domain size on the simulated summer precipitation over the Korean Peninsula using the Weather Research and Forecasting (WRF) model. Two different domains are integrated up to 72-hours from 29 June 2017 to 28 July 2017 when the Changma front is active. The domain sizes are adopted from previous RDAPS (Regional Data Assimilation and Prediction System) and current LDAPS (Local Data Assimilation and Prediction System) operated by the Korea Meteorological Administration, while other model configurations are fixed identically. We found that the larger domain size showed better prediction skills, especially in precipitation forecast performance. This performance improvement is particularly noticeable over the central region of the Korean Peninsula. Comparisons of physical aspects of each variable revealed that the inflow of moisture flux from the East China Sea was well reproduced in the experiment with a large model domain due to a more realistic North Pacific high compared to the small domain experiment. These results suggest that the North Pacific anticyclone could be an important factor for the precipitation forecast during the summer-time over the Korean Peninsula.

• Microbiology and Biotechnology Letters
• /
• v.24 no.3
• /
• pp.290-298
• /
• 1996

In an effort to understand the role of the conserved domain and of the heterologous one-third part of the carboxy terminal domain of transglutaminase C (TGase II), attempts were made to express TGase II cDNA of human origin in yeast Saccharomyces cerevisiae as in a full-length form as well as in a form of C-terminal truncation. The 2$\mu$-based expression plasmids which contained the TGase II cDNA under the gal inducible promoter were introduced into yeast and the maintenance of the full-length and truncated form of the TGase II gene plasmids were confirmed by Southern blot. The expression of the TGase II gene was analysed by reverse transcription polymerase chain reaction (RT-PCR), and western blot analyses. As assayed by [1,4$^{14}$C]-putrescine incorporation into succinylated casein, the full-lenth as well as the truncated forms of recombinant TGase II showed some catalytic activity. These results indicate that the N-terminal homologous domain of human TGase II retains a catalytically active domain. The level of TGase II expressed in yeast, however, was far lower than satisfactory and other expression system should be sought further chracterization of the enzyme. The negative effect of TGase II on the growth of yeast is interesting with respect to the physiological effect of TGase II in cornification of epidermal keratinocytes.

• BMB Reports
• /
• v.32 no.3
• /
• pp.215-225
• /
• 1999

Phytochromes (with gene family members phyA, B, C, D, and E) are a wavelength-dependent light sensor or switch for gene regulation that underscore a number of photo responsive developmental and morphogenic processes in plants. Recently, phytochrome-like pigment proteins have also been discovered in prokaryotes, possibly functioning as an auto-phosphorylating/phosphate-relaying two-component signaling system (Yeh et al., 1997). Phytochromes are photochromically convertible between the light sensing Pr and regulatory active Pfr forms. Red light converts Pr to Pfr, the latter having a "switch-on" conformation. The Pfr form triggers signal transduction pathways to the downstream responses including the expression of photosynthetic and other growth-regulating genes. The components involved in and the molecular mechanisms of the light signal transduction pathways are largely unknown, although G-proteins, protein kinases, and secondary messengers such as $Ca^{2+}$ ions and cGMP are implicated. The 124-127 kDa phytochromes form homodimeric structures. The N-terminal half contains the tetrapyrrolic phytochromobilin for red/far-red light absorption. The C-terminal half includes both a dimerization motif and regulatory box where the red light signal perceived by the chromophore-domain is recognized and transduced to initiate the signal transduction cascade. A working model for the inter-domain signal communication within the phytochrome molecule is proposed in this Review.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2005.11a
• /
• pp.620-628
• /
• 2005

Spatial control of sound is essential to deliver better sound to the listener's position in space. As it can be experienced in many listening environments, the quality of sound can not be manifested over every position in a hall. This motivates us to control sound in a region we select. The primary focus of the developed method has to do with the brightness and contrast of acoustic image in space. In particular, the acoustic brightness control seeks a way to increase loudness of sound over a chosen area, and the contrast control aims to enhance loudness difference between two neighboring regions. This enables us to make two different kinds of zone - the zone of quiet and the zone of loud sound - at the same time. The other perspective of this study is on the direction of sound. It is shown that we can control the direction of perceived sound source by focusing acoustic energy in wavenumber domain. To begin with, the proposed approaches are formulated for pure-tone case. Then the control methods are extended to a more general case, where the excitation signal has broadband spectrum. In order to control the broadband signal in time domain, an inverse filter design problem is defined and solved in frequency domain. Numerical and experimental results obtained in various conditions certainly validate that the acoustic brightness, acoustic contrast, direction of wave front can be manipulated for some finite region in space and time.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.14 no.12
• /
• pp.4725-4747
• /
• 2020

Software defined networking (SDN) is considered to be one of the most promising paradigms in the future. To solve the scalability and performance problem that a single and centralized controller suffers from, the distributed multi-controller architecture is adopted, thus forms multi-domain SDN. In a multi-domain SDN network, it is of great importance to ensure a reliable control plane. In this paper, we focus on the reliability problem of multi-domain SDN against controller failure from perspectives of backup controller deployment and controller replication. We firstly propose a placement algorithm for backup controllers, which considers both the reliability and the cost factors. Then a controller replication mechanism based on shared data storage is proposed to solve the inconsistency between the active and standby controllers. We also propose a shared data storage layout method that considers both reliability and performance. Besides, a fault recovery and repair process is designed based on the controller backup and shared data storage mechanism. Simulations show that our approach can recover and repair controller failure. Evaluation results also show that the proposed backup controller placement approach is more effective than other methods.

• BMB Reports
• /
• v.32 no.4
• /
• pp.358-362
• /
• 1999

dTDP-D-glucose 4,6-dehydratase as an oxidoreductase catalyzes the conversion of dTDP-D-glucose to dTDP-4-keto-6-deoxy-D-glucose, which is essential for the formation of 6-deoxysugars. dTDP-D-glucose 4,6-dehydratase shows remarkable sterochemical convergence in which displacement of the C-6 hydroxyl group by a C-4 hydrogen proceeds intramolecularly with inversion of configuration. The reaction mechanism is known to be oxidation, dehydration, and reduction by bases mediating proton transfer and $NAD^+$ cofactor. In this study, the bases in the active site domain are proposed to be His-79 and His-300 from a comparison of the peptides of the dehydratase and UDP-D-glucose epimerase. His-79 and His-300 were mutated to prepare the mutants H79L (mutation of histidine to leucine at the 79th amino acid) and H300A (mutation of histidine to alanine at the 300th amino acid) by site-directed mutagenesis. The H79L protein was inactive, showing that His-79 participates in the reaction mechanism.

• Proceedings of the KIEE Conference
• /
• 2011.07a
• /
• pp.145-146
• /
• 2011

A micro-grid (MG) is usually interconnected to the main grid through the dedicated line. Immediately after the removal of the grid supply, the MG should be disconnected and remain disconnected until the main grid is re-energized. It should detect islanding condition as soon as it happens to adjust the setting of the protection relays in the MG. This paper proposes an islanding detection algorithm for the MG based on the active and reactive power delivered to the dedicated line in the time domain. The performance of the proposed algorithm is verified under islanding conditions and fault conditions using the PSCAD/EMTDC simulator. The results indicate that the proposed algorithm can discriminate the islanding conditions from the various fault conditions.

• ALGAE
• /
• v.27 no.1
• /
• pp.55-62
• /
• 2012

A novel lectin specific to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine was isolated from Bryopsis plumosa and named as BPL-4. Sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE) and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) mass spectrometry data showed that this lectin was a monomeric protein with molecular weight 12.9 kDa. The N-terminal amino acid sequences of the lectin were determined by Edman degradation and the full cDNA sequence encoding this lectin was obtained using the degenerate primers designed from the amino acid sequence. The size of the cDNA was 414 bp containing single open reading frame (ORF) encoding the lectin precursor. The homology analysis showed that this lectin might belong to H lectin group. BPL-4 showed high sequence similarity (60.6%) to BPL-3, which is a previously reported lectin from the same species. The comparative analysis on the lectin's primary structure showed two conserved domains including one possible active domain of H lectin group.

• The Journal of the Acoustical Society of Korea
• /
• v.35 no.1
• /
• pp.8-15
• /
• 2016

As a generalized form of the conventional Fourier transform, fractional Fourier transform (FrFT) can analyze a signal at intermediate domain between time and frequency domains with a transform order ${\alpha}$. Especially, FrFT has a number of advantages in the analysis of LFM (Linear Frequency Modulation) signals due to its robustness to noise. In this paper, we have proposed a new method to detect and estimate the distance of the target from the FrFT spectrum of the received echo signal. Experimental results have validated the proposed method, and shown that reliable target distance could be estimated in noise and reverberation environments.

• Proceedings of the Korea Crystallographic Association Conference
• /
• 2002.11a
• /
• pp.24-24
• /
• 2002

HtrA (high temperature requirement A), a periplasmic heat shock protein, is known to have molecular chaperone function at low temperatures and proteolytic activity at elevated temperatures. To investigate the mechanism of functional switch to pretense, we have determined the crystal structure of the N-terminal protease domain (PD) of HtrA from Thermotoga maritima. HtrA PD shares the same fold with chymotrypsin-like serine professes. However, crystal structure suggests that HtrA PD is not an active pretense at current state since its active site is not formed properly and blocked by an additional helical lid. On the surface of the lid, HtrA PD has hydrophobic patches that could be potential substrate binding sites for molecular chaperone activity. Present structure suggests that the activation of the proteolytic function of HtrA PD at elevated temperatures might occur by the conformational change.